高表没食子儿茶素-3-O-(3-O-甲基）没食子酸酯茶树种质资源的筛选与评价

陈潇敏; 金珊; 吴文晞; 邵淑贤; 王淑燕; 王鹏杰; 赵峰; 叶乃兴

doi:10.19303/j.issn.1008-0384.2022.004.007

高表没食子儿茶素-3-O-(3-O-甲基）没食子酸酯茶树种质资源的筛选与评价

doi: 10.19303/j.issn.1008-0384.2022.004.007

陈潇敏^1,,
金珊¹,
吴文晞²,
邵淑贤¹,
王淑燕¹,
王鹏杰¹,
赵峰^3, ,,
叶乃兴^1, ,

1.
福建农林大学园艺学院/茶学福建省高校重点实验室，福建福州　350002
2.
福建恒正检测技术有限公司，福建福州　350100
3.
福建中医药大学药学院，福建福州　350122

基金项目: 安溪县农业农村局重大科技创新专项（AX2021001）；福建中医药大学引进人才科研启动项目(2801/701190097)；福建农林大学优秀硕士学位论文资助基金（1122YS01003）

详细信息

作者简介:
陈潇敏（1996−），女，硕士研究生，主要从事茶树栽培育种与品质化学研究（Email：1936869612@qq.com）

通讯作者:
赵峰（1983−），男，硕士生导师，副教授，主要从事茶叶品质化学研究（E-mail：zhaofeng0591@fjtcm.edu.cn）

叶乃兴（1963−），男，硕士生导师，教授，主要从事茶树栽培育种与茶叶品质化学研究（E-mail：ynxtea@126.com）

中图分类号: S 571.1
计量
- 文章访问数: 685
- HTML全文浏览量: 140
- PDF下载量: 29
- 被引次数: 0
出版历程
- 收稿日期: 2021-12-26
- 修回日期: 2022-03-20
- 网络出版日期: 2022-04-24
- 刊出日期: 2022-04-28

Screening and Evaluation of Tea Germplasms Containing High-EGCG3″Me

1.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, Fujian　350002, China
2.
Hengzheng Testing Technology Company, Fuzhou, Fujian　350100, China
3.
School of Pharmacy, Fujian University of Chinese Medicine, Fuzhou, Fujian　350122, China

摘要

摘要: 目的探明福建野生茶树种质资源儿茶素组分特征，为福建野生茶树种质资源的开发利用和茶树特异品种筛选提供科学依据。方法以43份福建野生茶树种质资源和8个栽培品种为供试材料，采用高效液相色谱（HPLC）测定其儿茶素组分含量，并进行描述统计分析、正交偏最小二乘判别分析、聚类热图分析及特异性种质资源筛选。结果 43份福建野生茶树种质资源均检测出表没食子儿茶素-3-O-(3-O-甲基）没食子酸酯（EGCG3"Me），其中以云霄6号（YX06）含量最高，为33.65 mg·g⁻¹。43份福建野生茶树种质资源的特征物质为表没食子儿茶素-3-O-(3-O-甲基）没食子酸酯（EGCG3"Me）及表没食子儿茶素没食子酸酯（EGCG），且二者含量极显著高于8个栽培品种。聚类分析表明12份野生茶树种质资源与8个栽培品种聚为一类，其中以诏安和蕉城地区居多，其表儿茶素（EC）、表没食子儿茶素（EGC）和儿茶素（C）含量较高；其余31份野生茶树种质资源聚为一类，其表没食子儿茶素-3-O-(3-O-甲基）没食子酸酯（EGCG3"Me）、表儿茶素没食子酸酯（ECG）和表没食子儿茶素没食子酸酯（EGCG）含量较高。筛选获得高EGCG3"Me茶树资源（＞20 mg·g⁻¹）8份，高EGCG茶树资源（＞150 mg·g⁻¹）9份和高儿茶素茶树资源（＞200 mg·g⁻¹）18份。结论对福建野生茶树种质儿茶素组分进行测定及系统分析，发现其富含EGCG3"Me，并筛选出大量特异种质。
- 茶树 /
- 种质资源 /
- 儿茶素 /
- 表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯
Abstract: Objective Catechin composition of tea germplasms in the wild in Fujian was analyzed to aid organizing and utilizing the natural resources containing the highly desirable functional ingredients in tea. Methods Forty-three wild tea germplasms in Fujian and 8 common cultivars were collected for chemical analysis by HPLC. Descriptive statistical, orthogonal partial least squares discriminant, and cluster heat map analyses were conducted for evaluating and screening the candidates. Results EGCG3″Me was detected in all 43 specimens with the highest content of 33.65 mg·g⁻¹ found in YX06. Aside from EGCG3″Me, EGCG was also characteristically in significantly greater quantities in the wild tea germplasms than the 8 cultivated varieties. The cluster analysis grouped 12 wild germplasms, mostly from Zhao'an and Jiaocheng areas, along with the 8 cultivated varieties that had high contents of EC, EGC, and catechin (C). The remaining 31 wild tea germplasms were clustered into a group that had high contents of EGCG3″Me, ECG, and EGCG. Of all the 43 wild tea germplasms, 8 had high EGCG3″Me content (i.e., greater than 20mg·g⁻¹), 9 high EGCG (i.e., greater than 150 mg·g⁻¹), and 18 high C (i.e., greater than 200 mg·g⁻¹). Conclusion The tea germplasms collected in the wild in the province were rich in EGCG3″Me, which could be specific cultivars for target breeding of tea.
- Camellia sinensis /
- wild germplasms /
- catechin /
- EGCG3″Me

HTML全文

图 1 标准品及参试样品的色谱

注：（1）A：质量浓度为400 μg·mL混合标准工作液；B.：大田4号（DT04）；C：云霄6号（YX06）；D：漳平1号（ZP01）（2）峰号和组分依次对应为：1. GC，2. EGC，3. C，4. EGCG，5. EC，6. GCG，7. EGCG3′Me，8. ECG，9. CG。

Figure 1. Chromatograms of standards and test samples

Note: （1）A: STD400; B: DT04; C: YX06; D: ZP01. (2) Peak number and respective component are 1 for GC, 2 for EGC, 3 for C, 4 for EGCG, 5 for EC, 6 for GCG, 7 for EGCG3″Me, 8 for ECG, and 9 for CG.

下载: 全尺寸图片幻灯片

图 2 供试资源儿茶素组分含量

注：**表示相关性极显著（P＜0.01）。

Figure 2. Content of tea germplasms in catechin components

Note: ** represents highly significant correlation (P＜0.01).

下载: 全尺寸图片幻灯片

图 3 43份福建野生茶树资源及8个栽培品种儿茶素组分的OPLS-DA分析

Figure 3. OPLS-DA statistical analysis on catechins in 43 wild tea germplasms in Fujian and 8 cultivated varieties

下载: 全尺寸图片幻灯片

图 4 43份福建野生茶树资源及8个栽培品种儿茶素组分聚类热图可视化分析

Figure 4. Heatmap analysis on catechins in 43 wild tea germplasms in Fujian and 8 cultivated varieties

下载: 全尺寸图片幻灯片

表 1 供试茶树种质资源信息

Table 1. Information on tea germplasms

种质资源类型 Type of germplasm resources	来源 Source site	种质数 Germplasm number/份	茶树种质资源样品 Samples of tea plant germplasm resources
福建野生茶树资源 Wild tea resources of Fujian	蕉城 Jiaocheng	3	蕉城1号—蕉城3号（JC01—JC03）
	云霄 Yunxiao	6	云霄1号—云霄6号（YX01—YX06）
	大田 Datian	6	大田1号—大田6号（DT01—DT06）
	尤溪 Youxi	6	尤溪汤川1号—尤溪汤川6号（TC01—TC06)
	安溪 Anxi	1	安溪苦茶（AX01）
	诏安 Zhaoan	10	诏安1号—诏安10号（ZA01—ZA10)
	漳平 Zhangping	11	漳平1号—漳平11号（ZP01—ZP11）
栽培品种 Cultivated variety	武夷学院茶树种质资源圃 Tea germplasm resource nursery of Wuyi University	8	福鼎大白茶（FD）、福云6号（FY6）、黄旦（HD）、金观音（JGY）、黄金芽（HJY）、龙井43（LJ43）、白叶1号（BY1）、嘉茗1号（JM1）

下载: 导出CSV

表 2 43份福建野生茶树资源及8个栽培品种儿茶素组分含量

Table 2. Catechins in 43 wild tea germplasms in Fujian and 8 cultivated varieties （单位：mg·g⁻¹）

编号 Number	简单儿茶素 Simple catechins				酯型儿茶素 Ester catechins					总量 Total
编号 Number	EC	C	EGC	GC	EGCG3″Me	ECG	EGCG	GCG	CG	总量 Total
JC01	10.33±0.53	1.04±0.05	42.91±2.05	—	9.77±1.04	15.13±0.70	68.36±2.94	—	—	147.54
JC02	8.08±0.62	0.75±0.19	41.18±0.50	—	15.05±1.77	13.76±1.04	76.00±5.38	—	—	154.82
JC03	6.90±0.65	0.58±0.1	22.76±1.44	—	8.93±0.84	12.96±0.91	53.27±3.89	—	—	105.40
YX01	2.12±0.39	0.91±0.13	8.46±0.76	—	4.96±0.49	14.06±1.20	58.38±5.58	—	—	88.89
YX02	2.72±0.26	1.15±0.14	9.12±0.70-	—	5.00±0.67	11.43±1.28	65.50±7.14	—	—	94.92
YX03	8.97±0.26	1.49±0.03	31.23±0.57	—	9.57±1.03	21.83±0.57	111.20±2.70	—	—	174.72
YX04	4.90±0.17	10.33±0.33	7.91±0.11	—	11.08±0.35	20.28±0.72	90.32±3.00	—	—	144.82
YX05	10.61±0.47	1.69±0.11	31.09±0.65	—	25.54±0.44	26.88±0.62	125.53±2.83	—	—	221.34
YX06	10.55±0.95	1.77±0.38	41.17±2.65	—	33.65±1.70	30.87±0.47	142.27±2.32	—	—	260.28
DT01	0.84±0.12	—	6.06±1.52	—	3.54±1.01	13.93±3.97	72.56±15.87	—	—	96.93
DT02	—	1.33±0.47	4.02±0.52	—	6.30±0.92	13.01±1.68	74.63±12.11	—	—	99.29
DT03	5.50±0.41	2.23±0.06	23.70±1.97	—	12.84±0.31	24.00±1.01	160.70±5.39	—	—	228.97
DT04	5.69±0.34	2.40±0.22	19.47±1.08	—	14.13±0.52	25.58±1.35	179.35±8.97	—	—	246.62
DT05	5.24±0.11	—	28.43±0.63	—	16.45±0.71	16.55±0.42	148.72±3.84	—	—	215.39
DT06	6.27±0.23	1.49±0.06	28.21±0.93	—	5.39±1.45	22.21±0.79	174.76±6.47	—	—	238.33
TC01	5.03±0.26	4.28±0.40	14.17±0.62	4.61±0.36	7.91±0.75	24.75±1.55	116.26±10.32	—	—	177.01
TC02	13.98±0.60	6.77±0.51	30.18±2.31	9.08±0.68	7.33±0.30	37.53±1.98	132.71±8.28	0.49±0.06	—	238.07
TC03	5.29±0.04	4.82±0.31	2.49±0.34	8.74±0.40	18.11±0.50	35.58±0.98	140.70±4.19	—	—	215.73
TC04	4.01±0.87	3.58±0.18	10.97±0.36	5.62±0.27	20.70±1.95	20.64±0.96	119.47±7.15	—	—	184.99
TC05	9.11±0.14	5.24±0.09	26.77±0.36	7.98±0.18	4.23±0.06	29.51±2.69	159.92±0.85	0.58±0.05	—	243.34
TC06	10.18±0.27	6.09±0.24	27.25±0.74	8.40±0.21	5.59±0.20	29.02±0.62	150.96±3.55	0.84±0.01	—	238.33
AX01	8.37±0.09	1.40±0.03	27.27±0.23	—	13.01±0.88	23.42±0.50	141.10±5.44	—	—	214.57
ZA01	1.63±0.40	4.76±0.33	5.50±0.32	1.37±0.29	14.34±1.33	21.01±1.67	109.04±8.67	—	—	157.65
ZA02	2.54±0.20	3.32±0.30	8.85±0.60	—	7.16±0.69	16.80±0.76	83.23±5.88	—	—	121.90
ZA03	2.10±0.60	2.18±0.33	7.65±0.78	—	7.96±1.25	11.27±1.25	84.86±10.54	—	—	116.02
ZA04	2.02±0.90	2.10±0.62	7.31±2.26	—	12.01±4.49	10.72±3.40	70.31±18.71	—	—	104.47
ZA05	0.76±0.31	1.80±0.78	1.95±0.99	—	2.17±1.30	8.23±4.10	23.32±12.15	—	—	38.23
ZA06	1.52±0.84	2.87±1.65	4.43±2.19	—	5.28±4.02	10.51±4.92	33.64±0.52	—	—	58.25
ZA07	3.85±0.35	2.58±0.49	10.07±0.86	—	9.54±0.61	21.13±1.14	112.40±9.71	—	—	159.57
ZA08	2.44±0.30	2.22±0.29	8.36±0.86	—	21.03±3.22	27.15±3.69	127.84±17.55	—	—	189.04
ZA09	2.07±0.34	—	10.66±2.41	—	8.78±1.48	12.11±2.03	96.41±16.49	—	—	130.03
ZA10	4.16±0.81	0.92±0.21	25.28±3.69	—	12.00±4.61	20.77±5.09	171.15±16.58	—	—	241.99
ZP01	4.36±0.98	2.21±0.91	21.02±5.08	—	20.66±3.93	24.9±1.05	186.87±3.11	—	—	260.02
ZP02	6.23±1.50	1.96±0.60	25.43±5.12	—	13.19±5.88	33.84±5.28	168.17±1.65	—	—	248.82
ZP03	7.55±0.96	0.91±0.27	24.42±1.24	—	14.78±1.61	22.01±3.17	80.89±0.20	—	—	150.56
ZP04	4.82±0.61	0.77±0.14	20.55±1.29	—	6.19±0.45	17.36±1.26	145.36±13.28	—	—	195.05
ZP05	4.93±0.87	1.95±0.20	18.01±2.28	—	19.42±2.89	33.30±4.43	180.00±1.31	—	—	257.61
ZP06	4.98±0.32	1.17±0.09	26.17±2.63	—	21.08±1.44	19.24±1.44	114.62±11.29	—	—	187.26
ZP07	6.08±0.24	1.51±0.15	26.18±1.75	—	20.98±0.43	24.89±0.31	135.34±1.42	—	—	214.98
ZP08	5.90±0.16	1.23±0.09	22.20±1.15	—	15.39±0.17	23.62±0.43	134.92±1.67	—	—	203.26
ZP09	3.37±0.31	2.27±0.62	9.88±1.95	—	28.18±0.73	29.07±0.57	114.05±2.98	—	—	186.82
ZP10	2.97±0.25	1.88±0.05	15.05±1.20	—	19.71±1.60	16.87±1.40	141.88±12.84	—	—	198.36
ZP11	19.35±0.03	3.20±0.07	40.95±1.12	—	11.25±0.06	38.33±0.18	102.64±0.37	—	—	215.72
FD	7.51±0.69	1.13±0.11	17.84±1.44	—	—	14.12±0.80	61.36±5.69	—	—	101.96
FY6	13.08±0.34	1.48±0.02	32.42±0.89	3.21±0.03	—	27.09±1.04	78.02±2.54	—	—	155.30
JGY	8.78±0.41	2.01±0.64	28.79±5.02	—	1.55±0.11	17.44±0.19	94.31±1.52	—	—	152.88
HD	9.08±0.45	1.74±0.26	24.01±2.39	—	1.65±0.03	20.75±0.63	73.60±1.95	—	—	130.83
HJY	12.65±0.58	2.08±0.16	29.46±1.39	—	—	19.87±0.78	57.01±1.90	—	—	121.07
LJ43	9.24±0.27	—	26.68±1.87	—	—	16.56±0.55	63.16±2.00	—	—	115.64
BY1	8.46±0.12	1.39±0.14	49.42±2.93	—	—	9.10±0.41	59.48±3.13	—	—	127.85
JM1	9.46±0.63	1.56±0.03	38.96±1.42	—	—	13.11±0.93	67.73±4.41	—	—	130.82
注：“—”表示未检出。 Note: “—” indicates not detected.

下载: 导出CSV

表 3 福建野生茶树特异资源的筛选

Table 3. Selection of tea germplasms from wild resources found in Fujian

资源类型　　　　 Gemplasm type	资源名称 Gemplasm name
高儿茶素 High catechin （＞200 mg·g⁻¹）	YX06(260.28)、ZP01(260.02)、ZP05(257.51)、ZP02(248.82)、DT04(246.62)、TC05(243.34)
	ZA10(241.99)、TC06(238.33)、DT06(238.33)、TC02(238.07)、DT03(228.97)、YX05(221.34)
	TC03(215.73)、ZP11(215.72)、DT05(215.39)、ZP07(214.98)、AX01(214.57)、ZP08(203.26)
高EGCG High EGCG （＞150 mg·g^-1）	ZP01(186.87)、ZP05(180.00)、DT04(179.35)、DT06(174.76)、ZA10(171.15)、ZP02(168.17) 、DT03(160.70)、TC05(159.92)、TC06(150.96)
高EGCG3"Me High EGCG3''Me（＞20 mg·g⁻¹）	YX06(33.65)、ZP09(28.18)、YX05(25.54)、ZP06(21.08)、ZA08(21.03)、ZP07(20.98)
高EGCG3"Me High EGCG3''Me（＞20 mg·g⁻¹）	TC04(20.70)、ZP01(20.66)

下载: 导出CSV

参考文献(26)

[1]	MOLDOVEANU S C, ODEN R. Antioxidant character and levels of polyphenols in several tea samples [J]. ACS Omega, 2021, 6(15): 9982−9988. doi: 10.1021/acsomega.0c05818
[2]	ZHANG Y, WANG S X, MA J W, et al. EGCG inhibits properties of glioma stem-like cells and synergizes with temozolomide through downregulation of P-glycoprotein inhibition [J]. Journal of Neuro-Oncology, 2015, 121(1): 41−52. doi: 10.1007/s11060-014-1604-1
[3]	叶乃兴. 茶学概论[M]. 2版. 北京: 中国农业出版社, 2021.
[4]	王蔚, 郭雅玲. 茶功能性成分对肺癌作用机制的研究进展 [J]. 中草药, 2017, 48(17):3654−3661. doi: 10.7501/j.issn.0253-2670.2017.17.032 WANG W, GUO Y L. Research progress in mechanisms of functional components in tea on lung cancer [J]. Chinese Traditional and Herbal Drugs, 2017, 48(17): 3654−3661.(in Chinese) doi: 10.7501/j.issn.0253-2670.2017.17.032
[5]	李银花, 张盛, 黄建安, 等. 茶叶中EGCG3 ″Me和EGCG4″Me的分离制备与鉴定 [J]. 茶叶科学, 2012, 32(4):313−318. doi: 10.3969/j.issn.1000-369X.2012.04.005 LI Y H, ZHANG S, HUANG J A, et al. Isolation and identification of EGCG3″Me and EGCG4″Me from tea [J]. Journal of Tea Science, 2012, 32(4): 313−318.(in Chinese) doi: 10.3969/j.issn.1000-369X.2012.04.005
[6]	JIN J Q, JIANG C K, YAO M Z, et al. Baiyacha, a wild tea plant naturally occurring high contents of theacrine and 3″-methyl-epigallocatechin gallate from Fujian, China [J]. Scientific Reports, 2020, 10(1): 1−9. doi: 10.1038/s41598-019-56847-4
[7]	林金科, 郑金贵, 陈荣冰, 等. 高EGCG含量的特异茶树种质资源的筛选与研究 [J]. 作物学报, 2005, 31(11):127−133. LIN J K, ZHENG J G, CHEN R B, et al. Screening specific tea plant germplasm resources[Camellia sinensis(L. ) O. Kuntze]with high EGCG content [J]. Acta Agronomica Sinica, 2005, 31(11): 127−133.(in Chinese)
[8]	唐琴, 孙威江, 陈志丹, 等. 尤溪苦茶资源苦涩味物质测定与分析 [J]. 食品科学, 2019, 40(18):242−247. doi: 10.7506/spkx1002-6630-20181030-362 TANG Q, SUN W J, CHEN Z D, et al. Determination and analysis of bitter and astringent substances in Youxi bitter tea resources [J]. Food Science, 2019, 40(18): 242−247.(in Chinese) doi: 10.7506/spkx1002-6630-20181030-362
[9]	柳敏, 饶国武, 华允芬. EGCG衍生物合成及药理活性研究进展 [J]. 茶叶科学, 2016, 36(2):119−130. doi: 10.3969/j.issn.1000-369X.2016.02.002 LIU M, RAO G W, HUA Y F. Research advance in synthesis and pharmacological effects of EGCG derivatives [J]. Journal of Tea Science, 2016, 36(2): 119−130.(in Chinese) doi: 10.3969/j.issn.1000-369X.2016.02.002
[10]	FUJIMURA Y, FUJINO K, YOSHIMOTO T, et al. Eriodictyol-amplified 67-kDa laminin receptor signaling potentiates the antiallergic effect of O-methylated catechin [J]. Journal of Natural Products, 2021, 84(6): 1823−1830. doi: 10.1021/acs.jnatprod.1c00337
[11]	谢凤, 孙威江, 邓婷婷. 茶树表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯研究进展 [J]. 天然产物研究与开发, 2019, 31(7):1291−1297. XIE F, SUN W J, DENG T T. Research advance of epigallocatechin-3-O-(3-O-methyl)-gallate [J]. Natural Product Research and Development, 2019, 31(7): 1291−1297.(in Chinese)
[12]	雷志伟, 李露露, 郭灿, 等. 儿茶素糖基化修饰研究进展 [J]. 中草药, 2019, 50(21):5362−5372. doi: 10.7501/j.issn.0253-2670.2019.21.033 LEI Z W, LI L L, GUO C, et al. Research progress on glycosidation modification of catechins [J]. Chinese Traditional and Herbal Drugs, 2019, 50(21): 5362−5372.(in Chinese) doi: 10.7501/j.issn.0253-2670.2019.21.033
[13]	孙业良. 茶叶中甲基EGCG的研究[D]. 南京: 南京农业大学, 2009. SUN Y L. Study on O-methylated egcg in tea[D]. Nanjing: Nanjing Agricultural University, 2009. (in Chinese)
[14]	汪毅. 茶叶中甲基化EGCG的调查研究[D]. 重庆: 西南大学, 2006. WANG Y. Studies on O-methylated epigallocatechin-3-O-gallate in tea[D]. Chongqing: Southwest University, 2006. (in Chinese)
[15]	LV H P, YANG T, MA C Y, et al. Analysis of naturally occurring 3″-Methyl-epigallocatechin gallate in 71 major tea cultivars grown in China and its processing characteristics [J]. Journal of Functional Foods, 2014, 7: 727−736. doi: 10.1016/j.jff.2013.12.009
[16]	陈潇敏, 章进汕, 金珊, 等. 福建大田县茶树品种资源生化成分特征分析与评价 [J]. 南方农业学报, 2022, 52(4):1050−1057. CHEN X M, ZHANG J S, JIN S, et al. Analysis on biochemical components of local tea variety resources in Datian, Fujian [J]. Journal of Southern Agriculture, 2022, 52(4): 1050−1057.(in Chinese)
[17]	陈潇敏, 赵峰, 金珊, 等. 福建云霄地方茶树品种资源生化成分特征分析与评价 [J]. 西北植物学报, 2022, 42(1):127−137. CHEN X M, ZHAO F, JIN S, et al. Analysis and evaluation on biochemical components of local tea variety resources in Yunxiao, Fujian [J]. Acta Botanica Boreali-Occidentalia Sinica, 2022, 42(1): 127−137.(in Chinese)
[18]	陈潇敏, 赵峰, 王淑燕, 等. 福建野生茶树资源嘌呤生物碱构成评价及特异资源筛选 [J]. 茶叶科学, 2022, 42(1):18−28. doi: 10.3969/j.issn.1000-369X.2022.01.002 CHEN X M, ZHAO F, WANG S Y, et al. Purine alkaloid evaluation and excellent resources screening of Fujian wild tea [J]. Journal of Tea Science, 2022, 42(1): 18−28.(in Chinese) doi: 10.3969/j.issn.1000-369X.2022.01.002
[19]	王丽丽, 陈键, 宋振硕, 等. 茶叶中没食子酸、儿茶素类和生物碱的HPLC检测方法研究 [J]. 福建农业学报, 2014, 29(10):987−994. doi: 10.3969/j.issn.1008-0384.2014.10.011 WANG L L, CHEN J, SONG Z S, et al. Simultaneous HPLC determination of Gallic acid, catechins and alkaloids in tea [J]. Fujian Journal of Agricultural Sciences, 2014, 29(10): 987−994.(in Chinese) doi: 10.3969/j.issn.1008-0384.2014.10.011
[20]	王新超, 陈亮, 杨亚军. 广西茶树资源生化成分多样性分析 [J]. 植物遗传资源学报, 2010, 11(3):309−314,319. WANG X C, CHEN L, YANG Y J. Biochemical diversity analysis of tea germplasms in Guangxi [J]. Journal of Plant Genetic Resources, 2010, 11(3): 309−314,319.(in Chinese)
[21]	NING Y, LING J Q, WU C D. Synergistic effects of tea catechin epigallocatechin gallate and antimycotics against oral Candida species [J]. Archives of Oral Biology, 2015, 60(10): 1565−1570. doi: 10.1016/j.archoralbio.2015.07.001
[22]	LI J H, CHEN S X, ZHU M Z, et al. Cluster analysis of the biochemical composition in 53 Sichuan EGCG3"Me tea resources [J]. IOP Conference Series:Materials Science and Engineering, 2017, 231: 012125. doi: 10.1088/1757-899X/231/1/012125
[23]	卢秋华, 陈志雄, 彭建肯, 等. 高甲基化儿茶素乌龙茶品种的筛选初探 [J]. 厦门科技, 2018(6):46−48. doi: 10.3969/j.issn.1007-1563.2018.06.013 LU Q H, CHEN Z X, PENG J K, et al. Screening of hypermethylated catechin oolong tea varieties [J]. Xiamen Science & Technology, 2018(6): 46−48.(in Chinese) doi: 10.3969/j.issn.1007-1563.2018.06.013
[24]	吕海鹏, 谭俊峰, 林智. 茶树种质资源EGCG3"Me含量及其变化规律研究 [J]. 茶叶科学, 2006, 26(4):310−314. doi: 10.3969/j.issn.1000-369X.2006.04.015 LU H P, TAN J F, LIN Z. Study on the content of EGCG3"me in different tea germplasms and its changes [J]. Journal of Tea Science, 2006, 26(4): 310−314.(in Chinese) doi: 10.3969/j.issn.1000-369X.2006.04.015
[25]	WEI C L, YANG H, WANG S B, et al. Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality [J]. PNAS, 2018, 115(18): 4151−4158.
[26]	JIN J Q, LIU Y F, MA C L, et al. A novel F3' 5' H allele with 14 bp deletion is associated with high catechin index trait of wild tea plants and has potential use in enhancing tea quality [J]. Journal of Agricultural and Food Chemistry, 2018, 66(40): 10470−10478.