小麦百农矮抗58幼苗对Na<sub>2</sub>SO<sub>4</sub>胁迫响应及阈值鉴定

刘铎; 白爽; 齐学斌; 宁东峰; 梁志杰; 郭魏; 李平

doi:10.19303/j.issn.1008-0384.2021.01.002

小麦百农矮抗58幼苗对Na₂SO₄胁迫响应及阈值鉴定

doi: 10.19303/j.issn.1008-0384.2021.01.002

刘铎^{1, 2,},
白爽³,
齐学斌^{1, 4},
宁东峰¹,
梁志杰^{1, 2, 4},
郭魏^{1, 4},
李平^{1, 4, ,}

1.
中国农业科学院农田灌溉研究所，河南　新乡　453002
2.
中国农业科学院农业水资源高效安全利用重点开放实验室，河南　新乡　453002
3.
北京市八大处公园管理处，北京 100041
4.
农业农村部农产品质量安全水环境因子风险评估实验室，河南　新乡　453002

基金项目: 中央级公益性行业科研院所基本科研业务费项目（FIRI2019-04-01）；国家重点研发计划支持项目（2017YFD0800403）；中国农业科学院科技创新工程项目（CAAS-ASTIP-FIRI-03）

详细信息

作者简介:
刘铎（1991−），男，博士，助理研究员，主要从事植物逆境生理生态研究（E-mail：ld258825@126.com）

通讯作者:
李平（1979−），男，博士，副研究员，主要从事农业资源环境研究（E-mail：firilp@163.com）

中图分类号: S 512
计量
- 文章访问数: 855
- HTML全文浏览量: 243
- PDF下载量: 29
- 被引次数: 0
出版历程
- 收稿日期: 2020-10-12
- 修回日期: 2020-12-10
- 网络出版日期: 2021-02-08
- 刊出日期: 2021-01-31

Response and Tolerance Threshold of Bainong Aikang-58 Wheat Seedlings toward Na₂SO₄ Stress

LIU Duo^{1, 2
,},
BAI Shuang³,
QI Xuebin^{1, 4},
NING Dongfeng¹,
LIANG Zhijie^{1, 2, 4},
GUO Wei^{1, 4},
LI Ping^{1, 4
, ,}

1.
Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang, Henan 　453002, China
2.
Key Laboratory of High-efficiency and Safe Utilization of Agriculture Water Resources of CAAS, Xinxiang, Henan 　453002, China
3.
Administering Department of Beijing Badachu Park, Beijing 　100041, China
4.
Water Environment Factor Risk Assessment Laboratory of Agricultural Products Quality and Safety, Ministry of Agriculture, Xinxiang, Henan 　453002, China

摘要

摘要: 目的百农矮抗58是我国小麦的主栽品种之一，探讨百农矮抗58幼苗对Na₂SO₄胁迫响应机制，并界定其对Na₂SO₄胁迫耐受阈值，进而为进一步深入研究提供基础数据，也为农业生产实践提供科学依据与理论指导。方法通过水培试验，设置不同浓度（0、20、40、60、80、100 mmol·L⁻¹）Na₂SO₄胁迫处理，通过测定株高、鲜重、生物量、各种抗氧化酶活性、质膜透性、丙二醛（MDA）含量及渗透调节物质含量，研究Na₂SO₄胁迫对小麦幼苗生长与生理的影响。结果随着Na₂SO₄浓度的升高，小麦幼苗的株高、鲜重及生物量均下降，小麦幼苗的各种抗氧化酶活性会随着Na₂SO₄浓度的升高而不断升高，当超过一定阈值后会下降，MDA含量和质膜透性会随着Na₂SO₄浓度的升高而升高，最大值分别为对照的3.20倍和4.94倍；小麦幼苗叶片的叶绿素含量随着Na₂SO₄浓度的升高而下降；小麦体内的可溶性糖含量随着Na₂SO₄浓度的升高呈先上升后下降的变化趋势，在80 mmol·L⁻¹时可溶性糖含量最高；脯氨酸含量则随着Na₂SO₄浓度的升高而升高，最大值为对照的8.51倍。结论根据试验结果界定小麦百农矮抗58耐Na₂SO₄胁迫阈值为80～100 mmol·L⁻¹。
- 盐碱地 /
- 矮抗58 /
- Na₂SO₄胁迫 /
- 阈值
Abstract: Objective Bainong Aikang-58 is one of the major wheat cultivars in China. To investigate the response mechanism of Bainon Aikang-58 seedling to Na₂SO₄stress was discussed and to define its tolerance threshold to Na₂SO₄ stress was determined so as to provide basic data support for further research and scientific basis and and theoretical guidance for agricultural production practice. Method Effects of Na₂SO₄ stress at 0, 20, 40, 60, 80, and 100 mmol·L⁻¹ on the growth and physiology of the wheat seedlings were studied in a hydroponic experimentation. Result As the Na₂SO₄ concentration in the medium increased, the plant height, fresh weight, and biomass of the seedlings decreased. The activity of each antioxidant enzyme in wheat seedlings would continuously increase with the increase of Na₂SO₄ concentration, and would decrease after exceeding a certain threshold. The membrane permeability and content of MDA in the synplasmic membrane increase with increasing of Na₂SO₄ concentration to maximize 3.2 and 4.94 times of the control. The chlorophyll content in the leaves were decreased with the increase of Na₂SO₄ concentration. The soluble sugar content in wheat increased first and then decreased with the increase of Na₂SO₄ concentration. The soluble sugar concentration was the highest at 80 mmol·L⁻¹Whereas, the proline content kept increasing to reach a maximum that was 8.51 times of control. Conclusion The tolerance threshold of Bainong Aikang-58 wheat seedling toward Na₂SO₄ stress was determined to be 80−100 mmol·L⁻¹.
- saline-alkali soil /
- Bainong Aikang-58 /
- Na₂SO₄ stress /
- tolerance threshold

HTML全文

图 1 Na₂SO₄胁迫对小麦幼苗生长影响

注：*代表与对照（CK）相比差异达到显著水平（P<0.05）,**代表与对照相比差异达到极显著水平（P<0.01），图2～4相同

Figure 1. Effect of Na₂SO₄ stress on growth of wheat seedslings

Note: * represents significant difference compared with control group (CK) (P < 0.05); ** represents extremely significant difference compared with control group (P < 0.01). Figures 2-4 were the same

下载: 全尺寸图片幻灯片

图 2 Na₂SO₄胁迫对小麦幼苗抗氧化系统影响

Figure 2. Effect of Na₂SO₄ stress on antioxidant system of wheat seedlings

下载: 全尺寸图片幻灯片

图 3 Na₂SO₄胁迫对小麦幼苗叶绿素含量的影响

Figure 3. Effect of Na₂SO₄ stress on chlorophyll content in leaves of wheat seedlings

下载: 全尺寸图片幻灯片

图 4 Na₂SO₄胁迫对小麦幼苗渗透调节物质含量的影响

Figure 4. Effect of Na₂SO₄ stress on content of osmosis regulators in wheat seedlings

下载: 全尺寸图片幻灯片

参考文献(39)

[1]	ZHANG G C, DAI L X, DING H, et al. Response and adaptation to the accumulation and distribution of photosynthetic product in peanut under salt stress [J]. Journal of Integrative Agriculture, 2020, 19(3): 690−699.
[2]	张金林, 李惠茹, 郭姝媛, 等. 高等植物适应盐逆境研究进展 [J]. 草业学报, 2015, 24(12):220−236. doi: 10.11686/cyxb2015233 ZHANG J L, LI H R, GUO S Y, et al. Research advances in higher plant adaptation to salt stress [J]. Acta Prataculturae Sinica, 2015, 24(12): 220−236.(in Chinese) doi: 10.11686/cyxb2015233
[3]	朱建峰, 崔振荣, 吴春红, 等. 我国盐碱地绿化研究进展与展望 [J]. 世界林业研究, 2018, 31(4):70−75. ZHU J F, CUI Z R, WU C H, et al. Research advances and prospect of saline and alkali Land creening in china [J]. World Forestry Research, 2018, 31(4): 70−75.(in Chinese)
[4]	郄金标, 张福锁, 田长彦. 新疆盐生植物[M]. 北京: 科学出版社, 2006.
[5]	徐恒刚. 中国盐生植被及盐渍化生态治理[M]. 北京: 中国农业科学技术出版社, 2004.
[6]	刘正祥, 张华新, 杨秀艳, 等. 植物对氯化钠和硫酸钠胁迫生理响应研究进展 [J]. 世界林业研究, 2015, 28(4):17−23. LIU Z X, ZHANG H X, YANG X Y, et al. Research progress on physiological responses of plants to NaCl and Na₂SO₄ stress [J]. World Forestry Research, 2015, 28(4): 17−23.(in Chinese)
[7]	LOUTFY N, SAKUMA Y, GUPTA D K, et al. Modifications of water status, growth rate and antioxidant system in two wheat cultivars as affected by salinity stress and salicylic acid [J]. Journal of Plant Research, 2020, 133(4): 549−570. doi: 10.1007/s10265-020-01196-x
[8]	马凤霞, 王沛, 张敏, 等. 叶面喷施硒肥对不同品种小麦产量及籽粒硒含量的影响 [J]. 山东农业大学学报(自然科学版), 2020, 51(1):25−30. MA F X, WANG P, ZHANG M, et al. Effect of selenium fertilizer spraying on wheat yield of different varieties and grain selenium content [J]. Journal of Shandong Agricultural University (Natural Science Edition), 2020, 51(1): 25−30.(in Chinese)
[9]	徐亚军, 赵龙飞, 邢鸿福, 等. 内生细菌对盐胁迫下小麦幼苗脯氨酸和丙二醛的影响 [J]. 生态学报, 2020(11):1−12. XU Y J, ZAO L F, XING H F, et al. Effects of endophytic bacteria on proline and malondialdehyde of wheat seedlings under salt stress [J]. ACTA Ecologica Sinica, 2020(11): 1−12.(in Chinese)
[10]	钮力亚, 王伟, 王伟伟, 等. 盐胁迫下小麦品种生理指标的变化规律 [J]. 中国农学通报, 2019, 35(2):1−4. doi: 10.11924/j.issn.1000-6850.casb17120087 NIU L Y, WANG W, WANG W W, et al. Physiological indexes of wheat varieties under salt stress: the change law [J]. Chinese Agicultural Science Bulletin, 2019, 35(2): 1−4.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb17120087
[11]	时丽冉, 白丽荣, 吕亚慈, 等. 小麦杂交品种衡9966苗期耐盐性分析 [J]. 作物杂志, 2018(6):149−153. SHI L R, BAI L R, LYU Y C, et al. Analysis of salt tolerance at the seedling stage of wheat hybrid vaiety heng 9966 [J]. Crops, 2018(6): 149−153.(in Chinese)
[12]	POUSTINI K, ESMAEILI A, ABBASI A, et al. Ion concentration and energy response of two wheat cultivars to salt stress [J]. Journal of Plant Nutrition, 2020, 43(1): 1−11.
[13]	XIE X, ZHAO F, ZHENG Y, et al. Identification of Tolerance of Wheat (Triticum Aestivum L.) With Different Ploidy under Salt Stress [J]. IOP Conference Series: Earth and Environmental ence, 2020, 598(1): 12074−12076.
[14]	KLAY I, RIAHI L, AMARA H S, et al. Genotypic variability for salt stress tolerance among wild and cultivated wheat germplasms at an early development stage [J]. Open Agriculture, 2019(4): 375−380.
[15]	WEI L, LV B, WANG M, et al. Priming effect of abscisic acid on alkaline stress tolerance in rice (Oryza sativa L.) seedlings [J]. Plant Physiology and Biochemistry, 2015, 90: 50−57. doi: 10.1016/j.plaphy.2015.03.002
[16]	王利军, 马履一, 王爽, 等. 水盐胁迫对沙枣幼苗叶绿素荧光参数和色素含量的影响 [J]. 西北农业学报, 2010, 19(12):122−127. doi: 10.3969/j.issn.1004-1389.2010.12.024 WANG L J, MA L Y, WANG S, et al. Effects of water ang salt stress on chlorophyll fluorescence parameters and pigment contents of Elaeagnus angustifolia L. seedlings [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2010, 19(12): 122−127.(in Chinese) doi: 10.3969/j.issn.1004-1389.2010.12.024
[17]	DIAO Q, SONG Y, QI H. Exogenous spermidine enhances chilling tolerance of tomato (Solanum lycopersicum L.) seedlings via involvement in polyamines metabolism and physiological parameter levels [J]. Acta Physiologiae Plantarum, 2015, 37(11): 230. doi: 10.1007/s11738-015-1980-y
[18]	BAO G, HE F, CHEN W, et al. Physiological effects of different concentrations of chloride deicing salt and freeze–thaw stress on Secale cereale L. seedlings [J]. Journal of Plant Growth Regulation, 2020, 39(1): 15−25. doi: 10.1007/s00344-019-09960-7
[19]	刘铎, 丛日春, 高卫东, 等. 盐碱胁迫对柳树抗氧化酶的影响 [J]. 水土保持通报, 2017, 37(5):53−57. LIU D, CONG R C, GAO W D, et al. Effects of salt and alkali stresses on antioxidases of willow [J]. Bulletin of Soil and Water Conservation, 2017, 37(5): 53−57.(in Chinese)
[20]	许迪, 李益农, 龚时宏, 等. 面向可持续灌溉农业发展的涝渍盐碱管理 [J]. 排灌机械工程学报, 2019, 37(1):63−72. XU D, LI Y N, GONG S H, et al. Waterlogging and saline-alkaline management for development of sustainably irrigated agriculture [J]. Journal of Drainage and Irrigation Machinery Engineering, 2019, 37(1): 63−72.(in Chinese)
[21]	王树凤, 胡韵雪, 孙海菁, 等. 盐胁迫对2种栎树苗期生长和根系生长发育的影响 [J]. 生态学报, 2014, 34(4):1021−1029. WANG S F, HU Y X, SUN H J, et al. Effects of salt stress on growth and root development of two oak seedlings [J]. Acta Ecolocica Sinica, 2014, 34(4): 1021−1029.(in Chinese)
[22]	孟祥浩, 林琪, 张玉梅, 等. 盐胁迫对小麦萌发的影响及耐盐指标的筛选 [J]. 华北农学报, 2014, 29(4):175−180. doi: 10.7668/hbnxb.2014.04.031 MENG X H, LIN Q, ZHANG Y M, et al. Effects of salt stress on germination of wheat and screening of salt tolerance indices [J]. Acta Agriculturae Boreali-Sinica, 2014, 29(4): 175−180.(in Chinese) doi: 10.7668/hbnxb.2014.04.031
[23]	GONG B, WEN D, VANDENLANGENBERG K, et al. Comparative effects of NaCl and NaHCO3 stress on photosynthetic parameters, nutrient metabolism, and the antioxidant system in tomato leaves [J]. Scientia Horticulturae, 2013, 157: 1−12.
[24]	BING-SHENG L, XIAO-WEI L, HONG-YUAN M, et al. Differences in Growth and Physiology of Rice in Response to Different Saline-Alkaline Stress Factors [J]. Agronomy Journal, 2013, 105(6): 1889. doi: 10.2134/agronj2013.0017er
[25]	朱金方, 刘京涛, 陆兆华, 等. 盐胁迫对中国柽柳幼苗生理特性的影响 [J]. 生态学报, 2015, 35(15):5140−5146. ZHU J F, LIU J T, LU Z H, et al. Effects of salt stress on physiology characteristics of Tamarix chinensis Lour. seedlings [J]. Acta Ecologica Sinica, 2015, 35(15): 5140−5146.(in Chinese)
[26]	LI H D, GAO H Y. Effects of different nitrogen application rate on allocation of photosynthetic electron flux in Rumex K-1 leaves [J]. Journal of Plant Physiology & Molecular Biology, 2007, 33(5): 417−424.
[27]	ZHANG Y, KAISER E, ZHANG Y, et al. Short-term salt stress strongly affects dynamic photosynthesis, but not steady-state photosynthesis, in tomato (Solanum lycopersicum) [J]. Environmental and Experimental Botany, 2018, 149: 109−119.
[28]	刘莉娜, 张卫强, 黄芳芳, 等. 盐胁迫对银叶树幼苗光合特性与叶绿素荧光参数的影响 [J]. 森林与环境学报, 2019, 39(6):601−607. LIU L N, ZHANG W Q, HUANG F F, et al. Effects of NaCl stress on the photosynthesis and cholorophyll fluorescence of heritiera littoralis seedlings [J]. Journal of Forest and Environment, 2019, 39(6): 601−607.(in Chinese)
[29]	王玥琳, 徐大平, 杨曾奖, 等. 移植和钾肥对降香黄檀光合特性与叶绿素含量的影响 [J]. 植物科学学报, 2018, 36(6):879−887. WANG Y L, XU D P, YANG Z J, et al. Effects of transplantation ang potassium fertilizer on the photosynthetic characteristics and chlorophyll content of dalbergia odorifera [J]. Plant Science Journal, 2018, 36(6): 879−887.(in Chinese)
[30]	于爽, 杨新宇, 高剑, 等. 盐碱胁迫对龙葵幼苗生长及叶绿素含量的影响 [J]. 贵州农业科学, 2018, 46(7):131−134. doi: 10.3969/j.issn.1001-3601.2018.07.033 YU S, YANG X Y, GAO J, et al. Effects of saline-alkali stress on growth and chlorophyll content of solanum nigrum seedlings [J]. Guizhou Agricultural Sciences, 2018, 46(7): 131−134.(in Chinese) doi: 10.3969/j.issn.1001-3601.2018.07.033
[31]	郑世英, 郑芳, 徐建, 等. 外源硅对NaCl胁迫下小麦幼苗生长及光合特性的影响 [J]. 麦类作物学报, 2015, 35(1):111−115. ZHENG S Y, ZHENG F, XU J, et al. Effects of silicon on the biomass and photosynthetic characteristics of wheat seedlings under NaCl stress [J]. Journal of Triticeae Crops, 2015, 35(1): 111−115.(in Chinese)
[32]	王康君, 樊继伟, 陈凤, 等. 植物对盐胁迫的响应及耐盐调控的研究进展 [J]. 江西农业学报, 2018, 30(12):31−40. WANG K J, FAN J W, CHEN F, et al. Reasearch advances in response of plants to salt stress and regulation of salinity tolerance [J]. Acta Agriculturae Jiangxi, 2018, 30(12): 31−40.(in Chinese)
[33]	王秀娟, 杨会青. 盐碱胁迫下西伯利亚白刺的渗透调节物质的变化 [J]. 热带农业科学, 2010, 30(2):34−36. doi: 10.3969/j.issn.1009-2196.2010.02.008 WANG X J, YANG H Q. Changes of osmosis-regulating substance of nitraria sibirica pall. under salt-alkali stress in Daqing [J]. Chinese Journal of Tropical Agriculture, 2010, 30(2): 34−36.(in Chinese) doi: 10.3969/j.issn.1009-2196.2010.02.008
[34]	李子英, 丛日春, 杨庆山, 等. 盐碱胁迫对柳树幼苗生长和渗透调节物质含量的影响 [J]. 生态学报, 2017, 37(24):8511−8517. LI Z Y, CONG R C, YANG Q S, et al. Effects of saline-alkali stress on growth and osmotic adjustment substances in willow seedlings [J]. Acta Ecologica Sinica, 2017, 37(24): 8511−8517.(in Chinese)
[35]	方志红, 董宽虎. NaCl胁迫对碱蒿可溶性糖和可溶性蛋白含量的影响 [J]. 中国农学通报, 2010, 26(16):147−149. FANG Z H, DONG K H. Effects of NaCl stress on soluble protein contents and soluble carbohydrate contents of artemisia cnethifolia [J]. Chinese Agricultural Science bulletin, 2010, 26(16): 147−149.(in Chinese)
[36]	杨颖丽, 李翠祥, 滕玉瑾, 等. NaCl胁迫下两种春小麦新品种渗透性调节反应的比较 [J]. 西北师范大学学报(自然科学版), 2016, 52(1):93−99. YANG Y L, LI C X, TENG Y J, et al. Comparison osmotic adjustment in two new spring wheat varieties under NaCl stress [J]. Journal of northwest normal university(Natural Science), 2016, 52(1): 93−99.(in Chinese)
[37]	代洪苇, 周盈盈, 郑姝婷, 等. 茶树脯氨酸转运蛋白基因鉴定及表达分析 [J]. 西北植物学报, 2020, 40(6):937−948. DAI H W, ZHOU Y Y, ZHENG S T, et al. Identification and expression profiling analysis of CsProTs gene family in tea plant [J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(6): 937−948.(in Chinese)
[38]	焦蓉, 刘好宝, 刘贯山, 等. 论脯氨酸累积与植物抗渗透胁迫 [J]. 中国农学通报, 2011, 27(7):216−221. JIAO R, LIU H B, LIU G S, et al. Discussion of accumulation of proline and Its relationship with osmotic stress tolerance of plants [J]. Chinese Agricultural Science Bulletin, 2011, 27(7): 216−221.(in Chinese)
[39]	周万海, 冯瑞章, 师尚礼, 等. NO对盐胁迫下苜蓿根系生长抑制及氧化损伤的缓解效应 [J]. 生态学报, 2015, 35(11):3606−3614. ZHOU W H, FENG R Z, SHI S L, et al. Nitric oxide protection of alfalfa seedling roots against salt-induced inhibition of growth and oxidative damage [J]. Acta Ecologica Sinica, 2015, 35(11): 3606−3614.(in Chinese)