Agronomic Effects of Foliar Calcium Sprays on Chili Peppers
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摘要:
目的 通过叶面喷施不同钙源研究其对辣椒的农艺性状、果实钙含量的影响,为生产上辣椒果实钙含量的提升、产量增加和品质改善提供参考。 方法 以辣椒为试验材料,采用基质培养,设置CK(叶面喷施清水)、G1(叶面喷施氯化钙)、G2(叶面喷施糖醇钙)、G3(叶面喷施小分子肽钙)4个处理,测定辣椒的主要农艺性状、钙含量、产量和品质等指标。 结果 喷施3种不同的钙源均可加快辣椒果实的成熟,提高地上部钙素在果实的分配率。G3与CK相比,辣椒的地上部鲜重显著增加22.02%,单株产量为536.9 g,显著增产38.77%。果实钙含量与积累量分别达2.78 g·kg−1和96.86 g·株−1,较CK处理显著升高46.32%和78.25%。辣椒地上部中,果实的钙素分配率较CK处理显著提高58.58%,维生素C含量达0.6378 mg·g−1,较CK处理显著增加17.29%;G3与G1相比,辣椒的单株产量、单果重和维生素C含量分别显著提高18.0%、33.8%、18.3%;G3与G2相比,辣椒的地上部鲜重、成熟果个数和单株产量均显著提高。地上部氮、钾、钙的积累量增加。 结论 与氯化钙和糖醇钙相比,小分子肽钙能更好地从辣椒的叶片转移到果实中,进而提高果实钙含量。供试条件下,在促进辣椒生长、提高产量和品质方面,叶面喷施小分子肽钙的处理效果最佳。 Abstract:Objective Effects of foliar spraying different calcium solutions on the agronomic traits of chili pepper were studied for crop yield and quality improvements. Method Four foliar spraying treatments using water (CK) or solutions of calcium chloride (G1), calcium sugar alcohol (G2), and small molecular peptide calcium (G3) were performed on chili pepper plants. Major agronomic characteristics, calcium content, yield, and quality of the plants were determined. Result The various calcium sprays hastened the pepper ripening and increased the fruit calcium content. Compared with CK, the G3-treated peppers increased significantly by 22.02% on fresh weight and by 38.77% at 536.9 g on yield per plant. Meanwhile, the content and the accumulation of calcium in the fruit reached 2.78 g·kg−1 and 96.86 g per plant, representing significant increases over CK by 46.32% and 78.25%, respectively. In the aboveground pepper plant, the fruits had a significantly increased share of calcium by 58.58% and Vitamin C (0.6378 mg·g−1) by 17.29% over those of CK. The G3-treated peppers also had higher yield per plant, fruit weight, and Vitamin C by 18.0%, 33.8%, and 18.3%, respectively, than the G1-treated ones, and significantly higher fresh shoot weight, mature fruit count, and yield per plant than the G2 counterparts. The sprays also resulted in more accumulations of nitrogen, potassium, and calcium in the aboveground parts of the plants. Conclusion The small molecule peptide calcium used in the foliar spraying was more efficient than the other forms in transferring calcium from the leaves to the fruits of a chili pepper plant. It was recommended for promoting the plant growth as well as increasing the yield and nutritional quality of chili peppers. -
Key words:
- Chili pepper /
- small molecular peptide calcium /
- calcium content /
- fruit quality
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图 2 叶面喷施不同钙肥对辣椒各部位大量元素与钙素含量的影响
不同小写字母代表同一部位不同处理之间在5%水平差异显著,下同。
Figure 2. Effects of foliar calcium sprays on macroelements and calcium in aboveground chili pepper plant
The same lowercase letters represent significant differences of different treatments at the same pepper part at the 5% level, the same below.
表 1 试验处理
Table 1. Experimental treatments
处理
Treatments叶面喷施液成分
Foliar spray composition肥料含量
Fertilizer content/(g·L−1)钙素浓度
Calcium concentration/(mol·L−1)CK 清水 — 0.00 G1 氯化钙 1.12 0.01 G2 糖醇钙 4.20 0.01 G3 小分子肽钙 5.20 0.01 每升体积肥料液配完后再加入表面活性剂2 mL(吐温20),方便肥液铺展在叶片上。
2 mL surfactant (Tween 20) were added in a liter of spraying solution to facilitate spreading on leaves.表 2 叶面喷施不同钙肥对辣椒生长指标与干物质的影响
Table 2. Effects of foliar calcium sprays on growth and dry matters of chili peppers
处理
Treatments株高
Plant
height/cm茎粗
Stem
diameter/mm地上部鲜重
FW of above
ground/(g·株−1)果干重
Friut DW/
(g·株−1)叶干重
Leaf DW/
(g·株−1)茎干重
Stem DW/
(g·株−1)地上部干重
DW of above ground/
(g·株−1)CK 83.4 ± 7.9a 11.4 ± 1.3a 604.8 ± 16.9b 28.4 ± 0.9b 18.4 ± 0.2a 14.4 ± 1.0a 61.2 ± 1.7a G1 77.7 ± 5.2a 11.6 ± 0.1a 666.6 ± 12.1ab 33.3 ± 0.9ab 19.7 ± 0.9a 14.0 ± 1.3a 66.9 ± 2.1a G2 75.8 ± 8.5a 11.9 ± 1.0a 604.8 ± 20.1b 28.1 ± 1.8b 18.2 ± 1.9a 13.1 ± 2.3a 59.4 ± 5.0a G3 71.7 ± 3.6a 11.0 ± 0.1a 738.0 ± 37.9a 34.7 ± 2.4a 17.6 ± 0.5a 12.3 ± 1.8a 64.6 ± 4.2a FW表示鲜重,DW表示干重t。表中数据为“平均值 ± 标准误”。同列数字后存在相同小写字母代表在5%水平上差异不显著,下同。
FW: fresh weight; DW: dry weight. Data are mean ± standard error; those with same lowercase letters on same column indicate no significant differences at 5% level. Same for below.表 3 叶面喷施不同钙肥对辣椒农艺性状与坐果率的影响
Table 3. Effects of foliar calcium sprays on agronomic traits and fruit set rate of chili peppers
喷施完成后时间
Days after spraying/d处理
Treatments成熟果数
Ripe fruit per plant结果数
Fruits per plant开花数
Flowers per plant坐果率
Fruit set rate/%7 CK — 7.0 ± 0.0b 13.3 ± 1.2a — G1 — 8.7 ± 0.3b 16.7 ± 0.3a — G2 — 6.0 ± 0.0b 16.0 ± 1.5a — G3 — 11.7 ± 1.7a 16.7 ± 1.5a — 14 CK 2.0 ± 0.6c 15.3 ± 3.4a 10.3 ± 1.7a — G1 4.0 ± 0.6b 20.0 ± 3.1a 7.3 ± 0.9a — G2 4.0 ± 0.6b 18.7 ± 5.4a 6.7 ± 2.9a — G3 6.7 ± 0.3a 23.3 ± 4.6a 7.7 ± 2.3a — 21 CK 2.7 ± 0.3c 20.0 ± 3.5a 6.7 ± 2.7a 47.7 ± 3.3a G1 5.7 ± 0.3ab 27.7 ± 4.8a 3.0 ± 1.0a 59.4 ± 2.9a G2 4.7 ± 0.9b 21.7 ± 6.4a 2.3 ± 0.3a 57.1 ± 6.4a G3 7.3 ± 0.7a 27.7 ± 4.5a 2.0 ± 0.6a 52.8 ± 4.5a 表 4 叶面喷施不同钙肥对辣椒单株产量、单果质量和果实大小的影响
Table 4. Effects of foliar calcium sprays on yield per plant, single fruit weight, and fruit size of chili peppers
处理
Treatments单株产量
Yield per plant/g单果重
Single fruit weight/g果长
Fruit length/cm果横径
Fruit width/mmCK 386.9±13.9c 47.8±2.6c 14.0±0.3b 35.7±0.8ab G1 455.0±14.4b 54.5±3.7bc 16.9±0.6a 34.1±1.5b G2 398.4±20.4bc 66.4±5.5ab 17.0±0.9a 37.4±2.6ab G3 536.9±26.1a 72.9±4.9a 17.6±0.5a 41.1±0.7a 表 5 叶面喷施不同钙肥对辣椒各部位大量元素与钙素积累量的影响
Table 5. Effects of foliar calcium sprays on macroelements and calcium accumulation in parts of chili pepper plant
部位
Part处理
Treatments氮积累量
N accumulation/(g·株−1)磷积累量
P accumulation/(g·株−1)钾积累量
K accumulation/(g·株−1)钙积累量
Ca accumulation/(g·株−1)果实
FruitCK 675.49±22.09 a 48.07±1.57 b 669.80±21.90 b 54.34±4.85 b G1 758.19±19.83 a 73.83±1.93 a 800.75±20.95 ab 75.94±5.76 ab G2 691.51±43.94 a 49.74±3.16 b 686.17±43.61 b 73.89±12.41 ab G3 786.89±54.23 a 51.07±3.52 b 838.66±57.80 a 96.86±9.10 a 叶片
LeafCK 601.77±7.96 b 37.83±0.50 b 533.27±7.05 a 505.64±23.53 b G1 702.03±31.62 ab 64.97±2.93 a 524.25±23.61 a 667.12±40.66 a G2 766.41±81.47 a 35.62±3.79 b 563.63±59.91 a 527.87±70.34 ab G3 745.18±22.35 ab 39.42±1.18 b 479.78±14.39 a 538.34±22.19 ab 茎秆
StemCK 283.18±19.75 a 19.67±1.37 a 452.48±31.55 a 115.52±6.72 a G1 229.71±21.52 a 27.28±2.55 a 419.84±39.33 a 115.00±13.88 a G2 241.52±42.88 a 21.41±3.80 a 370.23±65.73 a 123.74±18.06 a G3 216.11±31.53 a 18.70±2.73 a 326.93±47.71 a 124.49±18.14 a 地上部
Pepper shootsCK 1 560.44±40.15 a 105.57±2.77 b 1 655.55±48.22 a 675.50±32.55 a G1 1 689.93±53.12 a 166.08±5.33 a 1 744.84±58.31 a 858.06±41.82 a G2 1 699.44±144.48 a 106.77±8.91 b 1 620.03±141.62 a 725.50±87.88 a G3 1 748.18±94.64 a 109.19±6.53 b 1 645.37±105.62 a 759.69±31.44 a -
[1] 王立浩, 马艳青, 张宝玺. 我国辣椒品种市场需求与育种趋势 [J]. 中国蔬菜, 2019(8):1−4.WANG L H, MA Y Q, ZHANG B X. Market demand and breeding trend of Chili varieties in China [J]. China Vegetables, 2019(8): 1−4.(in Chinese) [2] 王立浩, 张正海, 曹亚从, 等. “十二五”我国辣椒遗传育种研究进展及其展望 [J]. 中国蔬菜, 2016(1):1−7. doi: 10.3969/j.issn.1000-6346.2016.01.001WANG L H, ZHANG Z H, CAO Y C, et al. Research progress and prospect of pepper genetic breeding in ' Twelfth Five-Year ' in China [J]. China Vegetables, 2016(1): 1−7.(in Chinese) doi: 10.3969/j.issn.1000-6346.2016.01.001 [3] 李玉鹏, 杨光, 李飞, 等. 糖醇螯合钙肥对马铃薯产量、品质及养分吸收的影响 [J]. 土壤, 2020(4):773−780. doi: 10.13758/j.cnki.tr.2020.04.017LI Y P, YANG G, LI F, et al. Effects of sugar alcohol chelated calcium fertilizer on yield, quality and nutrient uptake of potato [J]. Soils, 2020(4): 773−780.(in Chinese) doi: 10.13758/j.cnki.tr.2020.04.017 [4] 王云堂. 钙在植物营养中的作用分析 [J]. 种子科技, 2019, 37(6):32−33. doi: 10.3969/j.issn.1005-2690.2019.06.031WANG Y T. Analysis of the Role of Calcium in Plant Nutrition [J]. Seed Science & Technology, 2019, 37(6): 32−33.(in Chinese) doi: 10.3969/j.issn.1005-2690.2019.06.031 [5] 杨海波, 周鹏程, 孟利峰. 果树叶面喷钙的研究进展 [J]. 现代园艺, 2012(5):11−12. doi: 10.3969/j.issn.1006-4958.2012.05.008YANG H B, ZHOU P C, MENG L F. Research Progress on Spraying Calcium on Fruit Leaves [J]. Xiandai Horticulture, 2012(5): 11−12.(in Chinese) doi: 10.3969/j.issn.1006-4958.2012.05.008 [6] 唐宽强, 刘守伟, 吴凤芝, 等. 外源喷施CaCl2对低温逆境下番茄抗冷性及开花结果的影响 [J]. 北方园艺, 2013(11):10−14.TANG K Q, LIU S W, WU F Z, et al. Effect of exgenous CaCl2 on the cold resistance and blossom and yield of tomato under cold stress [J]. Northern Horticulture, 2013(11): 10−14.(in Chinese) [7] 李石开, 陶婧, 桂敏, 等. 氯化钙和多效唑浸种对干制辣椒种子发芽及幼苗抗旱性的影响 [J]. 西南农业学报, 2012, 25(5):1786−1789. doi: 10.3969/j.issn.1001-4829.2012.05.051LI S K, TAO J, GUI M, et al. Effects of seed soaking with CaCl2 and PP333 on germination and seedling drought resistance in dry chili [J]. Southwest China Journal of Agricultural Sciences, 2012, 25(5): 1786−1789.(in Chinese) doi: 10.3969/j.issn.1001-4829.2012.05.051 [8] 姜伟, 王勇, 曹继龙, 等. 日光温室番茄需肥特点及其科学施肥技术研究 [J]. 内蒙古农业科技, 2012(5):56−58.JIANG W, WANG Y, CAO J L, et al. The greenhouse tomato fertilizer requirement features and scientific fertilization technology [J]. Inner Mongolia Agricultural Science and Technology, 2012(5): 56−58.(in Chinese) [9] 梁怡, 卢明, 姚智, 等. 含钙镁辣椒配方肥对辣椒生产及品质的影响 [J]. 中国土壤与肥料, 2021(1):109−116. doi: 10.11838/sfsc.1673-6257.19572LIANG Y, LU M, YAO Z, et al. Effect of formula fertilizer containing calcium and magnesium on production and quality of pepper [J]. Soil and Fertilizer Sciences in China, 2021(1): 109−116.(in Chinese) doi: 10.11838/sfsc.1673-6257.19572 [10] 任朝辉, 田旭芳, 廖卫琴, 等. 不同辣椒种质资源的品质性状评价 [J]. 西南农业学报, 2020(9):1884−1891.REN C H, TIAN X F, LIAO W Q, et al. Evaluation on quality characters of different pepper germplasm resources [J]. Southwest China Journal of Agricultural Sciences, 2020(9): 1884−1891.(in Chinese) [11] 陈龙涛, 樊明寿, 王迎男, 等. 喷施不同浓度钙试剂对花后榛子果实生长及结实率的影响 [J]. 东北林业大学学报, 2018(6):33−37. doi: 10.3969/j.issn.1000-5382.2018.06.007CHEN L T, FAN M S, WANG Y N, et al. Effects of spraying calcium reagent in different contents after flowering on the growth and setting rate of hazelnut fruits [J]. Journal of Northeast Forestry University, 2018(6): 33−37.(in Chinese) doi: 10.3969/j.issn.1000-5382.2018.06.007 [12] 卢桂宾. 不同外源钙剂对壶瓶枣裂果率及品质的影响 [J]. 山西林业科技, 2016(3):37−39. doi: 10.3969/j.issn.1007-726X.2016.03.012LU G B. Effects of different exogenous calcium agent on fruit cacking rate and quality of huping jujube [J]. Shanxi Forestry Science and Technology, 2016(3): 37−39.(in Chinese) doi: 10.3969/j.issn.1007-726X.2016.03.012 [13] 张峰, 李养义, 张晓东, 等. 硅钙肥对库尔勒香梨果实品质的影响 [J]. 中国农学通报., 2020, 36(4):56−60.ZHANG F, LI Y Y, ZHANG X D, ett al. Effects of silicon and calcium fertilizer on fruit quality of korla fragrant pear [J]. Chinese Agricultural Science Bulletin, 2020, 36(4): 56−60.(in Chinese) [14] TAHJIB-UL-ARIF M, ROY P R, AL MAMUN SOHAG A, et al. Exogenous calcium supplementation improves salinity tolerance in BRRI Dhan28; a salt-susceptible high-yielding Oryza sativa cultivar [J]. Journal of Crop Science and Biotechnology, 2018, 21(4): 383−394. doi: 10.1007/s12892-018-0098-0 [15] 莫飞旭, 田茂科, 龙友华, 等. 叶面施钙对猕猴桃钙含量、品质及贮藏性的影响 [J]. 中国南方果树, 2019, 48(2):116−120. doi: 10.13938/j.issn.1007-1431.20180430MO F X, TIAN M K, LONG Y H, et al. Effect of foliar application of calcium on calcium content, quality and storage of kiwifruit [J]. South China Fruits, 2019, 48(2): 116−120.(in Chinese) doi: 10.13938/j.issn.1007-1431.20180430 [16] HE L, YU L, LI B, et al. The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress [J]. BMC Plant Biology, 2018, 18(1): 180. doi: 10.1186/s12870-018-1393-3 [17] 陈兴强. 不同钙肥对几种蔬菜幼苗质量的影响[D]. 广州: 华南农业大学, 2018.CHEN X Q. Effects of different calcium fertilizers on seedling quality of several vegetables[D]. Guangzhou: South China Agricultural University, 2018. [18] 周君, 肖伟, 陈修德, 等. 外源钙对‘黄金梨’叶片光合特性及果实品质的影响 [J]. 植物生理学报, 2018(3):449−455. doi: 10.13592/j.cnki.ppj.2017.0519ZHOU J, XIAO W, CHEN X D, et al. Effect of exogenous calcium on leaf photosynthetic characteristics and fruit quality of ‘Whangkeumbae' pear [J]. Plant Physiology Journal, 2018(3): 449−455.(in Chinese) doi: 10.13592/j.cnki.ppj.2017.0519 [19] 曹洪凤, 金丽华, 王忠钰, 等. 辣椒喷施中量元素(硅、钙)水溶肥料效果分析 [J]. 北京农业, 2016(2):62−63. doi: 10.3969/j.issn.1000-6966.2016.02.034CAO H F, JIN L H, WANG Z Y, et al. Analysis of the Effect of Pepper Spraying with Water-soluble Fertilizers with Moderate Elements (Silicon, Calcium) [J]. Beijing Agriculture, 2016(2): 62−63.(in Chinese) doi: 10.3969/j.issn.1000-6966.2016.02.034 [20] 王玉霞, 李芳东, 李延菊, 等. 不同类型叶面肥对晚熟油桃‘福美’果实品质的影响 [J]. 山东农业科学, 2018(9):48−50. doi: 10.14083/j.issn.1001-4942.2018.09.011WANG Y X, LI F D, LI Y J, et al. Effects of different kinds of foliar fertilizers on fruit quality of late ripening nectarine cultivar fumei [J]. Shandong Agricultural Sciences, 2018(9): 48−50.(in Chinese) doi: 10.14083/j.issn.1001-4942.2018.09.011 [21] 商振清. 钙对生物膜透性的影响机理 [J]. 河北农业大学学报, 1988, 11(1):113−117.SHANG Z Q. The possible mechanism of calcium affecting the permeability of biomembrane [J]. Journal of Agricultural University of Hebei, 1988, 11(1): 113−117.(in Chinese) [22] 周君, 肖伟, 陈修德, 等. 喷施氨基酸钙对黄金梨光合性能、果实钙含量及品质的影响 [J]. 山东农业大学学报(自然科学版), 2018(4):551−555,590.ZHOU J, XIAO W, CHEN X D, et al. Effect of spraying amino acid-Ca on photosynthetic characteristics, calcium content and quality of ‘Whangkeumbae’ Pear [J]. Journal of Shandong Agricultural University (Natural Science Edition), 2018(4): 551−555,590.(in Chinese) [23] 丁双双, 李燕婷, 袁亮, 等. 小分子有机物螯合钙肥对樱桃番茄产量、品质和养分吸收的影响 [J]. 中国土壤与肥料, 2015(5):61−66. doi: 10.11838/sfsc.20150511DING S S, LI Y T, YUAN L, et al. Effects of small molecular organics chelated calcium fertilizer on cherry tomato yield, quality and nutrients absorption [J]. Soil and Fertilizer Sciences in China, 2015(5): 61−66.(in Chinese) doi: 10.11838/sfsc.20150511 [24] 车玉红. 钙肥对红富士苹果果实品质及生理生化特性影响的研究[D]. 杨凌: 西北农林科技大学, 2005.CHE Y H. Study on the Effect of Calcium Fertilizations on Fruit Quality and Physiological and Biochemical Characteristics of Red Fuji Apple[D]. Yangling: Northwest A&F University, 2005. [25] 吴文强, 刘瑜, 李萍, 等. 糖醇螯合钙对茄子生长、产量和品质的影响 [J]. 中国蔬菜, 2013(24):46−48. doi: 10.3969/j.issn.1000-6346.2013.24.010WU W Q, LIU Y, LI P, et al. Effects of sugar alcohol complex calcium on eggplant growth, yield and quality [J]. China Vegetables, 2013(24): 46−48.(in Chinese) doi: 10.3969/j.issn.1000-6346.2013.24.010 [26] 关志华, 程智慧. 外源施钙对加工番茄果实硬度及品质相关指标的影响 [J]. 西北农林科技大学学报(自然科学版), 2009(10):145−150.GUAN Z H, CHENG Z H. Effects of foliage application of calcium on fruit firmness and quality related indexes in processing tomato [J]. Journal of Northwest A & F University (Natural Science Edition), 2009(10): 145−150.(in Chinese) [27] 付雅丽, 王献革, 索向敏, 等. 外源钙对‘金太阳杏’果实品质的影响 [J]. 中国园艺文摘, 2015, 31(12):30,115.FU Y L, WANG X G, SUO X M, et al. Effect of exogenous calcium on the quality of gold-Sun apricot fruits [J]. Chinese Horticulture Abstracts, 2015, 31(12): 30,115.(in Chinese) [28] 李敏, 厉恩茂, 李壮, 等. 氨基酸钙叶面微肥对苹果缺素症的矫正及果实品质的影响 [J]. 江苏农业科学, 2013(11):180−182. doi: 10.3969/j.issn.1002-1302.2013.11.068LI M, LI E M, LI Z, et al. Effects of amino acid calcium foliar micro-fertilizer on correction of apple deficiency and fruit quality [J]. Jiangsu Agricultural Sciences, 2013(11): 180−182.(in Chinese) doi: 10.3969/j.issn.1002-1302.2013.11.068 [29] 丁双双, 李燕婷, 袁亮, 等. 糖醇和氨基酸对小白菜钙营养及生长、品质的影响 [J]. 植物营养与肥料学报, 2016, 22(3):744−751. doi: 10.11674/zwyf.15093DING S S, LI Y T, YUAN L, et al. Effects of sugar alcohols and amino acids on growth, quality and calcium nutrition of Chinese cabbage [J]. Journal of Plant Nutrition and Fertilizer, 2016, 22(3): 744−751.(in Chinese) doi: 10.11674/zwyf.15093 [30] 李腾升, 魏倩倩, 黄明丽, 等. 糖醇螯合肥在农业上的应用研究进展 [J]. 土壤学报, 2021, 58(6):1393−1403. doi: 10.11766/trxb202005180245LI T S, WEI Q Q, HUANG M L, et al. Research progresses on the application of sugar alcohol chelated fertilizers in agriculture [J]. Acta Pedologica Sinica, 2021, 58(6): 1393−1403.(in Chinese) doi: 10.11766/trxb202005180245 [31] 罗安程, 周焱. 植物对氨基酸的吸收及其生理效应 [J]. 氨基酸杂志, 1993(1):22−24.LUO A C, ZHOU Y. The absorption of amino acids by plants and their effect on plant physiological process [J]. Amino Acids & Biotic Resources, 1993(1): 22−24.(in Chinese) [32] 王莹, 史振声, 王志斌, 等. 植物对氨基酸的吸收利用及氨基酸在农业中的应用 [J]. 中国土壤与肥料, 2008(1):6−11. doi: 10.3969/j.issn.1673-6257.2008.01.002WANG Y, SHI Z S, WANG Z B, et al. Absorption and utilization of amino acids by plant and application of amino acids on agiculture [J]. Soil and Fertilizer Sciences in China, 2008(1): 6−11.(in Chinese) doi: 10.3969/j.issn.1673-6257.2008.01.002 [33] 张梅, 刘曙光, 史向群, 等. 氨基酸及氨基酸螯合肥生物学功效研究进展 [J]. 湖北农业科学, 2019(S1):1−3,6.ZHANG M, LIU S G, SHI X Q, et al. Biological effect of plant amion acids and amino acids trace-element fertilizers [J]. Hubei Agricultural Sciences, 2019(S1): 1−3,6.(in Chinese)