盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响

张亮; 李玉婷; 夏文静; 许晓风

doi:10.19303/j.issn.1008-0384.2020.08.011

盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响

doi: 10.19303/j.issn.1008-0384.2020.08.011

张亮^1,,
李玉婷¹,
夏文静¹,
许晓风^{1, 2, ,}

1.
南京师范大学泰州学院化学与生物工程学院，江苏泰州　225300
2.
南京师范大学生命科学学院，江苏南京　210023

基金项目: 江苏省高等学校自然科学研究面上项目（2020）；江苏省高校实验室研究会立项课题（GS2019YB06）

详细信息

作者简介:
张亮（1987−），男，硕士，讲师，研究方向：植物逆境生理与植物营养（E-mail：liangzai0061@126.com）

通讯作者:
许晓风（1959−），男，博士，教授，研究方向：植物生理与分子生物学（E-mail：xuxiaofeng@njnu.edu.cn）

中图分类号: Q 945.78
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出版历程
- 收稿日期: 2019-11-08
- 修回日期: 2020-05-05
- 刊出日期: 2020-08-19

Effect of Pre-soaking Seeds in Isosteviol Solution on Germination and Seedling Growth of Brassica napus under Salt Stress

ZHANG Liang^1
,,
LI Yuting¹,
XIA Wenjing¹,
XU Xiaofeng^{1, 2
, ,}

1.
School of Chemistry and Bioengineering, Taizhou College, Nanjing Normal University, Taizhou, Jiangsu　225300, China
2.
College of Life Science, Nanjing Normal University, Nanjing, Jiangsu　210023, China

摘要

摘要: 目的探索异甜菊醇浸种对盐胁迫下油菜种子发芽及幼苗生长的影响。方法以秦油2号油菜种子为材料，在盐胁迫下添加不同浓度的异甜菊醇，对其进行浸种处理。统计油菜种子的发芽率、发芽指数和活力指数，测定幼苗苗高、根长、叶绿素含量、根系活力、丙二醛（MDA）含量及抗氧化酶活性等生理指标。结果单纯盐胁迫下，油菜种子的发芽力受到抑制，种子发芽指标显著减低，NaCl敏感浓度为140 mmol·L⁻¹；1×10⁻⁸ mol·L⁻¹异甜菊醇浸种处理可显著增加盐胁迫下油菜幼苗的根系活力、子叶中叶绿素总量和SOD活性，分别比CK₂（去离子水浸种、140 mmol·L⁻¹NaCl胁迫）增加27.33%、36.94%和83.31%；显著降低幼苗子叶的MDA的含量，比CK₂降低32.11%。而1×10⁻⁹ mol·L⁻¹异甜菊醇浸种处理可显著增加油菜幼苗子叶的POD和CAT活性，分别比CK₂增加140.80%和47.25%。异甜菊醇浸种还可显著提高油菜种子的发芽率、发芽指数和活力指数，显著增加幼苗的苗高和根长。结论用适宜浓度（1×10⁻⁹～1×10⁻⁸ mol·L⁻¹）的异甜菊醇浸种可有效增强油菜幼苗的抗盐能力，缓解盐胁迫对种子发芽和幼苗生长造成的伤害。
- 异甜菊醇 /
- 油菜 /
- 盐胁迫 /
- 种子萌发 /
- 抗氧化酶
Abstract: Objective Effects of pre-soaking seeds in isosteviol solution on germination and seedling growth of Brassica napus under salt stress were studied. Method Seeds of Qinyou No. 2, a variety of B. napus , were pre-soaked in isosteviol solutions of different concentrations to determine the rate, index, and vigor of the seeds, as well as the height, chlorophyll content, root length and activity, malonaldehyde (MDA) content, and antioxidant enzyme activity of the seedlings under salt stress. Result Salt stress inhibited the germination of B. napus seeds with a threshold concentration at NaCl 140 mmol ·L⁻¹. By pre-soaking the seeds in a 1×10⁻⁸ mol·L⁻¹ isosteviol solution, the root activity, chlorophyll content, and SOD activity in cotyledons of the seedlings were significantly increased. Compared to CK₂ (seeds pre-soaked in deionized water prior to germination in a NaCl 140 mmol ·L⁻¹ medium), the isosteviol treatment increased 27.33% on root activity, 36.94% on chlorophyll content, and 83.31% on SOD activity, while significantly reduced MDA content by 32.11% in the cotyledons. When a 1×10⁻⁹ mol·L⁻¹ isosteviol solution was used for the pre-soaking, the POD and CAT activities were significantly increased by 140.80% and 47.25%, respectively, over those under CK₂. The pre-soaking treatment also significantly improved the germination rate, index, and vigor of the seeds, and significantly increased the height and root length of the seedlings. Conclusion Pre-soaking the seeds with an isosteviol solution of appropriate concentration (i.e., 1×10⁻⁹−1×10⁻⁸ mol·L⁻¹) enhanced the salt resistance of B. napus reducing the adverse effects on the seed germination and seedling growth caused by the salt stress.
- isosteviol /
- Brassica napus /
- salt stress /
- seed germination /
- antioxidative enzymes

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图 1 NaCl胁迫下异甜菊醇浸种对油菜根系活力的影响

注：不同字母表示不同处理间存在显著差异（P＜0.05），下同。

Figure 1. Effect of pre-soaking seeds in isosteviol solution on root activity of B. napus seedlings under salt stress

Note: Data with different letter indicate significant difference between different treatments at P ＜0.05. Same for the following unless stated otherwise.

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图 2 NaCl胁迫下异甜菊醇浸种对油菜幼苗（鲜样）叶绿素含量的影响

Figure 2. Effect of pre-soaking seeds in isosteviol solution on chlorophyll content of B. napus seedlings (fresh samples) under salt stress

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图 3 NaCl胁迫下异甜菊醇浸种对油菜幼苗子叶（鲜样）丙二醛含量的影响

Figure 3. Effect of pre-soaking seeds in isosteviol solution on MDA content of B. napus seedlings (fresh samples) under salt stress

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图 4 NaCl胁迫下异甜菊醇浸种对油菜幼苗子叶（鲜样）抗氧化酶活性的影响

Figure 4. Effects of pre-soaking seeds in isosteviol solution on SOD, POD, CAT activities of B. napus seedlings (fresh samples) under salt stress

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表 1 NaCl胁迫对油菜种子发芽指标的影响

Table 1. Effect of salt stress on seed germination of B. napus

NaCl 浓度 NaCl concentration/（mmol·L⁻¹）	发芽率 Germination rate/%	发芽指数 Germination index	活力指数 Vitality index	苗高 Stem length/cm	根长 Root length/cm
0（CK₁）	93.00±1.51 Aa	43.71±0.92 Aa	576.97±5.80 Aa	3.40±0.28 Aa	9.80±1.41 Aa
40	91.30±2.62 Aab	41.02±1.41 Aab	500.44±3.30 Bb	3.50±0.32 Aa	8.70±1.12 Bb
80	90.70±1.71 ABb	37.61±1.31 Bb	304.64±4.60 Cc	2.70±0.56 Bb	5.40±0.71 Cc
100	90.20±1.12 ABb	36.82±1.72 Bbc	217.24±2.30 Dd	2.30±0.73 Cbc	3.60±0.42 Dd
120	85.10±0.91 Bc	32.41±1.91 Cc	184.74±1.91 Ee	2.20±1.11 Cc	3.50±0.41 Dd
140	73.60±1.61 Cd	26.53±0.72 Dd	124.69±1.80 Ff	1.80±0.82 Dd	2.90±0.11 Ee
160	49.50±1.72 De	12.26±0.41 Ee	31.88±2.30 Gg	1.20±1.38 Ee	1.40±0.11 Ff
注：同一列无相同大写字母表示处理间差异极显著（P＜0.01）；同一列无相同小写字母表示处理间差异显著（P＜0.05）；CK₁为去离子水浸种和发芽处理组。 Note: Data with different capitalized letters on same column indicate significant difference between treatments at P＜0.01; those with same lowercase letters on same column indicate significant difference between treatments at P＜0.05; CK₁ indicates control group by soaking and germinating seeds in deionized water.

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表 2 NaCl胁迫下异甜菊醇浸种对油菜种子发芽及幼苗生长的影响

Table 2. Effects of pre-soaking in isosteviol solution on seed germination and seedling growth of B. napus under salt stress

异甜菊醇浓度Isosteviol concentration/ （mol·L⁻¹）	发芽率 Germination rate/%	发芽指数 Germination index	活力指数 Vitality index	苗高 Stem length/cm	根长 Root length/cm
0（CK₁）	92.50±1.52 a	42.82±0.90 a	560.94±5.82 a	3.50±0.28 a	9.60±1.40 a
0（CK₂）	74.70±2.61 e	29.13±0.81 d	136.91±1.72 f	1.70±0.31 e	3.00±0.10 f
1×10⁻¹⁰	79.31±1.42 cd	34.13±1.12 c	211.61±0.92 e	2.20±0.51 d	4.00±0.11 e
1×10⁻⁹	85.22±1.20 b	37.04±1.93 b	288.91±1.71 c	2.90±0.20 b	4.90±0.22 c
1×10⁻⁸	84.11±2.10 b	38.75±1.01 b	337.13±2.83 b	2.90±0.41 b	5.80±0.23 b
1×10⁻⁷	81.63±1.61 c	36.91±1.04 bc	265.75±2.74 cd	2.60±0.51 c	4.60±0.41 cd
1×10⁻⁶	77.52±2.02 d	35.92±1.53 c	255.03±1.73 d	2.60±0.42 c	4.50±0.32 d
注：CK₁为去离子水浸种、发苗处理组；CK₂为去离子水浸种、140 mmol·L⁻¹ NaCl胁迫发苗处理组，下同。 Note: CK₁ indicates control group by soaking and germinating seeds in deionized water. CK₂ indicates control group by pre-soaking seeds in deionized water and germinating in medium containing NaCl 140 mmol·L⁻¹. Same for the following unless stated otherwise.

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