宁夏银川地区番茄猝倒病病原菌的鉴定

杨宏; 王晓敏; 宋丽娜; 刘文娟; 李利平; 程国新; 郭猛; 付金军; 胡新华

doi:10.19303/j.issn.1008-0384.2022.009.013

宁夏银川地区番茄猝倒病病原菌的鉴定

doi: 10.19303/j.issn.1008-0384.2022.009.013

杨宏^1,,
王晓敏^{1, 2, 3, 4, ,},
宋丽娜¹,
刘文娟¹,
李利平¹,
程国新^{1, 2, 3, 4},
郭猛^{1, 2, 3, 4},
付金军⁵,
胡新华⁵

1.
宁夏大学农学院，宁夏　银川　750021
2.
宁夏现代设施园艺工程技术研究中心，宁夏　银川 750021
3.
宁夏优势特色作物现代分子育种重点实验室，宁夏　银川　750021
4.
宁夏设施园艺（宁夏大学）技术创新中心，宁夏　银川　750021
5.
宁夏巨丰种苗有限责任公司，宁夏　银川　750021

基金项目: 宁夏回族自治区农业特色优势产业育种专项（NXNYYZ20200101）；宁夏回族自治区重点研发计划（重大）重点项目（2019BBF02022）

详细信息

作者简介:
杨宏（1998-），男，硕士研究生，研究方向：蔬菜生物技术与遗传育种（E-mail：2835377608@qq.com）

通讯作者:
王晓敏（1981-），女，副教授，研究方向：蔬菜生物技术与遗传育种（E-mail：wangxiaomin_1981@163.com ）

中图分类号: S 436.412
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出版历程
- 收稿日期: 2022-03-02
- 修回日期: 2022-05-09
- 网络出版日期: 2022-08-29
- 刊出日期: 2022-09-30

Pathogens of Tomato Damping-off Disease in Yinchuan, Ningxia

YANG Hong^1
,,
WANG Xiaomin^{1, 2, 3, 4
, ,},
SONG Lina¹,
LIU Wenjuan¹,
LI Liping¹,
CHENG Guoxin^{1, 2, 3, 4},
GUO Meng^{1, 2, 3, 4},
FU Jinjun⁵,
HU Xinhua⁵

1.
School of Agriculture, Ningxia University, Yinchuan, Ningxia　750021, China
2.
Ningxia Modern Facility Horticulture Engineering Technology Research Center, Yinchuan, Ningxia　750021, China
3.
Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan, Ningxia　750021, China
4.
Ningxia Facility Horticulture (Ningxia University) Technology Innovation Center, Yinchuan, Ningxia　750021, China
5.
Ningxia Jufeng Seedlings Co., Ltd.， Yinchuan, Ningxia　750021，China

摘要

摘要: 目的探明宁夏银川地区番茄猝倒病的病原菌，为该病的防治及抗病育种工作奠定基础。方法以番茄猝倒病病株为材料，通过常规组织分离法分离病原菌，从形态学、致病性测定和rDNA-ITS序列分析三方面对其进行鉴定。结果共分离出2种不同形态的病原菌，1号分离物孢子囊卵圆形或近球形，有乳突，孢子囊脱落具长柄，初步判断其为辣椒疫霉；2号分离物藏卵器为球形，柄较直，雄器侧生，宽棍棒状或近柠檬形，卵孢子球形，平滑，不满器，初步判断其为瓜果腐霉。在番茄子叶期分别接种两种病原菌后，番茄苗均发生猝倒，在接种部位出现暗褐色斑点和茎缢缩等与自然发病一致的病害症状，表明两种病原菌是引起番茄猝倒病的病原菌。利用两种分离物的ITS序列分别构建系统进化树，1号分离物与辣椒疫霉处于同一分支；2号分离物与瓜果腐霉处于同一分支。结论引起银川市番茄猝倒病的病原菌主要为辣椒疫霉和瓜果腐霉。
- 番茄 /
- 银川地区 /
- 猝倒病 /
- 病原菌 /
- 鉴定
Abstract: Objective To find out the pathogen of tomato damping-off in Yinchuan area of Ningxia, and lay a foundation for disease control and disease resistance breeding. Methods Possible pathogenic microbes of the disease on tomatoes were isolated by the conventional tissue isolation method and identified based on their morphology, pathogenicity, and rDNA-ITS sequence. Result Of the two suspected species, Pathogen No. 1 had oval or nearly spherical sporangia with papillae and long stalks. It was preliminarily determined as Phytophthora capsici. The spherical ovipositor of Pathogen No. 2 had straight stalks with spherical, smooth oospores that did not fully fill the sacs and the wide rod- or lemon-like-shaped male organ grew laterally. It was presumed to be Pythium aphanidermatum. After inoculation on tomato cotyledons the inoculation of either of the two isolates caused the seedling stem constriction and plant collapse showing the typical symptoms of dark brown spots at infected sites. The phylogenetic trees constructed based on the ITS sequences of the two isolates confirmed No. 1 to belong in the branch of P. capsici, and No. 2 of P. aphanidermatum. Conclusion The pathogens that caused damping-off on tomato plants in Yinchuan were positively identified to be strains of P. capsici and P. aphanidermatum.
- Tomato /
- Yinchuan area /
- damping-off disease /
- pathogen /
- identification

HTML全文

图 1 1号病原菌形态

a：菌落；b：孢子囊；c：菌丝体；d：菌丝； M：乳突；L：长柄；Z：游动孢子；H：菌丝；HS：隔膜。

Figure 1. Morphological characteristics of Isolate No. 1

a: Colony; b: Sporangia; c: Mycelium; d: Mycelia; M: Mastoid; L: Long stalk; Z: Zoospore; H: Hyphae; HS: Hyphal septum.

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图 2 2号病原菌形态

a：菌落；b：有性器官；c和d：无性繁殖结构；A：雄器；O：藏卵器；OS：卵孢子；S：孢子囊；V：泡囊。

Figure 2. Morphological characteristics of Isolate No. 2

a: Colony; b: Sexual organ; c and d: Asexual reproduction structure; A: Antheridia; O: Oogonia; OS: Oospore; S: Sporangium; V: Vesicle.

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图 3 病原菌致病性测定结果

a：对照；b：接种1号分离物；c：接种2号分离物。

Figure 3. Pathogenicity of isolates

a: CK; b:Inoculation of Isolate No. 1 ; c: Inoculation of Isolate No. 2.

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图 4 PCR产物琼脂糖凝胶电泳检测

a：1号分离物条带；b：2号分离物条带；M：Marker；1和2：目的条带。

Figure 4. Agarose gel electrophoresis detection of PCR products

a: Separation band of Isolate No. 1; b: Separation band of Isolate No. 2; M: Marker; 1 and 2: Target bands.

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图 5 引起番茄猝倒病的1号分离物系统进化树

红色三角形标注的是1号分离物。

Figure 5. Phylogenetic tree of Pathogen No. 1

Red triangle indicates Pathogen No. 1.

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图 6 引起番茄猝倒病的2号分离物系统进化树

红色三角形标注的是2号分离物。

Figure 6. Phylogenetic tree of Pathogen No. 2

Red triangle indicates Pathogen No. 2.

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