外源褪黑素对青椒幼苗耐冷性影响

练冬梅; 赖正锋; 李洲; 林碧珍; 姚运法; 张少平; 洪建基

doi:10.19303/j.issn.1008-0384.2022.010.006

外源褪黑素对青椒幼苗耐冷性影响

doi: 10.19303/j.issn.1008-0384.2022.010.006

福建省农业科学院亚热带农业研究所，福建　漳州 363005

基金项目: 福建省科技计划公益类专项（2022R1030002、2021R1030007）；福建省财政专项—福建省农业科学院科技创新团队建设项目（CXTD2021006-3）

详细信息

作者简介:
练冬梅（1987−），女，硕士，助理研究员，主要从事特色蔬菜育种与栽培研究（E-mail：woshildm1987@163.com）

通讯作者:
洪建基（1967−），男，研究员，主要从事特色作物育种与综合利用研究（E-mail：hjianji@163.com）

中图分类号: S 641.3
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出版历程
- 收稿日期: 2022-08-05
- 修回日期: 2022-09-03
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-10-30

Effects of Exogenous Melatonin on Cold Resistance of Green Pepper Seedlings

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian　363005, China

摘要

摘要: 目的明确外源褪黑素（Melatonin，MT）对提高青椒幼苗耐冷性的生理机制和适宜喷施浓度。方法以布兰妮青椒幼苗为试验材料，采用基质盆栽种植，研究全株喷施不同浓度外源MT（0、25、50、75、100 µmol·L⁻¹）对4 ℃低温胁迫下青椒幼苗生长、抗氧化酶活性、渗透调节物质、光合特性的影响。结果与4 ℃低温胁迫不喷施外源MT相比（CK），喷施外源MT能增强青椒幼苗叶片过氧化氢酶（CAT）活性、超氧化物歧化酶（SOD）活性、脯氨酸（Pro）含量，降低叶片丙二醛（MDA）含量、相对电导率和胞间CO₂浓度（C_i），增加净光合速率（P_n）、蒸腾速率（T_r）、气孔导度（G_s）、最大量子产额（F_v/F_m）、最大光合潜能（F_v/F_o）、电子传输活性（F_m/F_o）及SPAD值含量，在以50 µmol·L⁻¹浓度处理时作用效果最明显，地上部干重、CAT、SOD、MDA、相对电导率、P_n、C_i、SPAD值、F_v/F_m、F_v/F_o、F_m/F_o呈显著水平，Pro、T_r、G_s呈极显著水平。结论施加外源MT可以通过提高青椒幼苗抗氧化酶活性、光合速率，降低细胞膜质过氧化并调节渗透物质含量来减轻低温冷害的影响，从而提高青椒幼苗耐冷性，且以50 µmol·L⁻¹浓度进行喷施最佳。
- 青椒 /
- 幼苗 /
- 褪黑素 /
- 低温胁迫 /
- 生理机制
Abstract: Objective Physiological mechanism and exogenous melatonin (MT) application to improve cold tolerance of green pepper seedlings were investigated. Method Seedlings of green pepper cultivar, Britney, were planted in matrix pots and sprayed with a MT solution of 0 (CK), 25, 50, 75, or 100 µmol·L⁻¹. Effects on the growth, antioxidant enzyme activity, osmotic regulatory substances, and photosynthetic characteristics of the seedlings under chilling at 4 ℃ were determined. Result Comparing to CK, spraying MT increased the contents of catalase (CAT), superoxide dismutase (SOD), and proline (Pro), as well as the net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s), maximum quantum yield (F_v/F_m), maximum photosynthetic potential (F_v/F_o), electron transport activity (F_m/F_o), leaf SPAD, while decreased malondialdehyde (MDA), relative electrical conductivity, and intercellular CO₂ concentration in leaf. At 50 µmol·L⁻¹ level, the MT spray showed the greatest effect in alleviating cold injury on the seedlings. The treatment also allowed the underground dry weight, CAT, SOD, MDA, relative conductivity, P_n, C_i, leaf SPAD, F_v/F_m, F_v/F_o, and F_m/F_oto reach significant heights and Pro, T_r, and G_s at extremely significant levels. Conclusion The exogenous MT spray mitigated injury by chilling temperature and improved cold tolerance of the green pepper seedlings through mechanisms such as a heightened antioxidant enzyme activity and photosynthetic rate as well as suppressed membrane lipid peroxidation and regulated osmotic substances. The optimum MT application on the green peeper plants was determined to be at 50 µmol·L⁻¹.
- Green pepper /
- seedlings /
- melatonin /
- cold stress /
- physiological mechanism

HTML全文

图 1 褪黑素对低温胁迫下青椒幼苗叶片光合作用的影响

不同小写字母表示在P＜0.05水平差异显著；不同大写字母表示在P＜0.01水平差异极显著。

Figure 1. Effect of MT spray on photosynthesis of green pepper leaves under chilling at 4 ℃

The different lowercase letters indicate significant differences within the row at the 0.05 probability level; the different capital letters indicate highly significant differences within the row at the 0.01 probability level.

下载: 全尺寸图片幻灯片

图 2 褪黑素对低温胁迫下青椒幼苗叶片SPAD值的影响

Figure 2. Effect of MT spray on leaf SPAD of green pepper seedlings under chilling at 4 ℃

下载: 全尺寸图片幻灯片

表 1 MT对低温胁迫下青椒幼苗生物量的影响

Table 1. Effect of MT spray on biomass of green peppers under chilling at 4 ℃

MT浓度 Melatonin concentration/（µmol·L⁻¹）	地上部鲜重 Above ground fresh weight/g	地上部干重 Above ground dry weight/g	地下部鲜重 Under ground fresh weight/g	地下部干重 Under ground dry weight/g	干重根冠比 root-shoot ratio
0(CK)	4.85±0.47 a	0.52±0.02 b	2.14±0.14 a	0.17±0.02 a	0.33±0.05 a
25	4.87±0.22 a	0.53±0.01 b	2.18±0.15 a	0.18±0.02 a	0.34±0.03 a
50	5.03±0.23 a	0.63±0.02 a	2.54±0.39 a	0.23±0.06 a	0.36±0.01 a
75	4.94±0.49 a	0.62±0.06 a	2.50±0.48 a	0.22±0.05 a	0.35±0.06 a
100	4.91±0.17 a	0.58±0.01 ab	2.26±0.07 a	0.20±0.02 a	0.35±0.02 a
同列数据后不同小写字母表示在P＜0.05水平差异显著。 The lowercase letters indicate significant differences within the row at the 0.05 probability level.

下载: 导出CSV

表 2 MT对低温胁迫下青椒幼苗叶片生理特性的影响

Table 2. Effect of MT spray on physiological characteristics of green pepper leaves under chilling at 4 ℃

MT浓度 Melatonin concentration/ （µmol·L⁻¹）	CAT Catalase/ （U·g⁻¹·min⁻¹）	SOD Superoxide dismutase/ （U·g⁻¹）	Pro Proline/ （µg·mL⁻¹）	MDA Malonaldehyde/ （nmol·g⁻¹）	相对电导率 Relative conductivity/%
0(CK)	1518.7±20.34 d	15.3±3.47 d	19.2±0.67 b	167.9±3.89 a	27.0±1.18 a
25	2305.2±106.60 c	33.5±3.74 c	19.5±0.49 b	134.1±8.03 b	23.4±0.88 b
50	5021.7±283.33 a	179.7±3.26 a	26.7±0.56a^**	104.4±2.61 c	15.0±1.55 c
75	4278.2±436.45 b	68.6±7.65 b	21.4±0.99 b	117.2±3.81 c	22.0±0.75 b
100	1728.9±55.50 cd	32.3±3.27 c	19.6±1.16 b	138.3±9.20 b	24.7±1.83 ab
小写字母表示在P＜0.05水平差异显著；表示在P＜0.01水平差异极显著。下同 The lower case letters indicate significant differences within the row at the 0.05 probability level; Double asterisks () indicate highly significant differences within the row at the 0.01 probability level. Same as below

下载: 导出CSV

表 3 褪黑素对低温胁迫下青椒幼苗叶片叶绿素荧光参数的影响

Table 3. Effect of MT spray on chlorophyll fluorescence parameters of green pepper leaves under chilling at 4 ℃

MT浓度 Melatonin concentration/ （µmol·L⁻¹）	最大量子产额F_v/F_m Maximum quantum yield	最大光合潜能F_v/F_o Maximum light and potential	电子传输活性F_m/F_o Electron transport activity
CK	0.56±0.02 b	1.28±0.11 b	2.28±0.11 b
25	0.57±0.02 b	1.30±0.09 b	2.30±0.09 b
50	0.62±0.01 a	1.61±0.05 a	2.61±0.05 a
75	0.59±0.01 ab	1.39±0.01 b	2.35±0.02 b
100	0.57±0.02 b	1.30±0.11 b	2.30±0.11 b

下载: 导出CSV

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