Breeding of High-quality, High-yield, Early-maturing Hybrid Rice Huangyou Yuehesimiao
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摘要:
目的 充分挖掘和利用优良常规稻资源,加强早熟优良杂交水稻不育系的创制,培育杂种优势强、优质、抗病及广适应性的早熟杂交水稻新品种,满足农业种植结构调整对早熟优良杂交水稻品种需求。 方法 以泸香90B为母本,早熟优质常规稻佳辐占为父本杂交,后代经福建建阳和海南三亚两地穿梭加代育种,及福建上杭茶地稻瘟病抗性鉴定,从中筛选出早熟、大粒、优质、抗病的优良单株与Ⅱ-32A测交和多代回交转育,于2013年育成早熟优质高配合力水稻不育系潢达A;2016年春季在海南用潢达A与恢复系粤禾丝苗进行制种配组(潢优粤禾丝苗)。潢优粤禾丝苗于2016–2017年参加福建、海南和江西等多地品比试验,2018–2020年参加福建省早稻组和长江中下游晚籼早熟组联合体区试和生产试验,考察其综合性状表现。 结果 潢优粤禾丝苗在多地品比试验中,平均产量达8100.00 kg·hm−2以上,比对照品种增产4.33%~18.76%。在福建省早稻新品种筛选试验、区试和生产试验中,平均产量分别达8014.50 kg·hm−2、8003.70 kg·hm−2和7444.65 kg·hm−2,比对照品种T78优2155平均增产4.80%、10.93%和8.26%;在长江中下游晚籼早熟组联合体区试和生产试验中,平均产量为9761.25 kg·hm−2和8838.45 kg·hm−2,比对照品种五优308平均增产4.18%和3.54%;于2020年通过福建省农作物品种审定委员会审定(闽审稻20200009),2021年通过国家农作物品种审定委员会审定(国审稻20210328)。 结论 潢优粤禾丝苗是早晚兼用型、高产稳产、抗病的优质杂交水稻品种,其早稻和晚稻米质分别达部颁一等和三等优质稻品种品质标准,可在福建全省作早稻种植和长江中下游双季稻区作晚稻种植,推广应用前景广阔。 Abstract:Objective To fully explore the potential of existing resources, an early-maturing hybrid rice CMS line with much desirable qualities was bred. Method Luxiang 90B as the female parent and the high-quality early-maturing traditional Jiafuzhan as the male parent were applied for the hybridization. To save time, a part of the self-crossing process was conducted in the warmer southern province of Hainan, instead of Fujian, in winter. A blast resistance test was performed at a tea plantation in Shanghang. Hybrids showing the target traits of early maturation, large grains, high quality, and disease resistance were selected to backcross with II -32A for several generations. The final combined line was then crossed with the traditional restorer line Yuehesimiao to yield a 3-line hybrid indica rice for submission to the comparative trials held in different provinces. Result An early-maturing, high-quality, strong combining ability CMS line, Huangda A, was obtained in 2013 to be crossed with Yuehesimiao resulting in a new 3-line hybrid indica rice named Huangyou Yuehesimiao in spring 2016. During 2016–2017, the newly bred rice was submitted to the trials in Hainan and Jiangxi in addition to Fujian for performance evaluation. Subsequently, in 2018–2020, it participated in the trials for screening, regional competition, and production of early-season rice in Fujian as well as early-maturing, late-season indica rice in the middle and lower reaches of Yangtze River to be critically examined in regard to growth period, yield, blast resistance, grain quality, and adaptability of the rice varieties. In the Hainan, Jiangxi, and Fujian trials, Huangyou Yuehesimiao delivered an average yield of more than 8 100.00 kg·hm−2, which was a 4.33% to 18.76% higher than that of the reference variety. In the screening, regional, and production trials on early-season rice, it showed the average yields of 8 014.5 kg·hm−2, 8 003.70 kg·hm−2, and 7 444.65 kg·hm−2, respectively, representing increases of 4.80%, 10.93%, and 8.26%, respectively, in comparison to that of T78 You 2155. Meanwhile, in the regional and production trials on early-maturing, late-season rice in the middle and lower reaches of Yangtze River, the average yields were 9 761.25 kg hm−2 and 8 838.45 kg·hm−2, respectively, with 4.18% and 3.54% increases, respectively, as compared to Wuyou 308. The new variety was certified by the Fujian Province Crop Variety Approval Committee in 2020 and by the National Crop Variety Approval Committee in 2021. Conclusion Huangyou Yuehesimiao was a high-quality, high- and stable-yield, blast resistant hybrid rice suitable for either early or late season cropping. The grain quality met the national standard for Grade-1 early rice and Grade-3 late rice as set by the Ministry of Agriculture. Consequently. Consequently, it was deemed appropriate for early and late-season double cropping rice cultivation in Fujian or areas such as the middle and lower reaches of the Yangtze River. -
Key words:
- hybrid rice /
- early-maturing /
- Huangyou Yuehesimiao /
- breeding
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表 1 潢优粤禾丝苗等杂交组合的农艺性状和产量表现
Table 1. Yield and Agronomic traits of Huangyou Yuehesimiao and other hybrid rice combinations
品种名称
Name of varieties生育期
Growth
period/d株高
Plant
height/cm有效穗数
Effective panicle no./
(×104 ·hm−2)每穗总粒数
Total grain per
panicle结实率
Seed etting
rate/%千粒重
1 000 grain
weight/g产量
Yield/
(kg·hm−2)比CK增产率
Ratio compared
with CK/%比CK2增产率
Ratio compared
with CK2/%潢优粤禾丝苗
Huangyou Yuehesimiao117 103.7 268.9 187.0 90.98 26.8 8657.2 14.25 5.90 潢优308
Huangyou 308116 97.7 286.2 185.9 86.16 26.5 8642.5 14.05 5.72 律优308
Lüyou 308112 95.6 296.7 175.3 82.30 25.0 8325.1 9.87 1.84 清优308
Qingyou 308118 100.2 309.3 178.5 83.10 23.2 8227.4 8.58 0.64 清优粤禾丝苗
Qingyou Yuehesimiao118 104.7 285.7 173.9 84.03 24.6 8186.5 8.04 0.14 五优308
Wuyou 308(CK2)120 107.0 275.7 183.7 88.66 23.8 8175.0 7.89 — 泓优308
Hongyou 308115 102.3 308.7 173.4 77.58 23.5 8105.1 6.96 −0.86 澄优粤禾丝苗
Chengyou Yuehesimiao119 99.3 260.0 163.8 82.07 27.3 8040.0 6.10 −1.65 泓优粤禾丝苗
Hongyou Yuehesimiao119 100.0 287.1 165.4 80.90 26.8 8017.5 5.81 −1.93 律优粤禾丝苗
Lüyou Yuehesimiao113 104.3 235.7 171.6 79.76 26.0 7705.0 1.68 −5.75 澄优308
Chengyou 308114 105.0 288.6 163.2 76.63 23.9 7696.4 1.57 −5.85 金优2155
Jinyou 2155(CK)116 113.3 257.0 157.1 84.14 27.2 7577.5 — −7.31 表 2 潢优粤禾丝苗在福建省区域试验和生产试验产量表现
Table 2. Yield of Huangyou Yuehesimiao in regional and production trials in Fujian
试验类型
Type of trial品种名称
Name of
varieties年份
Year产量
Yield/
(kg·hm−2)比CK1增产率
Ratio
compared
with
CK1/%区域试验
Regional trial潢优粤禾丝苗
Huangyou
Yuehesimiao2018 8140.05 11.41 2019 7867.35 10.45 平均
Average8003.70 10.93 T78优2155
T78you2155
(CK1)2018 7306.65 — 2019 7122.90 — 平均
Average7214.78 — 生产试验
Producing trial潢优粤禾丝苗
Huangyou
Yuehesimiao2019 7444.65 8.26 T78优2155
T78you2155(CK1)2019 6876.45 — 表 3 潢优粤禾丝苗在长江中下游联合体区域试验和生产试验产量表现
Table 3. Yield of Huangyou Yuehesimiao in regional and production trials in middle and lower reaches of Yangtze River
试验类型
Type of trial品种名称
Name of varieties年份
Year产量
Yield/
(kg·hm−2)比CK2增产率
Ratio compared
with CK2/%区域试验
Regional trial潢优粤禾丝苗
Huangyou Yuehesimiao2018 9610.80 3.49 2019 9911.70 4.87 平均
Average9761.25 4.18 五优308
Wuyou 308(CK2)2018 9286.95 — 2019 9451.50 — 平均
Average9369.23 — 生产试验
Producing trial潢优粤禾丝苗
Huangyou
Yuehesimiao2020 8838.45 3.54 五优308
Wuyou 308(CK2)2020 8536.05 — 表 4 潢优粤禾丝苗在福建省和长江中下游区试中的主要农艺性状
Table 4. Main agronomic traits of Huangyou Yuehesimiao displayed in regional trials in Fujian and middle and lower reaches of Yangtze River
试验类型
Type of
trial品种名称
Name of
varieties年份
Year生育期
Growth
period/d有效穗数
Effective panicle no./
(×104·hm−2)株高
Plant
height/cm穗长
Spike
length/cm每穗总粒数
Total grain per
panicle结实率
Seed
setting rate/%千粒重
1000 -grain
weight/g福建区试
Regional trial in
Fujian潢优粤禾丝苗
Huangyou Yuehesimiao2018 127.6 252.0 102.2 22.7 162.3 87.24 26.7 2019 128.7 270.0 94.8 21.8 143.4 84.22 26.7 平均
Average128.2 261.0 98.5 22.3 152.9 85.73 26.7 T78优2155
T78you2155(CK1)2018 127.4 256.5 117.5 24.7 158.8 82.19 26.2 2019 128.6 259.5 107.4 23.5 140.2 82.90 24.9 平均
Average128.0 258.0 112.5 24.1 149.5 82.55 25.6 长江中下游区试
Regional trial in
middle and lower reaches of
Yangtze River潢优粤禾丝苗
Huangyou Yuehesimiao2018 112.5 289.5 106.8 23.4 163.3 87.30 26.5 2019 111.2 283.5 106.9 24.2 178.5 89.10 25.8 平均
Average111.9 286.5 106.9 23.8 170.9 88.20 26.2 五优308
Wuyou 308(CK2)2018 116.6 318.0 108.3 21.1 161.9 84.90 23.7 2019 115.8 294.0 110.4 22.1 178.2 84.20 23.9 平均
Average116.2 306.0 109.3 21.6 170.1 84.50 23.8 表 5 潢优粤禾丝苗在福建省区试中的稻瘟病抗性表现
Table 5. Blast-resistance of Huangyou Yuehesimiao exhibited at regional trial in Fujian
品种名称
Name of
varieties年份
Year田间自然诱发鉴定
Fields natural inducing identification苗期室内鉴定
Indoor identification at seedling stage抗性综合评价
Comprehensive
evaluation of
resistance鉴定点数
Identification
points叶瘟
Leaf blast穗颈瘟
Neck-panicle blast抗性评价
Resistance
evaluation接菌株数
No. of
inoculated
strains抗菌株率
Resistance to
strain rate/%抗性评价
Resistance
evaluation发病率
Incidence
rate/%病情
指数
Disease
index最高级
Top
grade发病率
Incidence
rate/%病情
指数
Disease
index最高级
Top
grade潢优
粤禾丝苗
Huangyou
Yuehesimiao2018 4 4.38 1.12 5 14.00 3.69 5 R 20 75.00 MR MR 2019 4 13.38 4.32 4 23.75 8.45 9 MR 20 78.95 MR MR 平均
Average4 8.88 2.72 5 18.88 6.07 9 MR 20 76.98 MR MR T78优2155
T78you2155(CK1)2018 4 31.25 12.63 7 46.00 26.17 9 S 20 18.75 S S 2019 4 42.13 18.17 8 56.75 25.36 9 S 20 21.05 S S 平均
Average4 36.69 15.40 8 51.38 25.77 9 S 20 19.90 S S 表 6 潢优粤禾丝苗在长江中下游联合体区试中的主要病虫抗性表现
Table 6. Major disease and insect resistance of Huangyou Yuehesimiao identified in regional trial in middle and lower reaches of Yangtze River
品种名称
Name of varieties年份
Year稻瘟病 Rice blast 白叶枯病抗性等级
Bacterial
blight grade褐飞虱抗性等级
Brown
planthopper grade综合指数 Comprehensive index 穗瘟损失率最高级
Highest loss rate of panicle blast浙江
Zhejiang湖南
Hunan湖北
Hubei安徽
Anhui福建
Fujian江西
Jiangxi平均
Average潢优粤禾丝苗
Huangyou Yuehesimiao2018 4.00 4.00 4.00 5.80 2.00 5.75 4.30 5 7 7 2019 4.00 4.25 5.25 4.00 3.50 4.00 4.20 5 7 9 五优308
Wuyou 308(CK2)2018 3.80 4.50 4.50 6.80 8.00 6.75 5.70 9 9 9 2019 4.50 4.75 5.50 5.50 4.00 7.00 5.20 7 7 9 表 7 潢优粤禾丝苗与T78优2155、五优308的稻米品质比较(2019年)
Table 7. Grain quality of Huangyou Yuehesimiao, T78you 2155, and Wuyou 308 in 2019
类型
Type品种名称
Name of varieties糙米率
Brown rice
percentage/
%精米率
Milled rice
percentage/
%整精米率
Head rice
percentage/
%粒长
Grain
length/
mm长宽比
Ratio of
grain length
to width垩白度
Chalkiness/
%透明度
Transparency碱消值
Alkali
value胶稠度
Gel
consistency/
mm直链淀粉
Amylose
content/
%福建区试
Regional trial
in Fujian潢优粤禾丝苗
Huangyou
Yuehesimiao82.6 75.0 67.7 7.1 3.2 1.0 1.0 7.0 78 17.7 T78优2155
T78you2155
(CK1)81.3 74.0 65.7 6.4 2.6 8.9 2.0 4.7 58 21.8 长江中下游区试
Regional trial in
middle and
lower reaches of
Yangtze river潢优粤禾丝苗
Huangyou
Yuehesimiao81.1 72.5 63.5 6.8 3.4 3.2 1.0 6.6 53 16.6 五优308
Wuyou 308(CK2)80.1 72.9 34.6 6.2 2.9 5.7 2.3 5.1 45 19.6 -
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