不同耐盐性水稻材料对盐胁迫的生理响应及分子机制

徐淑英

doi:10.19303/j.issn.1008-0384.2022.009.015

不同耐盐性水稻材料对盐胁迫的生理响应及分子机制

doi: 10.19303/j.issn.1008-0384.2022.009.015

徐淑英

福建省龙岩市农业科学研究所，福建　龙岩　364000

基金项目: 福建省科技计划星火项目（2018S0025）

详细信息

作者简介:
徐淑英（1964−），女，高级农艺师，主要从事水稻遗传育种与良种推广（E-mail：lyghlanwu@126.com）

中图分类号: S 511
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出版历程
- 收稿日期: 2022-06-13
- 录用日期: 2022-09-19
- 修回日期: 2022-07-31
- 网络出版日期: 2022-10-21
- 刊出日期: 2022-09-30

Physiological Response and Molecular Mechanism of Rice to Salt-stress

XU Shuying

Longyan Agricultural Research Institute, Longyan, Fujian　364000, China

摘要

摘要: 目的研究不同耐盐性水稻材料应答高盐胁迫的生理及分子机理，对筛选和培育耐盐水稻新品种具有重要的参考价值。方法分别以耐盐水稻品系X1、X2、X3和盐敏感水稻品系X20、X30为试验材料，分析其在高盐胁迫（200 mmol·L⁻¹ NaCl的1/2KB溶液）条件下的生理代谢及耐盐相关功能基因表达差异。结果在高盐胁迫条件下，耐盐水稻材料体内脯氨酸及可溶性糖含量、超氧化物歧化酶及过氧化氢酶的活性，以及耐盐相关功能基因OsP5CS1、OsProt、OsCu/Zn-SOD、OsAPX2、OsNCED3及OsNCED5的表达水平明显高于盐敏感型，而丙二醛和过氧化氢含量明显低于盐敏感型。结论耐盐性水稻材料主要通过提高渗透调节能力、活性氧清除能力及耐盐相关功能基因的表达来提高其耐盐性。
- 水稻 /
- 高盐胁迫 /
- 渗透调节 /
- 活性氧清除 /
- 耐盐基因
Abstract: Objective Physiological response and molecular mechanism of different salt-tolerant rice lines in dealing with high salt stress were studied to facilitate screening and breeding resistant varieties. Methods Salt-tolerant rice X1, X2, and X3 as well as salt-sensitive X20 and X30 were treated by a high salt condition of 1/2KB solution with 200 mmol·L⁻¹NaCl. The physiological metabolism and expression of functional genes of the two contrasting groups of rice were compared. Results Under the stress, the proline and soluble sugar contents, superoxide dismutase and catalase activities, and expressions of OsP5CS1, OsProt, OsCu/Zn-SOD, OsAPX2, OsNCED3, and OsNCED5 of the salt-tolerant rice were significantly higher, whereas the malondialdehyde and hydrogen peroxide contents significantly lower, than those of the salt-sensitive counterparts. Conclusion The salt-tolerant rice lines resisted the high salt exposure mainly by increasing osmotic regulation, activating oxygen scavenging, and accentuating functional gene expression of the plants.
- Rice /
- salt stress /
- osmotic regulation /
- reactive oxygen scavenging /
- salt-tolerance-related functional genes

HTML全文

图 1 正常生长条件及高盐胁迫条件下耐盐性品系和盐敏感型植株之间脯氨酸(A)和可溶性糖(B)含量比较

图中不同小写字母表示不同材料间差异显著（P＜0.05）。下图同。

Figure 1. Proline (A) and sugar (B) in salt-tolerant and salt-sensitive rice plants under salt-stress or normal conditions

Data with different lowercase letters represent significant difference at P＜0.05 among different rice lines. The same for the following.

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图 2 正常生长条件及高盐胁迫处理条件下耐盐品系与盐敏感型品系之间H₂O₂ (A)和MDA (B)含量比较

Figure 2. H₂O₂ (A) and MDA (B) in salt-tolerant and salt-sensitive rice plants under salt-stress or normal conditions

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图 3 正常生长条件及高盐胁迫条件下耐盐品系与盐敏感型植株之间CAT (A)和SOD(B)活性比较

Figure 3. CAT (A) and SOD (B) activities of salt-tolerant and salt-sensitive rice plants under salt-stress or normal conditions

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图 4 正常生长条件及高盐胁迫条件下耐盐相关基因在耐盐与盐敏感型植株之间的表达比较

Figure 4. Expressions of salt-stress-related genes of salt-tolerant and salt-sensitive rice plants under salt-stress or normal conditions

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表 1 实时定量PCR分析所用引物

Table 1. Primers used in qRT-PCR analyses

名称 Name	引物序列 Primers sequence
OsActin	F：5'-AGCTATCGTCCACAGGAA -3'
	R：5'-ACCGGAGCTAATCAGAGT -3'
OsP5CS1	F： 5'-TCTGCTCAGTGATGTGGATG -3'
	R：5'-CCTACACGAGATTTGTCTCC -3'
OsProt	F：5'-TCGTTCTGACAACTGGGGTGA-3'
	R：5'-TGTCGTTTGCCTCCGATTTC-3'
OsAPX2	F：5'- AACTTCCCATCCTCTCCTAC -3'
	R：5'- CTCTCCTTGTGGCATCTTCC -3'
OsCu/Zn-SOD	F：5'-TATCATCGTCAGGTCAGGCA -3'
	R：5'- ACACTTCAGCTGCAACTTGC -3'
OsNCED3	F：5'- CTCACATACAGCGGCAGCAC -3'
	R：5'- CGCTCGAGGACATTCGCCAC -3'
OsNCED5	F：5'- CCCAGCTTGAAGCTTTTGCT -3'
	R：5'- ACAACACTGCAACTATCCCTATCACT-3'

下载: 导出CSV

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