生长素IAA诱导红萍结孢的研究

徐国忠; 郑向丽; 王俊宏; 邓素芳; 杨有泉; 应朝阳

doi:10.19303/j.issn.1008-0384.2021.06.014

生长素IAA诱导红萍结孢的研究

doi: 10.19303/j.issn.1008-0384.2021.06.014

徐国忠^{1, 2,},
郑向丽^{1, 2},
王俊宏^{1, 2},
邓素芳^{1, 2},
杨有泉^{1, 2},
应朝阳^{1, 2}

1.
福建省农业科学院农业生态研究所，福建　福州　350013
2.
国家红萍种质资源中心，福建　福州　350013

基金项目: 福建省科技计划公益类专项（2018R1010-2）；现代农业产业技术体系建设专项（CARS-22）

详细信息

作者简介:
徐国忠（1965−），男，研究员，主要从事生态农业研究（E-mail：xuguozhong178@126.com）

中图分类号: S 555.1
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- 被引次数: 0
出版历程
- 收稿日期: 2021-01-18
- 修回日期: 2021-05-24
- 网络出版日期: 2021-06-06
- 刊出日期: 2021-06-28

Auxin IAA-Induced Azolla Sporulation

XU Guozhong^{1, 2
,},
ZHENG Xiangli^{1, 2},
WANG Junhong^{1, 2},
DENG Sufang^{1, 2},
YANG Youquan^{1, 2},
YING Zhaoyang^{1, 2}

1.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
National Azolla Germplasm Resource Center, Fuzhou, Fujian　350013, China

摘要

摘要: 目的探索生长素IAA诱导红萍结孢的可行性。方法在网室自然条件下进行，采用质量浓度为0（对照）、1、5、10、15、20 μg·mL⁻¹的生长素（IAA）对生长期的红萍1001、3001和4018进行不同浓度喷施处理，每周喷施一次，至结孢出现停止喷施。止喷施。结果 IAA处理5个月后，1、5和10 μg·mL⁻¹的细绿萍1001开始结孢，质量浓度为 5 μg·mL⁻¹时，结孢率、孢子果数量、孢子果䧳雄比均优于其他处理及对照。在5 μg·mL⁻¹时，结孢率为15.0%、孢子果数量为6.31个、孢子果䧳雄比为0.62∶1；在10 μg·mL⁻¹时，结孢率为6.3%、孢子果数量为4.12个、孢子果䧳雄比为0.53∶1；在1 μg·mL⁻¹时，结孢率为4.7%、孢子果数量为3.90个、孢子果䧳雄比为0.51∶1。其他品种、处理都未能诱导出孢子果。细绿萍1001经IAA处理后，在 1、5和10 μg·mL⁻¹时萍体的大小和C/N比有明显提高，其余处理和对照无显著差异；卡州萍3001和小叶萍4018处理萍体大小和C/N比均无显著差异。结论生长素IAA能够诱导细绿萍1001结孢，其中IAA质量浓度为5 μg·mL⁻¹时诱导效果最佳，可在生产上进行应用。
- 激素 /
- 生长素 /
- 红萍 /
- 结孢 /
- 诱导
Abstract: Objective Auxin IAA-induced sporulation of Azolla was investigated. Method Under natural conditions in a netted chamber, auxin IAA solutions in concentration of 0 (control), 1, 5, 10, 15, or 20 μg·mL⁻¹ was sprayed once-a-week on Azolla filiculoides Lamarck 1001, A. caroliniana wild 3001, and A. microphylla Kaulfus 4018 during vegetative growth period for the duration until sporocarps appeared . Result After five months of spraying with 1, 5, or 10 μg·mL⁻¹ IAA solution, sporocarps began to form only on Azolla 1001. The sporulation rate, spore count, and female/male ratio were 15.0%, 6.31, and 0.62 ∶ 1, respectively, under the 5 μg·mL⁻¹ application, which significantly differed from 6.3%, 4.12, and 0.53 ∶ 1, respectively, under the 10 μg·mL⁻¹ application, 4.7%, 3.90, and 0.51 ∶ 1, respectively, under the 1 μg·mL⁻¹ application, and control. In addition, the size and C/N ratio of Azolla 1001 significantly increased by the spraying, as compared to control, whereas none observed on the other two ferns. Conclusion Auxin IAA could induce the sporulation of A. filiculoides Lamarck 1001. Spraying IAA at the concentration of 5 μg·mL⁻¹ on the ferns appeared feasible for field application.
- hormone /
- auxin /
- Azolla /
- sporulation /
- induction

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表 1 生长素IAA对3种不同红萍品种（系）结孢的诱导结果

Table 1. IAA-induced sporulation on 3 Azolla cultivars (lines)

激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	是否结孢 The sporulation of Azolla
激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	1001	3001	4018
IAA	0（CK）	否	否	否
IAA	1	是	否	否
IAA	5	是	否	否
IAA	10	是	否	否
IAA	15	否	否	否
IAA	20	否	否	否

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表 2 生长素IAA对3种红萍萍体大小（长度和宽度）的影响

Table 2. Effects of IAA applications on size (length and width) of 3 Azolla cultivars (lines)

激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	长度 Length/mm			宽度 Breadth/mm
激素 Hormone	质量浓度 Treatment concentration/ (μg·mL⁻¹)	1001	3001	4018	1001	3001	4018
IAA	0（CK）	24.32±0.24 Cc	14.07±0.09 Aa	24.26±0.10 Aa	11.95±0.03 Cc	7.99±0.04 Aa	12.01±0.03 Aa
IAA	1	33.90±0.09 Bb	14.08±0.04 Aa	24.24±0.10 Aa	15.98±0.02 Bb	8.00±0.03 Aa	12.00±0.04 Aa
IAA	5	34.97±0.05 Aa	14.13±0.09 Aa	24.25±0.12 Aa	16.30±0.09 Aa	8.01±0.05 Aa	12.01±0.02 Aa
IAA	10	34.05±0.21 Bb	14.12±0.04 Aa	24.32±0.12 Aa	16.02±0.03 Bb	7.97±0.02 Aa	12.04±0.03 Aa
IAA	15	24.46±0.12 Cc	14.16±0.04 Aa	24.28±0.07 Aa	12.02±0.03 Cc	7.99±0.05 Aa	12.00±0.05 Aa
IAA	20	24.55±0.09 Cc	14.14±0.04 Aa	24.40±0.06 Aa	12.02±0.03 Cc	8.00±0.03 Aa	12.03±0.03 Aa
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P＜0.05), capital letters indicate very significant differences between treatments (P＜0.01).

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表 3 生长素IAA对3种红萍C/N比例的影响

Table 3. Effects of IAA applications on C/N ratio of 3 Azolla cultivars

序号 No.	IAA质量浓度 IAA concentration/ (μg·mL ⁻¹)	C/N比例 The ratio of C and N
序号 No.	IAA质量浓度 IAA concentration/ (μg·mL ⁻¹)	1001	3001	4018
1	0（CK）	10.3±0.2 Cc	9.6±0.7 Aa	10.7±0.7 Aa
2	1	15.5±0.5 Bb	9.7±0.7 Aa	10.6±0.5 Aa
3	5	18.0±1.0 Aa	9.5±0.7 Aa	10.8±0.4 Aa
4	10	16.2±1.1 ABb	9.8±0.7 Aa	10.9±0.5 Aa
5	15	10.5±0.6 Cc	9.6±0.6 Aa	10.8±0.5 Aa
6	20	10.6±0.5 Cc	9.6±0.3 Aa	10.9±0.7 Aa
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P ＜0.05), those followed by capital letters, extremely significant differences between treatments (P＜0.01).

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表 4 生长素IAA对细绿萍1001的诱导效果

Table 4. Effect of IAA on sporulation induction of Azolla 1001

激素 Hormone	处理质量浓度 Treatment concentration/ (μg·mL⁻¹)	结孢率 Sporocarp formation rate/%	结孢数量 The sporocarps number/个	䧳雄比 The sporocarp female male ratio
IAA	0（CK）	0.00±0.00Cd	0.00±0.00 Dd	0.00±0.00 Cc
IAA	1	4.70±0.60Bc	3.90±0.08 Cc	0.51±0.02 Bb
IAA	5	15.00±2.00 Aa	6.31±0.17 Aa	0.62±0.02 Aa
IAA	10	6.30±0.60 Bb	4.12±0.02 Bb	0.53±0.03 Bb
IAA	15	0.00±0.00 Cd	0.00±0.00 Dd	0.00±0.00 Cc
IAA	20	0.00±0.00 Cd	0.00±0.00 Dd	0.00±0.00 Cc
注：同列数值后不同小写字母表示处理间差异显著（P＜0.05），大写字母表示处理间差异极显著（P＜0.01）。 Note: Values followed by different lowercase letters indicate significant difference among treatments (P ＜0.05), those followed by capital letters, extremely significant differences between treatments (P＜0.01).

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参考文献(30)

[1]	刘中柱, 郑伟文. 中国满江红[M]. 北京: 农业出版社, 1989: 1 − 28.
[2]	陈坚, 徐国忠. 满江红属系统学研究的新进展 [J]. 植物学通报, 2001, 36(4):485−489. CHEN J, XU G Z. The progress in systematic studies of Azolla [J]. Chinese Bulletin of Botany, 2001, 36(4): 485−489.(in Chinese)
[3]	陆培基, 徐国忠, 林永辉. 红萍资源的征集与保存 [J]. 作物品种资源, 1994(2):19−20. LU P J, XU G Z, LIN Y H. Collection and preservation of Azolla [J]. Crop variety resources, 1994(2): 19−20.(in Chinese)
[4]	宋未, 平淑珍, 尤崇杓. 应用¹⁵N示踪技术研究红萍对水稻的肥效 [J]. 核农学通报, 1991, 12(5):212−215. SONG W, PING S Z, YOU C B. The fertilizer effect of Azolla on rice was studied by using ¹⁵N tracer technique [J]. Nuclear agriculture bulletin, 1991, 12(5): 212−215.(in Chinese)
[5]	翁伯琦, 陈炳焕, 唐建阳, 等. ¹⁵N示踪法研究稻萍鱼体系中红萍氮素的利用及对水稻生长的影响 [J]. 福建省农科院学报, 1987, 2(1):16−24. WENG B Q, CHEN B H, TANG J Y, et al. Study of utilization and effection of Azolla nitrogen with ¹⁵N isotope labelled method in the rice-Azolla-fish cropping system [J]. Fujian Journal of Agricultural Sciences, 1987, 2(1): 16−24.(in Chinese)
[6]	张伟光, 翁伯琦, 唐建阳, 等. 用¹⁵N示踪法研究红萍在稻田中的肥效 [J]. 福建农业科技, 1986(5):8−10. ZHANG W G, WENG B Q, TANG J Y, et al. Study on the fertilizer effect of Azolla in rice field with ¹⁵N isotope labeled method [J]. Fujian Agricrltural Science and Technology, 1986(5): 8−10.(in Chinese)
[7]	翁伯琦, 陈炳焕, 唐建阳, 等. 施用尿素、红萍对水稻生长及其氮素利用效率的影响 [J]. 核农学报, 1988, 2(2):93−101. WENG B Q, CHEN B H, TANG J Y, et al. Effect of applying urea and green manure Azolla on the rice growing and nitrogen utllization effeciency [J]. Acta Agriculturae Nucleatae Sinica, 1988, 2(2): 93−101.(in Chinese)
[8]	翁伯琦. 亚洲若干国家的红萍研究和应用 [J]. 世界农业, 1986(8):48. WENG B Q. Research and application of Azolla in some Asian countries [J]. World Agriculture, 1986(8): 48.(in Chinese)
[9]	刘彩玲, 刘珠, 何春梅, 等. 福建稻区紫云英播期及播种量对其产量和养分含量的影响 [J]. 福建农业学报, 2020, 35(4):443−449. LIU C L, LIU Z, HE C M, et al. Effects of sowing time and seeding rate on yield and quality of Chinese milk vetch in Fujian [J]. Fujian Journal of Agricultural Sciences, 2020, 35(4): 443−449.(in Chinese)
[10]	李娟, 张立成, 张华, 等. 福建花生氮磷钾推荐施肥与有机肥替代化肥潜力研究 [J]. 福建农业学报, 2020, 35(5):480−488. LI J, ZHANG L C, ZHANG H, et al. Recommended NPK and organic fertilizer applications for peanut farming in Fujian [J]. Fujian Journal of Agricultural Sciences, 2020, 35(5): 480−488.(in Chinese)
[11]	文志强, 林阿典, 廖卿, 等. 秸秆还田与客土改良酸性植烟土壤及提升烟叶产质量的效果 [J]. 福建农业学报, 2020, 35(6):649−656. WEN Z Q, LIN A D, LIAO Q, et al. Improvement effects of straw returning and mixed purple soils on acidic soil and yield & quality of flue-cured tobacco [J]. Fujian Journal of Agricultural Sciences, 2020, 35(6): 649−656.(in Chinese)
[12]	NAGALINGUM N S, SCHNEIDER H, PRYER K M. Comparative morphology of reproductive structures in heterosporous water ferns and a reevaluation of the sporocarp [J]. International Journal of Plant Sciences, 2006, 167(4): 805−815. doi: 10.1086/503848
[13]	TUHINA DEY VAISHAMPAYAN A. Growth and sporulation/spore germination studies in genetically modified Azolla pinnata[J]. Environment and Ecology. MKK Publication, Calcutta, India: 2005.23: Spl-1, 4-7.
[14]	BINU KOSHY SHIVAPRAKASH M K, VIDYA KULKARNI. Effect of antibacterial and antifungal agents on mega sporocarp germination in Azolla filiculoides[J]. Journal of Ecobiology. Palani Paramount Publications, Palani, India: 2001.13: 2, 119-122.
[15]	JANES R. Growth and survival of Azolla filiculoides in Britain. II. sexual reproduction [J]. New Phytologist, 1998, 138(2): 377−384. doi: 10.1046/j.1469-8137.1998.00113.x
[16]	SINGH P K, SINGH D P, SINGH R P. Germination of water fern Azolla caroliniana sporocarps at varied light, amino acids, sugars and abscisic acid[J]. Proceedings of the Indian National Science Academy. Part B, Biological Sciences. 1996.62: 5, 353-358.
[17]	唐龙飞, 郑德英. 我国红萍育种概况及其展望 [J]. 福建省农科院学报, 1993, 8(1):40−44. TANG L F, ZHENG D Y. The general situation and prospect for Azolla breeding in China [J]. Fujian Journal of Agricultural Sciences, 1993, 8(1): 40−44.(in Chinese)
[18]	湖南农科院土肥所, 常德县科协, 常德县石板滩公社古堤坪大队. 蕨状满江红孢子果丰产及提纯技术的研究 [J]. 湖南农业科学, 1982(3):30−33. Institute of soil fertilizer, Hunan academy of agricultural sciences. Study on the high yield and purification technology of Azolla filiculoides Lamarck sporocarp [J]. Hunan Agricultural Sciences, 1982(3): 30−33.(in Chinese)
[19]	湖南农科院土壤肥料研究所. 蕨状满江红周年产孢规律的研究 [J]. 湖南农业科学, 1981(3):19−20. Institute of soil fertilizer, Hunan academy of agricultural sciences. Study on the regularity of annual sporulation of Azolla filiculoides Lamarck [J]. Hunan Agricultural Sciences, 1981(3): 19−20.(in Chinese)
[20]	徐国忠, 郑向丽. 满江红孢子果几种贮藏方法比较 [J]. 福建农业科技, 2002(1):18. doi: 10.3969/j.issn.0253-2301.2002.01.012 XU G Z, ZHENG X L. Comparison of several storage methods of Azolla sporocarp [J]. Fujian Agricrltural Science and Technology, 2002(1): 18.(in Chinese) doi: 10.3969/j.issn.0253-2301.2002.01.012
[21]	金桂英, 陈坚, 唐龙飞. 满江红三膘亚属种及种间杂种雄性育性初步研究 [J]. 福建农业学报, 2003, 18(1):56−58. doi: 10.3969/j.issn.1008-0384.2003.01.013 JIN G Y, CHEN J, TANG L F. Male fertility of species and interspecific hybrid among subgenus Euazolla [J]. Fujian Journal of Agricultural Sciences, 2003, 18(1): 56−58.(in Chinese) doi: 10.3969/j.issn.1008-0384.2003.01.013
[22]	鲁雪华, 卞祖良, 郭文杰, 等. 满江红孢子果空间诱变效应的研究 Ⅱ空间条件对不同品系满江红耐荫性的影响 [J]. 江西农业大学学报(自然科学), 2002, 24(6):833−837. LU X H, BIAN Z L, GUO W J, et al. Research on the effects of space induction of sporocarps of Azolla Ⅱ effect of space conditions on the shade tolerance of different species of Azolla [J]. Acta Agriculturae Universitis Jiangxiensis, 2002, 24(6): 833−837.(in Chinese)
[23]	郭文杰, 鲁雪华, 林勇, 等. 满江红孢子果空间诱变效应的研究 Ⅰ. 高空条件对不同品系满江红孢子果生长发育的影响 [J]. 江西农业大学学报(自然科学), 2002, 24(4):489−492. GUO W J, LU X H, LIN Y, et al. A study on the effects of space induction of sporocarps of Azolla Ⅰ. effect of space condition on the growth and development of different Azolla strains [J]. Acta Agriculturae Universitis Jiangxiensis, 2002, 24(4): 489−492.(in Chinese)
[24]	郑伟文, 刘利华, 修文琼. 满江红鱼腥藻在羽叶满江红大孢子果内的去向及其超微结构的变化 [J]. 福建农业学报, 1998, 13(1):3−10. ZHENG W W, LIU L H, XIU W Q. The fate and ultrastructural changes of Anabaena azollae within the debeloping sporocarps of Azolla [J]. Fujian Journal of Agricultural Sciences, 1998, 13(1): 3−10.(in Chinese)
[25]	陈坚, 罗涛, 唐龙飞, 等. 三膘亚属满江红及其种间杂种雄性器官的数量分类研究初报 [J]. 福建省农科院学报, 1997, 12(4):42−46. CHEN J, LUO T, TANG L F, et al. A Study on quantitative classification for male reproductive organ of species and interspecific hybrids among Euazolla Subgenus [J]. Fujian Journal of Agricultural Sciences, 1997, 12(4): 42−46.(in Chinese)
[26]	唐龙飞, 陈坚, 章宁, 等. 小叶满江红与墨西哥满江红杂交后代有性器官(孢子果)形态学分析 [J]. 福建省农科院学报, 1996, 11(3):54−59. TANG L F, CHEN J, ZHANG N, et al. A morphological analysis on the sexual organ(sporocarps) of hybrid progenies between A. Microphylla and A. Mexicana [J]. Fujian Journal of Agricultural Sciences, 1996, 11(3): 54−59.(in Chinese)
[27]	郑伟文, 黄进华. 满江红结孢期间鱼腥藻侵染孢子果的新观察 [J]. 福建省农科院学报, 1994, 9(4):49−54. ZHENG W W, HUANG J H. New data on the lnfection of the developing sporocarps with the symbionts of Azolla during the sporulation [J]. Fujian Journal of Agricultural Sciences, 1994, 9(4): 49−54.(in Chinese)
[28]	ZHENG W W, LIN Y H, LIN Y H, et al. Electron microscopic observation of symbiotic relationship between Azolla and Anabaena during the megaspore germination and the sporeling development of Azolla [J]. Journal of Integrative Plant Biology, 1990, 32(7): 514−520, 585.
[29]	施燕如, 彭科林. 红萍孢子果出苗过程及出苗条件的研究 [J]. 湖南农业科技, 1980(1):46−49, 42. SHI Y R, PENG K L. Study on the seedling emergence process and seedling emergence conditions of the Azolla [J]. Hunan agricultural science, 1980(1): 46−49, 42.(in Chinese)
[30]	KAR P, MISHRA S, SINGH D. Azolla sporulation in response to application of some selected auxins and their combination with gibberellic acid [J]. Biology and Fertility of Soils, 2002, 35(5): 314−319. doi: 10.1007/s00374-002-0469-1