Abstract:
Objective Drought-tolerance at seed germination stage and genetic diversity of 56 mung bean varieties were determined for resource evaluation.
Method Selected mung bean seeds were treated with either water as control or 10% mannitol solution to simulate drought stress. Ten indicators on the draught-tolerance of the seeds at germination stage, such as relative germination rate and potential as well as root length of the sprouts, were monitored. The results were compared to categorize the germplasms using principal component, membership function, and multivariate analyses. Then, the amplified SSRs of the categorized germplasms provided the genetic basis for classifying the draught-resistant property of the seeds at germination stage.
Result The top 3 indicators of draught-tolerance comprised 57.87%, 16.42%, and 11.42% separately, and 85.71% accumulatively, in contrition from a principal component analysis. And, at the calculated comprehensive evaluation value D, two of the 56 germplasms were graded highly tolerant, 10 tolerant, 30 moderately tolerant, 13 susceptible, and 1 highly susceptible to drought. From the cluster analysis on the SSR data, the germplasms were classified into 25 of relatively drought-resistant, 26 of varying degrees of drought-resistant due to complex genetic properties, and 5 of drought-susceptible.
Conclusions The drought-tolerance of the 56 mung bean germplasms at seed germination stage and their genetic characteristics were determined and classified to facilitate future breeding and utilization of the resource.
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