福建甜椒蓟马发生情况及蜡蚧轮枝菌对蓟马优势种的致病力

黄鹏; 张杰; 陈汉鑫; 蓝炎阳; 姚锦爱; 余德亿

doi:10.19303/j.issn.1008-0384.2022.004.012

福建甜椒蓟马发生情况及蜡蚧轮枝菌对蓟马优势种的致病力

doi: 10.19303/j.issn.1008-0384.2022.004.012

1.
福建省作物有害生物监测与治理重点实验室/福建省农业科学院植物保护研究所/福建省作物有害生物绿色防控工程研究中心，福建　福州　350013
2.
漳州市农业科学研究所，福建　漳州　363005
3.
漳州农业科技园区研发中心，福建　漳浦　363204

基金项目: 福建省科技计划引导性项目（2021N0050）；福建省农业科学院科技创新团队建设项目（CXTD2021005-2）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021011）

详细信息

作者简介:
黄鹏（1984−），男，硕士，助理研究员，研究方向：害虫生物防治（E-mail：375202928@qq.com）

通讯作者:
余德亿（1972−），男，研究员，研究方向：病虫害生物防治（E-mail：yudy_2004@126.com）

中图分类号: S 436.3;S 476.1
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出版历程
- 收稿日期: 2021-11-23
- 修回日期: 2021-12-17
- 网络出版日期: 2022-02-07
- 刊出日期: 2022-04-28

Thrip Infestation on Sweet Peppers in Fujian and Pathogenicity of Lecanicillium lecanii against Dominant Species of Thrips

1.
Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Engineering Research Center for Green Pest Management, Fuzhou, Fujian　350013, China
2.
Zhangzhou Institute of Agricultural Science, Zhangzhou, Fujian　363005, China
3.
Research and Development Centre of Zhangzhou National Agricultural Science and Technology Zone, Zhangzhou, Fujian　363204, China

摘要

摘要: 目的明确福建甜椒蓟马发生情况及蜡蚧轮枝菌对蓟马优势种的生防潜力。方法以主产地漳州的甜椒种植基地为观测点，调查3种甜椒品种上蓟马的种类和数量，测定蜡蚧轮枝菌LL-01对甜椒蓟马优势种的致病力。结果甜椒路帅品种和布兰妮品种上蓟马数量近似且均显著高于特雅斯品种；甜椒上有花蓟马、黄胸蓟马和棕榈蓟马3种蓟马，其中花蓟马的相对丰度高达91.31%～94.93%。菌株LL-01侵染花蓟马7 d后的LC₅₀达3.47×10⁵孢子·mL⁻¹，1×10⁷～1×10⁸孢子·mL⁻¹处理的累计致死率为78.16%～87.36%，LT₅₀为3.73～4.24 d。结论蓟马对不同甜椒品种的嗜好性不同，花蓟马为优势种；蜡蚧轮枝菌LL-01对花蓟马的致病力强，可用于该虫的生物防治。
- 蓟马 /
- 甜椒 /
- 嗜好性 /
- 优势种 /
- 蜡蚧轮枝菌 /
- 致病力
Abstract: Objective Thrips infestation on sweet peppers in Fujian province was surveyed, and potential of using Lecanicillium lecanii as a means of biological control against the dominant species of thrips was explored. Methods Species and numbers of thrips that infested varieties of sweet peppers at vegetable farms in Zhangzhou, Fujian were surveyed. Pathogenicity of L. lecanii LL-01 on the dominant species of thrips was determined in laboratory. Results The total thrip populations on Lushuai and Bulanni sweet peppers were similar but significantly higher than that on Teyasi. The thrips found were largely Frankliniella intonsa, Thrips hawaiiensis, and T. palmi, and among them, F. intonsa being the most abundant, accounting for 91.31-94.93%. The LC₅₀ of L. lecanii LL-01 was 3.47×10⁵ conidia·mL⁻¹ on F. intonsa 7 d after an inoculated infection. The cumulative lethality was 78.16-87.36% under treatments of 1×10⁷ to 1×10⁸ conidia·mL⁻¹ to reach an LT₅₀ in 3.73-4.24 d. Conclusion Thrips had varied preferred host plants.F. intonsa was the dominant species that infested the sweet pepper varieties in the province. Since L. lecanii LL-01 was found strongly pathogenic to F. intonsa, it could be applied as a biological agent to deter wide-spread infestation.
- Thrips /
- sweet pepper /
- host preference /
- dominant species /
- Lecanicillium lecanii /
- pathogenicity

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图 1 甜椒蓟马对3种甜椒的嗜好性

注：①A，甜椒蓟马为害状；B，蓟马在3种甜椒上的虫口总量。②不同小写字母表示处理间差异显著（P<0.05）。

Figure 1. Preference of thrips on 3 varieties of sweet peppers

Note: ①A: Symptom of thrip infestation on sweet peppers; B: Total population of thrips on 3 varieties of sweet peppers. ②Data with different lowercase letters indicate significant difference (P＜0.05) between treatments.

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图 2 甜椒上的3种蓟马

注：A. 花蓟马；B. 黄胸蓟马；C. 棕榈蓟马。

Figure 2. Three species of thrips infested sweet peppers

Note: A: F. intonsa; B: T. hawaiiensis; C: T. palmi.

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图 3 菌株LL-01对花蓟马的侵染（A）及致死率（B）

Figure 3. Infectivity (A) and lethality (B) of LL-01 on F. intonsa

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表 1 3种甜椒上蓟马的种群数量和相对丰度

Table 1. Population and relative abundance of thrips on 3 varieties of sweet peppers

蓟马种类 Thrip species	特雅斯 Teyasi		路帅 Lushuai		布兰妮 Bulanni
蓟马种类 Thrip species	种群数量 Population number （P）	相对丰度 Relative abundance （R）	种群数量 Population number （P）	相对丰度 Relative abundance （R）	种群数量 Population number （P）	相对丰度 Relative abundance （R）
花蓟马 Frankliniella intonsa	730.67±57.18 a	91.31±1.83 a	1725.00±354.04 a	94.60±0.84 a	1538.00±61.65 a	94.93±0.72 a
黄胸蓟马 Thrips hawaiiensis	60.00±13.75 b	7.46±1.33 b	81.33±16.26 b	4.53±0.91 b	65.00±7.55 b	4.03±0.61 b
棕榈蓟马 Thrips palmi	10.00±4.58 b	1.23±0.51 c	16.00±4.58 b	0.87±0.08 c	17.00±9.17 b	1.05±0.55 c
注：P 表示Population；R表示Relative abundance（%）；同列数据后不同字母表示处理间差异显著。 Note: P means Population; R means Relative abundance (%); Different lowercase letters in the same column indicates significantly different.

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表 2 菌株LL-01对花蓟马的LC₅₀

Table 2. LC₅₀ of LL-01 on F. intonsa

时间 Time/d	回归方程 Virulence regression equation	致死中浓度 LC_50/ (conidia·mL⁻¹)	95%置信区间 95% Confidence interval/ (conidia·mL⁻¹)
4	y=1.1428+0.5562x	8.60×10⁶	4.55×10⁶～13.39×10⁷
5	y=1.9996+0.5157x	6.58×10⁵	3.55×10⁵～1.18×10⁶
6	y=2.2679+0.4931x	3.47×10⁵	1.70×10⁵～6.46×10⁶
7	y=2.2679+0.4931x	3.47×10⁵	1.70×10⁵～6.46×10⁶

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表 3 菌株LL-01对花蓟马的LT₅₀

Table 3. LT₅₀ of LL-01 on F. intonsa

孢子含量 Spore content/ (conidia·mL^-1)	回归方程 Virulence regression equation	致死中时 LT₅₀/ d	95%置信区间 95% Confidence interval/d
1×10⁶	y=0.7912+5.8882x	5.19	4.16～6.47
1×10⁷	y=0.8772+6.5684x	4.24	3.50～5.14
1×10⁸	y=0.9855+7.0199x	3.73	3.06～4.54

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参考文献(15)

[1]	陈锦伟. 诏安县甜椒产业化发展对策研究[D]. 福州: 福建农林大学, 2014 CHEN J W. Study on Development Strategies for Sweet Pepper Industrialization in Zhao'an County[D]. Fuzhou: Fujian Agriculture and Forestry University, 2014. (in Chinese)
[2]	漳州市统计局. 漳州统计年鉴2020(07农业)[DB/OL]. (2020-11-04) [2021-11-01]. http://www.zhangzhou.gov.cn/cms/html/zzsrmzf/2020-11-04/1634547002.html.
[3]	袁盛勇, 孔琼, 薛春丽, 等. 蜡蚧轮枝菌MZ041024菌株对棕榈蓟马的毒力测定 [J]. 华中农业大学学报, 2010, 29(5):560−563. YUAN S Y, KONG Q, XUE C L, et al. Measure of virulence of Verticillium lecanii MZ041024 to Thrips palmi karny [J]. Journal of Huazhong Agricultural University, 2010, 29(5): 560−563.(in Chinese)
[4]	袁盛勇, 孔琼, 张宏瑞, 等. 蜡蚧轮枝菌对西花蓟马成虫和若虫的毒力测定 [J]. 北方园艺, 2011(10):131−133. YUAN S Y, KONG Q, ZHANG H R, et al. Detection on the virulence of Verticillium lecanii against adult and nymph of frankliniellao ccidentalis(pergande) [J]. Northern Horticulture, 2011(10): 131−133.(in Chinese)
[5]	FLORES-MOCTEZUMA H E, LUNA-GUTIÉRREZ C, CASTREJÓN-AYALA F. Effectiveness of Lecanicillium lecanii for control of Thrips tabaci Lindeman on onion crop [J]. Journal of Chemical, Biological and Physical Sciences, 2014(4): 5,95.
[6]	余德亿, 黄鹏, 姚锦爱, 等. 蜡蚧轮枝菌V3450菌株对榕管蓟马及斯氏钝绥螨的毒力比较 [J]. 西北农林科技大学学报(自然科学版), 2015, 43(8):133−139. YU D Y, HUANG P, YAO J A, et al. Toxicity comparison of Lecanicillium lecanii V3450 strain to Gynaikothrips uzeli and Amblyseius (Typhlodromips) swirskii [J]. Journal of Northwest A& F University (Natural Science Edition), 2015, 43(8): 133−139.(in Chinese)
[7]	黄鹏, 姚锦爱, 余德亿. 一株蜡蚧轮枝菌LL-01及其生防应用: CN113046249A[P]. 2021-06-29.
[8]	谢婷, 景亮亮, 张晓霞, 等. 八种常用农药与蜡蚧轮枝菌 JMC-01的相容性及对烟粉虱若虫的毒力测定 [J]. 应用昆虫学报, 2020, 57(3):682−689. XIE T, JING L L, ZHANG X X, et al. Compatibility of 8 common pesticides with Lecanicillium lecanii JMC-01 and the toxicity of different pesticide-L. lecanii combinations toxicity to Bemisia tabaci nymphs [J]. Chinese Journal of Applied Entomology, 2020, 57(3): 682−689.(in Chinese)
[9]	韩运发, 徐祖荫. 中国农作物蓟马[M]. 北京: 中国农业出版社, 1982: 211−215.
[10]	余德亿, 黄鹏, 姚锦爱, 等. 盆栽榕树蓟马种类及优势种榕管蓟马对寄主植物的致害性 [J]. 昆虫学报, 2012, 55(7):832−840. YU D Y, HUANG P, YAO J A, et al. Species of Thrips on potted Ficus and the degree of damage to different host plants by the dominant species Gynaikothrips uzeli (Thysanoptera: Thripidae) [J]. Science China Earth Sciences, 2012, 55(7): 832−840.(in Chinese)
[11]	黄亚冰, 赵琳. 不同类型杀虫剂单独及混配防治大棚甜椒蓟马田间药效试验 [J]. 长江蔬菜, 2008(24):51−52. HUANG Y B, ZHAO L. Control of Thrips in sweet pepper by different types of pesticide [J]. Journal of Changjiang Vegetables, 2008(24): 51−52.(in Chinese)
[12]	穆常青, 杨海霞, 谷培云, 等. 9种药剂对温室彩椒西花蓟马的田间防效评价 [J]. 中国蔬菜, 2014(10):37−39. doi: 10.3969/j.issn.1000-6346.2014.10.008 MU C Q, YANG H X, GU P Y, et al. Field control efficiency of 9 insecticides against Frankliniella occidentalis (pargande) of greenhouse color sweet pepper [J]. China Vegetables, 2014(10): 37−39.(in Chinese) doi: 10.3969/j.issn.1000-6346.2014.10.008
[13]	张安盛, 于毅, 庄乾营, 等. 棕榈蓟马成虫在日光温室菜椒上的种群动态和空间分布 [J]. 植物保护学报, 2014, 41(2):210−215. ZHANG A S, YU Y, ZHUANG Q Y, et al. Population dynamics and spatial distribution of Thrips palmi on sweet pepper in solar greenhouses [J]. Journal of Plant Protection, 2014, 41(2): 210−215.(in Chinese)
[14]	姜灵. 蜡蚧轮枝菌的生物学特性及与常用杀虫剂对烟粉虱的协同作用[D]. 银川: 宁夏大学, 2018. JIANG L. Biological Characterizatics of Lecanicillium Lecanii and synergistic with insecticides to Bemisia Tabaci[D]. Yinchuan: Ningxia University, 2018. (in chinese)
[15]	黄鹏, 姚锦爱, 余德亿, 等. 虫生真菌BB-T02分离鉴定及其对两种蓟马的致病力 [J]. 中国生物防治学报, 2020, 36(6):929−937. HUANG P, YAO J A, YU D Y, et al. Identification and Pathogenicity of Entomopathogenic Fungi BB-T02 against Two Thrip Species [J]. Chinese Journal of Biological Control, 2020, 36(6): 929−937.(in Chinese)