Genetics of Combination Rice Hybrids at Heading Stage
-
摘要:
目的 水稻的抽穗期是一个复杂的遗传性状，其决定了水稻品种适应的生态区域，了解抽穗期的遗传基础，对水稻高产育种具有重要意义。 方法 以华占为研究材料，构建天优华占、恒丰优华占、五丰优华占3个杂交组合的P1、P2、F1、F2、BC1-1、BC1-2等6个遗传世代群体。运用植物数量性状主基因+多基因混合模型分析生育特性的遗传效应。 结果 3个杂交组合抽穗期的最适遗传模型均为MX1-AD-ADI。F2群体的主基因遗传率分别为10.13%、12.64%、13.65%，多基因遗传率分别为88.45%、85.94%、83.66%；BC1-1群体的主基因遗传率变幅为15.16%～23.66%，多基因遗传率变幅为65.33%～69.75%，抽穗期遗传模式与F2群体相似，主要受多基因控制；而以保持系为轮回亲本的3个回交群体BC1-2的抽穗期则受主基因和多基因共同影响，主基因遗传率变幅为42.03%～47.78%，多基因遗传率变幅为45.17%～50.77%。 结论 抽穗期受多基因控制，遗传基础复杂。在育种中的抽穗期选择上，应根据不同群体的遗传特性进行生育期的选择。 Abstract:Objective The very complex genetic characteristics at heading stage that dictate the adaptability of a rice variety to ecological zones were studied. Method Using Huazhan as male parent, populations of 6 generations (i.e., P1, P2, F1, F2, BC1-1, and BC1-2) of combination rice hybrids, Tianyou Huazhan (TH), Hengfengyou Huazhan (HH), and Wufengyou Huazhan (WH), were constructed. Genetic analysis on the heredity of plant height was performed using the "main gene+polygene hybrid genetic model" on the populations. Result MX1-AD-ADI was found to be the best fit genetic model for the heading of all 3 hybrids. On F2, the main genetic heritability of TH was 10.13%, that of HH 12.64%, and that of WH 13.65%; and, the polygenic heritability of TH was 88.45%, that of HH 85.94%, and that of WH 83.66%. For the BC1-1 population, the main gene heritability ranged from 15.16% to 23.66%, and the polygene from 65.33% to 69.75%. For the two populations, the genetic patterns at the heading stage were mainly controlled by the polygenes. But the heading of the 3 backcrossing BC1-2 populations with the maintainer as recurrent parent were affected by both the main genes and polygenes with the former exerting a heritability between 42.03% and 47.78 %, and the latter between 45.17% and 50.77%. Conclusion Rice heading was highly complicatedly controlled by multiple genes. In breeding, selection of heading period would, therefore, require a thorough analysis beforehand on the genetic characteristics of the specific target population. -
Key words:
- Rice (Oryza sativa L.) /
- heading stage /
- main genes+polygenic /
- genetic analysis
-
图 1 3个群体F1、F2、BC1-1、BC1-2的分离群体的播始天数频次分布
注：BC1-1为以华占为轮回亲本、BC1-2是以天丰B、恒丰B、五丰B作为轮回亲本
Figure 1. Frequency distribution of number of days from sowing to heading in 3 F1, F2, BC1-1, and BC1-2 populations
Note: metempsychosis parent of BC1-1 was Huachan; that of BC1-2 Tianfeng B, Hengfeng B, and Wufeng B.
表 1 3个杂交组合的6个遗传世代播抽天数田间表现（
$\scriptstyle\overline{\bf{X}}$ ±sd）Table 1. Heading days of 3 combination hybrids in 6 generations（
$\scriptstyle\overline{\bf{X}}$ ±sd）d 世代
For generations天优华占
Tianyou Hua zhan恒丰优华占
Hengfengyou Hua zhan五丰优华占
Wufengyou Hua zhanP1 81.53±0.51 81.53±0.51 81.53±0.51 P2 70.20±0.81 69.47±0.51 63.67±0.48 F1 80.77±1.25 75.73±0.91 77.30±0.70 F2 75.71±3.77 76.49±5.65 75.28±4.07 BC1-1 81.44±1.61 82.13±1.69 78.21±2.60 BC1-2 67.67±3.48 69.49±2.51 64.83±2.38 
下载: 导出CSV
表 2 3个杂交组合的抽穗期最优备选遗传模型分析
Table 2. Best fit genetic model for heading of 3 combination hybrids
性状
Shape and properties天优华占
Tianyou HuazhanSN 恒丰优华占
Hengfengyou HuazhanSN 五丰优华占
Wufengyou HuazhanSN 模型 model MLV AIC 模型 model MLV AIC 模型 model MLV AIC 抽穗期
heading periodPG-ADI −2293.65 4607.298 8 PG-ADI −2316.18 4652.356 8 MX2-ADI-AD −2189.39 4402.777 6 MX1-AD-ADI −2292.46 4608.923 7 MX1-AD-ADI −2314.23 4652.461 7 MX2-ADI-ADI −2186.06 4408.128 6 MX2-ADI-ADI −2289.97 4615.931 7 MX2-ADI-ADI −2310.75 4657.431 7 MX1-AD-ADI −2191.61 4413.215 5 注：SN表示遗传模型的适合性检验U12、U22、U32、nW2、Dn的统计量的显著个数, PG-ADI为加性−显性−上位性多基因遗传模型，MX1-AD-ADI为1对加性−显性主基因+加性−显性−上位性多基因遗传模型、MX2-ADI-ADI为2对加性−显性−上位性主基因+加性−显性−上位性多基因遗传模型、MX2-ADI-AD为2对加性−显性−上位性主基因+加性−显性多基因遗传模型。
Note: SN: statistic significant number for model fitting U12, U22, U32, nW2, and Dn; PG-ADI: additive−dominant−epigenetic polygenic genetic model; MX1-AD-ADI: pair of additive−dominant main genes+additive−dominant−epigenetic polygenic genetic model; MX2-ADI-ADI: 2 pairs of additive−dominant−epitropic major genes+additive−dominant−epigenetic multigene genetic model; MX2-ADI-AD: 2 pairs of additive−dominant−epitropic major genes+additive−dominant polygenic inheritance model.
下载: 导出CSV
表 3 3个杂交组合后代抽穗期的主基因和多基因遗传参数
Table 3. Parameters on main genes and polygenes in offspring of 3 combination hybrids at heading stage
参数值[计]
Parameter values参数
Parameter天优华占
Tianyou Hua zhan恒丰优华占
Hengfengyou Hua zhan五丰优华占
Wufengyou Hua zhan一阶参数估计值
1st order parameter estimatesm1 81.533 3 81.533 3 81.531 3 m2 70.201 5 69.471 6 63.670 8 a 2.295 5 2.556 6 2.094 4 d 1.008 6 1.117 3 1.531 3 d/a 0.439 4 0.437 0 0.731 1 二阶参数估计值
2nd-order parameter estimatesσmg2（B1） 0.432 0.534 6 1.601 3 hmg2（%） 15.160 0 18.759 9 23.655 6 σpg2（B1） 1.964 3 1.861 7 4.721 6 hpg2（%） 68.931 4 65.331 5 69.752 0 σmg2（B2） 2.644 3 3.230 7 3.025 3 hmg2（%） 42.026 6 45.846 5 47.781 8 σpg2（B2） 3.194 3 3.362 8 2.860 0 hpg2（%） 50.768 4 47.720 3 45.170 2 σmg2（F2） 3.229 6 4.027 5 2.261 1 hmg2（%） 10.132 5 12.635 8 13.653 σpg2（F2） 28.190 8 27.392 9 13.854 9 hpg2（%） 88.445 2 85.941 9 83.660 0 注：m1、m2分别为P1、P2的播始天数均值；a为1对主基因的加性效应值；d为1对主基因的显性效应值；
Note: m1 and m2 are mean values of P1 and P2 at heading stage, respectively; a is additive effect value of main gene; d is dominant effect value of main gene.
下载: 导出CSV
-
[1] 邹婉侬, 宋敏. 我国水稻育种成果的知识产权保护分析 [J]. 中国发明与专利, 2018, 15(7):12−14. doi: 10.3969/j.issn.1672-6081.2018.07.002ZOU W N, SONG M. Intellectual property protection analysis on intellectual property protection of rice breeding achievements in China [J]. Chinese Inventions and Patents, 2018, 15(7): 12−14.(in Chinese doi: 10.3969/j.issn.1672-6081.2018.07.002 [2] 赵礼才, 张玉娟. 水稻生产全程机械化发展现状、问题与思考 [J]. 农机使用与维修, 2019(4):80.ZHAO L C, ZHANG Y J. The present situation, problems and thoughts on the development of the whole mechanization of rice production [J]. Use and maintenance of agricultural machinery, 2019(4): 80.(in Chinese [3] 张祥喜, 汤洁, 王凤兰, 等. 几个籼型杂交水稻恢复系的抽穗期基因型诊断 [J]. 江西农业学报, 2005, 17(4):1−8. doi: 10.3969/j.issn.1001-8581.2005.04.001ZHANG X X, TANG J, WANG F L, et al. Genotype diagnosis of heading date in three restoring lines of Indica Hybrid rice (Oryza sativa) [J]. Acta Agriculturae Jiangxi, 2005, 17(4): 1−8.(in Chinese doi: 10.3969/j.issn.1001-8581.2005.04.001 [4] 胡时开, 苏岩, 叶卫军, 等. 水稻抽穗期遗传与分子调控机理研究进展 [J]. 中国水稻科学, 2012, 26(3):373−382.HU S K, SU Y, YE W J, et al. Advances in genetic analysis and molecular regulation mechanism of heading date in rice (Oryza sativa L.)/ [J]. Chinese Journal of Rice Science, 2012, 26(3): 373−382.(in Chinese [5] 任春元, 范淑秀, 刘进, 等. 不同年份水稻抽穗期和穗部性状QTL分析 [J]. 沈阳农业大学学报, 2017, 48(1):78−83.REN C Y, FAN S X, LIU J, et al. QTL analysis for heading stage and panicle traits at different years in rice [J]. Journal of Shenyang Agricultural University, 2017, 48(1): 78−83.(in Chinese [6] 李亚娟, 梁康迳, 王雪仁, 等. 籼型三系杂交稻生育期的遗传分析 [J]. 福建农林大学学报(自然科学版), 2003, 32(1):10−14.LI Y J, LIANG K J, WANG X R, et al. Genetic analysis of the growth stage in three-line indica hybrid rice [J]. Journal of Fujian Agricultural and Forestry University, 2003, 32(1): 10−14.(in Chinese [7] 曾晶, 姜恭好, 何予卿, 等. 利用籼粳交探讨水稻株高和抽穗期的遗传基础 [J]. 分子植物育种, 2006, 4(4):527−534. doi: 10.3969/j.issn.1672-416X.2006.04.013ZE NG, JIANG G H, HE Y Q, et al. The genetic bases analysis of plant height and heading date in lndica/Japonica hybrids [J]. Molecular Plant Breeding, 2006, 4(4): 527−534.(in Chinese doi: 10.3969/j.issn.1672-416X.2006.04.013 [8] 黄成, 姜树坤, 刘梦红, 等. 水稻抽穗期的QTL剖析 [J]. 华北农学报, 2009, 24(3):7−9. doi: 10.7668/hbnxb.2009.03.002HUANG C, JIANG S K, LIU M H, et al. QTL dissection of heading date in rice (Oryza sativa L. Japonica)/ [J]. Acta Agriculturae Boreali-Sinica, 2009, 24(3): 7−9.(in Chinese doi: 10.7668/hbnxb.2009.03.002 [9] 林成豹, 黄显波, 唐江霞, 等. 早熟恢复系DR02生育期特性的遗传分析 [J]. 福建农业学报, 2017, 32(5):495−497.LIN C B, HUANG X B, TANG J X, et al. Inheritance of a growth characteristic of precocious restorer rice line, DR02 [J]. Fujian Journal of Agricultural Sciences, 2017, 32(5): 495−497.(in Chinese [10] 郭梁, 张振华, 庄杰云. 水稻抽穗期QTL及其与产量性状遗传控制的关系 [J]. 中国水稻科学, 2012, 26(2):235−245. doi: 10.3969/j.issn.1001-7216.2012.02.014GUO L, ZHANG Z H, ZHUANG J Y. Quantitative trait loci for heading date and their relationship with the genetic control of yield traits in rice (Oryza sativa) [J]. Chinese Journal of Rice Science, 2012, 26(2): 235−245.(in Chinese doi: 10.3969/j.issn.1001-7216.2012.02.014 [11] 蒋丹, 洪广成, 陈倩, 等. 水稻抽穗期分子调控研究进展[J]. 水稻抽穗期分子调控研究进展[J]. 2019, 17(21): 7071-7077.JIANG D, HONG G C, CHEN Q, et al. Research progress in molecular regulation of heading date in rice(Oryza sativa)[J]. Molecular Plant Breeding, 2019, 17(21): 7071-7077. [12] 朱海霞, 王秋京, 闫平, 等. 孕穗抽穗期低温处理对黑龙江省主栽水稻品种结实率的影响 [J]. 中国农业气象, 2012, 33(2):304−309.ZHU H X, WANG Q J, YAN P, et al. Effect of low temperature at heading stage on seed setting rate of major rice varieties in Heilongjiang Province [J]. Chinese Journal of Agrometeorology, 2012, 33(2): 304−309.(in Chinese [13] 李黎红. 中国水稻研究所植物新品种权保护之实践 [J]. 中国种业, 2019(2):41−43.LI L H. Practice on the protection of new plant variety rights of China national rice research institute [J]. China Seed Industry, 2019(2): 41−43.(in Chinese [14] 盖钧镒, 章元明, 王建康. 植物数量性状遗传体系 [M]. 北京科学出版社, 2003. [15] 申聪聪, 朱亚军, 陈凯, 等. 利用水稻MAGIC群体关联定位抽穗期和株高QTL [J]. 作物学报, 2017, 43(11):1611−1621. doi: 10.3724/SP.J.1006.2017.01611SHEN C C, ZHU Y J, CHEN K, et al. Mapping of QTLs for heading date and plant height using MAGIC populations of rice [J]. Acta Agronomica Sinica, 2017, 43(11): 1611−1621.(in Chinese doi: 10.3724/SP.J.1006.2017.01611 [16] 邓晓建, 周开达, 李仁端, 等. 水稻品种生育期的遗传和基因定位 [J]. 四川农业大学学报, 2001, 19(2):172−178. doi: 10.3969/j.issn.1000-2650.2001.02.020DENG X J, ZHOU K D, LI R D, et al. Genetics and mapping of growth duration of rice varieties [J]. Journal of Sichuan Agricultural University, 2001, 19(2): 172−178.(in Chinese doi: 10.3969/j.issn.1000-2650.2001.02.020 [17] 申宗坦. 选育早熟矮秆水稻类型中一些性状的遗传分析 [J]. 作物学报, 1965, 4(4):391−401.SHEN Z T. Genetic analysis of some characters in breeding precocious dwarf rice [J]. Journal of Crops, 1965, 4(4): 391−401.(in Chinese [18] 李子先. 水稻抽穗期遗传变异的初步研究 [J]. 作物学报, 1980, 6(3):179−187.LI Z X. Preliminary study of genetic variation in rice heading stage [J]. Journal of Crops, 1980, 6(3): 179−187.(in Chinese [19] 陈爱华, 柳絮, 李广贤, 等. 水稻抽穗期基因上位性互作分析 [J]. 山东农业科学, 2012, 44(6):13−16. doi: 10.3969/j.issn.1001-4942.2012.06.003CHEN A H, LIU X, LI G X, et al. Analysis of epistatic interactions of genes for heading date in rice [J]. Shandong Agricultural Sciences, 2012, 44(6): 13−16.(in Chinese doi: 10.3969/j.issn.1001-4942.2012.06.003 [20] 张祥喜, 周开蓉, 汤洁, 等. 杂交水稻恢复系桂99的抽穗期基因及其效应分析 [J]. 作物学报, 2006, 32(6):833−839. doi: 10.3321/j.issn:0496-3490.2006.06.008ZHANG X X, ZHOU K R, TANG J, et al. Analysis of heading time gene and gene loci interactive effect in Gui99, a restorer line of hybrid rice (Oryza sativa L.)/ [J]. Acta Agronomica Sinica, 2006, 32(6): 833−839.(in Chinese doi: 10.3321/j.issn:0496-3490.2006.06.008 -
点击查看大图
图(1) / 表(3)
计量
- 文章访问数: 1314
- HTML全文浏览量: 348
- PDF下载量: 34
- 被引次数: 0
