低温和植物生长调节剂处理对菘蓝抽薹开花的影响

吴红; 李勇军; 丁银花; 韩大雷; 赵嘉豪; 陈诗曼

doi:10.19303/j.issn.1008-0384.2022.012.006

低温和植物生长调节剂处理对菘蓝抽薹开花的影响

doi: 10.19303/j.issn.1008-0384.2022.012.006

1.
江苏农牧科技职业学院园林园艺学院，江苏　泰州　225300
2.
民乐县诚泰药业有限公司，甘肃民乐　734502

基金项目: 江苏现代农业产业技术体系建设专项（JATS【2022】301）；江苏省大学生创新创业训练计划项目（202012806061H）；江苏农牧科技职业学院项目（NSF2021ZR18）

详细信息

作者简介:
吴红（1979−），女，硕士，副教授，研究方向：园艺植物遗传育种与繁育（E-mail：wh02gs@126.com）

通讯作者:
李勇军（1975−），男，博士，教授，研究方向：中药学（E-mail：yf6011@hotmail.com）

中图分类号: R 282.2
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- 被引次数: 0
出版历程
- 收稿日期: 2022-08-08
- 修回日期: 2022-10-22
- 网络出版日期: 2023-03-05
- 刊出日期: 2022-03-28

Effects of Low Temperature and Plant Growth Regulator on Bolting and Flowering of Isatis indigotica

1.
Landscape and Horticulture College, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu　225300, China
2.
Minle Chengtai Pharmaceutical Co., Ltd., Minle, Gansu　734502, China

摘要

摘要: 目的探讨温度和植物生长调节剂对菘蓝生长以及抽薹开花的影响，为进一步阐明菘蓝抽薹开花的调控机制提供依据。方法以中青一号菘蓝种子为试验材料，在人工气候箱中对菘蓝幼苗进行2、4、6 ℃低温春化处理，研究低温春化对菘蓝抽薹性状的影响，同时在25 ℃和4 ℃条件下测定菘蓝茎尖内源激素的变化以及赤霉素和烯效唑对菘蓝抽薹率的影响。结果（1）低温处理可促进菘蓝抽薹、开花和花芽分化，4 ℃春化处理40 d的幼苗在定植后27 d， 12片叶时开始开花，比2 ℃处理和6 ℃处理的植株平均提前了4个节位；（2）茎尖内源激素含量分析表明，低温处理增加菘蓝茎尖中3-吲哚乙酸（IAA）、赤霉素（GA）和脱落酸（ABA）的含量，低温和25 ℃处理下菘蓝IAA含量和GA缓慢上升后下降，IAA含量在出苗后40 d达到最高值，GA含量出苗后50 d左右，且抽薹后IAA和GA含量低于抽薹前，ABA含量在整个生长季节持续增加；（3）低温和GA协同处理的植株表现出加性正效应，低温加速了菘蓝的抽薹、开花进程，这种促进不仅通过在茎尖诱导产生GA，还通过低温与激素之间的相互作用；烯效唑处理显著降低了菘蓝的抽薹率，而GA则可以打破这种抑制。结论低温春化促进菘蓝抽薹和开花，低温处理提高了GA、IAA和ABA的含量，外源GA处理可代替低温处理诱导植物在缺乏低温的条件下开花，而GA合成抑制剂烯效唑的作用相反。
- 菘蓝 /
- 低温 /
- 植物生长调节剂 /
- 抽薹 /
- 开花
Abstract: Objective Effects of low temperature and plant growth regulator on the growth, bolting, and flowering of Isatis indigotica plants were investigated. Method Seeds of Isatis. indigotica Zhongqing No. 1 were exposed to 2, 4, or 6 ℃ in a climate-controlled chamber for vernalization to observe the bolting and flowering of the emerged seedlings. Endogenous hormones in the shoot apex and effects of gibberellin and uniconazole on the bolting rate of the plants under 25 ℃ and 4 ℃ were determined. Result (1) Low temperature vernalization promoted the process of bolting, flowering, and bud differentiation of the plants. The seedlings emerged from a 40 d/4 ℃ vernalization began to flower at 27 d after planting ,which had 12 leaves on a plant. and It's 4 nodes earlier than that of 2 ℃ or 6 ℃ vernalization. (2) The low temperature treatment increased the contents of IAA, GA, and ABA in the seedling stem tips. and under 4 ℃ and 25 ℃ treatment, the IAA and GA content slowly increased and followed by a decline ,IAA peaked 40 d after germinating, while GA 50 d, and the IAA and GA content was lower than that before bolting, while ABA continued to rise throughout the growing season. (3) The combined low temperature and GA treatment showed additive positive effect, and which accelerated the plant bolting and flowering, not only due to the GA induction at the stem tip but also the interaction between low temperature and hormone. Uniconazole treatment significantly reduced the bolting rate of Isatis indigotica, while GA could break this inhibition Conclusion Low temperature vernalization promotes bolting and flowering of Isatis indigotica. It promoted the GA, IAA, and ABA generation in the seedlings. Exogenous GA could induce flowering of the plants without a low temperature treatment, but uniconazole would retard bolting.
- Isatis indigotica /
- low temperature /
- plant growth regulator /
- bolting /
- flowering

HTML全文

图 1 低温春化处理对菘蓝现蕾、抽薹及开花时间的影响

Figure 1. Effect of low temperature vernalization on budding, bolting, and flowering time of I. indigotica

下载: 全尺寸图片幻灯片

图 2 低温春化处理对菘蓝花芽分化节位（叶片数）的影响

用LSD法作效应分析。不同小写字母表示不同温度处理差异显著（ P＜0.05），不同大写字母表示不同温度处理差异极显著（ P＜0.01），下同。

Figure 2. Effect of low temperature vernalization on node position (leaf number) of bud differentiation of I. indigotica flowers

LSD method was used for significant analysis. The lowercase letters indicate significant difference (P＜0.05), uppercase letters indicate extremely significant difference (P＜0.01). The same below.

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图 3 低温春化处理对菘蓝株高和茎粗的影响

Figure 3. Effect of low temperature vernalization on plant height and stem diameter of I. indigotica

下载: 全尺寸图片幻灯片

图 4 低温春化处理对菘蓝内源激素含量的影响

Figure 4. Effect of low temperature vernalization on hormone contents of I. indigotica

下载: 全尺寸图片幻灯片

表 1 低温和植物生长调节剂处理对菘蓝抽薹率的影响

Table 1. Effects of low temperature and plant growth regulator on bolting rate of I. indigotica

处理天数 Treatment days/d	25 ℃				4 ℃
处理天数 Treatment days/d	T1	T2	T3	T4	T1	T2	T3	T4
10	0.00	0.00	0.00	0.00	0.00	0.00	10.95	0.00
13	3.44	0.00	8.65	3.69f	15.39	4.43	24.02	13.23
16	6.86	0.00	14.21	8.33	22.15	6.97	37.82	30.31
19	9.19	0.00	24.43	16.18	28.15	14.24	74.12	52.22
22	12.66	5.65	32.82	21.56	30.78	14.65	93.08	57.63
25	12.89	6.09	37.07	22.84	37.90	19.83	98.59	59.53
28	15.74	9.00	43.81	30.27	65.04	18.82	100.15	79.38
31	16.58	9.00	54.01	44.11	64.82	23.33	100.63	79.85
34	21.53	10.65	64.01	43.19	80.07	24.52	100.49	92.89

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表 2 方差分析

Table 2. Variance analysis

方差来源 Source of variance	平方和 Sum of squares	自由度 Freedom	均方 Mean square	F值 F value	显著性 Significance
温度 Temperature	34167.953	1	34167.953	1970.994	**
生长调节剂 Growth regulator	44986.574	3	14995.525	865.024	**
处理天数 Treatment days	58640.226	8	7330.028	422.836	**
温度×生长调节剂 Temperature × growth regulator	5546.245	3	1848.748	106.646	**
温度×处理天数 Temperature × treatment days	7748.251	8	968.531	55.87	**
生长调节剂×处理天数 Growth regulator× treatment days	13041.667	24	543.403	31.346	**
温度×生长调节剂×处理天数 Temperature × growth regulator× treatment days	2931.996	24	122.166	7.047	**
误差 Error	2496.296	144	17.335
R² =0.978，“”表示在0.01水平上具有极显著差异（双侧）。 R² =0.978; represents significant correlation at P＜0.01 (bilateral).

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参考文献(37)

[1]	AMASINO R M, MICHAELS S D. The timing of flowering [J]. Plant Physiology, 2010, 154(2): 516−520. doi: 10.1104/pp.110.161653
[2]	DAHANAYAKE S R, GALWEY N W. Effects of interactions between low-temperature treatments, gibberellin (GA 3) and photoperiod on flowering and stem height of spring rape (Brassica napus var. annua) [J]. Annals of Botany, 1999, 84(3): 321−327. doi: 10.1006/anbo.1999.0920
[3]	杨峰, 李晓梅, 李跃建, 等. 低温处理及光周期诱导不同抽薹性萝卜抽薹开花的研究 [J]. 长江蔬菜, 2018(4):55−58. YANG F, LI X M, LI Y J, et al. Effects of low temperature treatment and photoperiod inducing on bolting and flowering of different radish [J]. Journal of Changjiang Vegetables, 2018(4): 55−58.(in Chinese)
[4]	秦成萌, 杨延杰, 林多. 植物生长调节剂对不同萝卜品种抽薹效应的影响 [J]. 吉林农业科学, 2013, 38(5):77−78,85. doi: 10.16423/j.cnki.1003-8701.2013.05.028 QIN C M, YANG Y J, LIN D. Effects of plant growth regulators on bolting of different radish varieties [J]. Journal of Jilin Agricultural Sciences, 2013, 38(5): 77−78,85.(in Chinese) doi: 10.16423/j.cnki.1003-8701.2013.05.028
[5]	汪精磊, 李锡香, 邱杨, 等. 十字花科蔬菜抽薹开花性状的调控机理和分子育种研究进展 [J]. 植物遗传资源学报, 2015, 16(6):1283−1289. doi: 10.13430/j.cnki.jpgr.2015.06.021 WANG J L, LI X X, QIU Y, et al. Research advances on molecular markers and regulation mechanism for the bolting and blooming characteristics of Brassicaceae vegetables [J]. Journal of Plant Genetic Resources, 2015, 16(6): 1283−1289.(in Chinese) doi: 10.13430/j.cnki.jpgr.2015.06.021
[6]	杨金兰, 刘艳波, 张舜. 萝卜生产中抽薹的原因及其预防措施 [J]. 北方园艺, 2015(23):71−74. doi: 10.11937/bfyy.201523021 YANG J L, LIU Y B, ZHANG S. Causes and preventive measures of bolting in radish production [J]. Northern Horticulture, 2015(23): 71−74.(in Chinese) doi: 10.11937/bfyy.201523021
[7]	王超, 张韬, 吴世昌. 春结球甘蓝未熟先期抽薹原因及防止对策 [J]. 北方园艺, 2001(4):48. doi: 10.3969/j.issn.1001-0009.2001.04.012 WANG C, ZHANG T, WU S C. Causes and prevention measures of premature bolting of spring cabbage [J]. Northern Horticulture, 2001(4): 48.(in Chinese) doi: 10.3969/j.issn.1001-0009.2001.04.012
[8]	宋贤勇, 柳李旺, 龚义勤, 等. 春萝卜抽薹过程中内源激素含量变化分析 [J]. 植物研究, 2007, 27(2):182−185. doi: 10.3969/j.issn.1673-5102.2007.02.014 SONG X Y, LIU L W, GONG Y Q, et al. Hormone content and balance during bolting of spring radish (Raphanus sativus L. ) [J]. Bulletin of Botanical Research, 2007, 27(2): 182−185.(in Chinese) doi: 10.3969/j.issn.1673-5102.2007.02.014
[9]	何磊, 姚南, 裴芸, 等. 萝卜抽薹进程中生理生化变化及开花基因时空表达比较 [J]. 分子植物育种, 2020, 18(20):6837−6843. doi: 10.13271/j.mpb.018.006837 HE L, YAO N, PEI Y, et al. Physiological and biochemical changes and spatial and temporal expressions of flowering genes in radish(Raphanus sativus L. ) during bolting [J]. Molecular Plant Breeding, 2020, 18(20): 6837−6843.(in Chinese) doi: 10.13271/j.mpb.018.006837
[10]	王凤华, 李光远, 李盛, 等. 低温春化对樱桃萝卜种子萌发及成花的影响 [J]. 北方园艺, 2016(5):39−42. doi: 10.11937/bfyy.201605011 WANG F H, LI G Y, LI S, et al. Influence of vernalization on germination and flowering of cherry radish [J]. Northern Horticulture, 2016(5): 39−42.(in Chinese) doi: 10.11937/bfyy.201605011
[11]	宋明, 汤青林, 王志敏, 等. 低温春化和GA3处理后芥菜发育的生化变化 [J]. 西南大学学报(自然科学版), 2009, 31(12):48−52. SONG M, TANG Q L, WANG Z M, et al. Study on physiological and biochemical changes after low-temperature vernalization and GA3 treatments in mustard [J]. Journal of Southwest University (Natural Science Edition), 2009, 31(12): 48−52.(in Chinese)
[12]	黎黎, 刘娟, 阳梦瑶, 等. 低温春化与光周期调控对普通白菜抽薹性状的影响 [J]. 中国瓜菜, 2021, 34(5):45−51. doi: 10.3969/j.issn.1673-2871.2021.05.009 LI L, LIU J, YANG M Y, et al. Effects of low temperature vernalization and photoperiod regulation on bolting characters of pakchoi [J]. China Cucurbits and Vegetables, 2021, 34(5): 45−51.(in Chinese) doi: 10.3969/j.issn.1673-2871.2021.05.009
[13]	刘倩倩, 王康才, 王乾, 等. 低温春化对菘蓝体内碳氮代谢的影响 [J]. 中国中药杂志, 2013, 38(10):1484−1488. LIU Q Q, WANG K C, WANG Q, et al. Effect of low-temperature vernalization on metabolism of carbon and nitrogen of Isatis indigotica [J]. China Journal of Chinese Materia Medica, 2013, 38(10): 1484−1488.(in Chinese)
[14]	陈奇, 袁金海, 孙万仓, 等. 低温胁迫下白菜型冬油菜与春油菜叶片光合特性及内源激素变化比较 [J]. 中国油料作物学报, 2017, 39(1):37−46. doi: 10.7505/j.issn.1007-9084.2017.01.006 CHEN Q, YUAN J H, SUN W C, et al. Leaf photosynthetics and endogenous hormones of spring and winter Rapa(Brassica rapa L. ) under low temperature [J]. Chinese Journal of Oil Crop Sciences, 2017, 39(1): 37−46.(in Chinese) doi: 10.7505/j.issn.1007-9084.2017.01.006
[15]	杨永岗, 张化生, 李亚莉, 等. 高原夏季胡萝卜肉质根内源激素变化及其与先期抽薹的关系 [J]. 园艺学报, 2010, 37(7):1102−1108. doi: 10.16420/j.issn.0513-353x.2010.07.010 YANG Y G, ZHANG H S, LI Y L, et al. Endogenous hormone content of fleshy root in relation to early bolting in summer cultivated carrot on plateau [J]. Acta Horticulturae Sinica, 2010, 37(7): 1102−1108.(in Chinese) doi: 10.16420/j.issn.0513-353x.2010.07.010
[16]	马学才, 方彦, 刘丽君, 等. 低温胁迫下不同温敏性油菜保护酶活性及内源激素变化 [J]. 甘肃农业大学学报, 2021, 56(3):86−94. doi: 10.13432/j.cnki.jgsau.2021.03.012 MA X C, FANG Y, LIU L J, et al. Change of protective enzyme activities and endogenous hormones in different temperature-sensitive rapes under low-temperature stress [J]. Journal of Gansu Agricultural University, 2021, 56(3): 86−94.(in Chinese) doi: 10.13432/j.cnki.jgsau.2021.03.012
[17]	蒋宇霞, 甘立军, 夏凯. IAA和GA3在调控豌豆黄化苗茎切段伸长生长中的相互作用 [J]. 植物生理学通讯, 2006, 42(2):207−212. JIANG Y X, GAN L J, XIA K. Interactions between IAA and GA3 in regulation of etiolated stem segment elongation in Pisum sativum L [J]. Plant Physiology Communications, 2006, 42(2): 207−212.(in Chinese)
[18]	刘梦芸, 蒙美莲, 门福义, 等. GA3. IAA. CTK和ABA对马铃薯块茎形成调控作用的研究 [J]. 内蒙古农牧学院学报, 1997, 18(2):16−20. LIU M Y, MENG M L, MEN F Y, et al. Studies on the control of ga₃, zaa, ctk and Aba on the tuberization of potato [J]. Journal of Inner Mongolia Institute of Agriculture and Animal Husbandry, 1997, 18(2): 16−20.(in Chinese)
[19]	陈红霞, 王康才, 吕建军. 菘蓝春化机理的初步研究 [J]. 现代中药研究与实践, 2003, 17(5):6−8. doi: 10.13728/j.1673-6427.2003.05.002 CHEN H X, WANG K C, LU J J. Primary study on the mechanism of the vernalization of Isatis indigogitica [J]. Research and Practice of Chinese Medicines, 2003, 17(5): 6−8.(in Chinese) doi: 10.13728/j.1673-6427.2003.05.002
[20]	王淑芬, 徐文玲, 何启伟, 刘继保, 张传坤. 春化深度对萝卜抽薹的影响及抽薹过程中GA3和IAA含量的变化 [J]. 山东农业科学, 2003, 35(6):20−21. doi: 10.3969/j.issn.1001-4942.2003.06.009 WANG S F, XU W L, HE Q W, et al. Effects of vernalization depth on bolting of radish and changes of GA3 and IAA contents during bolting [J]. Shandong Agricultural Sciences, 2003, 35(6): 20−21.(in Chinese) doi: 10.3969/j.issn.1001-4942.2003.06.009
[21]	李盛. 春化处理对萝卜抽薹及开花的影响[D]. 洛阳: 河南科技大学, 2014. LI S. Influences of vernalization on bolting and blossom of radish[D]. Luoyang: Henan University of Science and Technology, 2014. (in Chinese)
[22]	刘莎莎. 光周期及低温处理对菠菜抽薹的影响[D]. 山东农业大学, 2010. LI S S. Effects of temperature and photoperiod on spinach bolting[D]. Taian: Shandong Agricultural University, 2010. (in Chinese)
[23]	ZANEWICH K P, ROOD S B. Vernalization and gibberellin physiology of winter canola (endogenous gibberellin (GA) content and metabolism of[3H]GA1 and[3H]GA20 [J]. Plant Physiology, 1995, 108(2): 615−621. doi: 10.1104/pp.108.2.615
[24]	孙日飞, 张淑江, 司家钢, 钮心恪, 吴飞燕. 春化和赤霉素对大白菜抽薹开花的影响 [J]. 中国蔬菜, 1999(3):14−17. doi: 10.3969/j.issn.1000-6346.1999.03.005 SUN R F, ZHANG S J, SI J G, et al. Effect of vernalization and gibberellin of growth and flowering time in heading Chinese cabbage [J]. China Vegetables, 1999(3): 14−17.(in Chinese) doi: 10.3969/j.issn.1000-6346.1999.03.005
[25]	OLSZEWSKI N, SUN T P, GUBLER F. Gibberellin signaling: Biosynthesis, catabolism, and response pathways [J]. The Plant Cell, 2002, 14(Suppl): S61−S80.
[26]	SUGE H, RAPPAPORT L. Role of gibberellins in stem elongation and flowering in radish [J]. Plant Physiology, 1968, 43(8): 1208−1214. doi: 10.1104/pp.43.8.1208
[27]	胡存彪, 陈云飞, 刘圣会, 等. 多效唑和赤霉素对结球白菜抽薹开花的调控 [J]. 山地农业生物学报, 2016, 35(2):73−75. doi: 10.15958/j.cnki.sdnyswxb.2016.02.014 HU C B, CHEN Y F, LIU S H, et al. Regulation of flowering time of Chinese cabbage by paclobutrazol and gibberellin [J]. Journal of Mountain Agriculture and Biology, 2016, 35(2): 73−75.(in Chinese) doi: 10.15958/j.cnki.sdnyswxb.2016.02.014
[28]	田皓, 王昱卜, 杜巍, 等. 外源生长素对叶用莴苣抽薹及相关生理的影响 [J]. 北京农学院学报, 2021, 36(4):20−24. doi: 10.13473/j.cnki.issn.1002-3186.2021.0404 TIAN H, WANG Y B, DU W, et al. Effects of exogenous auxin on bolting and related physiology of lettuce [J]. Journal of Beijing University of Agriculture, 2021, 36(4): 20−24.(in Chinese) doi: 10.13473/j.cnki.issn.1002-3186.2021.0404
[29]	黄珊. 当归早期抽薹分化和内源性激素变化研究[D]. 杨凌: 西北农林科技大学, 2020. HUANG S. Study on early bolting differentiation and endogenous hormone changes of Angelica sinensis[D]. Yangling: Northwest A & F University, 2020. (in Chinese)
[30]	朱玉野, 董丽华, 朱继孝, 等. 防风抽薹期内源激素的动态分析 [J]. 中草药, 2014, 45(13):1924−1927. doi: 10.7501/j.issn.0253-2670.2014.13.022 ZHU Y Y, DONG L H, ZHU J X, et al. Dynamic analysis on endo phylohormone during bolting time of roots in Saponikovia cata [J]. Chinese Traditional and Herbal Drugs, 2014, 45(13): 1924−1927.(in Chinese) doi: 10.7501/j.issn.0253-2670.2014.13.022
[31]	REGNAULT T, DAVIÈRE J M, HEINTZ D, et al. The gibberellin biosynthetic genes AtKAO1 and AtKAO2 have overlapping roles throughout Arabidopsis development [J]. The Plant Journal:for Cell and Molecular Biology, 2014, 80(3): 462−474. doi: 10.1111/tpj.12648
[32]	孙会军, 雷家军. 赤霉素对君子兰花期调控的研究 [J]. 北方园艺, 2008(4):172−174. SUN H J, LEI J J. Study on control of flowering with gibberellin treatment in Clivia miniata regel [J]. Northern Horticulture, 2008(4): 172−174.(in Chinese)
[33]	戴忠良, 潘跃平, 肖燕, 等. 不同浓度赤霉素处理对结球甘蓝抽薹和开花的影响 [J]. 上海农业学报, 2010, 26(4):69−71. doi: 10.3969/j.issn.1000-3924.2010.04.016 DAI Z L, PAN Y P, XIAO Y, et al. Effects of gibberellin treatments at different concentrations on bolting and flowering of common head cabbage [J]. Acta Agriculturae Shanghai, 2010, 26(4): 69−71.(in Chinese) doi: 10.3969/j.issn.1000-3924.2010.04.016
[34]	孟宪敏, 崔青青, 段韫丹, 等. 烯效唑对番茄幼苗嫁接愈合的促进作用及其机理研究 [J]. 园艺学报, 2022, 49(6):1275−1289. MENG X M, CUI Q Q, DUAN Y D, et al. Promoting effects of uniconazole on grafting formation of tomato seedlings and underlying mechanisms [J]. Acta Horticulturae Sinica, 2022, 49(6): 1275−1289.(in Chinese)
[35]	康云艳, 黄爱政, 杨暹, 等. 脱落酸和烯效唑对菜心抽薹特性和生长的影响 [J]. 广东农业科学, 2010, 37(12):49−51. doi: 10.3969/j.issn.1004-874X.2010.12.018 KANG Y Y, HUANG A Z, YANG X, et al. Effects of ABA and uniconazole on bolting characteristic and growth of flowering Chinese cabbage [J]. Guangdong Agricultural Sciences, 2010, 37(12): 49−51.(in Chinese) doi: 10.3969/j.issn.1004-874X.2010.12.018
[36]	王振龙. S3307、CCC浸种处理对无土栽培宇椒1号生长发育的影响 [J]. 辽宁农业职业技术学院学报, 2008, 10(2):7−8. doi: 10.3969/j.issn.1671-0517.2008.02.004 WANG Z L. Effects of soaking seeds with S3307 and CCC on the growth and development of soilless culture Yujiao No. 1 [J]. Journal of Liaoning Agricultural College, 2008, 10(2): 7−8.(in Chinese) doi: 10.3969/j.issn.1671-0517.2008.02.004
[37]	李宁毅, 刘冰. 植物生长延缓剂S₃₃₀₇对百合生长发育和内源激素的影响及相关性分析 [J]. 西北农业学报, 2010, 19(5):153−156. doi: 10.3969/j.issn.1004-1389.2010.05.034 LI N Y, LIU B. Effects of plant growth retardant S₃₃₀₇ on the growth and endogenous hormones contents of lily and the correlation analysis [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2010, 19(5): 153−156.(in Chinese) doi: 10.3969/j.issn.1004-1389.2010.05.034