Biocontrol Effect of Brevibacillus brevis FJAT-0809-GLX against Lasiodiplodia theobromae on Longan
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摘要:
目的 探明短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌生长的抑制作用。 方法 采用抑菌圈法和抑菌率法测定短短芽胞杆菌对龙眼焦腐病菌的拮抗活性，观察其对龙眼焦腐病菌菌丝生长和形态的影响。采用龙眼果实回接法，测定短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌的生防效果，并分析其对龙眼果皮防御酶活性的影响。 结果 短短芽胞杆菌FJAT-0809-GLX发酵上清液对龙眼焦腐病菌菌丝生长具有抑制作用，抑菌圈直径为15.77 mm。随着菌株培养时间的延长和添加量的增多，其对龙眼焦腐病菌生长的抑制效果也逐渐增强，添加50 mL时，抑菌率可达74.17%。接种龙眼焦腐病菌3 d后，好果率为68.33%，且龙眼果皮SOD酶和CAT酶活性均明显高于对照。 结论 短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌生长具有较强的抑制作用，在开发龙眼病害生物防治菌株方面具有一定的潜力。 Abstract:Objective Inhibitory effect of a strain of Brevibacillus brevis against Lasiodiplodia theobromae (Pat.) Griffon & Maubl on longan fruits was evaluated. Method In vitro antagonistic activity of B. brevis FJAT-0809-GLX was observed by the inhibition zone and rate on the mycelial growth and morphology of L. theobromae. Efficacy of the strain for biocontrol on the pathogen was determined by in vivo experiments, as well as the activities of defense enzymes in longan peel. Result Supernatant of the fermentation broth showed a pathogenic inhibition zone diameter of 15.77 mm and an inhibition rate that increased with increased fermentation time or treatment dosage. The inhibition rates reached 74.17% when 50 mL of the supernatant was applied. The supernatant inoculation on longan fruits significantly reduced the disease spreading rendering a healthy fruit rate of 68.33%. Meanwhile, the activities of superoxide dismutase and hydrogen peroxidase in the treated fruits became significantly higher than those of control. Conclusion B. brevis FJAT-0809-GLX significantly inhibited the growth of L. theobromae as shown by in vitro plating as well as in the field challenge test. It demonstrated a potential venue for bio-controlling the disease on longan. -
图 1 发酵上清液和菌体对龙眼焦腐病菌的抑菌圈
注：1：菌体；2：发酵上清液。
Figure 1. L. theobromae inhibition zone shown on medium by fermentation supernatant or bacteria cells
Note: 1: B. brevis FJAT-0809-GLX cells; 2: fermentation supernatant.
图 2 不同培养时间发酵上清液对龙眼焦腐病菌的抑菌圈
Figure 2. L. theobromae inhibition zones on medium treated with supernatants of varied fermentation time
图 3 不同添加量上清液对龙眼焦腐病菌生长的抑制效果
Figure 3. Inhibition of fermentation supernatant in varied dosages on growth of L. theobromae
图 4 短短芽胞杆菌FJAT-0809-GLX对龙眼焦腐病菌菌丝形态的影响
Figure 4. L. theobromae mycelial growth on medium with B. brevis FJAT-0809-GLX
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[1] 张居念, 林河通, 谢联辉, 等. 龙眼焦腐病菌及其生物学特性 [J]. 福建农林大学学报(自然科学版), 2005, 34(4):425−429.ZHANG J N, LIN H T, XIE L H, et al. Biological characteristics of Lasiodiplodia theobromae Pat. causing black-rotten disease of longan fruit [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2005, 34(4): 425−429.(in Chinese) [2] 唐建阳, 车建美, 刘波, 等. 龙眼采后腐烂相关细菌与真菌的分离与鉴定 [J]. 保鲜与加工, 2013, 13(5):9−14. doi: 10.3969/j.issn.1009-6221.2013.05.002TANG J Y, CHE J M, LIU B, et al. Isolation and identification of bacterial and fungal strains from the rotting Dimocarpus longan Lour. [J]. Storage and Process, 2013, 13(5): 9−14.(in Chinese) doi: 10.3969/j.issn.1009-6221.2013.05.002 [3] WANG J, HADDAD N I A, YANG S Z, et al. Structural characterization of lipopeptides from Brevibacillus brevis HOB1 [J]. Applied Biochemistry and Biotechnology, 2010, 160(3): 812−821. doi: 10.1007/s12010-009-8536-9 [4] YE J, HUA Y, HUI P, et al. Biosorption and biodegradation of triphenyltin by Brevibacillus brevis [J]. Bioresource Technology, 2012, 129C(2): 236−241. [5] PANDA A K, BISHT S S, DEMONDAL S, et al. Brevibacillus as a biological tool: A short review [J]. Antonie Van Leeuwenhoek, 2014, 105(4): 623−639. doi: 10.1007/s10482-013-0099-7 [6] NEHRA V, SAHARAN B S, CHOUDHARY M. Evaluation of Brevibacillus brevis as a potential plant growth promoting rhizobacteria for cotton (gossypium hirsutum) crop[J]. Springerplus, 5(1): 948-958. [7] 付保荣, 刘梦琦, 张润洁, 等. 短短芽孢杆菌(Brevibacillus brevis)对鲤鱼养殖水质及其生长特性的影响 [J]. 生态科学, 2018, 37(5):149−154.FU B R, LIU M Q, ZHANG R J, et al. Effects of Brevibacillus brevis on aquiculture water quality and carps growth characteristics [J]. Ecological Science, 2018, 37(5): 149−154.(in Chinese) [8] 周海燕, 饶力群, 吴永尧. 甘露聚糖酶man23基因的重组及其在短短芽孢杆菌中的表达 [J]. 浙江大学学报: 农业与生命科学版, 2008(4):39−44.ZHOU H Y, RAO L Q, WU Y Y. Recomination of mannanase gene man23 and its expression in Brevibacillus brevis [J]. Journal of Zhejiang University, 2008(4): 39−44.(in Chinese) [9] 龚国利, 王忠忠. 短短芽孢杆菌的鉴定及其抑菌物质的初步研究 [J]. 陕西科技大学学报, 2017, 35(4):126−131. doi: 10.3969/j.issn.1000-5811.2017.04.024GONG G L, WANG Z Z. Identification of Brevibacillus brevis strain and preliminary study on its antibacterial substance. [J]. Journal of Shanxi University of Science and Technology, 2017, 35(4): 126−131.(in Chinese) doi: 10.3969/j.issn.1000-5811.2017.04.024 [10] 刘莎莎, 程园园, 张丹,等. 两株紫花苜蓿根际芽孢杆菌的筛选及生防效果研究 [J]. 草业学报, 2015, 24(9):96−103. doi: 10.11686/cyxb2015026LIU S S, CHENG Y Y, ZHANG D, et al. Isolation, identification, and biocontrol effects of Bacillus spp. from the rhizosphere of alfalfa [J]. Acta Prataculturae Sinica, 2015, 24(9): 96−103.(in Chinese) doi: 10.11686/cyxb2015026 [11] CHE J M, LIU B, LIN Y Z, et al. Draft genome sequence of biocontrol bacterium Brevibacillus brevis strain FJAT-0809-GLX [J]. Genome Announcements, 2013, 1(2): e0016013. [12] CHE J M, LIU B, CHEN Z, et al. Identification of ethylparaben as the antimicrobial substance produced by Brevibacillus brevis FJAT-0809-GLX [J]. Microbiological Research, 2015, 172: 48−56. doi: 10.1016/j.micres.2014.11.007 [13] 车建美, 付萍, 刘波, 等. 保鲜功能微生物FJAT-0809-GLX对龙眼保鲜特性的研究 [J]. 热带作物学报, 2010, 31(9):1632−1640. doi: 10.3969/j.issn.1000-2561.2010.09.034CHE J M, FU P, LIU B, et al. The preservation feature of a freshness-keeping function bacterium strain FJAT-0809-GLX to Dimocarpus longanan Lour [J]. Chinese Journal of Tropical Crops, 2010, 31(9): 1632−1640.(in Chinese) doi: 10.3969/j.issn.1000-2561.2010.09.034 [14] 车建美, 郑雪芳, 刘波, 等. 短短芽胞杆菌FJAT-0809-GLX菌剂的制备及其对枇杷保鲜效果的研究 [J]. 保鲜与加工, 2011, 11(5):6−9. doi: 10.3969/j.issn.1009-6221.2011.05.003CHE J M, ZHENG X F, LIU B, et al. Preparation of Brevibacillus brevis FJAT-0809-GLX agent and study on its effect on Loquats (Eriobotrya japonica) [J]. Storage and Process, 2011, 11(5): 6−9.(in Chinese) doi: 10.3969/j.issn.1009-6221.2011.05.003 [15] 王薇, 李泳, 王伟, 等. Y-S-Y12发酵液和生物质热解液混配对辣椒炭疽病的防治及作用机理 [J]. 中国农业科技导报, 2020, 22(10):129−138.WANG W, LI Y, WANG W, et al. Anti-disease effect and mechanism of Y-S-Y12 strain fermentation mixed with biomass pyrolysis solution on pepper anthracnose [J]. Journal of Agricultural Science and Technology, 2020, 22(10): 129−138.(in Chinese) [16] 付岗, 叶云峰, 吴永官, 等. 7株拮抗菌的鉴定及其对香蕉炭疽病的防治作用 [J]. 热带作物学报, 2015, 36(2):358−364. doi: 10.3969/j.issn.1000-2561.2015.02.023FU G, YE Y F, WU Y G, et al. Identification and control effect on banana anthracnose of seven antagonistic strains [J]. Chinese Journal of Tropical Crops, 2015, 36(2): 358−364.(in Chinese) doi: 10.3969/j.issn.1000-2561.2015.02.023 [17] WAFAA M H, EL KAREEM F K A, SHABAAN A A H. Bioprocessing of Brevibacillus brevis and Bacillus polymyxa: a potential biocontrol agents of gray mould disease of strawberry fruits [J]. Current Opinion in Biotechnology, 2013, 24(1): S45. [18] CHE J M, LIU B, RUAN C Q, et al. Biocontrol of Lasiodiplodia theobromae, which causes black spot disease of harvested wax apple fruit, using a strain of Brevibacillus brevis FJAT-0809-GLX [J]. Crop Protection, 2015, 67: 178−183. doi: 10.1016/j.cropro.2014.10.012 [19] 伍善东, 雷平, 郭照辉, 等. 1株番茄青枯病内生拮抗细菌的分离鉴定及盆栽防效 [J]. 湖南农业大学学报(自然科学版), 2016, 42(6):627−630.WU S D, LEI P, GUO Z H, et al. Isolation, identification and potted control efficacy of an endophytic bacerial angainst Ralstionia solanacearum [J]. Journal of Hunan Agricultural University(National Sciences), 2016, 42(6): 627−630.(in Chinese) [20] 张平, 李朝阳, 赵清泉, 等. 生防细菌对油茶炭疽病病原菌的抑制作用 [J]. 北京林业大学学报, 2020, 42(10):107−116.ZHANG P, LI C Y, ZHAO Q Q, et al. Inhibition effects of biocontrol bacteria strains on the pathogen of Camellia oleifera anthracnose [J]. Journal of Beijing Forestry University, 2020, 42(10): 107−116.(in Chinese) [21] 张琼, ZABIHULLAH S, 唐灿明. 贝莱斯芽孢杆菌SZAD1对大丽轮枝菌的生物防治效果 [J]. 棉花学报, 2020, 32(4):329−338. doi: 10.11963/1002-7807.zqtcm.20200609ZHANG Q, ZABIHULLAH S, TANG C M. Biocontrol effect of Bacillus velezensis strain SZAD1 on Verticillium dahliae [J]. Cotton Science, 2020, 32(4): 329−338.(in Chinese) doi: 10.11963/1002-7807.zqtcm.20200609 [22] GAJBHIYE A, RAI A R, MESHRAM S U, et al. Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum [J]. World Journal of Microbiology and Biotechnology, 2010, 26(7): 1187−1194. doi: 10.1007/s11274-009-0287-9 [23] WANG F, XIAO J, ZHANG Y, et al. Biocontrol ability and action mechanism of Bacillus halotolerans against Botrytis cinerea causing grey mould in postharvest strawberry fruit [J]. Postharvest Biology and Technology, 2021, 174: 111456. doi: 10.1016/j.postharvbio.2020.111456 [24] WANG X. Biocontrol efficacy of Bacillus siamensis LZ88 against brown spot disease of tobacco caused by Alternaria alternata [J]. Biological Control, 2020: 154. [25] LI C, HU W, PAN B, et al. Rhizobacterium Bacillus amyloliquefaciens SQRT3 induced systemic resistance controls bacterial wilt in tomato [J]. Pedosphere, 2017, 27: 1135−1146. doi: 10.1016/S1002-0160(17)60406-5 [26] JINAL N H, AMARESAN N. Evaluation of biocontrol Bacillus species on plant growth promotion and systemic-induced resistant potential against bacterial and fungal wilt-causing pathogens [J]. Archives of Microbiology, 2020, 202(7): 1785−1794. doi: 10.1007/s00203-020-01891-2 [27] 李君, 冯小飞, 赵宁, 等. 拮抗芽孢杆菌对核桃诱导抗性酶活性的影响 [J]. 中国植保导刊, 2020, 40(9):5−11. doi: 10.3969/j.issn.1672-6820.2020.09.001LI J, FENG X F, ZHAO N, et al. Effect of antagonistic Bacillus on the antivity of induced resis tance enzyme in walnut [J]. China Plant Protection, 2020, 40(9): 5−11.(in Chinese) doi: 10.3969/j.issn.1672-6820.2020.09.001 -
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