福建省七叶一枝花细菌性软腐病病原鉴定

郑梅霞; 苏海兰; 肖荣凤; 何桂强; 朱育菁

doi:10.19303/j.issn.1008-0384.2021.03.012

福建省七叶一枝花细菌性软腐病病原鉴定

doi: 10.19303/j.issn.1008-0384.2021.03.012

1.
福建省农业科学院农业生物资源研究所，福建福州 350003
2.
井冈山自然保护区长古岭林场，江西井冈山 343600

基金项目: 福建省科技计划公益类专项（2019R1034-9）；福建省农业科学院科技创新团队建设项目（STIT2017-2-8、kjfw20）；福建省农业科学院科技服务项目（2021KF21-1）；福建省科技厅对外合作项目（2020I0034）

详细信息

作者简介:
郑梅霞（1986−），女，硕士，助理研究员，研究方向：农业生物资源保护与利用（E-mail：zhengmeixia2005@163.com）

通讯作者:
朱育菁（1972−），女，博士，研究员，研究方向：农业生物资源保护与利用（E-mail：zyjingfz@163.com）

中图分类号: S 435
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出版历程
- 收稿日期: 2020-11-07
- 修回日期: 2020-12-19
- 网络出版日期: 2021-03-27
- 刊出日期: 2021-03-31

Pathogen of Bacterial Soft Rot on Paris polyphylla Smith var. chinensis (Franch.) Hara in Fujian

1.
Institute of Agricultural Bio-resources Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 　350003, China
2.
Changguling Forest Farm, Jinggangshan Nature Reserve, Jinggangshan, Jiangxi 　343600, China

摘要

摘要: 目的调查七叶一枝花软腐病的发病特点及危害，分离和鉴定七叶一枝花软腐病的病原菌，以期明确七叶一枝花软腐病的病原菌，为该病的大田防治提供依据。方法通过对七叶一枝花软腐病进行田间病害调查和采样，以及对采集来的七叶一枝花样品进行病原菌分离纯化、形态特征鉴定、16S rDNA基因序列特征及致病性测定，综合分析鉴定七叶一枝花软腐病的病原菌。结果七叶一枝花软腐病的田间病害调查结果表明从每年2月植株出苗开始，七叶一枝花叶片、茎秆和块茎均有软腐病发生，常发于通气不良、田里积水环境。叶片发病由初期受害部位的水浸状的坏死有刺激性气味产生，而后感病部位内部组织被分解为液体状态，直至软腐。对发病植株上分离出的菌株进行形态学、分子生物学特征和致病性测定，将七叶一枝花软腐病病原菌鉴定为胡萝卜软腐果胶杆菌Pectobacterium carowvorum。结论七叶一枝花在通气不良、排水不畅的环境中易感软腐病，胡萝卜软腐果胶杆菌P. carowvorum是七叶一枝花软腐病的病原菌。
- 七叶一枝花 /
- 软腐病 /
- 病原鉴定 /
- 胡萝卜软腐果胶杆菌
Abstract: Objective Characteristics and damages brought about by the soft rot on Paris polyphylla Smith var. chinensis (Franch.) Hara and the pathogen that caused the disease were studied for positive identification and field control. Method Specimens of the diseased plant tissues were collected for isolation, purification, morphological examination, 16S rDNA gene sequencing, and pathogenicity determination of the pathogen. Result The diseased leaves, stems, and tubers on the plants were usually found in the fields where air circulation and water drainage were poor. At onset of the disease, water-soaked necrosis and pungent odor began to appear on the infected plant parts. Then the internal tissues started to break down and liquify. The morphological, molecular biological characteristics, and pathogenicity on the isolate showed the responsible pathogen to be Pectobacterium carowvorum. Conclusion The soft rot disease on P. polyphylla was determined to be infected by P. carowvorum under poorly ventilated surrounding and inadequately drained soil in the field where the plants were cultivated.
- Paris polyphylla Smith var. chinensis (Franch.) Hara /
- soft rot disease /
- pathogen identification /
- Pectobacterium carowvorum

HTML全文

图 1 七叶一枝花植株软腐病发病症状

注：a：田间发病症状；b：叶部发病症状；c：茎秆部发病症状；d：块茎部发病症状。

Figure 1. Symptoms of soft rot disease on P. polyphylla

Note: a: Disease onset symptoms on plants in the field; b: Symptoms on leaves; c: Symptoms on stems; d: Symptoms on tubers.

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图 2 七叶一枝花植株人工接种致病症状.

注：a、b.对照正常植株和回接发病植株;c.茎秆涂抹回接发病症状。

Figure 2. Symptoms of soft rot disease on artificially inoculated P. polyphylla plant

Note: a and b: Reattached diseased and normal plants; c: Symptoms on reattached stalks smeared with disease.

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图 3 植株软腐病病原菌的菌落形态

Figure 3. Morphology of soft rot pathogen colonies

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图 4 植株软腐病病原细菌形态特征

Figure 4. Morphology of soft rot pathogen

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图 5 七叶一枝花植株软腐病病原细菌的发育树

Figure 5. Pathogenic bacteria development tree of soft rot disease on P. polyphylla

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