鄂北岗地山药病害病原菌鉴定

杨小林; 张佑宏; 王佐乾; 常向前; 吕亮; 张舒; 杨新笋

doi:10.19303/j.issn.1008-0384.2021.01.008

鄂北岗地山药病害病原菌鉴定

doi: 10.19303/j.issn.1008-0384.2021.01.008

杨小林^{1, 2,},
张佑宏^3,,
王佐乾²,
常向前²,
吕亮²,
张舒^2, ,,
杨新笋^4, ,

1.
粮食作物种质创新与遗传改良湖北省重点实验室，湖北　武汉　430064
2.
湖北省农业科学院植保土肥研究所，湖北　武汉　430064
3.
湖北省生物农药工程研究中心，湖北　武汉　430064
4.
湖北省农业科学院粮食作物研究所，湖北　武汉　430064

基金项目: 粮食作物种质创新与遗传改良湖北省重点实验室基金项目（2019lzjj06）；国家重点研发计划项目（2018YFD0301305）

详细信息

作者简介:
杨小林（1971−），女，博士，研究员，研究方向：植物病原菌致病性及病虫害防控技术（E-mail：1205600908@qq.com）

共同第一作者：张佑宏（1970−），男，副研究员，研究方向：作物病虫害绿色防控技术（E-mail：2430204775@qq.com）

通讯作者:
张舒（1964－），男，研究员，研究方向：作物病虫害研究及防控（E-mail: zs6410@163.com）

杨新笋（1967－），男，博士，研究员，研究方向：鄂薯系列新品种的创制及应用（E-mail：yangxins@163.com）

中图分类号: S 432.1
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出版历程
- 收稿日期: 2020-07-30
- 修回日期: 2020-12-20
- 网络出版日期: 2021-02-08
- 刊出日期: 2021-01-31

Pathogens Infecting Chinese Yams Planted on Ridges in Northern Hubei

1.
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, Hubei 　430064, China
2.
Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 　430064, China
3.
Hubei Biological Pesticide Engineering Research Center, Wuhan, Hubei 　430064, China
4.
Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 　430064, China

摘要

摘要: 目的开展鄂北岗地山药病害病原菌的鉴定，探索引起病害的病原菌及其分类地位，为进一步研究其发生规律及制定切实有效的防治措施提供依据。方法采集鄂北岗地山药地上组织病害样本，利用植物病理学组织分离法和ITS-PCR技术，通过病原物培养形态、显微观察、ITS鉴定比对和致病性测定验证，进行山药病害病原菌的分离和鉴定。结果鄂北岗地山药病害的病原真菌系链格孢属互隔交链孢霉（Alternaria alternata），刺盘孢属果生炭疽菌（Colletotrichum fructicola）和胶孢炭疽菌（Colletotrichumn gloeosporiaides），毛色二孢属假可可毛色二孢（Lasiodiplodia pseudotheobromae）。结论以上结果揭示鄂北岗地山药有多病害发生，病原菌组成复杂。山药炭疽病及其他病害均可导致山药地上组织枯萎甚至死亡。
- 山药 /
- 病原菌 /
- 形态学 /
- ITS鉴定 /
- 刺盘孢属
Abstract: Objective Pathogens that cause serious diseases on Dioscorea batatas Decne grown on the mountain ridges in northern Hubei province were investigated to facilitate drafting effective disease prevention and control measures. Method Specimens of aboveground parts of the diseased plants were collected for pathogen isolation. The isolates were identified by colonial morphology, microstructure, and blasting sequence of ITS as well as the pathogenicity determination with tissue separation of phytopathology and ITS-PCR. Result The isolated pathogenic fungi included Alternaria alternate, Colletotrichum fructicola, C. gloeosporiaides, and Lasiodiplodia pseudotheobromae. Conclusion The pathogens causing the diseases, such as anthracnose, that can lead to withering or death of Chinese yams at the region were highly diversified and complex.
- Dioscorea batatas Decne /
- pathogen /
- morphology /
- ITS identification /
- Colletotrichum

HTML全文

图 1 山药病叶

Figure 1. Diseased leaves on vines of Chinese yam

下载: 全尺寸图片幻灯片

图 2 菌落形态

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 2. Colony morphology

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

图 3 显微形态

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 3. Microstructure

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

图 4 菌株基因组DNA-ITS

注：M: DL 2000 marker; 泳道1-4: 菌C20FG001～C20FG004

Figure 4. Applied fragment of pathogenic fungi by DNA-ITS

Note: M: DL 2000 marker; Lanes 1-4: fungi C20FG001-C20FG004.

下载: 全尺寸图片幻灯片

图 5 山药叶片接种菌株后的发病情况

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 5. Symptoms on leaves of Chinese yam plants after artificial fungal inoculation

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

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