番茄细菌性髓部坏死病病原细菌的鉴定

陈梅春; 肖荣凤; 陈燕萍; 郑雪芳; 朱育菁; 王阶平; 刘波

doi:10.19303/j.issn.1008-0384.2021.09.011

番茄细菌性髓部坏死病病原细菌的鉴定

doi: 10.19303/j.issn.1008-0384.2021.09.011

福建省农业科学院农业生物资源研究所，福建　福州　350003

基金项目: 福建省自然科学基金项目（2019J01117）；福建省科技计划公益类专项（2019R1034-5、2020R1034007）

详细信息

作者简介:
陈梅春（1985−），女，博士，助理研究员，研究方向：生物活性物质研究（E-mail：cmczjw@163.com）

通讯作者:
朱育菁（1972−），女，博士，研究员，研究方向：植物保护（E-mail：zyjingfz@163.com）

刘波（1957-），男，博士，研究员，研究方向：生物防治（E-mail：fzliubo@163.com）

中图分类号: S 436
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出版历程
- 收稿日期: 2021-02-24
- 修回日期: 2021-06-25
- 网络出版日期: 2021-08-10
- 刊出日期: 2021-09-28

Identification of a Pathogen of Bacterial Pith Necrosis in Tomatoes

Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

摘要

摘要: 目的对2017–2019年在福建、浙江等地大棚种植的番茄上发现的一种引起番茄细菌性髓部坏死病的新病害进行病原鉴定，为该病害的防治奠定基础。方法采用Biolog法、16S rDNA基因序列测定及MALDI-TOF-MS等方法对分离的病原菌进行种属鉴定；利用柯赫氏法则进行病原菌的致病性测定。结果从田间番茄细菌性髓部坏死病发病植株上分离保存了2个代表性细菌菌株，将2个菌株接种至番茄幼苗上，它们均可侵染番茄植株，造成茎杆维管束褐变，茎髓部组织腐烂、空心，并最终导致整株植株萎蔫死亡。该症状与田间自然发病症状相同，并从回接发病植株上重新分离到相同的病原细菌。经Biolog法、16S rDNA基因序列测定及MALDI-TOF-MS等方法确认该病原菌为阿氏肠杆菌（Enterobacter asburiae）。结论福建、浙江等地大棚种植的番茄上发现的引起番茄细菌性髓部坏死病的病原菌为阿氏肠杆菌，该发现尚属国内外首次报道。
- 番茄髓部坏死病 /
- 病原鉴定 /
- Biolog法 /
- MALDI-TOF-MS分析 /
- 阿氏肠杆菌
Abstract: Objective The pathogen that caused the bacterial pith necrosis on greenhouse tomatoes grown in Fujian and Zhejiang provinces during 2017-2019 was identified. Method Biolog analysis, 16S rDNA gene sequencing, and MALDI-TOF-MS were performed to identify the pathogen. The Koch’s rule was employed to determine the pathogenicity of the isolate. Result Two bacteria strains were isolated from the diseased tomatoes. The symptoms of discolored, dark brown vascular tissue and pith of main stem observed on tomatoes in the laboratory were identical to those found in the field. The bacteria were re-isolated from the infected plants and identified as Enterobacter asburiae. Conclusion The pathogen that caused bacterial pith necrosis in tomatoes in Fujian and Zhejiang was identified and reported as E. asburiae for the first time.
- Tomato pith necrosis /
- pathogen identification /
- Biolog analysis /
- MALDI-TOF-MS /
- Enterobacter asburiae

HTML全文

图 1 田间番茄细菌病害症状

注：a: 整株; b: 茎部；c: 茎部剖面。

Figure 1. Symptoms of tomato bacterial pith necrosis diseases in the field

Note: a: plant; b: stem; c: section of stem.

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图 2 病菌形态

注：a: LB培养基上菌株形态；b: 菌株透射电镜图。

Figure 2. Morphological characteristics of bacterial isolates

Note: a: bacterial isolates on LB; b: bacterial isolates on transmission electron microscopic scan.

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图 3 室内病菌回接番茄植株的髓坏死病症状

注：a：7 天；b：20 天。

Figure 3. Symptoms of bacterial pith necrosis on inoculated tomato plants in greenhouse

Note: a: 7 d; b: 20 d.

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图 4 菌株FJAT-46819 和FJAT-47134的16 S rDNA基因进化树分析

Figure 4. Phylogenetic tree based on partial 16S rDNA gene sequences of FJAT-46819 and FJAT-47134

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表 1 Biolog分析结果

Table 1. Results of biology analysis

编号 No.	参数 Parameters			物种 Species
编号 No.	可能性 PROB	相似性 SIM	位距 DIS	物种 Species
FJAT-46819	0.5	0.5	6.0	肠杆菌属（Enterobacter spp.）
FJAT-47134	0.6	0.6	5.1	肠杆菌属（Enterobacter spp.）

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表 2 菌株FJAT-46819 和FJAT-47134的MALDI-TOF-MS鉴定结果

Table 2. Results of MALDI-TOF-MS analysis on FJAT-46819 and FJAT-47134

编号 No.	鉴定 Identification	菌株名称 Strains	匹配值（可信度） Matching value （credibility score）	标记 Mark
FJAT-46819	Enterobacter asburiae CCM 4032 CCM	阿氏肠杆菌	2.326	+++
FJAT-47134	Enterobacter asburiae CCM 4032 CCM	阿氏肠杆菌	2.333	+++
注：匹配值2.300-3.000表示菌株鉴定结果可靠（标记为“+++”）。 Note: Matching values of 2.300-3.000, marked by +++, indicate accurate identification of tested strain.

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