福建省稻瘟病菌生理小种组成及水稻主栽品种的抗性筛选

邓云; 田大刚; 苏妍; 张洁薇; 吴建文

doi:10.19303/j.issn.1008-0384.2020.10.008

福建省稻瘟病菌生理小种组成及水稻主栽品种的抗性筛选

doi: 10.19303/j.issn.1008-0384.2020.10.008

1.
福建省南平市农业科学研究所，福建　南平　354200
2.
福建省农业科学院生物技术研究所，福建　福州　350003
3.
福建省南平生态与农业气象试验站，福建　南平　354200

基金项目: 福建省科技计划引导性项目（2017N0066）；农业农村部国家水稻产业技术体系—南平综合试验站项目（2016）

详细信息

作者简介:
邓云（1981−），女，硕士，高级农艺师，主要从事植物保护与抗病育种（E-mail: 25362663@qq.com）

中图分类号: S 435.111.4+1
计量
- 文章访问数: 953
- HTML全文浏览量: 427
- PDF下载量: 25
- 被引次数: 0
出版历程
- 收稿日期: 2019-08-30
- 修回日期: 2020-04-30
- 刊出日期: 2020-10-28

Physiological Races of Magnaporthe grisea and Disease-Resistant Rice in Fujian

1.
Nanping Institute of Agricultural Sciences, Nanping, Fujian　354200, China
2.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350001, China
3.
Nanping Ecological and Agrometeorological Experimentation Station, Nanping, Fujian　354200, China

摘要

摘要: 目的了解近年来福建省稻瘟病菌生理小种组成和福建省主栽水稻品种的稻瘟病抗性情况，可为合理布局抗病品种和稻瘟病抗性育种提供科学依据。方法于2017–2019年收集福建省17个县市（地区）的中稻稻瘟病穗颈瘟样本，分离单孢用7个中国鉴别品种进行分类鉴定，并从中筛选出60个福建省内具有代表性的稻瘟病菌株，室内喷雾接菌32个福建省主栽水稻品种，同时用9个稻瘟病抗性基因分子标记检测这32个主栽品种。结果 7个鉴别品种的鉴定分类结果显示，稻瘟病生理小种ZA群出现频率为39.48%，为优势种群；用60个代表性生理小种室内接菌鉴定结果显示，这些生理小种平均致病率为23.28%，相对较低，说明福建省推广的主栽水稻品种的抗瘟性相对较好；鉴定筛选出宽抗谱水稻品种15份，其中隆两优华占、两优332的抗谱最广，建议在生产上推广利用。结论福建省的稻瘟病生理小种已由早期的以ZB、ZC群为优势种群逐渐转变为以ZA群为优势种群，这与近年来甬优系列品种的大面积推广有关。在抗稻瘟病的品种布局上应优选抗谱宽度大的品种，同时在新品种推广种植中，应追溯宽抗谱水稻品种的致病生理小种来源地，尽量避开可能致使拟推广品种感病的生理小种所在地区。
- 稻瘟病 /
- 抗性鉴定 /
- 抗谱 /
- 抗性基因 /
- 分子标记
Abstract: Objective Physiological races of Magnaporthe grisea, the major pathogen of rice blast disease in Fujian in recent years, and the rice varieties resistant to the infection were studied to understand the fungal distribution and provide a clue for breeding to prevent the disease. Method Microbial samples from the diseased rice in 17 localities in the province were collected from 2017 to 2019. Monospores were isolated and classified by comparing with 7 species with known identifications. From them, 60 distinctive strains were sprayed indoor on 32 local rice varieties to detect the presence of 9 molecular markers of rice blast resistance genes on the inoculated rice plants. Result Using the 7 specimens with known identification and classification for comparison, the ZA group with an occurrence frequency at 39.48% was found to be the dominant physiological race of rice blast. Inoculation of the 60 physiological races on the 32 rice varieties showed an average pathogenicity rate of 23.28%. The relatively low infection rate suggested that most of the local rice were blast resistant to varying degrees. Subsequently, 15 of the rice varieties with broad resistance spectra were identified. Of which, Longliangyouhuazhan and Liangyou 332 exhibited the broadest spectra and were considered suitable for further applications. Conclusion The dominant physiological race of M. grisea appeared to have gradually changed over recent years from ZB and ZC to ZA group in Fujian. The transformation might relate to the large-scale promotion of the Yongyou rice series during the time. Consequently, it would be prudent in selecting rice varieties with a broad-spectrum on blast resistance for promotion. And, prior to introducing a new variety, an understanding of the origin of pathogenic physiological races would be necessary to preclude areas that are susceptible to the spread of the disease for the cultivation.
- Rice blast /
- resistance identification /
- resistance spectrum /
- resistance genes /
- molecular markers

HTML全文

图 1 60个稻瘟病菌株的聚类分析结果

Figure 1. Cluster analysis on 60 rice blast strains

下载: 全尺寸图片幻灯片

表 1 国际水稻研究所9级制标准

Table 1. International Rice Research Institute’s 9-level standards

级别 Level	表现 Expression
0	无病斑 No disease spots
1	叶片上产生针头状大小的褐点型病斑 Pinhead-sized brown spot-like disease spots are produced on leaves
2	稍大病斑 Slight serious disease spot
3	小圆形稍长的灰色病斑,边缘褐色,病斑直径1～2 mm Small round and slightly long gray lesion with brown edge and diameter of 1–2 mm
4	典型的纺锤形病斑, 长1～2 cm，通常局限在两条主脉间，危害面积不超过叶面积的2% A typical spindle-shaped lesion, 1–2 cm long, is usually confined between the two main veins, and its damage area does not exceed 2% of the leaf area.
5	典型病斑，危害面积不超过叶面积的10% Typical disease spots, the damage area does not exceed 10% of the leaf area
6	典型病斑，危害面积为叶面积的11%～25% Typical disease spots, the damage area is 11%–25% of the leaf area
7	危害面积为叶面积的26%～50% The damage area is 26%-50% of the leaf area
8	典型病斑，危害面积为叶面积的51%～75% Typical disease spots, the damage area is 51%–75% of the leaf area
9	典型病斑，危害面积为叶面积的76%至全叶枯死 Typical disease spots, the damage area is 76% of the leaf area to the whole leaf dead

下载: 导出CSV

表 2 32个福建省主栽水稻品种在接菌试验的抗瘟性鉴定结果

Table 2. Resistance of 32 local rice varieties inoculated with 60 physiological races of M. grisea

序号 Serial number	采集地 Collection area	生理小种 Physiological form	品种代号 Variety code
序号 Serial number	采集地 Collection area	生理小种 Physiological form	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32
1	光泽大陂 GuangzeDapi	ZC₁₅	R	R	R	M	R	R	R	R	R	R	R	R	R	R	R	R	R	M	S	S	R	R	R	R	M	R	R	R	R	R	R	R
2	光泽良种场 GuangzeLiangzhongchang	ZA₄₇	R	S	R	R	R	R	R	R	R	R	R	R	R	S	S	M	R	R	S	R	R	M	S	R	R	S	R	M	R	M	R	M
3	光泽良种场 GuangzeLiangzhongchang	ZA₃₉	R	R	R	R	R	R	R	S	R	R	R	S	R	S	S	R	R	R	S	R	R	M	S	R	R	S	S	R	R	R	R	S
4	建阳将口 JianyangJiangkou	ZC₈	R	S	R	R	R	R	R	R	R	R	R	R	M	S	R	R	R	M	S	R	R	S	M	M	R	R	R	R	R	R	R	R
5	建阳将口 JianyangJiangkou	ZA₃₉	M	S	R	R	R	R	R	M	R	R	M	R	M	S	S	R	R	M	S	R	R	R	S	R	R	M	R	R	R	M	R	M
6	建阳将口 JianyangJiangkou	ZB₇	M	S	R	R	R	R	R	M	R	R	S	R	S	S	S	R	R	R	S	R	R	S	S	R	M	M	M	R	R	R	R	M
7	建阳莒口 JianyangJukou	ZA₅	M	S	R	R	R	R	R	S	S	R	S	R	S	S	S	M	M	S	S	R	S	R	S	S	S	S	S	R	S	R	M	M
8	建阳莒口 JianyangJukou	ZA₅	M	R	R	M	R	R	R	R	R	R	R	M	R	S	R	R	R	R	M	R	R	S	R	R	R	R	R	R	R	R	R	R
9	建阳石宇 JianyangShiyu	ZD₄	R	R	R	R	R	R	R	M	R	M	R	M	R	M	S	R	R	R	S	R	R	S	S	S	R	R	R	R	R	R	R	R
10	建阳石宇 JianyangShiyu	ZD₆	R	M	R	M	R	R	R	R	R	R	R	M	R	R	R	R	R	R	S	R	M	R	R	R	R	M	R	R	R	R	R	R
11	建阳石宇 JianyangShiyu	ZA₅	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R
12	建阳石宇 JianyangShiyu	ZB₂	R	R	R	R	R	R	R	S	R	R	S	R	S	M	R	R	R	R	S	R	R	R	R	R	S	R	R	R	R	R	R	R
13	建阳石宇 JianyangShiyu	ZA₇	R	M	R	R	R	R	R	R	R	R	R	S	S	R	R	R	R	R	R	R	R	R	M	R	R	R	R	R	M	R	R	R
14	建阳石宇 JianyangShiyu	ZA₄₈	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	R	R	R	R
15	建阳石宇 JianyangShiyu	ZB₂	R	R	R	R	R	R	R	R	S	R	R	R	S	S	S	R	R	R	R	S	R	R	R	R	R	R	R	R	R	R	M	R
16	建阳石宇 JianyangShiyu	ZB₂	R	R	R	R	R	R	R	R	S	R	R	R	S	S	S	R	R	R	R	S	R	R	R	R	R	R	R	R	R	R	M	R
17	建阳石宇 JianyangShiyu	ZA₃₄	R	R	M	R	R	M	R	R	R	R	R	R	R	R	R	R	R	M	M	S	R	R	S	R	M	R	M	R	R	R	M	R
18	建阳童游 JianyangTongyou	ZB₅	R	S	S	S	R	R	R	R	S	R	S	R	S	S	S	S	R	R	S	S	S	S	S	R	S	S	S	S	S	R	S	S
19	建阳童游 JianyangTongyou	ZG₁	R	R	R	R	R	R	R	R	R	R	R	S	S	R	M	M	R	R	R	R	R	R	R	R	S	S	R	S	R	M	R	R
20	建阳童游 JianyangTongyou	ZG₁	R	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	S	R	R	R	R	R	R	R	M	R	R	R	R	R
21	龙岩上杭 LongyanShanghang	ZD₈	R	M	R	M	R	R	R	S	R	R	R	R	M	S	R	R	R	R	S	R	R	R	M	R	R	R	R	R	S	R	R	S
22	龙岩上杭 LongyanShanghang	ZA₆₄	S	S	R	R	R	R	S	R	S	R	M	M	S	S	R	R	R	M	S	R	R	R	R	R	S	R	R	R	R	R	R	R
23	龙岩上杭 LongyanShanghang	ZF₂	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	S	R	R	R	R	R	S	R	R	S
24	龙岩上杭 LongyanShanghang	ZG₁	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	M
25	浦城水北 PuchengShuibei	ZC₁₅	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	R	M	R	R	R	R	R	R	R	R	R	R	R	R	R
26	浦城水北 PuchengShuibei	ZD₇	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R	R	M	R	R	R	R
27	浦城水北 PuchengShuibei	ZB₉	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R	R	R	R	R	R	R
28	三明将乐 SanmingJiangle	ZB₃₂	R	R	R	R	R	R	R	R	R	R	R	R	R	M	M	R	R	R	S	R	R	R	R	R	R	R	R	R	R	R	R	R
29	三明将乐 SanmingJiangle	ZA₆	R	R	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	S	R
30	三明将乐 SanmingJiangle	ZA₅	R	R	M	R	M	R	R	R	M	R	S	R	S	S	S	R	R	R	S	R	S	R	S	R	S	R	R	R	S	R	R	R
31	三明将乐 SanmingJiangle	ZB₆	M	R	R	R	R	R	R	R	R	R	R	M	S	S	R	R	R	R	S	R	S	R	S	R	M	M	R	R	S	R	R	S
32	三明将乐 SanmingJiangle	ZA₁	R	R	R	R	R	R	R	S	R	R	R	R	S	R	R	S	R	R	S	R	S	R	S	R	S	R	R	R	S	R	R	S
33	三明将乐 SanmingJiangle	ZA₇	S	M	R	R	S	R	R	R	M	S	R	M	S	S	S	R	R	S	S	S	S	R	S	S	S	S	R	R	S	S	R	S
34	三明将乐 SanmingJiangle	ZD₇	M	R	R	M	R	M	R	M	R	M	R	M	S	S	M	M	R	M	S	M	S	R	S	M	S	M	R	S	S	M	R	S
35	三明将乐 SanmingJiangle	ZC₅	R	R	R	R	R	R	R	R	R	R	R	R	S	S	R	R	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	S
36	三明将乐 SanmingJiangle	ZD₇	R	R	R	R	R	R	R	R	R	R	R	S	R	S	R	M	R	R	S	R	R	R	S	R	R	R	R	R	S	R	R	R
37	三明将乐 SanmingJiangle	ZA₃₉	R	R	R	R	R	R	R	R	R	R	R	R	R	S	M	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R	R	S
38	三明宁化 ShanmingNinghua	ZA₅	R	R	R	S	R	R	R	R	M	R	S	R	M	S	S	R	R	R	S	R	M	M	S	R	R	R	M	R	R	R	R	M
39	三明宁化 ShanmingNinghua	ZA₆	R	R	S	S	M	M	M	M	R	R	R	M	S	R	R	R	R	S	S	S	R	R	M	R	S	S	M	R	R	R	R	R
40	三明宁化 ShanmingNinghua	ZD₇	S	R	S	R	M	R	R	S	R	M	R	R	S	S	R	R	R	R	R	R	S	R	S	S	S	S	R	R	R	S	M	S
41	三明宁化 ShanmingNinghua	ZC₇	R	S	R	M	R	R	M	M	S	S	S	R	S	S	S	M	R	R	M	R	S	R	S	R	S	S	S	R	S	R	M	S
42	三明宁化 ShanmingNinghua	ZC₅	R	R	R	R	R	R	M	S	R	R	R	S	M	S	R	R	R	S	R	R	R	R	R	R	R	M	M	R	R	M	R	M
43	三明宁化 ShanmingNinghua	ZB₅	R	R	R	R	R	R	R	R	R	R	R	R	S	R	M	R	R	R	M	R	R	R	R	R	S	S	R	R	R	R	R	R
44	三明泰宁 SanmingTaining	ZC₁₅	R	R	R	R	R	R	R	R	R	R	R	R	S	S	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	R	R	R
45	邵武大布干 ShaowuDabugan	ZC₃	R	R	R	R	R	R	R	R	R	R	M	R	R	R	S	M	R	R	S	R	R	R	R	R	R	R	R	R	R	R	R	R
46	邵武外南源 ShaowuWainanyuan	ZA₃₇	R	M	R	R	R	R	R	R	R	M	R	M	S	R	S	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	S
47	邵武外南源 ShaowuWainanyuan	ZA₁	R	S	S	M	R	M	M	S	S	S	S	R	S	S	S	S	M	M	S	R	S	R	S	M	S	S	S	R	S	R	M	S
48	邵武外南源 ShaowuWainanyuan	ZD₇	M	S	M	R	R	R	S	S	S	R	S	S	S	S	S	S	S	S	S	R	S	R	S	M	S	S	S	R	S	R	M	M
49	邵武外南源 ShaowuWainanyuan	ZD₈	R	R	R	R	R	R	R	R	R	R	M	R	R	S	M	R	R	R	S	R	M	R	R	R	R	M	S	R	R	R	R	M
50	松溪花桥 SongxiHuaqiao	ZA₅	R	M	R	R	M	R	R	R	R	R	R	R	R	M	R	R	R	R	R	R	R	R	R	R	R	R	R	S	M	R	R	R
51	松溪花桥 SongxiHuaqiao	ZA₅₅	R	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R	R	R	R	R	S	R	R	R	R	R	R	R
52	松溪花桥 SongxiHuaqiao	ZA₁	R	M	R	R	R	R	R	R	M	R	R	M	M	S	R	R	M	R	S	R	S	R	M	R	R	M	S	R	M	R	R	S
53	松溪花桥 SongxiHuaqiao	ZB₂₉	R	R	R	R	R	R	R	R	R	R	R	R	R	M	R	R	R	R	R	M	R	R	R	S	R	R	R	S	R	R	R	R
54	松溪祖墩 SongxiZudun	ZC₆	R	M	R	R	R	R	M	M	M	R	R	R	R	R	M	R	R	R	R	R	R	R	M	R	R	R	R	R	R	R	R	M
55	松溪祖墩 SongxiZudun	A₃₇	S	R	R	R	R	R	R	M	M	R	R	R	S	M	R	R	R	M	M	S	M	R	R	R	R	S	R	R	R	R	M	S
56	松溪祖墩 SongxiZudun	ZB₃₁	S	R	R	R	M	R	R	R	R	S	R	R	M	R	S	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R	R
57	松溪祖墩 SongxiZudun	ZC₁₃	R	R	S	R	R	R	S	M	S	M	S	R	R	S	S	R	R	R	S	R	S	R	S	R	S	S	R	R	S	R	R	R
58	武夷山五夫 WuyishanWufu	ZD₅	R	R	R	R	M	M	R	R	M	R	S	S	S	S	S	M	R	M	S	S	S	R	S	S	S	M	S	R	S	R	M	R
59	武夷山五夫 WuyishanWufu	ZA₇	R	R	R	M	R	R	R	M	R	R	R	R	S	S	S	R	R	M	S	R	R	R	S	R	R	R	M	R	M	R	R	R
60	武夷山五夫 WuyishanWufu	ZA₅	R	R	R	R	R	R	R	R	R	R	R	R	S	S	M	R	R	R	R	R	R	M	S	M	R	S	R	R	R	R	R	M
注：表中R表示水稻品种抗生理小种；M表示水稻品种中感生理小种；S表示水稻品种感病生理小种。 Note: R means resistant race of rice varieties; M means resistant race of rice varieties; S means susceptible race of rice varieties.

下载: 导出CSV

表 3 福建省60个稻瘟病菌株的致病率情况

Table 3. Pathogenicity of 60 rice blast strains from Fujian

序号 Serial number	地区 Area	生理小种 Physiological form	致病率 Pathogenicity/%	序号 Serial number	地区 Area	生理小种 Physiological form	致病率 Pathogenicity/%
1	光泽大陂 GuangzeDapi	ZC₁₅	10.94	31	三明将乐 ShanmingJiangle	ZA₇	48.44
2	光泽良种场 GuangzeLiangzhongchang	ZA₃₉	29.69	32	三明将乐 ShanmingJiangle	ZA₃₉	28.13
3	光泽良种场 GuangzeLiangzhongchang	ZA₄₇	26.56	33	三明将乐 ShanmingJiangle	ZB₆	28.13
4	建阳将口 JianyangJiangkou	ZA₃₉	29.69	34	三明将乐 ShanmingJiangle	ZB₃₂	9.38
5	建阳将口 JianyangJiangkou	ZB₇	34.38	35	三明将乐 ShanmingJiangle	ZC₅	17.19
6	建阳将口 JianyangJiangkou	ZC₈	18.75	36	三明将乐 ShanmingJiangle	ZD₇	12.5
7	建阳莒口 JianyangJiangkou	ZA₅	57.81	37	三明将乐 ShanmingJiangle	ZD₇	10.94
8	建阳莒口 JianyangJukou	ZA₅	12.5	38	三明宁化 ShanmingNinghua	ZA₅	35.94
9	建阳石宇 JianyangShiyu	ZA₅	1.56	39	三明宁化 ShanmingNinghua	ZA₆	42.19
10	建阳石宇 JianyangShiyu	ZA₇	10.94	40	三明宁化 ShanmingNinghua	ZB₅	7.81
11	建阳石宇 JianyangShiyu	ZA₃₄	17.19	41	三明宁化 ShanmingNinghua	ZC₅	12.5
12	建阳石宇 JianyangShiyu	ZA₄₈	1.56	42	三明宁化 ShanmingNinghua	ZC₇	21.88
13	建阳石宇 JianyangShiyu	ZB₂	17.19	43	三明宁化 ShanmingNinghua	ZD₇	53.13
14	建阳石宇 JianyangShiyu	ZB₂	17.19	44	三明泰宁 SanmingTaining	ZC₁₅	9.38
15	建阳石宇 Jianyangshiyu	ZB₂	17.19	45	邵武大布干 ShaowuDabugan	ZC₃	17.19
16	建阳石宇 JianyangShiyu	ZD₄	21.88	46	邵武外南源 haowuWainanyuan	ZA₁	67.19
17	建阳石宇 JianyangShiyu	ZD₆	10.94	47	邵武外南源 haowuWainanyuan	ZA₃₇	67.19
18	建阳童游 Jianyangtongyou	ZG₁	20.31	48	邵武外南源 haowuWainanyuan	ZD₇	17.19
19	建阳童游 JianyangTongyou	ZG₁	7.81	49	邵武外南源 haowuWainanyuan	ZD₈	9.38
20	建阳童游 JianyangTongyou	ZB₅	65.63	50	松溪花桥 SongxiHuaqiao	ZA₁	9.38
21	龙岩上杭 LongyanShanghang	ZA₆₄	29.69	51	松溪花桥 SongxiHuaqiao	ZA₅	6.25
22	龙岩上杭 LongyanShanghang	ZD₈	21.88	52	松溪花桥 SongxiHuaqiao	ZA₅₅	28.13
23	龙岩上杭 LongyanShanghang	ZF₂	10.94	53	松溪花桥 SongxiHuaqiao	ZB₂₉	10.94
24	龙岩上杭 LongyanShanghang	ZG₁	4.69	54	松溪祖墩 SongxiZudun	ZA₃₇	12.5
25	浦城水北 PuchengShuibei	ZC₁₅	3.13	55	松溪祖墩 SongxiZudun	ZB₃₁	40.63
26	浦城水北 PuchengShuibei	ZD₇	1.56	56	松溪祖墩 SongxiZudun	ZC₆	26.56
27	浦城水北 PuchengShuibei	ZD₈	3.13	57	松溪祖墩 SongxiZudun	ZC₁₃	51.56
28	三明将乐 ShanmingJiangle	ZA₁	57.81	58	武夷山五夫 WuyishanWufu	ZA₅	29.69
29	三明将乐 ShanmingJiangle	ZA₅	28.13	59	武夷山五夫 WuyishanWufu	ZA₇	18.75
30	三明将乐 ShanmingJiangle	ZA₆	32.81	60	武夷山五夫 WuyishanWufu	ZD₅	23.44

下载: 导出CSV

表 4 宽抗谱品种在福建省内避免推广的地区

Table 4. Areas in Fujian where rice varieties with a broad resistance spectrum should be avoided

序号 Serial number	品种 Variety	采集点内避免推广的地区 Areas within collection points to avoid promotion
1	和两优332 Heliangyou332	采集点范围内均适宜推广
2	隆两优华占 Longliangyouhuazhan	邵武外南源 Shaowu Wainanyuan
3	隆两优1988 Longliangyou 1988	三明将乐 SanmingJiangle
4	民优667 Mingyou667	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua
5	科优16 Keyou16	三明将乐 SanmingJiangle、建阳童游 JianyangTongyou
6	晶两优534 Jingliangyou534	龙岩上杭 LongyanShanghang、松溪祖墩 SongxiZudun、邵武外南源 Shaowuwainanyuan
7	福两优366 Fuliangyou366	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua、建阳童游 JianyangTongyou
8	隆两优锋占 Longyoufengzhan	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua、建阳童游 JianyangTongyou、邵武外南源 ShaowuWainanyuan
9	广优688 Guangyou688	三明将乐 SanmingJiangle、松溪华侨 SongxiHuaqiao、建阳童游 JianyangTongyou、邵武外南源 ShaowuWainanyuan
10	梦两优黄莉占 Mengliangyouhuanglizhan	三明宁化 SanmingNinghua、建阳童游 JianyangTongyou、松溪花桥 SongxiHuaqiao、邵武外南源 ShaowuWainanyuan
11	微两优898 Weiliangyou898	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua、松溪祖墩 SongxiZudun、邵武外南源 ShaowuWainanyuan、武夷山五夫 Wuyishanwufu
12	隆两优1377 Longliangyou1377	三明将乐 SanmingJiangle、龙岩上杭 LongyanShanghang、松溪祖墩 SongxiZudun、
13	晶两优华占 Jingliangyouhuazhan	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua、邵武外南源 ShaowuWainanyuan、建阳莒口 JianyangJukou
14	浙优18 Zheyou18	建阳童游 JianyangTongyou、建阳马伏 JianyangMafu、建阳石宇 JianyangShiyu、建阳将口 JianyangJiangkou
15	K两优369 Kliangyou369	三明将乐 SanmingJiangle、三明宁化 SanmingNinghua、松溪花桥 SongxiHuaqiao、松溪祖墩 SongxiZudun、建阳石宇 JianyangShiyu

下载: 导出CSV

表 5 宽抗谱水稻材料的抗谱及含有抗病基因的情况

Table 5. Resistance spectrum and resistance gene-containing status of rice varieties with broad resistance spectra

品种 Variety	抗谱 Resistance spectrum/%	抗性基因数 Number of disease resistance genes	含有的抗性基因 Contains resistance genes
和两优332 Heliangyou332	100.00	3	Pita、Pi25、Pi2
隆两优华占 Longliangyouhuazhan	98.33	5	Pita、Pi25、Pi5、Pib、Pi2
隆两优1988 Longliangyou 1988	98.33	5	Pita、Pi25、Pi5、Pib、Pi2
民优667 Mingyou667	96.67	1	Pi5
科优16 Keyou16	96.67	2	Pita、Pi5
晶两优534 Jingliangyou534	95.00	5	Pita、Pi25、Pi5、Pib、Pi2
福两优366 Fuliangyou366	95.00	3	Pita、Pi5、Pib
隆两优锋占 Longyoufengzhan	93.33	5	Pita、Pi25、Pi5、Pib、Pi2
广优688 Guangyou688	91.67	3	Pita、Pid3、Pi5
梦两优黄莉占 Mengliangyouhuanglizhan	91.67	2	Pi5、Pi2
微两优898 Weiliang898	91.67	4	Pi5、Pib、Pit、Pi2
隆两优1377 Longliangyou1377	91.67	5	Pita、Pi25、Pi5、Pib、Pi2
晶两优华占 Jingliangyouhuazhan	91.67	4	Pi25、Pi5、Pib、Pi2
浙优18 Zheyou18	91.67	4	Pita、Pid3、Pib、Piz-t
K两优369K liangyou369	90.00	4	Pita、Pi25、Pi5、Pi2

下载: 导出CSV

参考文献(22)

[1]	阮宏椿, 石妞妞, 杜宜新, 等. 水稻抗性基因Pi对福建省稻瘟病菌优势菌群的抗性分析 [J]. 中国水稻科学, 2017, 31(1):105−110. RUAN H C, SHI N N, DU Y X, et al. Analysis on resistance of pi genes to predominant races of mangnaporthe oryzae in Fujian Province, China [J]. Chinese Journal of Rice Science, 2017, 31(1): 105−110.(in Chinese)
[2]	OU S H. Pathogen variability and host resistance in rice blast disease [J]. Annual Review of Phytopathology, 1980, 18(1): 167−187. doi: 10.1146/annurev.py.18.090180.001123
[3]	辛威, 王敬国, 孙健, 等. 黑龙江省稻瘟病生理小种及品种资源抗性鉴定 [J]. 华北农学报, 2016, 31(4):130−137. doi: 10.7668/hbnxb.2016.04.022 XIN W, WANG J G, SUN J, et al. Physiological races of rice blast in Heilongjiang Province and species identification of resistance to resource [J]. Acta Agriculturae Boreali-Sinica, 2016, 31(4): 130−137.(in Chinese) doi: 10.7668/hbnxb.2016.04.022
[4]	邓云, 苏妍, 谢冬容, 等. 福建省稻瘟病生理小种及品种资源抗性鉴定 [J]. 福建稻麦科技, 2019, 37(1):12−15. DENG Y, SU Y, XIE D R, et al. Evaluation of the resistance of rice variety resources to blast pathogen population in Fujian [J]. Fujian Science and Technology of Rice and Wheat, 2019, 37(1): 12−15.(in Chinese)
[5]	王玲, 左示敏, 张亚芳, 等. 四川省稻瘟病菌群体遗传结构分析 [J]. 中国水稻科学, 2015, 29(3):327−334. doi: 10.3969/j.issn.1001-7216.2015.03.013 WANG L, ZUO S M, ZHANG Y F, et al. Genetic structure of rice blast pathogen Magnaporthe oryzae in Sichuan Province [J]. Chinese Journal of Rice Science, 2015, 29(3): 327−334.(in Chinese) doi: 10.3969/j.issn.1001-7216.2015.03.013
[6]	FLOR H H. Current status of the gene-for-gene concept [J]. Annual Review of Phytopathology, 1971, 9(1): 275−296. doi: 10.1146/annurev.py.09.090171.001423
[7]	张柱坚, 陈子强, 顾建强, 等. 稻瘟病抗性基因Pi-d2、Pi-d3和Pigm不同敲除突变体的抗性评价 [J]. 福建农业学报, 2018, 33(12):1231−1236. ZHANG Z J, CHEN Z Q, GU J Q, et al. Resistance on rice blast of knockout mutants of pi-d2, pi-d3 and pigm [J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1231−1236.(in Chinese)
[8]	杜宜新, 杨秀娟, 阮宏椿, 等. 福建省稻瘟病菌遗传谱系与致病型的关系 [J]. 福建农林大学学报(自然科学版), 2009, 38(2):124−128. DU Y X, YANG X J, RUAN H C, et al. Relationship between genetic lineages and pathotypes of Magnaporthe grisea isolates from Fujian [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2009, 38(2): 124−128.(in Chinese)
[9]	杨秀娟, 阮宏椿, 杜宜新, 等. 福建省稻瘟病菌致病性及其无毒基因分析 [J]. 植物保护学报, 2007, 34(4):337−342. doi: 10.3321/j.issn:0577-7518.2007.04.001 YANG X J, RUAN H C, DU Y X, et al. Pathogenicity and avirulence genes analysis of Magnaporthe grisea Barr. from rice in Fujian Province of China [J]. Acta Phytophylacica Sinica, 2007, 34(4): 337−342.(in Chinese) doi: 10.3321/j.issn:0577-7518.2007.04.001
[10]	肖丹凤, 张佩胜, 王玲, 等. 中国稻瘟病菌种群分布及优势生理小种的研究进展 [J]. 中国水稻科学, 2013, 27(3):312−320. doi: 10.3969/j.issn.1001-7216.2013.03.012 XIAO D F, ZHANG P S, WANG L, et al. Research progress on populations and physiological race distribution of rice blast pathogen(Magnaporthe grisea) in China [J]. Chinese Journal of Rice Science, 2013, 27(3): 312−320.(in Chinese) doi: 10.3969/j.issn.1001-7216.2013.03.012
[11]	鄂志国, 张丽靖, 焦桂爱, 等. 稻瘟病抗性基因的鉴定及其利用进展 [J]. 中国水稻科学, 2008, 22(5):533−540. doi: 10.3321/j.issn:1001-7216.2008.05.014 E Z G, ZHANG L J, JIAO G A, et al. Highlights in identification and application of resistance genes to rice blast [J]. Chin J Rice Sci, 2008, 22(5): 533−540.(in Chinese) doi: 10.3321/j.issn:1001-7216.2008.05.014
[12]	杜宜新, 李科, 阮宏椿, 等. 稻瘟病菌对稻瘟灵、异稻瘟净和三环唑的敏感性 [J]. 植物保护学报, 2011, 38(5):455−460. DU Y X, LI K, RUAN H C, et al. Sensitivities of Magnaporthe grisea to isprothiolane, iprobenfos and tricyclazole [J]. Acta Phytophylacica Sinica, 2011, 38(5): 455−460.(in Chinese)
[13]	全国稻瘟病菌生理小种联合试验组. 我国稻瘟病生理小种研究 [J]. 植物病理学报, 1980, 10(2):71−81. National Joint Test Group on physiological races of magnaporthe grisea. Studies on Physiological Races of Magnaporthe grisea in China [J]. Journal of Plant Pathology, 1980, 10(2): 71−81.(in Chinese)
[14]	陈福如, 阮宏椿, 杨秀娟, 等. 稻瘟病苗瘟叶瘟和穗颈瘟的相关性分析 [J]. 中国农学通报, 2006, 22(7):440−443. doi: 10.3969/j.issn.1000-6850.2006.07.112 CHEN F R, RUAN H C, YANG X J, et al. The correlation in seedling blasts, leaf blasts and neck blasts of rice [J]. Chinese Agricultural Science Bulletin, 2006, 22(7): 440−443.(in Chinese) doi: 10.3969/j.issn.1000-6850.2006.07.112
[15]	兰波, 杨迎青, 徐沛东, 等. 水稻主要抗瘟基因品系对江西省稻瘟病菌分离株系的抗性分析 [J]. 植物保护学报, 2014, 41(2):163−168. LAN B, YANG Y Q, XU P D, et al. Analysis of the resistance of rice major Pi-genes to the Magnaporthe oryzae isolates in Jiangxi Province [J]. Acta Phytophylacica Sinica, 2014, 41(2): 163−168.(in Chinese)
[16]	张国民, 马军韬, 肖佳雷, 等. 24个单基因系对黑龙江省优势菌群的抗性及联合抗病性分析 [J]. 中国农学通报, 2010, 26(12):233−237. ZHANG G M, MA J T, XIAO J L, et al. The blast resistance of 24 monogenic rice lines to prevalence physiologic races of Heilongjiang and analysis of pathogenicity association [J]. Chinese Agricultural Science Bulletin, 2010, 26(12): 233−237.(in Chinese)
[17]	张学博. 福建省稻瘟病菌生理小种研究的进展 [J]. 福建农学院学报, 1988, 17(4):361−367. ZHANG X B. Progress in the research on the physiological race of rice blast fungus in Fujian [J]. Journal of Fujian Agricultural College, 1988, 17(4): 361−367.(in Chinese)
[18]	张学博. 1986-1987年福建省的稻瘟病菌生理小种 [J]. 福建农业科技, 1990(6):6−7. ZHANG X B. Physiological race of the rice blast fungus in Fujian, China from 1986 to1987 [J]. Fujian Agricrltural Science and Technology, 1990(6): 6−7.(in Chinese)
[19]	黄志鹏, 张学博. 福建不同稻作类型稻瘟病菌生理小种研究 [J]. 福建农业大学学报, 1995, 24(1):39−44. HUANG Z P, ZHANG X B. Studies on the physiological race of rice blast fungus in different cropping take from Fujian [J]. Journal of Fujian Agricultural University, 1995, 24(1): 39−44.(in Chinese)
[20]	郑武, 阮志平, 鲁国东, 等. 1995-2000年福建省稻瘟病菌生理小种组成与分布动态 [J]. 福建农林大学学报(自然科学版), 2003, 33(1):46−49. ZHENG W, RUAN Z P, LU G D, et al. Composition and distribution dynamics of physiological races of rice blast fungus in Fujian Province from 1995 to 2000 [J]. Journal of Fujian Agricultural and Forestry University (Natural Science Edition), 2003, 33(1): 46−49.(in Chinese)
[21]	杨秀娟, 朱春雨, 阮宏椿, 等. 福建省稻瘟病菌毒性类型及部分水稻品种(组合)抗病性 [J]. 福建农林大学学报(自然科学版), 2008, 37(3):243−247. YANG X J, ZHU C Y, RUAN H C, et al. Pathogenic types of Magnaporthegrisea Barr. an the resistance of some rice cultivars to the pathogens in FuiJan Province [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2008, 37(3): 243−247.(in Chinese)
[22]	杜宜新, 李科, 石妞妞, 等. 2007-2009年福建省稻瘟病菌的生理小种变化研究 [J]. 福建农业学报, 2011, 26(2):275−279. doi: 10.3969/j.issn.1008-0384.2011.02.026 DU Y X, LI K, SHI N N, et al. Physiological race of Magnapor the grisea in Fujian from 2007 to 2009 [J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 275−279.(in Chinese) doi: 10.3969/j.issn.1008-0384.2011.02.026