8个番石榴品种的转录组测序

张丽梅; 陈钟佃; 张朝坤; 魏秀清; 许家辉

doi:10.19303/j.issn.1008-0384.2021.11.008

8个番石榴品种的转录组测序

doi: 10.19303/j.issn.1008-0384.2021.11.008

1.
福建省农业科学院果树研究所, 福建　福州　350013
2.
福建省农业科学院农业生态研究所, 福建　福州　350013
3.
福建省漳州市农业科学研究所, 福建　漳州　363000

基金项目: 福建省农业科学院科技创新团队项目（CXTD2021008-2）；中国热带农业科学院基本科研业务费专项项目（1630032020013）；福建省科技计划区域发展项目（2016N3021)

详细信息

作者简介:
张丽梅（1974−），女，副研究员，研究方向：果树栽培、生理生化与分子技术（E-mail: lm155195@sina.com）

通讯作者:
许家辉（1971−），男，硕士，研究员，研究方向：果树育种、栽培及生理生化（E-mail: xjhui577@163.com）

中图分类号: S 667
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出版历程
- 收稿日期: 2021-06-27
- 修回日期: 2021-12-08
- 网络出版日期: 2021-12-30
- 刊出日期: 2021-11-28

Transcriptome Sequencing on Eight Psidium guajava Cultivars

1.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
3.
Zhangzhou Institute of Agricultural Sciences of Fujian Province, Zhangzhou, Fujian　363000, China

摘要

摘要: 目的获得番石榴转录本数据，探讨不同番石榴品种转录本之间差异。方法研究通过Illumina HiSeq 2500平台对红心（HX）、彩虹（CH）、紫红（ZH）、水晶（SJ）、秀珍（XZ）、彩叶变（CYB）、彩叶（CY）和紫星（ZX）等8个番石榴品种的8～9成熟果肉进行转录组测序，通过组装及数据库比对，获得大量番石榴转录本注释信息。结果组装获得了126 979条 Unigene，平均长度766 bp，N₅₀为1654 bp，N₉₀为267 bp；8个番石榴品种间共有27 274个基因为共表达基因。通过主成分分析与差异基因表达聚类分析，发现品种水晶（SJ）和彩叶变（CYB）之间基因功能表达差异较小，品种紫红（ZH）与其他品种之间基因表达差异较大。聚类分析将品种水晶（SJ）和彩叶变（CYB）聚为一类，品种红心（HX）、彩虹（CH）、秀珍（XZ）、彩叶（CY）、紫星（ZX）归为一类，但品种紫红（ZH）与其他品种间距离较远。结论本研究结果为后续番石榴种质资源分类、功能基因表达、功能基因鉴定提供了大量可靠的转录组数据。
- 番石榴 /
- 转录组 /
- 测序 /
- 功能注释
Abstract: Objective Transcriptomes of different guava cultivars were obtained for comparison. Methods Transcriptome sequencing by Illumina Hwaseq 2 500 was conducted on 8-9 mature fruit pulp from 8 varieties of Psidium guajava Linn, namely Red Heart (HX), Rainbow (CH), Purplish Red (ZH), Crystal (SJ), Shuzhen (XZ), Colored-leaf B (CYB), Colored-leaf (CY), and Purple Star (ZX). Results By comparing to the database, 126979 Unigenes of an average length of 766 bp with a N₅₀ of 1654 bp and a N₉₀ of 267 bp were identified. There were 27274 genes co-expressed in the 8 cultivars. The PCA and differential gene expression cluster analyses showed that the differences on the function expressions between the genes of SJ and CYB was less than those between ZH and other cultivars. SJ and CYB were clustered in a category and HX, CH, XZ, CY, and ZX in a different category, while ZH significantly differentiated from the other cultivars. Conclusion The substantial amount of transcription data secured from this study could be useful for the classification as well as the expression and identification of functional genes of guava germplasms.
- Psidium guajava Linn. /
- transcriptome /
- sequencing /
- functional annotation

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图 1 Transcript（A）和Unigene（B）长度分布

Figure 1. Distributions of transcript length (A) and Unigene length (B)

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图 2 番石榴Unigene NR数据库注释分析

Figure 2. Annotation of NR database on guava Unigenes

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图 3 番石榴Unigene Go分类

Figure 3. GO functional classification of guava Unigenes

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图 4 番石榴Unigene的KOG数据库分类

Figure 4. KOG classification of guava Unigenes

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图 5 番石榴Unigene的KEGG数据库分类

Figure 5. KEGG classification of guava Unigenes

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图 6 番石榴PCA主成分分析

Figure 6. PCA on guava

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图 7 样本遗传距离分析

Figure 7. Heat map of distance analysis on guava samples

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图 8 番石榴基因共表达韦恩图

Figure 8. TPM Venn diagram of guava cultivars

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表 1 样本质量控制数据统计

Table 1. Statistics on quality control data of samples

品种 Cultivars	总碱基数 Number of bases/bp	Reads/bp	Q₃₀/%	GC/%
红心HX	7123935342	145	95.96	49.98
彩虹CH	8029343436	144	95.47	50.68
紫红ZH	6300972075	145	95.57	50.10
水晶SJ	7681478290	145	95.60	51.23
秀珍XZ	5184950713	144	95.31	50.27
彩叶变CYB	7824848106	146	95.82	50.65
彩叶CY	7607494989	144	95.35	50.44
紫星ZX	6364083214	144	95.50	50.49

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表 2 番石榴8个品种间基因表达差异统计分析

Table 2. Statistical analysis on gene expressions of 8 guava cultivars

对照组 CK	试验组 Experimental cultivars	上调基因数 Up-regulatory genes	下调基因数 Down-regulatory genes	KEGG富集途径 KEGG pathway
对照组 CK	试验组 Experimental cultivars	上调基因数 Up-regulatory genes	下调基因数 Down-regulatory genes	1	2	3	4
SJ	ZX	2601	8289	Y	Y	Y	N
SJ	ZH	5959	11471	Y	Y	Y	N
SJ	CH	1996	7354	Y	Y	Y	N
SJ	XZ	1994	7178	Y	Y	Y	N
CYB	CY	2640	6327	Y	Y	Y	N
SJ	HX	2187	8443	N	Y	Y	Y
ZX	ZH	4876	4232	Y	Y	N	Y
CH	HX	13355	1896	Y	N	N	N
注：KEGG富集途径1：核糖体；2：碳代谢；3：氨基酸生物合成；4：淀粉和蔗糖代谢。Y表示富集到该代谢途径，N表示未富集到该代谢途径。 Note: KEGG pathway 1: ribosome; 2: carbon metabolism; 3: amino acid biosynthesis; 4: starch and sucrose metabolisms; Y: enriched; N: non-enriched.

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