杂交稻广优673高产栽培因子优化与抗瘟性分析

李福德

doi:10.19303/j.issn.1008-0384.2021.10.002

杂交稻广优673高产栽培因子优化与抗瘟性分析

doi: 10.19303/j.issn.1008-0384.2021.10.002

李福德

福建省安溪县农业农村局，福建　安溪　362400

基金项目: 福建省农作物新品种示范推广项目（2019）

详细信息

作者简介:
李福德（1965−），男，高级农艺师，主要从事作物新品种及栽培技术研究（E-mail：fdsir@yeah.net）

中图分类号: S 511；S 436
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- 被引次数: 0
出版历程
- 收稿日期: 2021-08-09
- 修回日期: 2021-10-01
- 网络出版日期: 2021-12-30
- 刊出日期: 2021-10-28

High-yield Cultivation Factors Optimizing and Blast-resistance Analysis for Hybrid Rice Variety Guangyou 673

LI Fude

Anxi Agricultural and Rural Bureau, Anxi, Fujian　362400, China

摘要

摘要: 目的分析杂交稻品种广优673的栽培试验技术和稻瘟病抗性基因的来源，为水稻抗病新品种选育和广优673推广种植提供依据。方法以广优673为试验材料，运用最优设计进行密度、施氮量、秧龄3项栽培因子试验，建立产量与3项措施的回归模型，分析栽培措施对产量的影响，并利用稻瘟病抗性基因Pi2、Pi9和Pigm已开发的功能标记，开展稻瘟病抗性基因的分析和检测。结果广优673水稻秧龄短的，早插本田生长期长，需施用较多氮肥，有利于高产；而秧龄长的，本田生长期相应短些，不需施用过多的氮肥。密度、施氮量、秧龄3项栽培因子对广优673的产量都有影响，综合高产栽培措施为：每公顷插20.52万～27.48万丛，每公顷施纯氮144.70～183.76 kg，秧龄26～33 d。稻瘟病抗性基因检测与分析表明广优673含有稻瘟病抗性基因Pi2。结论杂交稻广优673具有高产、抗病、生育期适中等特点，产量≥8250 kg·hm⁻²的栽培因子模拟寻优结果为：每公顷插24.00万丛，每公顷施纯N 164.23 kg，秧龄29.5 d，并推测稻瘟病抗性主要来源于稻瘟病抗性基因Pi2。
- 杂交稻 /
- 广优673 /
- 特征特性 /
- 栽培试验 /
- 稻瘟病抗性
Abstract: Objective In this study, essential agronomical practices of the hybrid rice variety Guangyou 673 and its resistance to rice blast were analyzed to provide a scientific basis for breeding new rice varieties with improved disease-resistant and to further expand the cultivation of Guangyou 673 variety. Methods Guangyou 673 was introduced for cultivation in Anxi County of Fujian Province. The impact of three essential agronomical practices, i.e., planting density (x₁), nitrogen fertilization rate (x₂) and seedling age (x₃), on the performance of Guangyou 673 was evaluated. Regression models between grain yield and the above three agronomical factors were estimated to analyze the impact of agronomical practices on grain yield. The developed functional markers of the rice blast-resistance genes Pi2, Pi9 and Pigm were used to identify the blast-resistance genes in the genetic background of Guangyou 673. Results All the three studied agronomical practices revealed significant effects on grain yield of Guangyou 673. The results showed that shortening the seedling age which was with a long growth period necessitates more nitrogen fertilization to achieve higher grain yield. On the contrary, lengthening the seedling age which was with shorter growth period required less nitrogen fertilization. The maximum grain yield was achieved when planting density ranged from 2.052–2.748×10⁶clusters per ha, N fertilizer 144.70–183.76 kg·hm⁻², and seedling age ranged from 26–33 days. Genotyping of Guangyou 673 with the functional molecular markers of Pi2, Pi9 and Pigm genes revealed that the variety Guangyou 673 contained the Pi2 blast-resistance gene. Conclusion Hybrid rice variety Guangyou 673 had great characteristics of high grain yield, blast resistance and moderate growth period. The high yield more than 8 250 kg·hm⁻² could be achieved with N fertilizer 144.70–183.76 kg ·hm⁻², average plant density 2.4×10⁶clusters per ha, and growth period 26–33 days. The results further suggested that Pi2 gene maybe the main source of blast resistance in Guangyou 673.
- hybrid rice /
- Guangyou 673 /
- characteristics /
- culture test /
- blast resistance

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图 1 抗稻瘟病基因的分子标记检测

注：M，D2000 marker；P1，广优673；P2，日本晴；P3， 9311；P4，CO39。

Figure 1. PCR analysis for rice blast resistance genes

Note: M, D2000 marker; P1, Guangyou 673; P2, Nipponbare; P3, 9311; P4, CO39.

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表 1 因素水平及编码

Table 1. Code and levels of factors

编码值 Code value			实际值 Actual value
x₁密度 Density	x₂ N肥 N fertilizer	x₃ 秧龄 Seedling age	x₁密度 Density/ (万丛·hm⁻²)	x₂N肥 N fertilizer/ (kg·hm⁻²)	x₃秧龄 Seedling age/d
−2	−2	−2	7.50	0.00	20
−1.414	−1.414	−1	11.85	67.50	27
0	0	0	22.50	135.00	34
1.414	1.414	1	33.15	202.50	41
2	2	2	37.50	270.00	48

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表 2 栽培试验的产量结果

Table 2. Yield of cultivation experiment

编号 Number	x₁ 密度 Density	x₂ 施N量 Nitrogen	x₃秧龄 Seedling age	产量 Yield/（kg·hm⁻²）
编号 Number	x₁ 密度 Density	x₂ 施N量 Nitrogen	x₃秧龄 Seedling age	小区1 Plot 1	小区2 Plot2
1	0	0	2	6388.20	6597.69
2	0	0	−2	8576.30	8786.80
3	−1.414	−1.414	1	7233.70	7567.90
4	1.414	−1.414	1	7672.40	7890.67
5	−1.414	1.414	1	7180.20	7398.67
6	1.414	1.414	1	8490.56	8100.85
7	2	0	−1	8065.40	8180.04
8	−2	0	−1	8260.45	8342.10
9	0	2	−1	8089.41	8168.67
10	0	−2	−1	6299.73	6082.61
11	0	0	0	9170.11	9380.67

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表 3 偏回归系数的t值检验

Table 3. The t test of partial regression coefficient

偏回归系数 Partial regression coefficent	bx₁	bx₂	bx₃	bx₁x₂	bx₁x₃	bx₂x₃	bx₁²	bx₂²	bx₃²
t	2.11	5.85	5.73	1.64	3.05	4.35	3.43	9.08	7.24
显著水平 Significant level	0.10	0.01	0.01	0.20	0.05	0.01	0.01	0.01	0.01

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表 4 每公顷产量 ≥8250 kg 措施分布范围

Table 4. Distribution range of factors when yield ≥8250 kg·hm⁻²

因素 Factor	x₁密度 Density/（万丛·hm⁻²）	x₂施N量 Nitrogen rate/（kg·hm⁻²）	x₃秧龄 Seedling age/d
编码区域 Coding region	−0.264～0.664	0.144～0.722	−0.704～−0.096
农艺措施范围 Range of agronomic measures	20.52～27.48	144.70～183.76	26～33
平均 Average	24.00	164.23	29.5

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参考文献(17)

[1]	ROYCHOWDHURY M, JIA Y L, CARTWRIGHT R. Structure, function, and co-evolution of rice blast resistance genes [J]. Acta Agronomica Sinica, 2012, 38(3): 381−393. doi: 10.3724/SP.J.1006.2012.00381
[2]	张佩胜, 赵春德, 余宁, 等. 稻瘟病抗性基因的克隆及应用研究进展 [J]. 中国稻米, 2014, 20(5):1−7. doi: 10.3969/j.issn.1006-8082.2014.05.001 ZHANG P S, ZHAO C D, YU N, et al. Recent progress on cloning and application of rice blast resistance genes [J]. China Rice, 2014, 20(5): 1−7.(in Chinese) doi: 10.3969/j.issn.1006-8082.2014.05.001
[3]	ZHANG N, LUO J, ROSSMAN A Y, et al. Generic names in Magnaporthales [J]. IMA Fungus, 2016, 7(1): 155−159. doi: 10.5598/imafungus.2016.07.01.09
[4]	胡朝芹, 刘剑宇, 王韵茜, 等. 粳稻子预44抗LP11稻瘟病菌基因pizy6(t)的定位 [J]. 植物学报, 2017, 52(1):61−69. doi: 10.11983/CBB16126 HU C Q, LIU J Y, WANG Y Q, et al. Mapping of Pizy6(t), a gene conferring resistance to the rice blast strain LP11, in Oryza sativa subsp.japonica cultivar Ziyu44 [J]. Chinese Bulletin of Botany, 2017, 52(1): 61−69.(in Chinese) doi: 10.11983/CBB16126
[5]	张晓慧, 冯晓敏, 林少扬. 水稻主栽品种空育131抗稻瘟病位点的扫描及其基因组重构建 [J]. 植物学报, 2017, 52(1):30−42. doi: 10.11983/CBB16107 ZHANG X H, FENG X M, LIN S Y. Scanning for Pi loci and rebuilding an improved genome of elite rice variety kongyu 131 [J]. Chinese Bulletin of Botany, 2017, 52(1): 30−42.(in Chinese) doi: 10.11983/CBB16107
[6]	杨德卫, 李生平, 崔海涛, 等. 寄主植物与病原菌免疫反应的分子遗传基础 [J]. 遗传, 2020, 42(3):278−294. YANG D W, LI S P, CUI H T, et al. Molecular genetic mechanisms of interaction between host plants and pathogens [J]. Hereditas, 2020, 42(3): 278−294.(in Chinese)
[7]	杨德卫, 王莫, 韩利波, 等. 水稻稻瘟病抗性基因的克隆、育种利用及稻瘟菌无毒基因研究进展 [J]. 植物学报, 2019, 54(2):265−276. doi: 10.11983/CBB18194 YANG D W, WANG M, HAN L B, et al. Progress of cloning and breeding application of blast resistance genes in rice and avirulence genes in blast fungi [J]. Chinese Bulletin of Botany, 2019, 54(2): 265−276.(in Chinese) doi: 10.11983/CBB18194
[8]	茆诗松, 丁元, 周纪芗, 等. 回归分析及其试验设计[M]. 2版. 上海: 华东师范大学出版社, 1981: 23-68.
[9]	田大刚, 王锋, 陈松彪, 等. 一种稻瘟病抗性基因座Pi2/9功能基因分子标记及其应用: 中国, CN201810054310. X[P] . 2021-03-26.
[10]	朱永生, 董瑞霞, 谢鸿光, 等. 高产抗病杂交稻新品种广优673的选育 [J]. 福建农业学报, 2018, 33(7):683−686. ZHU Y S, DONG R X, XIE H G, et al. Breeding high-yield, high-resistance hybrid rice, guangyou 673 [J]. Fujian Journal of Agricultural Sciences, 2018, 33(7): 683−686.(in Chinese)
[11]	徐中儒. 回归分析与试验设计[M]. 北京: 中国农业出版社, 1998: 58-154.
[12]	佟立伟. 多元统计分析计算机程序[M]. 北京: 中国农业科学技术出版社, 1995: 3-88.
[13]	CHEN H L, CHEN B T, ZHANG D P, et al. Pathotypes of Pyricularia grisea in rice fields of central and Southern China [J]. Plant Disease, 2001, 85(8): 843−850. doi: 10.1094/PDIS.2001.85.8.843
[14]	LIU G, LU G, ZENG L, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6 [J]. Molecular Genetics and Genomics, 2002, 267(4): 472−480. doi: 10.1007/s00438-002-0677-2
[15]	ZHOU B, QU S H, LIU G F, et al. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea [J]. Molecular Plant Microbe Interactions, 2006, 19(11): 1216−1228. doi: 10.1094/MPMI-19-1216
[16]	DENG Y W, ZHAI K R, XIE Z, et al. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance [J]. Science, 2017, 355(6328): 962−965. doi: 10.1126/science.aai8898
[17]	QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice [J]. Genetics, 2006, 172(3): 1901−1914. doi: 10.1534/genetics.105.044891