玉米大斑病菌热激蛋白Hsp70的鉴定和结构分析

张淑红; 范永山

doi:10.19303/j.issn.1008-0384.2022.009.010

玉米大斑病菌热激蛋白Hsp70的鉴定和结构分析

doi: 10.19303/j.issn.1008-0384.2022.009.010

张淑红,
范永山^,

唐山师范学院生命科学系/唐山市农业病原真菌与毒素重点实验室，河北　唐山　063000

基金项目: 河北省自然科学基金项目（C2019105055）；唐山市人才项目（A202007009）；唐山师范学院科研项目（2021B30、2021B34）

详细信息

作者简介:
张淑红（1978–），女，硕士，副教授，研究方向：微生物遗传（E-mail：zsh3535@163.com）

通讯作者:
范永山（1971–），男，博士，教授，研究方向：真菌遗传学（E-mail：fanyongshan@126.com）

中图分类号: S 432.1
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出版历程
- 收稿日期: 2022-05-04
- 修回日期: 2022-05-17
- 刊出日期: 2022-09-30

Identification and Characterization of Heat Shock Protein Hsp70 in Setosphaeria turcica

ZHANG Shuhong,
FAN Yongshan^,

Department of Life Sciences, Tangshan Normal University/Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins, Tangshan, Hebei　063000, China

摘要

摘要: 目的对玉米大斑病菌Hsp70（Setosphaeria turcica Hsp70，StHsp70）基因家族进行结构鉴定和功能分析，为进一步阐明StHsp70在玉米大斑病菌生长发育和致病过程中的作用奠定基础。方法利用玉米大斑病菌基因组数据库获得StHsp70基因家族成员，利用生物信息学方法进行理化性质、亚细胞定位、系统进化、保守基序和结构域分析，构建三级结构模型，并预测启动子等顺式作用元件。结果 StHsp70基因家族包括11个成员，分别为StHsp70-1～StHsp70-11，多数定位于细胞质，其次为内质网、线粒体和细胞核。系统进化分析结果表明，StHsp70家族成员可分为7类，其中Class A～F分别与酿酒酵母（Saccharomyces cerevisiae）热激蛋白SSA、SSB、SSC、KAR2、SSE、SSZ高度同源，而Class G在酵母中未发现其同源蛋白。结构预测分析发现，StHsp70家族成员都含有保守基序Motif 5，而Motif 6只存在于Class A～D中，Class G仅含有2～4种基序，与其他StHsp70家族成员存在显著差异。N端的NBD结构域类型在玉米大斑病菌和酵母不同Hsp70类型间存在显著差异，可能与亚细胞定位有关。不同类型StHsp70的C末端结构和延伸性变化较大，特别是Class G的StHsp70与其他类型具有显著区别，可能与底物的多样性有关。结论玉米大斑病菌Hsp70家族11个成员可以分为7类，其中Class G的4个成员在理化性质和结构特征上都与其他成员具有显著的区别，表明StHsp70属于多功能分子伴侣家族。
- 玉米大斑病菌 /
- Hsp70 /
- 结构分析 /
- 功能预测
Abstract: Objective Identification and characteristics of Hsp70 family in Setosphaeria turcica were studied to facilitate elucidating their roles in the growth, development, and pathogenicity of the microbe. Methods Members of StHsp70 family were identified from the S. turcica genome database. Physicochemical properties, subcellular localization, phylogenetic evolution, conserved motifs, and domains of the genes analyzed by bioinformatics methods. Results Eleven members, StHsp70-1 to StHsp70-11, were identified from the database. Most of them were predicated to locate in the cytoplasmic as well as in endoplasmic reticulum, mitochondrial, and nucleus in lesser amounts. The phylogenetic analysis divided the members into 7 categories including Classes A−F that showed a high homology with the heat shock proteins SSA, SSB, SSC, KAR2, SSE, and SSZ of Saccharomyces cerevisiae, respectively, and Class G that had none with what were found in yeasts. All StHsp70s contained conserved motif 5, but motif 6 existed in Class A−D only. Class G had only motifs 2−4 making the class significantly different from the others. The variations in subcellular localization might be the reason of the significant N-terminal differences in the NBD domains of the Hsp70 classes of S. turcica from those of yeast. Whereas the considerably varied C-terminal structure and extensibility among the classes, especially on the StHsp70s of Class G, might contribute to the diversity of substrates. Conclusion The 11 members of Hsp70 family of S. turcica could be divided into 7 classes with 4 members in Class G being significantly different from the others in physicochemical properties and structure. It indicated that the genes were of multifunctional molecular chaperones.
- Setosphaeria turcica /
- Hsp70 /
- structure analysis /
- function prediction

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图 1 玉米大斑病菌Hsp70家族进化树

Figure 1. Phylogenetic tree of Hsp70 family in S. turcica

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图 2 玉米大斑病菌Hsp70基因家族进化树（a）、保守基序（b）和基因结构（c）

Figure 2. Evolution tree (a), conserved motifs (b), and gene structure (c) of StHsp70 family

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图 3 玉米大斑病菌Hsp70的NBD结构域分析

Figure 3. NBD domains of StHsp70 family

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图 4 玉米大斑病菌Hsp70的三级结构

蓝色代表N端，红色代表C端。

Figure 4. Tertiary structure of StHsp70 family

Blue represents N-end; red represents C-end.

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表 1 玉米大斑病菌Hsp70基因家族信息

Table 1. Information on Hsp70 gene family in S. turcica

基因 Gene	染色体定位 Chromosome location	蛋白质ID Protein ID	氨基酸数量 The amount of amino acid	分子量 Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
Hsp70-1	scaffold_1	133966	645	70064	5.10	细胞质Cytoplasmic
Hsp70-2	scaffold_1	86462	562	62877	8.06	细胞质Cytoplasmic 线粒体Mitochondrial
Hsp70-3	scaffold_3	163910	729	80129	8.30	细胞核Nuclear
Hsp70-4	scaffold_3	163930	1025	111897	5.94	内质网Endoplasmic reticulum 细胞质Cytoplasmic
Hsp70-5	scaffold_4	164298	671	72827	5.62	线粒体Mitochondrial
Hsp70-6	scaffold_4	1352336	748	84696	6.14	细胞质Cytoplasmic
Hsp70-7	scaffold_8	122795	571	61047	5.05	细胞质Cytoplasmic
Hsp70-8	scaffold_10	166606	629	69891	6.49	细胞质Cytoplasmic
Hsp70-9	scaffold_14	168163	614	66956	5.35	细胞质Cytoplasmic
Hsp70-10	scaffold_16	168574	674	73861	5.09	内质网Endoplasmic reticulum
Hsp70-11	scaffold_18	168841	722	80441	5.23	细胞质Cytoplasmic

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