Biological Characteristics of Geotrichum candidum–Pathogen of Sour Rot Disease on Pseudostellaria heterophylla
-
摘要:
目的 明确太子参酸腐病病原菌白地霉（Geotrichum candidum）FJAT-32652的生物学特性。 方法 测定白地霉在不同温度、pH值、光照、碳源和氮源培养条件下的菌落直径，以及孢子的致死温度和时长，并用显微镜观察孢子在不同培养时间的萌发情况。 结果 该病原菌生长最适温度为30 ℃，pH值为6，碳源为麦芽糖、乳糖和葡萄糖，氮源为硫酸铵，不同光照条件对 菌丝生长无显著影响，孢子的致死温度及时长为65 ℃水浴10 min。病原菌孢子培养3 h开始萌发，9 h形成菌丝，繁殖方式为裂殖。 结论 明确了太子参酸腐病病原菌白地霉FJAT-32652的生物学特性，为太子参酸腐病的后续研究提供基础数据。 Abstract:Objective Biological characteristics of the pathogen causing sour rot disease on Pseudostellaria heterophylla were studied. Method Diameters of Geotrichum candidum FJAT-32652 colonies on culture medium under varied temperature, pH, light, and carbon and nitrogen sources were measured to determine the optimal growth conditions for the pathogen. Lethal temperature and time of the spores were determined, and spore germination observed under a microscope. Results The optimum growth conditions for the pathogen included 30 ℃, pH 6, maltose, lactose, and glucose for carbon source, and ammonium sulfate for nitrogen source. Light exposure had no significant effect on the mycelial growth. Lethality occurred by submerging the spores in 65 ℃ hot water for 10 min. Spore germination started 3 h after incubation, and mycelia became visible in 9 h. Schizogamy was the reproduction mode of the pathogen. Conclusion The biological characteristics of G. candidum were determined for subsequent studies on the sour rot disease of P. heterophylla. -
-
[1] 宋叶, 林东, 梅全喜, 等. 太子参化学成分及药理作用研究进展 [J]. 中国药师, 2019, 22(8):1506−1510.SONG Y, LIN D, MEI Q X, et al Study advances in chemical constituents and pharmacological activities of Radix pseudostellariae [J]. China Pharmacist, 2019, 22(8): 1506−1510.(in Chinese) [2] 傅兴圣, 刘训红, 许虎, 等. 太子参研究现状与研发趋势 [J]. 中国新药杂志, 2012, 21(7):757−760.FU X S, LIU X H, XU H, et al. Research status and trends of pseudostellariae Radix [J]. Chinese Journal of New Drugs, 2012, 21(7): 757−760.(in Chinese) [3] 陈燕萍, 陈梅春, 刘欣, 等. 太子参新病害酸腐病的病原菌鉴定 [J]. 植物病理学报, 2021, 51(3):464−468.CHEN Y P, CHEN M C, LIU X, et al. Identification of the pathogen causing a new sour-rot disease on the Pseudostellaria heterophylla [J]. Acta Phytopathologica Sinica, 2021, 51(3): 464−468.(in Chinese) [4] 黄国林, 刘月廉, 朱艳秋. 荔枝酸腐病病原菌的形态学及分子生物学鉴定 [J]. 黑龙江农业科学, 2021(2):66−69.HUANG G L, LIU Y L, ZHU Y Q. Morphological and molecular identification of the pathogen of Litchi sour rot [J]. Heilongjiang Agricultural Sciences, 2021(2): 66−69.(in Chinese) [5] THORNTON C R, SLAUGHTER D C, DAVIS R M. Detection of the sour-rot pathogen Geotrichum candidum in tomato fruit and juice by using a highly specific monoclonal antibody-based ELISA [J]. International Journal of Food Microbiology, 2010, 143(3): 166−172. doi: 10.1016/j.ijfoodmicro.2010.08.012 [6] HORITA H, HATTA Y. Sour rot of carrot caused by Geotrichum candidum in Japan [J]. Journal of General Plant Pathology, 2016, 82(1): 65−68. doi: 10.1007/s10327-015-0638-3 [7] 高莹, 魏少刚, 谈静惠, 等. 欧李酸腐病病原菌鉴定及其生物学特性研究 [J]. 山西农业大学学报(自然科学版), 2012, 32(4):328−332.GAO Y, WEI S G, TAN J H, et al. Identification and biological characteristics of the pathogen causing sour rot of gerasus humilis [J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2012, 32(4): 328−332.(in Chinese) [8] 杨晓, 龚林忠, 张瑛, 等. 4省(区)葡萄果实病害发生与危害调查 [J]. 中国南方果树, 2021, 50(1):106−109,114.YANG X, GONG L Z, ZHANG Y, et al. Investigation on occurrence and damage of grape fruit diseases in 4 provinces (regions) [J]. South China Fruits, 2021, 50(1): 106−109,114.(in Chinese) [9] 冷飞凡, 姜倩, 曾慧, 等. 脐橙酸腐菌分离鉴定及抑菌研究 [J]. 食品科技, 2017, 42(9):8−12.LENG F F, JIANG Q, ZENG H, et al. Identification and antifungal activity research of navel orange Geotrichurn candidum [J]. Food Science and Technology, 2017, 42(9): 8−12.(in Chinese) [10] 刘寒寒, 杨书珍, 李哲, 等. 碳酸铵对柑橘酸腐病菌的抑制效果及作用机制 [J]. 食品科学, 2021, 42(3):204−210.LIU H H, YANG S Z, LI Z, et al. Inhibitory effect and action mechanism of ammonium carbonate against sour rot in Citrus fruit [J]. Food Science, 2021, 42(3): 204−210.(in Chinese) [11] 赵荣华, 陈光, 白世践, 等. 几种化学药剂对吐鲁番无核白葡萄白粉病和酸腐病的防治效果 [J]. 安徽农业科学, 2014, 42(19):6245−6246.ZHAO R H, CHEN G, BAI S J, et al. Control effects of several chemicals against powdery mildew and sour rot of Turpan raisin grape [J]. Journal of Anhui Agricultural Sciences, 2014, 42(19): 6245−6246.(in Chinese) [12] 开凯, 毕婉玲, 张卫, 等. 番茄酸腐病病原菌分离鉴定和抑菌剂抑制效果 [J]. 合肥工业大学学报(自然科学版), 2021, 44(7):987−992.KAI K, BI W L, ZHANG W, et al. Isolation and identification of tomato sour rot pathogens and control effect of bacteriostatic agents [J]. Journal of Hefei University of Technology (Natural Science), 2021, 44(7): 987−992.(in Chinese) [13] 赵一洁, 唐毅, 王威浩, 等. 蜜橘酸腐病病原菌的分离鉴定以及不同抑菌剂处理对其控制效果 [J]. 食品科学, 2017, 38(7):230−237.ZHAO Y J, TANG Y, WANG W H, et al. Isolation and identification of sour rot pathogen of Satsuma mandarin and inhibitory effects of three antifungal substances on it [J]. Food Science, 2017, 38(7): 230−237.(in Chinese) [14] 张夏兰, 李兴红, 蔡建波, 等. 部分葡萄品种酸腐病田间发病情况调查 [J]. 植物保护, 2012, 38(6):119−122.ZHANG X L, LI X H, CAI J B, et al. A field survey of grape sour rot on some grape cultivars [J]. Plant Protection, 2012, 38(6): 119−122.(in Chinese) [15] 蔡建波, 李兴红, 孔繁芳, 等. 蓬莱地区酿酒葡萄果实病害调查及室内抗病性测定 [J]. 中国农学通报, 2010, 26(9):311−314.CAI J B, LI X H, KONG F F, et al. A survey of fruit-rotting disease and resistance of wine grape cultivars in Penglai [J]. Chinese Agricultural Science Bulletin, 2010, 26(9): 311−314.(in Chinese) [16] 苟攀宁, 薛应钰, 陈军宏, 等. 油桃酸腐病病菌的分离鉴定及其生物学特性 [J]. 甘肃农业大学学报, 2021(6):97−103.GOU P N, XUE Y Y, CHEN J H, et al. Isolation and identification of nectarine acid rot disease and its biological characteristics [J]. Journal of Gansu Agricultural University, 2021(6): 97−103.(in Chinese) [17] 李路, 李蔚, 车金鑫, 等. p-茴香醛抑制柑橘酸腐病菌的作用机制 [J]. 食品科学, 2020, 41(9):133−138.LI L, LI W, CHE J X, et al. Antifungal activity and mechanism of p-anisaldehyde aganist Geotrichum citri-aurantii [J]. Food Science, 2020, 41(9): 133−138.(in Chinese) [18] 李登勇, 王建国, 支胡钰, 等. 柑橘酸腐病拮抗菌的筛选及其对柑橘品质的影响 [J]. 安徽农业科学, 2019, 47(8):186−191.LI D Y, WANG J G, ZHI H Y, et al. Screening of antagonistic Bacillus of Citrus sour rot and its effects on the quality of Citrus [J]. Journal of Anhui Agricultural Sciences, 2019, 47(8): 186−191.(in Chinese) [19] 王丽, 赵盼, 孟祥红. 壳聚(寡)糖对柑橘酸腐、黑腐病菌的抑制作用及对采后病害的防治 [J]. 食品工业科技, 2011, 32(12):424−428.WANG L, ZHAO P, MENG X H. Antifungal activity of chitosan(oligochitosan) on Geotrichum candidum and Alternaria citri of Citrus and postharvest disease control [J]. Science and Technology of Food Industry, 2011, 32(12): 424−428.(in Chinese) [20] 杨蕊, 杨峰, 赵瑞丽, 等. 番茄酸腐病菌的生物学特性研究 [J]. 河南农业科学, 2013, 42(8):71−74.YANG R, YANG F, ZHAO R L, et al. Biological characteristics of Geotrichum candidum from tomato [J]. Journal of Henan Agricultural Sciences, 2013, 42(8): 71−74.(in Chinese) [21] THOMIDIS T, PRODROMOU I, FARMAKIS A, et al. Effect of temperature on the growth of Geotrichum candidum and chemical control of sour rot on tomatoes [J]. Tropical Plant Pathology, 2021, 46(5): 545−552. doi: 10.1007/s40858-021-00453-1 [22] 蔡学清, 林通, 谢玲平, 等. 福建荔枝酸腐菌的生物学特性研究 [J]. 热带作物学报, 2009, 30(12):1858−1864.CAI X Q, LIN T, XIE L P, et al. Biological characteristics of Geotrichum candidum from lychee in Fujian [J]. Chinese Journal of Tropical Crops, 2009, 30(12): 1858−1864.(in Chinese) [23] SHIMIZU K, HOSOI J, OBARA K, et al. Electron microscopic study of negatively stained ribosomal subunits from Geotrichum candidum [J]. Journal of Electron Microscopy, 1978, 27(2): 111−117. [24] CHENG H, TANG W, WANG H Y, et al. First report of Geotrichum candidum causing postharvest sour rot on kiwifruits in China [J]. Plant disease, 2020, 105(5): 1. [25] ZHANG L, LI Y H, ZHANG X, et al. First Report of Sour Rot Caused by Geotrichum candidum on Mori fructus in China [J]. Plant Disease, 2018, 102(12): 2640−2641. -
下载:
点击查看大图
图(7)
计量
- 文章访问数: 681
- HTML全文浏览量: 283
- PDF下载量: 71
- 被引次数: 0
