水稻褐飞虱若虫和成虫褪黑素含量测定及比较

施龙清; 解振兴; 姜照伟; 董萌; 占志雄

doi:10.19303/j.issn.1008-0384.2021.06.010

水稻褐飞虱若虫和成虫褪黑素含量测定及比较

doi: 10.19303/j.issn.1008-0384.2021.06.010

福建省农业科学院水稻研究所，福建　福州　350018

基金项目: 国家重点研发计划项目（2017YED0100100）；福建省科技计划公益类专项（2018R1021-4）；福建省农业科学院科研项目（DC2017-8）

详细信息

作者简介:
施龙清（1988−），男，博士，助理研究员，研究方向：农业害虫综合防治（shilongqing@faas.cn）

通讯作者:
占志雄（1964−），男，研究员，研究方向：农业害虫综合防治（zzx64@sohu.com）

中图分类号: S 433.3
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出版历程
- 收稿日期: 2020-12-17
- 修回日期: 2021-03-30
- 网络出版日期: 2021-07-13
- 刊出日期: 2021-06-28

HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages

Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350018, China

摘要

摘要: 目的明确褪黑素在褐飞虱各发育阶段的含量情况。方法通过高效液相色谱-串联质谱技术(HPLC-MS/MS)对1～5龄若虫和两种翅型雌、雄成虫体内褪黑素含量进行测定和比较。结果褐飞虱若虫期褪黑素含量在1～2龄若虫时期最低，平均为378.5 pg·g⁻¹。3龄若虫褪黑素含量最高，为856.6 pg·g⁻¹。4龄若虫褪黑素含量下降，为810.7 pg·g⁻¹，与3龄无显著差异。5龄若虫褪黑素含量下降显著，为574.5 pg·g⁻¹。褐飞虱成虫褪黑素含量与性别显著相关，但受翅型影响不明显。雌、雄成虫间的褪黑素水平有极显著差异，长、短翅雄性成虫的褪黑素含量分别为978.3 pg·g⁻¹和969.6 pg·g⁻¹，而长、短翅雌性的褪黑素含量仅为76.1 pg·g⁻¹和176.8 pg·g⁻¹，甚至低于1～2龄若虫时期。结论初步明确了褐飞虱各发育阶段体内褪黑素含量变化特点，为后续研究褐飞虱褪黑素功能及机理奠定基础。
- 褐飞虱 /
- 褪黑素 /
- 内源性激素 /
- 性别差异 /
- 水稻害虫 /
- 翅型
Abstract: Objective Melatonin contents in brown planthopper, Nilaparvata lugens (Stl), at various developmental stages were determined. Method HPLC-MS/MS was used to measure the melatonin contents in the 1^st to the 5^th instar nymphs as well as female and male macropterous and brachypterous adults of the insect. Result At nymph stage, N. lugens had the lowest average melatonin content of 378.5 pg·g⁻¹ in the 1^st and the 2^nd instars, the highest of 856.6 pg·g⁻¹ in the 3^rd instar, 810.7 pg·g⁻¹ in the 4^th instar, and 574.5 pg·g⁻¹ in the 5^th instar. Adult N. lugens differed significantly on melatonin contents between male and female but not between the macropterous and brachypterous wing types. The male of macropterous and brachypterous wing types contained 978.3 pg·g⁻¹ and 969.6 pg·g⁻¹ melatonin, respectively, which were extremely significantly higher than the female counterparts at 76.1 pg·g⁻¹ and 176.8 pg·g⁻¹, respectively. Conclusion The melatonin contents in N. lugens as nymphs and adults were determined.
- Nilaparvata lugens (Stål) /
- melatonin /
- endogenous hormone /
- sex difference /
- rice pest /
- wing type

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图 1 褪黑素标准曲线

Figure 1. Standard curve of melatonin

下载: 全尺寸图片幻灯片

图 2 褐飞虱1-5龄若虫褪黑素含量比较

注：图中小写字母标记不同者为差异显著（LSD, P＜0.05）。

Figure 2. Melatonin contents of 1^st to 5^th instar nymphs

Note: Data with different lowercase letters on same columns mean significant differences at 0.05 level ( LSD).

下载: 全尺寸图片幻灯片

图 3 褐飞虱成虫褪黑素含量比较

注：图中大写字母标记不同者为差异极显著（LSD, P＜0.01）。

Figure 3. Melatonin contents of adult N. lugens

Note: Data with different capital letters on same columns mean significant differences at 0.01 level (LSD).

下载: 全尺寸图片幻灯片

表 1 褪黑素质子化或去质子化的选择反应监测条件（[M+H]⁺或[M-H]⁻）

Table 1. Conditions for monitoring protonated [M+H]⁺ or deprotonated [M-H]⁻ melatonin reactions

物质名称 Compound name	极性 Polarity	母离子 Parent ion（m/z）	子离子 Daughter ion（m/z）	解簇电压 DP/ V	碰撞能量 CE/ V
Melatonin	+	233	174.1*/159.1/216.1	50	9/34/13
注：标记的为定量离子。 Note: Quantitative ion pair.

下载: 导出CSV

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