Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets
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摘要:
目的 穗粒数的增加可以直接提高水稻的产量，簇生穗能增加穗粒数和着穗密度。为考察簇生稻品种内江P164与对照单粒稻品种9311中OsCl-6等穗发育及细胞周期相关基因的表达是否存在显著差异，探索通过调控品种中特定基因的表达量来指导水稻生产，为高产水稻品种选育提供理论依据。 方法 分别取种植于网室大田的簇生稻品种内江P164和对照单粒稻品种9311的幼穗进行RNA提取、qRT-PCR分析及相关数据分析。 结果 qRT-PCR结果显示，对于所选取的几个幼穗发育时期，OsCl-6的表达量在内江P164的幼穗中的表达量均高于9311。细胞周期依赖激酶类基因在9311中的表达高于内江P164，而B类细胞周期蛋白则在内江P164中的表达高于9311。Lax1、OsWOX3、RFL等穗发育相关基因在内江P164中的表达低于9311，而MFS1和CL-4的在内江P164中的表达则高于9311。 结论 簇生穗基因OsCl-6等穗发育相关基因及B类细胞周期蛋白在簇生稻品种内江P164与对照单粒稻品种9311中的表达存在显著差异，OsCl-6或通过表达量上调来抑制二次枝梗的发育等，将为深入挖掘穗发育相关基因之间的关系及簇生穗基因OsCl-6的分子机理研究提供参考。 Abstract:Objectives Expressions of the genes related to panicle development and cell cycle in the mutant rice with clustered spikelets were studied to pave the road for research with an attempt to increase number of grains per panicle on rice plant for improved yield. Methods The expressions of OsCl-6 and other panicle development-related genes as well as those associated with cell cycle in Neijiang P164 of the mutant variety that bears clustered spikelets on panicle were compared with those in the regular single-floret variety, 9311. Young panicles on Neijiang P164 and 9311 plants that were grown from the seeds in a netted greenhouse were collected for RNA extraction, qRT-PCR, and data analysis. Results The qRT-PCR results showed higher expression of OsCl-6 at different stages of panicle development in Neijiang P164 than 9311. The expressions of cell cycle dependent kinase genes in 9311 was higher than Neijiang P164, while those of class B cyclins in Neijiang P164 higher than 9311. Neijiang P164 had lower expressions on the panicle development-related genes such as LAX1 , WOX3, and RFL than did 9311, but higher on MFS1 and CL-4. Conclusions The significant differences in the expressions of the genes related to panicle development and cell cycle, including OsCl-6 and B-type cyclins, between Naijiang P164 and 9311 revealed a potential correlation between the genetic makeup and physiology of the two rice varieties. The upregulated OsCl-6 might inhibit the development of secondary branches on rice plants. As increased grains per panicle would directly boost the yield of a rice plant, clustered spikelets would mean improved crop yield. -
Key words:
- rice /
- clustered spikelets /
- panicle development /
- gene expression
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图 1 幼穗发育不同时期OsCl-6的表达量分析
Figure 1. Expression of OsCl-6 in young panicles at developmental stages
图 2 幼穗中细胞周期相关基因的表达分析
Figure 2. Expressions of cell cycle-related genes in young panicle
图 3 幼穗枝梗发育相关基因的表达分析
Figure 3. Expressions of panicle branch development-related genes in young panicle
表 1 9311和内江P164的农艺性状比较
Table 1. Agronomic characteristics of 9311 and Neijiang P164
品种
Variety株高
Plant
height/cm分蘖数
Tiller
number一次枝梗数
primary
branch number二次枝梗数
Secondary
branche number穗粒数
Spikelet number
per panicle穗长
Panicle
length/cm着粒密度
seed
density/10 cm花粉可育率
Fertility rate
of pollen/%9311 98.57 10.3 15.1 35.4 191.5 21.16 90.9 97.1 内江 P164
Neijiang P164125.24** 9.6 12.7* 21.0** 154.8** 23.42* 65.5** 96.4 注：*和**分别表示在0.05和0.01水平上差异显著。下同。
Note: * and **represent significant at 0.05 and 0.01 probability level, respectively. Same for the following.
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[1] 张现伟, 李经勇, 郑家奎, 等. 复粒稻种质资源及其遗传育种利用 [J]. 植物学报, 2013, 48(4):438−446.ZHANG X W, LI J Y, ZHENG J K, et al. Germplasm resources and genetic breeding utilization of multiple-grain rice [J]. Chinese Bulletin of Botany, 2013, 48(4): 438−446.(in Chinese) [2] 邱箭, 李勤, 李国林, 等. 复粒稻恢复系选育及利用初报 [J]. 中国种业, 2014(12):70−71.QIU J, LI Q, LI G L, et al. Breeding and utilization of restorer lines in multigrain rice [J]. China Seed Industry, 2014(12): 70−71.(in Chinese) [3] 周鹏, 邓其明, 涂诗航, 等. 水稻穗部簇生突变体(Cl-dz)的遗传分析与初步定位 [J]. 福建农业学报, 2012, 27(5):481−484. doi: 10.3969/j.issn.1008-0384.2012.05.006ZHOU P, DENG Q M, TU S H, et al. Genetic analysis and gene mapping of the clustered spikelets mutant (Cl-dz) of rice [J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 481−484.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.05.006 [4] 苏德文, 郑燕梅, 何炜, 等. 水稻簇生穗突变体基因研究进展 [J]. 福建农业学报, 2013, 28(9):931−937. doi: 10.3969/j.issn.1008-0384.2013.09.020SU D W, ZHENG Y M, HE W, et al. Progress on the gene of rice clustered spikelets (Cl) mutant [J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 931−937.(in Chinese) doi: 10.3969/j.issn.1008-0384.2013.09.020 [5] 陈光毅, 张红宇, 徐培洲, 等. 一个水稻簇生穗突变体的遗传分析与基因定位 [J]. 中国农学通报, 2014, 30(27):258−264. doi: 10.11924/j.issn.1000-6850.2014-0646CHEN G Y, ZHANG H Y, XU P Z, et al. Genetic analysis and gene mapping of a clustered spikelets mutant in rice [J]. Chinese Agricultural Science Bulletin, 2014, 30(27): 258−264.(in Chinese) doi: 10.11924/j.issn.1000-6850.2014-0646 [6] 姜玲, 姜宁, 曹黎明, 等. 水稻簇生穗基因CPB4的精细定位 [J]. 复旦学报(自然科学版), 2018, 57(6):757−766.JIANG L, JIANG N, CAO L M, et al. Fine-mapping CPB4 gene associated with clustered spikelets in rice [J]. Journal of Fudan University (Natural Science), 2018, 57(6): 757−766.(in Chinese) [7] ZHENG L Y, ZHU X D, QIAN Q, et al. Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets (Cl) mutant [J]. Chinese Science Bulletin, 2003, 48(6): 559−562. doi: 10.1360/03tb9119 [8] 龙小林, 向珣朝, 张艳丽, 等. 水稻簇生穗控制基因ts的定位及育种利用研究 [J]. 农业生物技术学报, 2014, 22(9):1123−1130. doi: 10.3969/j.issn.1674-7968.2014.09.009LONG X L, XIANG X C, ZHANG Y L, et al. Gene mapping and breeding utilization of rice (Oryza sativa L.) clustered spikelets control gene ts [J]. Journal of Agricultural Biotechnology, 2014, 22(9): 1123−1130.(in Chinese) doi: 10.3969/j.issn.1674-7968.2014.09.009 [9] JIANG G H, XIANG Y H, ZHAO J Y, et al. Regulation of inflorescence branch development in rice through a novel pathway involving the pentatricopeptide repeat protein sped1-D [J]. Genetics, 2014, 197(4): 1395−1407. doi: 10.1534/genetics.114.163931 [10] GUO M, YANG Y H, LIU M, et al. Clustered spikelets 4, encoding a putative cytochrome P450 protein CYP724B1, is essential for rice panicle development [J]. Chinese Science Bulletin, 2014, 59(31): 4050−4059. doi: 10.1007/s11434-014-0568-z [11] SONG S, WANG G F, HU Y, et al. OsMFT1 increases spikelets per panicle and delays heading date in rice by suppressing Ehd1, FZP and SEPALLATA-like genes [J]. Journal of Experimental Botany, 2018, 69(18): 4283−4293. doi: 10.1093/jxb/ery232 [12] ZHANG Z Y, LI J J, YAO G X, et al. Fine mapping and cloning of the grain number per-panicle gene (Gnp4) on chromosome 4 in rice (Oryza sativa L.) [J]. Journal of Integrative Agriculture, 2011, 10(12): 1825−1833. [13] QI P, LIN Y S, SONG X J, et al. The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1; 3 [J]. Cell Research, 2012, 22(12): 1666−1680. doi: 10.1038/cr.2012.151 [14] 宋建. 水稻细胞周期关键调节基因的全基因组分析及Orysa;DEL1功能的初步鉴定[D]. 泰安: 山东农业大学, 2008.SONG J. Genome-wide analysis of core cell cycle genes and function identification of Orysa; DEL1 in rice[D]. Tai’an: Shandong Agricultural University, 2008. [15] REN D Y, LI Y F, ZHAO F M, et al. MULTI-FLORET SPIKELET1, which encodes an AP2/ERF protein, determines spikelet meristem fate and sterile Lemma identity in rice [J]. Plant Physiology, 2013, 162(2): 872−884. doi: 10.1104/pp.113.216044 [16] BARROCO R M, PERES A, DROUAL A M, et al. The syclin-dependent kinase inhibitor Orysa; KRP1 plays an important role in seed development of rice [J]. Plant Physiology, 2006, 142(3): 1053−1064. doi: 10.1104/pp.106.087056 [17] WU Z G, JI J H, TANG D, et al. OsSDS is essential for DSB formation in rice meiosis [J]. Frontiers in Plant Science, 2015(6): 21. [18] 郑燕梅, 苏德文, 蒋家焕, 等. 水稻簇生穗基因OsCl-6的精细定位与候选基因分析 [J]. 农业生物技术学报, 2018, 26(7):1116−1123.ZHENG Y M, SU D W, JIANG J H, et al. Fine mapping and candidate gene analysis of clustered spikelet gene OsCl-6 in rice (Oryza sativa) [J]. Journal of Agricultural Biotechnology, 2018, 26(7): 1116−1123.(in Chinese) [19] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 [-Delta Delta C(T)] Method [J]. Methods (San Diego, Calif), 2001, 25(4): 402−408. doi: 10.1006/meth.2001.1262 [20] IKEDA K, SUNOHARA H, NAGATO Y. Developmental course of inflorescence and spikelet in Rice [J]. Breeding Science, 2004, 54: 147−156. doi: 10.1270/jsbbs.54.147 [21] 李经勇, 唐永群. 稗草DNA导入渝恢1351后代性状变异研究 [J]. 中国稻米, 2012, 18(2):27−29. doi: 10.3969/j.issn.1006-8082.2012.02.006LI J Y, TANG Y Q. Study on character variation of Panicum crus-galli DNA introduced Yuhui 1351 progeny [J]. China Rice, 2012, 18(2): 27−29.(in Chinese) doi: 10.3969/j.issn.1006-8082.2012.02.006 [22] 俞法明, 杨曙东, 王晓峰, 等. 顶小穗簇生型早籼稻新种质的创制与鉴定 [J]. 核农学报, 2019, 33(5):848−854. doi: 10.11869/j.issn.100-8551.2019.05.0848YU F M, YANG S D, WANG X F, et al. Creation and identification of new germplasm of early indica rice with top spikelet cluster [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(5): 848−854.(in Chinese) doi: 10.11869/j.issn.100-8551.2019.05.0848 [23] FOUAD A S, ALSOBEAI S M. In silico characterization of a cyclin dependent kinase-A (CDKA) and its coding gene in some Oryza species [J]. Baghdad Science Journal, 2020, 17(3): 0760. doi: 10.21123/bsj.2020.17.3.0760 -
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