牦牛妊娠期和产后乏情期卵巢蛋白组学分析

麻俊渊; 王进娥; 李洋; 胡凇茗; 陈舟; 李书元; 杨妍梅; 扎西英派; 霍生东

doi:10.19303/j.issn.1008-0384.2022.004.002

牦牛妊娠期和产后乏情期卵巢蛋白组学分析

doi: 10.19303/j.issn.1008-0384.2022.004.002

西北民族大学生命科学与工程学院，甘肃　兰州　730030

基金项目: 中央高校基本科研业务费专项资金项目（31920190204、31920200076）；甘肃省重点研发计划项目（17YF1NA067）；甘肃省教育厅优秀研究生“创新之星”项目（2021CXZX-683）

详细信息

作者简介:
麻俊渊（1997−），男，硕士研究生，研究方向：牦牛生殖调控（E-mail：Junyuanmaxbmd@163.com）

通讯作者:
霍生东（1979−），男，副教授，硕士生导师，研究方向：牦牛季节性发情、哺乳动物产后乏情等（E-mail：huoshd@xbmu.edu.cn）

中图分类号: S 857.2+1
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出版历程
- 收稿日期: 2021-11-14
- 修回日期: 2022-01-25
- 网络出版日期: 2022-06-19
- 刊出日期: 2022-04-28

Ovarian Proteomics of Yaks in Pregnancy and Postpartum Anestrus

Life Science and Engineering College, Northwest University for Nationalities, Lanzhou, Gansu　730030, China

摘要

摘要: 目的研究牦牛产后乏情期和妊娠期卵巢中差异蛋白的表达情况。方法选择青海省湟源县一户牧民自然放牧牛群中处于妊娠期和产后乏情期牦牛各3头为研究对象，采集卵巢组织，利用TMT定量蛋白质组学技术发掘不同时期卵巢差异表达蛋白。结果妊娠期和产后乏情期卵巢组织中差异表达蛋白有57个，其中乏情期卵巢组织中上调蛋白38个，下调蛋白19个；COG分析发现，差异表达蛋白功能集中于能量的生产与转化、蛋白质周转、氨基酸转运与代谢等过程；GO分析发现，差异表达蛋白集中于代谢、繁殖等生物过程，涉及细胞器成分等细胞组分，参与转运活性等分子功能；KEGG分析发现，差异表达蛋白共参与27条信号通路，其中，缓激肽（BK）、胆固醇侧链裂解酶（P450scc）参与卵巢类固醇激素生成通路，谷胱甘肽过氧化物酶1（GSH-P1）、谷胱甘肽S转移酶A2（GSTA2）、谷胱甘肽转移酶（GST）和天冬氨酸转氨酶（AST）参与氨基酸转运与蛋白质代谢通路，磷脂酰肌醇-3-激酶蛋白（PI3K）参与PI3K-Akt细胞凋亡信号通路。结论 BK、P450scc、GSH-P1、GSTA2、GST、AST和PI3K与牦牛乏情的产生密切相关。
- 妊娠期 /
- 产后乏情 /
- 蛋白质组学 /
- 卵巢 /
- 牦牛
Abstract: Objective Differentially expressed proteins (DEPs) in ovaries of yak s in pregnancy and postpartum anestrus were identified. Method Ovarian tissues of 3 randomly selected yaks each in pregnancy or postpartum anestrus from a natural grazing herd in Huangyuan County, Qinghai Province were sampled for DEP identification by the TMT method. Result Thirty-eight upregulated and 19 downregulated DEPs were found in the yaks. The COG analysis indicated the major functions of the proteins related to the production and transformation of energy, protein turnover, and amino acid transport and metabolism. The Go analysis showed that they were mainly annotated in the biological processes of metabolism and reproduction, associated with the cellular component including organelle cell components, and participated in molecular function including the molecular transport activity. Whereas KEGG revealed their involvements in 27 signaling pathways that included BK and P450scc in the ovarian steroid hormone production, GSH-P1, GSTA2, GST, and AST in the amino acid transport and protein metabolism, and PI3K in the PI3K-Akt apoptosis signaling. Conclusion The current study identified specific DEPs in yak ovary, i.e., BK, P450scc, GSH-P1, GSTA2, GST, AST, and PI3K, that were closely related to the onset of anestrus in the animal.
- Pregnancy /
- postpartum anestrus /
- proteomics /
- ovary /
- yak

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图 1 总蛋白主成分分析

Figure 1. Principal component analysis on all proteins

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图 2 差异表达蛋白热图

Figure 2. Heat map of DEPs

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图 3 差异表达蛋白COG分析

Figure 3. COG analysis on DEPs

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图 4 差异表达蛋白GO分析

Figure 4. GO analysis on DEPs

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图 5 差异表达蛋白KEGG分析

Figure 5. KEGG analysis on DEPs

下载: 全尺寸图片幻灯片

表 1 差异表达蛋白信息统计

Table 1. Statistical information on DEPs

序号 Number	标识　 ID	蛋白名称 Protein names	调节 Regulated
1	Q4U0F3	热休克 70 kDa 蛋白 1B (HSP70.2) Heat shock 70 kDa protein 1B (HSP70.2)	下调 Down
2	D4QBF3	血红蛋白β Hemoglobin beta	上调 Up
3	L8I5Y3	胶原结合蛋白 Collagen-binding protein	上调 Up
4	A0A5A9Q697	未知 Deleted	上调 Up
5	A0A5A9QF16	未知 Deleted	下调 Down
6	A0A5A9PW39	未知 Deleted	上调 Up
7	L8J5R1	谷胱甘肽 S-转移酶 A2（片段） Glutathione S-transferase A2 (Fragment)	上调 Up
8	A0A5A9PVT1	未知 Deleted	上调 Up
9	L8HPR0	缓激肽 Bradykinin	下调 Down
10	A0A5A9QYU8	未知 Deleted	下调 Down
11	L8ICZ3	天冬氨酸氨基转移酶 (EC 2.6.1.1) Aspartate aminotransferase (EC 2.6.1.1)	上调 Up
12	A0A5A9Q9K0	未知 Deleted	下调 Down
13	A0A5A9QIY6	未知 Deleted	上调 Up
14	L8HXR0	胆固醇侧链裂解酶，线粒体（EC 1.14.15.6） Cholesterol side-chain cleavage enzyme, mitochondrial (EC 1.14.15.6)	上调 Up
15	L8HVE1	EPM2A相互作用蛋1 EPM2A-interacting protein 1	上调 Up
16	L8INV1	肽基脯氨酰顺反异构酶 (EC 5.2.1.8) Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)	上调 Up
17	A0A5A9R284	未知 Deleted	上调 Up
18	A0A5A9QP43	未知 Deleted	上调 Up
19	A0A5A9Q6B8	未知 Deleted	上调 Up
20	A0A5A9QVH4	未知 Deleted	下调 Down
21	L8IKU4	D-3-磷酸甘油酸脱氢酶 (EC1.1.1.95) D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)	上调 Up
22	P00435	谷胱甘肽过氧化物酶 1 (GPx-1) (EC 1.11.1.9) Glutathione peroxidase 1 (GPx-1) (EC 1.11.1.9)	上调 Up
23	L8HR71	视网膜脱氢酶2 Retinal dehydrogenase 2	下调 Down
24	L8IBL3	氨基肽酶 (EC 3.4.11.-)（片段） Aminopeptidase (EC 3.4.11.-) (Fragment)	上调 Up
25	A0A5A9QJL1	未知 Deleted	上调 Up
26	A0A5A9Q7A9	未知 Deleted	上调 Up
27	A0A5A9Q4N6	未知 Deleted	下调 Down
28	L8HS12	未鉴定的蛋白质（片段） Uncharacterized protein (Fragment)	上调 Up
29	L8IZP3	肾上腺素，线粒体（片段） Adrenodoxin, mitochondrial (Fragment)	上调 Up
30	L8I6P6	非综合征性听力障碍蛋白5样蛋白 Non-syndromic hearing impairment protein 5-like protein	上调 Up
31	L8IL81	α-2-巨球蛋白受体相关蛋白 Alpha-2-macroglobulin receptor-associated protein	上调 Up
32	L8HM51	胰蛋白酶抑制剂（片段） Pancreatic trypsin inhibitor (Fragment)	上调 Up
33	L8HXY3	含有 Ig 样结构域的蛋白质（片段） Ig-like domain-containing protein (Fragment)	上调 Up
34	L8HVF6	硒蛋白 M Selenoprotein M	上调 Up
35	L8HX05	神经元特异性钙结合蛋白海马钙素 Neuron-specific calcium-binding protein hippocalcin	下调 Down
36	A0A5A9QIW4	未知 Deleted	下调 Down
37	L8HVH6	中性粒细胞明胶酶相关脂质运载蛋白 Neutrophil gelatinase-associated lipocalin	上调 Up
38	A0A5A9QMW0	未知 Deleted	上调 Up
39	L8IS41	E3 UFM1-蛋白连接酶 1 E3 UFM1-protein ligase 1	下调 Down
40	L8HY99	含三部分基序的蛋白质 3（片段） Tripartite motif-containing protein 3 (Fragment)	下调 Down
41	Q860K0	MHC I 类抗原（片段） MHC class I antigen (Fragment)	下调 Down
42	A0A5A9QEA2	未知 Deleted	上调 Up
43	L8HU38	含有 Ig 样结构域的蛋白质（片段） Ig-like domain-containing protein (Fragment)	下调 Down
44	L8I6S3	谷胱甘肽转移酶（EC 2.5.1.18）（片段） Glutathione transferase (EC 2.5.1.18) (Fragment)	下调 Down
45	A0A5A9QAJ2	未知 Deleted	上调 Up
46	L8HUU8	SRA 茎环相互作用 RNA 结合蛋白，线粒体 SRA stem-loop-interacting RNA-binding protein, mitochondrial	上调 Up
47	L8I2B7	碱性磷酸酶 (EC 3.1.3.1)（片段） Alkaline phosphatase (EC 3.1.3.1) (Fragment)	上调 Up
48	A0A5A9QZ00	未知 Deleted	下调 Down
49	A0A5A9Q3H5	未知 Deleted	下调 Down
50	A0A5A9R1T6	未知 Deleted	上调 Up
51	L8HU32	含有 Ig 样结构域的蛋白质（片段） Ig-like domain-containing protein (Fragment)	下调 Down
52	A0A5A9Q609	未知 Deleted	上调 Up
53	A0A5A9QMW5	未知 Deleted	上调 Up
54	L8IIL6	醌氧化还原酶 PIG3（片段） Quinone oxidoreductase PIG3 (Fragment)	上调 Up
55	L8IUD0	含有 EGF 样模块的粘蛋白样激素受体样 1（片段） EGF-like module-containing mucin-like hormone receptor-like 1 (Fragment)	上调 Up
56	L8IC02	ER 腔蛋白保留受体（片段） ER lumen protein-retaining receptor (Fragment)	上调 Up
57	L8HUM8	磷脂酰肌醇-4,5-二磷酸 3-激酶 (EC 2.7.1.153) Phosphatidylinositol-4,5-bisphosphate 3-kinase (EC 2.7.1.153)	下调 Down

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