盐渍化胁迫下接种不同丛枝菌根真菌对番茄耐盐性的影响

曹磊; 李逸雯; 凌康杰; 王婧绮; 鄢蕙; 牟丽娟; 江晋岚; 方芳; 刘瑞强

doi:10.19303/j.issn.1008-0384.2022.002.008

盐渍化胁迫下接种不同丛枝菌根真菌对番茄耐盐性的影响

doi: 10.19303/j.issn.1008-0384.2022.002.008

1.
浙江师范大学化学与生命科学学院，浙江　金华　321004
2.
东北林业大学林学院，黑龙江　哈尔滨　150040

基金项目: 国家自然科学基金项目（32001224）；浙江省公益技术应用研究计划项目（LGN19C140009）

详细信息

作者简介:
曹磊（1995−），男，硕士研究生，主要从事植物水分生理研究（E-mail：1648699734@qq.com）

通讯作者:
方芳（1969−），女，高级实验师，主要从事植物生理学研究（E-mail：sky85@zjnu.cn）

中图分类号: S 641.2
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-10
- 修回日期: 2022-02-10
- 刊出日期: 2022-02-25

Effects of Arbuscular Mycorrhizal Fungi Inoculation on Salt-tolerance of Tomato Plants

1.
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang　321004, China
2.
School of Forestry, Northeast Forestry University, Harbin, Heilongjiang　150040, China

摘要

摘要: 目的研究不同丛枝菌根真菌（AMF）对番茄植株生长和抗盐胁迫效应，探究筛选出能够延缓盐分对番茄生理活性的抑制，提升植株光合碳同化能力和耐盐性能力的最佳AMF真菌。方法针对土壤盐渍化对番茄的不良效应，通过土培法对番茄植株进行不同盐浓度（0、100 mmol·L⁻¹）处理，经初筛得到摩西（F.m）、根内（R.i）两种丛枝菌根真菌作为接种菌剂，从生理及光合2个角度探究对盐胁迫下接种不同AMF侵染对番茄的生长状况的影响。结果盐胁迫下番茄抗氧化酶系活性，包括超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、丙二醛（MDA）含量及脯氨酸（Pro）含量分别提高了47.4%、32.9%、35.7%、61.8%、6%。菌根侵染率、光合强度降低了27.8%和54.6%。接种AMF能有效增强宿主抗盐性，其中F.m接种后番茄的脯氨酸含量降低60.7%，降低幅度是接种R.i处理的2.2倍。F.m接种后番茄的净光合速率（P_n）、气孔导度（G_s）分别提高49.1%、35.4%，增幅是接种R.i处理的1.4倍。同时，接种AMF能减轻盐毒对光合关键酶的损伤，其中接种F.m后番茄RuBP羧化酶最大增长率可达31.2%，是接种R.i真菌处理的1.1倍。结论 AMF可延缓盐分对番茄生理活性的抑制，提升植株光合碳同化能力和耐盐性，其中接种摩西（F.m）真菌对植物促生作用显著优于根内（R.i）真菌。
- 丛枝菌根真菌 /
- 番茄 /
- 侵染率 /
- 生理活性 /
- 盐胁迫
Abstract: Objective Effect of introducing various arbuscular mycorrhizal fungi (AMF) in soil on the growth and salt-tolerance of tomato plants was studied. Method After a preliminary screening, two potentially applicable AMF, Moses (F.m) and rhizosphere (R.i), were added to potting soils with salt concentrations of 0 (CK) and 100 mmol·L⁻¹ for a tomato plant cultivation experimentation. Physiological and photosynthetic properties of the plants were monitored. Result The AMF-treatments increased superoxide dismutase (SOD) activity by 47.4%, peroxidase (POD) activity by 32.9%, catalase (CAT) activity by 35.7%, malondialdehyde (MDA) content by 61.8%, and proline (PRO) content by 6% of the tomato plants under the imposed salt stress. Meanwhile, the mycorrhizal infection rate and photosynthetic intensity decreased by 27.8% and 54.6%, respectively. The inoculation effectively enhanced the resistance of the host plants to high salinity. The proline content of the tomato plants grown on the F.m-inoculated soil declined 60.7%, which was 2.2 times of that on R.i-inoculated counterpart. The net photosynthetic rate (P_n) and stomatal conductance (G_s) of the tomato plants on the F.m-inoculated soil increased by 49.1% and 35.4%, respectively, which were 1.4 times of R.i-inoculation. In addition, the damage to the key photosynthesis enzymes was reduced, as the maximum increase rate of RuBP carboxylase in the tomato plants rose to 31.2% under the F.m-treatment and 1.1 times of which under R.i. Conclusion The introduction of AMF in soil could delay the adverse effect of high salinity on the physiological activities, improve the photosynthetic carbon assimilation and salt tolerance, and promote the growth of tomato plants. Of the two candidates, F.m was shown to significantly superior to R.i for the application.
- Arbuscular mycorrhizal fungi /
- tomato /
- infection rate /
- physiological activity /
- salt stress

HTML全文

图 1 不同AMF对盐胁迫下番茄菌根侵染率的影响

Figure 1. Effects of AMF on mycorrhizal infection rate of tomato plants under salt stress

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图 2 盐胁迫下AMF处理后对番茄叶绿素荧光参数和光合气体参数影响

Figure 2. Effects of AMF-treatment on chlorophyll fluorescence and photosynthetic gas parameters of tomato plants under salt stress

下载: 全尺寸图片幻灯片

图 3 盐胁迫下AMF处理后对番茄FBPase活性与RuBP酶活性的影响

Figure 3. Effects of AMF-treatment on FBPase and RuBP activities of tomato plants under salt stress

下载: 全尺寸图片幻灯片

表 1 不同AMF对盐胁迫下番茄菌根依赖性及侵染密度的影响

Table 1. Effects of AMF on mycorrhizal dependence and infection density of tomato plants under salt stress

项目　　 Items	处理组 Treatments	F.m	R.i	C.e	D.v
菌根依赖性 Mycorrhizal dependence/%	0 mmol·L⁻¹	54.79±0.09 a	50.87±0.01 ab	29.07±0.01 c	49.05±0.02 b
菌根依赖性 Mycorrhizal dependence/%	100 mmol·L⁻¹	129.06±0.11 a	128.09±0.24 a	111.37±0.15 b	127.08±0.33 ab
侵染密度 Infection density/%	0 mmol·L⁻¹	4.01±0.24 a	4.02±0.11 ab	0.76±0.09 c	3.01±0.07 b
侵染密度 Infection density/%	100 mmol·L⁻¹	0.79±0.01 a	0.82±0.03 b	0.13±0.02 c	0.80±0.04 b
注：同项同行数据后不同字母表示差异显著（ P ＜0.05），下同。 Note: Values followed by different lowercase letters within a column indicate significant difference at 5% level, the same as below.

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表 2 盐胁迫下AMF处理后番茄抗氧化酶系统的变化

Table 2. Changes on antioxidant enzymes of tomato plants under AMF-treatment against salt stress

测定指标 Measured indicators	处理组 Treatments	时间 Times/d
测定指标 Measured indicators	处理组 Treatments	15	30	45
SOD/ （U·g⁻¹·min⁻¹ ）	CK 0	4.76±0.24 e	5.09±0.15 d	4.74±0.19 e
	R.i 0	5.34±0.08 e	5.49±0.12 cd	5.55±0.08 d
	F.m 0	6.17±0.26 d	6.30±0.26 c	6.17±0.23 c
	CK 100	7.29±0.28 c	9.73±0.43 b	7.05±0.10 b
	R.i 100	8.37±0.16 b	10.58±0.41 b	7.41±0.40 b
	F.m 100	9.99±0.34 a	12.21±0.30 a	8.46±0.17 a
POD/ （U·g⁻¹·min⁻¹ ）	CK 0	73.61±4.37 d	74.91±3.64 d	85.30±3.67 f
	R.i 0	83.00±2.33 dc	137.14±3.34 c	132.22±4.69 e
	F.m 0	84.31±1.63 c	146.53±4.69 c	148.79±3.18 d
	CK 100	273.11±2.73 b	422.00±3.93 b	565.50±4.13 c
	R.i 100	347.73±2.71 a	424.44±2.48 b	614.80±3.84 b
	F.m 100	355.44±3.83 a	468.63±4.00 a	649.68±5.54 a
CAT/ （U·g⁻¹·min⁻¹ ）	CK 0	33.13±1.11 d	35.17±1.06 d	37.13±1.32 e
	R.i 0	37.86±2.15 cd	37.79±1.69 d	43.54±1.33 d
	F.m 0	40.32±1.93 c	44.72±1.51 c	49.28±1.23 d
	CK 100	48.74±1.76 b	74.68±1.79 b	84.25±2.28 c
	R.i 100	54.25±1.34 b	78.19±1.47 b	93.07±2.80 b
	F.m 100	61.14±2.63 a	88.19±1.98 a	99.25±1.68 a

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表 3 盐胁迫下AMF处理后脯氨酸及MDA含量的变化

Table 3. Proline and MDA contents after AMF-treatment under salt stress

测定指标 Measured indicators	处理组 Treatments	时间 Times/d
测定指标 Measured indicators	处理组 Treatments	15	30	45
丙二醛含量 MDA content/ （mmol·g⁻¹）	CK 0	3.94±0.14 d	6.81±0.13 d	7.25±0.18 d
	CK 100	7.28±0.23 a	10.44±0.15 a	17.68±0.38 a
	F.m 0	3.91±0.15 e	6.76±0.10 d	6.88±0.20 d
	F.m 100	4.56±0.15 c	8.96±0.19 c	12.37±0.34 c
	R.i 0	3.93±0.10 de	6.79±0.16 d	7.16±0.16 d
	R.i 100	6.02±0.17b	9.59±0.21 b	14.12±0.29 b
脯氨酸含量 Pro content/ （μg·g⁻¹）	CK 0	42.41±1.58 d	6.81±0.13 cd	7.25±0.18 bc
	CK 100	35.82±2.06 d	10.44±0.15 d	17.68±0.38 d
	F.m 0	41.00±3.55 d	6.76±01.0 d	6.88±0.20 c
	F.m 100	228.57±4.33 a	8.96±0.19 a	12.37±0.34 a
	R.i 0	118.06±4.18 c	6.79±0.16 c	7.16±0.16 d
	R.i 100	181.43±3.32 b	9.59±0.21 b	14.12±0.29 b

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表 4 不同处理组番茄盐害指数的影响变化

Table 4. Changes on salt injury index of tomato plants under treatments

处理组 Treatments	盐害指数 Salt injury index
CK	70.26±2.23 a
F.m	35.63±5.15 c
R.i	51.23±3.33 b

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