Effects of Calcium Fertilizers and Application Methods on Quality of Kyoho Grapes
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摘要:
目的 研究不同钙肥及其施用方式对巨峰葡萄果实品质的影响,为生产栽培提供理论参考。 方法 以4年生避雨设施栽培巨峰葡萄为试材,以叶面、果穗喷施及叶面、果穗喷施结合根际灌施2种方式,分别施用1500倍糖醇钙和800倍硝酸钙2种不同的钙肥,测定巨峰葡萄成熟过程中的果皮着色、不同部位钙含量、可溶性固形物质、总糖、总酸含量及果粒硬度等指标。 结果 钙肥施用对提升巨峰葡萄外观着色、内在品质及不同部位钙含量等均起到一定作用。不同处理间以叶面、果穗喷施结合根际灌施糖醇钙肥处理果皮着色较深;糖酸比值44.63;总酚、花色苷、共价结合型果胶及离子结合型果胶含量显著高于对照;果皮与果肉钙含量分别为对照的1.36倍和1.73倍;果肉硬度比对照高出19.28%;可溶性固形物较对照提升5.71%。 结论 钙处理对提升巨峰葡萄内外在果实品质具有一定作用,以叶面、果穗喷施结合根际灌施1500倍糖醇钙肥处理对巨峰葡萄鲜果品质提升效果最佳,可在生产中推广应用。 Abstract:Objective Effects of different calcium fertilizers and application methods on the fruit quality of Kyoho grape were studied to provide guidelines for the production and cultivation. Method Four-year-old Kyoho grape vines were cultivated in a rain shelter facility to observe the results under treatments of varied calcium fertilizers and applications. Two different calcium fertilizers, 1 500 x sugar alcohol calcium and 800 x calcium nitrate, were used to either spray on the leaves and/or fruit ears or combine the spraying with rhizosphere irrigation. Grape peel color, calcium contents in different parts, and soluble solid, total sugar, and total acid contents, as well as firmness of the fruits were monitored. Result The fertilizing method affected both the peel color change and pulp quality as well as the calcium content in different parts of the grapes. When the leaves and fruit ears were sprayed and accompanied by rhizosphere irrigation with the sugar alcohol calcium fertilizer, the pericarp color was intensified. The contents of total phenols, anthocyanins, covalently bound pectin, and ion-bound pectin of the grapes also increased to become significantly higher than those of control. The calcium contents in peel and pulp were 1.36 and 1.73 times of control, respectively; the flesh firmness 19.28% higher than that of control; and the soluble solid mass fraction 5.71% higher than that of control. Conclusion The calcium fertilizations improved both internal and external quality of Kyoho grapes. By combining the foliar-and-ear spraying with rhizosphere irrigation using the 1,500x sugar alcohol calcium fertilizer, significant improvements on the fresh fruit quality could be realized. -
Key words:
- calcium /
- leaf fertilizer /
- irrigation /
- Kyoho grape /
- quality
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表 1 施钙对巨峰葡萄果实品质的影响
Table 1. Effect of calcium application on Kyoho grape quality
处理
Treatment总糖
Total sugar
content/%总酸
Total acid
content/%糖酸比
Ratio of total sugar
to total acid总酚
Total phenol/
(mg·g−1)花色苷
Anthocyanins/
(µg·g−1)共价结合型果胶
Covalently bonded pectin/
(mg·g−1)离子结合型果胶
Ion-bound pectin/
(mg·g−1)A+C 13.46 bB 0.36 aA 37.39 bB 4.77b B 303.65 aA 181.70 bB 152.90 bB A+C+D 14.28 aA 0.32 bB 44.63 aA 4.89 aA 313.53 aA 186.05 aA 170.03 aA B+C 13.28 bB 0.37 aA 35.89 bB 4.79 bB 303.84 aA 159.67 cC 148.86 bB B+C+D 14.06 aA 0.32 bB 43.94 aA 4.90 aA 296.74 bA 183.40 aAB 162.09 aA CK 13.27 bB 0.37 aA 35.86 bB 4.87 aAB 256.35 cB 150.00 dC 148.40 bB 注:(1)A+C:叶面、果穗喷施糖醇钙肥;A+C+D:叶面、果穗喷施结合根际灌施糖醇钙肥; B+C:叶面、果穗喷施硝酸钙肥;B+C+D:叶面、果穗喷施结合根际灌施硝酸钙肥;CK:清水喷施。(2)数据为巨峰葡萄最后一次采样,即花后95 d的测定数据。(3)同列数据后不同大小写字母分别表示不同处理间差异达极显著水平(P<0.01)和显著水平(P< 0.05)。(4)表中数据为2020年数据。表2~7同。
Note: (1) A+C: Spraying sugar alcohol calcium fertilizer on ear; A+C+D: Foliar-and-ear spraying combined with rhizosphere irrigation using sugar alcohol calcium fertilizer; B+C: Spraying calcium nitrate fertilizer on leaves and ears; B+C+D: Foliar-and-ear spraying combined with rhizosphere irrigation using calcium nitrate fertilizer; CK: Spraying with clean water. (2) Data collected at last sampling, i.e., 95d after flowering. (3) Data with different uppercase and lowercase letters on same column indicate extremely significant differences (P < 0.01) and significant level (P <0.05), respectively. (4) Data presented were collected in 2020. Same for Tables 2-7.表 2 施钙对巨峰葡萄果皮着色的影响
Table 2. Effect of calcium application on Kyoho grape peel color
处理
TreatmentL a b C H a/b A+C 29.57 aA 7.74 cC −1.13 dD 7.14 cC 350.71 aAB 6.85 bB A+C+D 27.99 bB 8.76 aA −1.94 aA 8.03 bB 347.77 aAB 4.52 eE B+C 30.23 aA 7.04 dD −1.29 cC 8.75 aA 347.28 aAB 5.46 cC B+C+D 27.05 cB 8.27 bB −1.70 bB 7.28 cC 357.93 aA 4.86 dD CK 30.16 aA 7.04 dD −0.49 eE 7.15 cC 353.23 aA 14.37 aA 表 6 施钙对巨峰葡萄果实硬度的影响
Table 6. Effect of calcium application on firmness of Kyoho grapes
(单位:kg·cm −2) 处理
Treatment花后70 d
70 d after flowering花后75 d
75 d after flowering花后80 d
80 d after flowering花后85 d
85 d after flowering花后90 d
90 d after flowering花后95 d
95 d after floweringA+C 0.472 bA 0.452 bB 0.400 dC 0.394 cD 0.368 cC 0.338 dD A+C+D 0.462 bA 0.434 cC 0.423 bcB 0.419 bBC 0.414 aA 0.396 aA B+C 0.471 aA 0.464 aA 0.458 aA 0.439 aA 0.395 bB 0.383 bB B+C+D 0.472 abA 0.444 bB 0.425 bB 0.422 aAB 0.387 bB 0.365 cC CK 0.443 cB 0.419 dC 0.408 cBC 0.407 bCD 0.361 cC 0.332 eD 表 3 施钙对巨峰葡萄果皮钙含量的影响
Table 3. Effects of calcium application on calcium content in Kyoho grape peel
(单位:µg·g−1) 处理
Treatment花后70 d
70 d after flowering花后75 d
75 d after flowering花后80 d
80 d after flowering花后85 d
85 d after flowering花后90 d
90 d after flowering花后95 d
95 d after floweringA+C 224 cC 234 cC 260 cC 293 dD 302 dD 298 cC A+C+D 289 aA 306 aAB 356 aA 388 aA 403 aA 370 aA B+C 253 bB 280 bB 282 bB 321 cC 334 cC 324 bB B+C+D 241 bB 303 aA 347 aA 364 bB 370 bB 366 aA CK 215 cC 227 cC 248 dC 258 eE 268 eE 270 dD 表 4 施钙对巨峰葡萄果肉钙含量的影响
Table 4. Effect of calcium application on calcium content in Kyoho grape pulp
(单位:µg·g−1) 处理
Treatment花后70 d
70 d after flowering花后75 d
75 d after flowering花后80 d
80 d after flowering花后85 d
85 d after flowering花后90 d
90 d after flowering花后95 d
95 d after floweringA+C 5.2 cC 9.0 bB 10.0 cC 11.4 cC 12.3 dD 12.2 dC A+C+D 5.0 cC 9.7 aA 15.6 aA 18.9 aA 22.7 aA 21.6 aA B+C 6.2 aA 8.3 cC 14.0 bB 16.2 bB 18.2 cC 14.0 cB B+C+D 5.9 bB 9.0 bB 13.7 bB 16.4 bB 19.0 bB 14.6 bB CK 4.5 dD 5.8 dD 10.3 cC 11.7 cC 12.1 eD 12.5 dC 表 5 施钙对巨峰葡萄果粒重量的影响
Table 5. Effect of calcium application on weight of individual Kyoho grape
(单位:g) 处理
Treatment花后70 d
70 d after flowering花后75 d
75 d after flowering花后80 d
80 d after flowering花后85 d
85 d after flowering花后90 d
90 d after flowering花后95 d
95 d after floweringA+C 9.93 bAB 10.27 aA 10.52 cBC 10.81 bcB 10.93 aA 11.11 aA A+C+D 10.04 aA 10.36 aA 10.61 bB 10.88 bAB 11.03 aA 11.08 aA B+C 9.74 bcB 9.93 bA 10.30 cC 10.64 dC 10.85 aA 11.02 aA B+C+D 9.66 cB 10.02 abA 10.31 cC 10.58 cdBC 10.93 aA 11.07 aA CK 10.15 aA 10.52 aA 10.87 aA 10.99 aA 11.09 aA 11.19 aA 表 7 施钙对巨峰葡萄果实可溶性固形物含量的影响
Table 7. Effect of calcium application on soluble solids content of Kyoho grapes
(单位:%) 处理
Treatment花后70 d
70 d after flowering花后75 d
75 d after flowering花后80 d
80 d after flowering花后85 d
85 d after flowering花后90 d
90 d after flowering花后95 d
95 d after floweringA+C 16.23 bcAB 16.70 abA 17.37 abA 17.54 aAB 17.81 abA 17.70 cB A+C+D 16.35 aA 16.77 aA 17.63 aA 17.87 aA 18.50 aA 18.27 aA B+C 15.99 bcAB 16.33 bA 17.17 bA 17.50 aA 17.93 bA 17.87 bcAB B+C+D 16.12 abAB 16.57 bA 17.61 abA 17.98 aA 18.33 abA 18.07 abAB CK 15.89 cB 16.07 abA 16.76 bA 17.35 bB 17.50 bA 17.77 cB -
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