高通量测序分析根结线虫对马铃薯根际土壤真菌群落多样性的影响

张丽芳; 李艳琼; 蔡建; 高秀; 徐晴芳; 刘威良; 杨卫星; 胡海林

doi:10.19303/j.issn.1008-0384.2021.08.010

高通量测序分析根结线虫对马铃薯根际土壤真菌群落多样性的影响

doi: 10.19303/j.issn.1008-0384.2021.08.010

1.
曲靖师范学院云南省高校特色果酒技术创新与应用工程研究中心，云南　曲靖　655011
2.
昆明学院农学与生命科学学院，云南　昆明　650214
3.
曲靖师范学院数学与统计学院，云南　曲靖　655011

基金项目: 云南省科技计划计划青年项目（2018FD080）；曲靖师范学院青年项目（2019QN002）

详细信息

作者简介:
张丽芳（1987−），女，博士，讲师，研究方向：植物病理（E-mail：752859175@qq.com）

通讯作者:
胡海林（1986−），男，硕士，助教，研究方向：马铃薯病害（E-mail：243902676@qq.com）

中图分类号: S 154; S 532
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- 被引次数: 0
出版历程
- 收稿日期: 2021-03-08
- 修回日期: 2021-06-19
- 网络出版日期: 2021-08-10
- 刊出日期: 2021-08-28

High-Throughput Sequencing on Fungal Diversity in Potato Rhizosphere Soil Infested by Root-knot Nematodes

1.
Yunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing, Yunnan　655011, China
2.
College of Agronomy and Life Sciences, Kunming University, Kunming, Yunnan　650214, China
3.
College of Mathematics and Statistics, Qujing Normal University, Qujing, Yunnan　655011, China

摘要

摘要: 目的分析根结线虫对马铃薯根际土壤真菌群落多样性的影响。方法通过高通量测序技术、土壤性质测定及生物信息学技术分析感染根结线虫马铃薯和健康马铃薯根际土壤真菌群落多样性差异。结果从6个土壤样品中共获得3界12门34纲77目171科332属526种的土壤真菌。对物种多样性指数分析后，发现感染根结线虫马铃薯病株与健康马铃薯植株的根际土壤真菌群落多样性和土壤性质均存在显著性差异。对6个样品进行物种组成分析后，在门水平中，感病植株根际土壤真菌Basidiomycota丰度较健康植株根际土壤真菌丰度高，Ascomycota丰度低于健康植株根际土壤；在科水平中，病株根际土壤丰度较高的菌群有Nectriaceae和Trimorphomycetaceae；在属水平中，健康植株根际土壤丰度较高的菌群为镰刀菌属Fusarium、被孢霉属Mortierella和未知分类毛壳菌科属unclassified_f_Chaetomiaceae。结论感染根结线虫的马铃薯与健康马铃薯根际土壤真菌群落丰度在门、科和属水平上均存在差异，为进一步研究马铃薯根结线虫的发生和防治提供了新的思路。
- 根结线虫 /
- 马铃薯根际土壤 /
- 土壤性质 /
- 高通量测序 /
- 真菌多样性
Abstract: Objective Diversity of the rhizosphere soil fungal community in potato field as affected by the presence of root-knot nematodes was investigated using high-throughput sequencing technique. Methods The properties and bioinformatics of rhizosphere soils surrounding healthy and infested potato plants were compared to analyze the possible effects of differential fungal community on the occurrence and control of the disease. Results There were 526 fungi species belonging to 332 genera, 171 families, 77 orders, 34 classes, 12 phyla, and 3 kingdoms identified from the 6 field specimens. Significantly differentiations on the fungal community diversity and properties of the rhizosphere soils on the healthy and infested potato lots were found. In the infected areas, Basidiomycota was the more abundant phylum, but Ascomycota less, than on the healthy lots. At family level, Nectriaceae and Trimorphomycetaceae were more abundantly found in the soil of diseased plants, while at genus level, Fusarium, Mortierella, and some unclassified Chaetomiaceae more richly in the soil of healthy plants. Conclusion Significant differences in the abundance of fungi in the rhizosphere soils of potatoes infested by the root-knot nematodes and the healthy counterparts were found at phylum, family, and genus levels. The information would aid further study on the occurrence and control of potato root-knot nematodes.
- Root-knot nematodes /
- potato rhizosphere soils /
- soil properties /
- high throughout sequencing technology /
- fungi diversity

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图 1 97%相似度时土壤样品的稀释曲线

Figure 1. Dilution plots of soil specimens at 97% similarity

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图 2 两组样品中土壤真菌韦恩图

Figure 2. Venn diagram on fungi identified in two sample groups

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图 3 门水平上真菌群落结构

Figure 3. Structure of fungi community at phylum level

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图 4 科水平上真菌群落结构

Figure 4. Structure of fungi community at family level

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图 5 属水平上真菌群落结构

Figure 5. Structure of fungi community at genus level

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表 1 不同样品土壤性质

Table 1. Properties of soil specimens

样品编号 Sample ID	pH	有机质 OM/（g·kg⁻¹）	速效磷 AP/（mg·kg⁻¹）	速效钾 AK/（mg·kg⁻¹）	速效氮 AN/（mg·kg⁻¹）
MLE1	4.72±0.05 b	1.33±0.20 b	277.37±0.78 c	423.51±0.32 bc	57.39±0.28 de
MLE2	4.62±0.03 b	1.27±0.14 b	265.43±0.63 d	420.19±0.19 c	56.12±0.25 e
MLE3	4.80±0.02 b	1.28±0.30 b	278.54±0.24 c	425.85±0.18 ab	60.44±0.19 cd
MLCK1	5.13±0.13 a	2.33±0.33 a	286.83±0.56 b	426.92±0.45 ab	66.41±0.78 a
MLCK2	5.30±0.32 a	2.82±0.18 a	293.35±0.36 a	427.32±0.67 ab	64.00±0.57 ab
MLCK3	5.22±0.46 a	2.74±0.36 a	279.72±0.67 c	428.28±0.46 a	61.23±0.42 bc
注：同列数据后不同小写字母表示差异显著（P＜0.05），表2同。 Note: Different uppercase letters in the same column represented significant difference（P＜0.05）, The same as Table 2.

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表 2 不同样品土壤真菌群落的多样性指数

Table 2. Diversity indices on fungal communities in soil specimens

样品编号 Sample ID	原始序列 Original sequences	有效序列 Effective sequences	有效序列率 Effective data ratio	多样性指数 Diversity index		丰富度指数 Richness index		覆盖率 Coverage
样品编号 Sample ID	原始序列 Original sequences	有效序列 Effective sequences	有效序列率 Effective data ratio	Shannon-Wiener指数 Shannon-Wiener index	Simpson指数 Simpson index	Ace指数 Ace index	Chao指数 Chao index	覆盖率 Coverage
MLE1	52315	51714	0.989	2.83±0.10 c	0.18±0.04 b	744.88±0.42 d	677.05±0.78 d	0.997
MLE2	56393	55653	0.987	2.59±0.14 c	0.26±0.03 a	574.26±0.25 e	586.08±0.36 e	0.997
MLE3	65004	64006	0.985	3.64±0.32 b	0.06±0.01 c	731.93±0.12 d	763.33±0.25 c	0.997
MLCK1	64811	63193	0.975	4.13±0.05 a	0.06±0.01 c	960.73±0.36 c	975.25±0.65 b	0.997
MLCK2	68180	65857	0.966	4.09±0.23 a	0.05±0.01 c	977.19±0.83 b	978.84±0.45 b	0.997
MLCK3	68295	66066	0.967	4.04±0.14 a	0.06±0.02 c	1013.80±0.46 a	1024.59±0.88 a	0.997
注：数据后不同小写字母表示处理间差异达显著水平 Note:Data with different lowercase letters indicated significant difference (P＜0.05).

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