外源GB对盐胁迫下2种黄芪种子萌发及幼苗抗氧化酶的影响

韩多红; 王恩军; 张勇

doi:10.19303/j.issn.1008-0384.2020.08.012

外源GB对盐胁迫下2种黄芪种子萌发及幼苗抗氧化酶的影响

doi: 10.19303/j.issn.1008-0384.2020.08.012

韩多红^{1, 2,},
王恩军²,
张勇²

1.
河西学院生命科学与工程学院，甘肃　张掖　734000
2.
甘肃省河西走廊特色资源利用省级重点实验室，甘肃　张掖　734000

基金项目: 全国第四次中药资源普查项目（GSZYPC201815）；甘肃省河西走廊特色资源利用重点实验室面上项目（XZ1405）；甘肃省高校产业支撑引导项目（2019C-01）

详细信息

作者简介:
韩多红（1977−），男，副教授，研究方向：中药材栽培及药用植物资源利用（E-mail：handuohong@163.com）

中图分类号: S 567.1
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出版历程
- 收稿日期: 2019-12-06
- 修回日期: 2020-07-03
- 刊出日期: 2020-08-19

Effect of Glycine Betaine on Seed Germination and Antioxidant Enzymes in Seedlings of Astragalus membranaceus under Salt Stress

1.
College of life sciences and engineering, Hexi University, Zhangye, Gansu　734000, China
2.
Key Provincial Laboratory of Universities in Gansu Province for Characteristic Resources Utility in Hexi Corridor, Zhangye, Gansu　734000, China

摘要

摘要: 目的探讨外源甜菜碱（Glycinebetaine,GB）对缓解盐胁迫下2 种黄芪种子及幼苗的作用机理。方法对受盐胁迫（100 mmol·L⁻¹ NaCl）的蒙古黄芪和膜荚黄芪的种子及幼苗进行施加不同浓度（0 ～40 mmol·L⁻¹）外源GB处理，测定不同处理后种子的发芽势、发芽率、发芽指数、活力指数和相对盐害率，以及幼苗SOD、CAT、POD、APX、GR和DHAR的活性。结果盐胁迫会抑制2 种黄芪种子的萌发，30 mmol·L⁻¹外源GB处理的蒙古黄芪和膜荚黄芪分别比盐胁迫对照组（100 mmol·L⁻¹ NaCl，CK₂）的发芽率提高159.07 %和167.01 %，相对盐害率降低74.38 %和76.18 %。用10～30 mmol·L⁻¹外源GB处理盐胁迫下的黄芪幼苗，可提高SOD、CAT和POD活性，增强APX、GR和DHAR活性，但不同品种存在差异，膜荚黄芪增加幅度大于蒙古黄芪。结论 30 mmol·L⁻¹的外源GB能有效缓解盐胁迫对黄芪植株的伤害，品种间存在差异，膜荚黄芪的耐盐性及对外源GB的生理响应大于蒙古黄芪。
- 甜菜碱 /
- 蒙古黄芪 /
- 膜荚黄芪 /
- 盐胁迫 /
- 抗氧化酶
Abstract: Objective Effects of exogenous treatment of glycine betaine (GB) on seeds and seedlings of Astragalus membranaceus under salt stress was studied to decipher the stress alleviation. Method The seeds and seedlings of A. membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao (Am H) and A. membranaceus (Fisch.) Bge. (Am B) under 100 mmol·L⁻¹ NaCl stress were treated with GB 0–40 mmol·L⁻¹. The rate, potential, and index of seed germination as well as the seedling vigor index and relative salt damage occurrence percentage to the plants were determined. The activities of SOD, CAT, POD, APX, GR and DHAR in the seedlings were analyzed. Result Addition of GB 30 mmol·L⁻¹ to the salt containing medium increased the germination rate of Am H seeds by 159.07% and of Am B seeds by 167.01%, while reduced the relative rate of salt damage on Am H seedlings by 74.38% and on Am B seedlings by 76.18% in comparison to those without the addition. Under salt stress, the seedlings treated with GB 10–30 mmol·L⁻¹ raised on the SOD, CAT, POD, APX, GR and DHAR activities in varying degrees. In general, the activity increases were greater on seedlings of Am B than those of Am H. Conclusion The addition of GB 30 mmol·L⁻¹ effectively ameliorated the adverse effects of the salt stress on the seeds and seedlings of A. membranaceus and more pronounced on Am B than on Am H.
- glycine betaine /
- Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao /
- Astragalus membranaceus (Fisch.) Bge. /
- salt stress /
- antioxidant enzyme activity

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图 1 外源GB对盐胁迫下黄芪幼苗SOD、POD和CAT活性的影响

注：图柱上方同一个种不同处理间无相同小写字母表示在0.05水平上差异显著（P＜0.05），同一个种不同处理间有相同小写字母表示在0.05水平上差异不显著（P＞0.05），图2同。

Figure 1. Effects of GB on SOD, POD and CAT activities in A. membranaceus seedlings under salt stress

Note: The absence of the same lowercase letter in the same treatment above the figure column indicates a significant difference at the 0.05 level（P＜0.05）, while the presence of the same lowercase letter in the same treatment indicates no significant difference at the 0.05 level（P＞0.05）, as shown in Fig 2.

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图 2 外源GB对盐胁迫下黄芪幼苗APX、GR和DHAR活性的影响

Figure 2. Effects of GB on APX, GR and DHAR activities in A. membranaceus seedlings under salt stress

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表 1 外源GB对盐胁迫下黄芪种子萌发的影响

Table 1. Effect of GB on seed germination of A. membranaceus under salt stress

种质材料 Germplasm Materials	处理 Treatment	发芽率 Germination rate/%	发芽势 Germination vigor/%	发芽指数 Germination index	活力指数 Vigor index	相对盐害率 Relative salt damage rate/%
蒙古黄芪 Astragalus membranaceus（Fisch.）Bge. var. mongholicus（Bge.）Hsiao	CK₁	67.78 ± 2.22 a	44.13 ± 1.79 a	9.06 ± 0.10 a	0.59 ± 0.08 a	0.00 ± 0.00 f
	CK₂	23.33 ± 1.93 f	8.26 ± 0.21 e	2.79 ± 0.13 f	0.13 ± 0.01 c	65.33 ± 3.97 a
	T₁	30.00 ± 1.92 e	15.56 ± 1.11 d	4.26 ± 0.20 e	0.17 ± 0.00 c	52.14 ± 2.82 b
	T₂	48.22 ± 0.29 c	32.67 ± 2.04 c	5.63 ± 0.17 c	0.26 ± 0.07 bc	30.54 ± 0.93 d
	T₃	60.44 ± 1.55 b	36.45 ± 0.12 b	8.07 ± 0.19 b	0.37 ± 0.01 b	16.74 ± 1.80 e
	T₄	33.22 ± 0.59 d	17.56 ± 0.59 d	4.90 ± 0.04 d	0.24 ± 0.01 bc	40.54 ± 2.02 c
膜荚黄芪 Astraglus membranaceus（Fisch.）Bge.	CK₁	62.65 ± 2.48 a	41.22 ± 0.02 a	8.83 ± 0.27 a	0.52 ± 0.04 a	0.00 ± 0.00 e
	CK₂	22.24 ± 0.11 e	7.35 ± 0.16 e	2.30 ± 0.22 e	0.11 ± 0.02 d	62.21 ± 1.08 a
	T₁	28.08 ± 2.10 d	14.45 ± 0.56 d	4.09 ± 0.12 d	0.14 ± 0.05 cd	55.18 ± 2.92 b
	T₂	42.66 ± 2.23 c	29.08 ± 2.03 c	5.38 ± 0.09 c	0.23 ± 0.00 c	34.92 ± 2.59 c
	T₃	59.38 ± 2.98 b	34.25 ± 2.24 b	7.13 ± 0.49 b	0.33 ± 0.03 b	14.82 ± 1.65 d
	T₄	33.61 ± 2.15 d	15.79 ± 0.40 d	4.52 ± 0.22 d	0.21 ± 0.03 cd	45.97 ± 5.23 bc
注：表中数值为平均数±标准差，同列数据中无相同小写字母表示处理间差异显著（P＜0.05）。 Note: Data are mean±standard error; those with different lowercase letters on same column indicate significant differences between treatments (P＜0.05). Different lowercase letters on top of column indicate significant difference, while same lowercase letter no significant difference (P＞0.05). Same for Fig. 2.

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