绿色优质两系杂交稻B两优164的选育

叶仰东; 唐江霞; 林成豹; 邓则勤; 梁水金; 苏荣理; 黄显波

doi:10.19303/j.issn.1008-0384.2022.01.003

绿色优质两系杂交稻B两优164的选育

doi: 10.19303/j.issn.1008-0384.2022.01.003

三明市农业科学研究院，福建三明　365051

基金项目: 福建省科技计划农业引导性项目（2019N0043）；福建省种业创新与产业化工程项目（zycxny2021004）

详细信息

作者简介:
叶仰东（1990−），男，硕士，助理研究员，研究方向：水稻遗传育种（E-mail：245037795@qq.com）

通讯作者:
黄显波（1964−），男，研究员，研究方向：水稻遗传育种（E-mail:huangxb_2000@163.com）

中图分类号: S 511
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出版历程
- 收稿日期: 2021-08-24
- 修回日期: 2021-11-21
- 网络出版日期: 2022-02-07
- 刊出日期: 2022-01-28

Breeding of Green and High-quality Two-line Hybrid Rice B Liangyou 164

Sanming Academy of Agricultural Sciences, Sanming, Fujian,　365051, China

摘要

摘要: 目的选育优质、抗病杂交稻新品种，满足市场对优质、抗病水稻品种的需求。方法以抗病恢复系中组14为母本，授以强恢复系明恢863的花粉，后代经多年多代在福建三明沙县琅口和海南三亚福建南繁基地来回穿梭加代育种，以及在福建将乐黄潭、上杭茶地等地进行稻瘟病抗性鉴定，从中筛选出抗稻瘟病的优良单株进行加代，于2011年育成优势强、群体整齐一致的抗病恢复系明恢164；2014年夏季在沙县用Bph68S与明恢164进行制种（B两优164）。B两优164于2015—2016年参加福建省晚稻品比试验，2017—2018年参加福建省晚稻区域试验、生产试验，2018—2019年参加长江中下游中籼迟熟组联合体区域试验、生产试验。结果 B两优164在福建省晚稻区域试验中平均产量达8 312.18 kg·hm⁻²，比对照宜优673增产8.94%；在长江中下游中籼迟熟组联合体区域试验中平均产量达9 867.15 kg·hm⁻²，比对照丰两优四号增产5.68%；于2019年通过福建省农作物品种委员会审定（闽审稻20190024），2020年通过国家农作物品种委员会审定（国审稻20200221）。结论 B两优164是稳产、高产、中抗稻瘟病、中抗褐飞虱的优质稻品种，其米质达国标三级优质稻品种品质标准，可在长江中下游作中稻和在福建全省作晚稻种植。
- 两系杂交稻 /
- B两优164 /
- 选育
Abstract: Objective A high-quality, disease-and-pest-resistant hybrid rice variety was bred to meet the market demand for green and quality food. Method The disease- and pest-resistant Zhongzu 14 as the female parent and the quality restorer Minghui 863 line as the male parent were used for the hybridization. The time required for the continuous self-crossing process was shortened by conducting part of the experiment in winter in warmer southern province of Hainan, instead of Fujian. To ensure a stable blast resistance, only the progenies confirmed to meet the quality requirement were selected for the proceeding test. A strong on heterosis, uniform in population, and disease-resistant restorer line, Minghui 164, was thus obtained in 2011. It was crossed with Bph68S, a planthoppers-resistant two-line genic male sterile line, to arrive at a new two-line hybrid indica rice named B Liangyou 164 in the summer of 2014. Subsequently, the new hybrid rice was submitted for evaluation at the Provincial Late Variety Comprehensive Trial in 2015-2016, the Regional Test and Production Trial in 2017-2018, and the Regional and Production Trial of Middle and Lower Yangtze River in 2018-2019. Result The average yield of B Liangyou 164 in the regional test in Fujian reached 8 312.18 kg·hm⁻², which was 8.94% higher than that of the reference variety, Yiyou 673, and in the Yangtze River 9 867.15 kg·hm⁻², which was 5.68% higher than that of the reference variety, Fengliangyou No.4. The newly bred variety was certified by the Fujian Province Crop Variety Approval Committee as well as the National Crop Variety Approval Committee in 2020. Conclusion B Liangyou 164 was a stable high-yield hybrid rice variety with moderate resistance to rice blast and brown planthopper. It met the Grade-3 international standard for high quality rice as stipulated by the Ministry of Agriculture and, consequently, considered appropriate for cultivation as mid-season rice in the middle and lower reaches of the Yangtze River and as late-season variety in Fujian.
- Two-line hybrid rice /
- B Liangyou 164 /
- breeding

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图 1 明恢164的选育过程

Figure 1. Breeding process of Minghui 164

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图 2 B两优164的选育过程

Figure 2. Breeding process of B Liangyou 164

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表 1 B两优164参加福建省及长江中下游新品种区域试验的产量表现

Table 1. Yield performance of B Liangyou 164 at Fujian and Middle and Lower Yangtze River Regional Trials

试验类型 Type of test	年份 Year	产量幅度 Range of yield/ (kg·hm⁻²)	平均产量 Average yield/ (kg·hm⁻²)	比对照增产 Compared with CK/ (kg·hm⁻²)	比对照增产率 Ratio compared with CK/%	试验点数 Number of test points	参试品种数 Number of varieties tested
福建区试 Regional trial in Fujian	2017	6 510.00～10 597.50	8 424.60**	999.60	13.46	10	11
	2018	5 584.50～9 907.50	8 199.75	347.25	4.42	12	12
	平均 Average		8 312.18	673.43	8.94
长江中下游区试 Regional trial in the Middle and Lower Yangtze River	2018	7 984.95～11 299.95	9 601.05	381.68	4.14	17	9
	2019	8 575.05～11 715.00	10 133.25**	678.83	7.18	17	13
	平均 Average		9 867.15	530.26	5.66
注：*表示与对照品种在1%水平上有显著性差异，表示与对照品种在5%水平上有显著性差异。 Note: *Significant at confidence level of 1%; significant at confidence level of 5%.

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表 2 B两优164参加福建省晚稻生产试验中的产量表现

Table 2. Yield performance of B Liangyou 164 in production trials for late-season rice varieties in Fujian

试验点 Location	产量 Yield/ (kg·hm⁻²)	比对照增产 Compared with CK/ (kg·hm⁻²)	比对照增产率 Ratio compared with CK/%
福州 Fuzhou	7 905.00	726.00	10.10
石狮 Shishi	7 486.50	343.50	4.81
龙海 Longhai	6 739.50	397.50	6.27
上杭 Shanghang	8 141.25	1 220.55	17.64
沙县 Shaxian	7 827.00	436.5	5.91
南平 Nanping	10 107.00	414.00	4.3
宁德 Ningde	6 639.00	-312.00	-4.49
平均 Average	7 834.95	460.88	6.25

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表 3 B两优164参加长江中下游中籼迟熟组生产试验中的产量表现

Table 3. Yield performance of B Liangyou 164 in production trials for late-maturing mid-indica in middle and lower Yangtze River

试验点 Location	产量 Yield/ (kg·hm⁻²)	比对照增产 Compared with CK/ (kg·hm⁻²)	比对照增产率 Ratio compared with CK/%
福建 Fujian	8 290.20	255.50	3.18
随州 Suizhou	11 325.90	358.63	3.27
芜湖 Wuhu	11 178.00	675.32	6.43
江苏 Jiangsu	9 389.55	1 675.49	21.72
湖南 Hunan	9 268.80	641.04	7.43
岳阳 Yueyang	9 547.95	1 715.98	21.91
河南 Henan	10 074.00	1 026.04	11.34
江西 Jiangxi	8 409.00	1 094.92	14.97
平均 Average	9 685.35	930.63	10.63

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表 4 B两优164在福建省区试中的农艺性状表现

Table 4. Agronomic characteristics of B Liangyou 164 exhibited at Fujian regional and production trials on late-season rice varieties

品种 Name of varieties	试验类型 Type of test	年份 Year	生育期 Growth period/d	株高 Plant height/ cm	穗长 Ear length/ cm	有效穗 Effective panicle / (×10⁴穗·hm⁻²)	成穗率 Effective panicle rate/%	每穗总粒数 Spikelets per panicle	每穗实粒数 Grain density	结实率 Seed setting rate/%	千粒重 1 000 grain weight/g
B两优164 B Liangyou 164	区试 Regional trial	2017	126.4	107.6**	23.1**	267	64.9	155.0	127.4	82.2	28.0*
		2018	126.6	109.2*	22.7**	250.5	62.4	148.3	123.1	83.0	30.1**
		平均 Average	126.5	108.4	22.9	258.8	63.7	151.7	125.3	82.6	29.1
宜优673 Yiyou 673(CK)	区试 Regional trial	2017	124.4	122.9	25.6	246.0	65.7	138.2	114.8	83.1	29.9
		2018	126.8	125.8	25.8	249.0	64.2	137.6	110.7	80.4	32.6
		平均 Average	125.6	124.4	25.7	247.5	65.0	137.9	112.8	81.8	31.3
B两优164 B Liangyou 164	生产试验 Production trial	2018	126.3	109.5	23.5	256.5	—	179.6	145.9	81.1	29.1
宜优673 Yiyou 673(CK)		2018	126.3	125.4	25.6	253.5	—	147.8	120.4	81.8	31.7
注：*表示与对照品种在1%水平上有显著性差异，表示与对照品种在5%水平上有显著性差异。 Note: *Significant at confidence level of 1%; significant at confidence level of 5%.

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表 5 B两优164在长江中下游区试中的农艺性状表现

Table 5. Agronomic characteristics of B Liangyou 164 exhibited at middle and lower Yangtze River regional and production trials on late-season rice varieties

品种 Name of varieties	试验类型 Type of test	年份 Year	生育期 Growth period/d	株高 Plant height /cm	穗长 Ear length /cm	有效穗 Effective panicle no./ (×10⁴ 穗·hm⁻²)	每穗总粒数 Spikelets per panicle	结实率 Seed setting rate/%	千粒重 1 000 grain weight/g
B两优164 B Liangyou 164	区试 Regional trial	2018	129.6	119.0**	25.6	252.0	195.1	82.9	27.7
		2019	130.9	111.4**	25.0	249.0	191.1	84.4	28.1
		平均 Average	130.3	115.2	25.3	250.5	193.1	83.6	27.9
丰两优四号 Feng Liangyou 4 (CK)	区试 Regional trial	2018	131.5	128.9	24.8	234.0	197.4	85.3	28.6
		2019	134.0	122.2	24.9	234.0	187.5	85.1	27.8
		平均 Average	132.8	125.6	24.9	234.0	192.5	85.2	28.2
B两优164 B Liangyou 164	生产试验 Production tria	2019	130.9	111.3	25.0	247.5	191.0	84.3	28.1
丰两优四号 Feng Liangyou 4 (CK)		2019	131.6	—	—	—	—	—	—
注：*表示在1%水平上有显著性差异，表示在5%水平上有显著性差异。 Note: *Significant at confidence level of 1%; significant at confidence level of 5%.

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表 6 B两优164在福建省区试中的抗性表现

Table 6. Resistance of B Liangyou 164 shown at Fujian regional trial

品种 Varieties	年份 Year	点数 Points	田间叶瘟鉴定 Identification of leaf pest in field			田间穗颈瘟鉴定 Identification of ear neck distemper in field			室内鉴定 Identification in greenhouse		综合评价 Comprehensive evaluation
品种 Varieties	年份 Year	点数 Points	发病率Prevalence/ %	病情指数 Disease index	抗性类型 Type of resistance	发病率Prevalence/ %	病情指数 Disease index	抗性类型 Type of resistance	接菌株率 Antibacterial strain rate/ %	抗性类型 Type of resistance	综合评价 Comprehensive evaluation
B两优164 B Liangyou 164	2017	4	1.00	0.24	R	23.13	6.04	MR	100.00	R	MR
	2018	4	1.38	0.50	R	16.38	5.96	MR	87.50	R	R
	平均 Average		1.19	0.37	R	19.76	6.00	MR	93.75	R	MR
宜优673 Yiyou 673(CK)	2017	4	56.75	38.96	S	67.50	45.64	S	42.86	MS	S
	2018	4	38.38	21.82	MS	34.25	16.11	MS	25.00	S	S
	平均 Average		47.57	30.39	S	50.88	30.88	S	33.93	S	S

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