Effects of Slow-release Fertilizer on Yield and Quality of Oolong Tea under Reduced Nitrogen Fertilization
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摘要:
目的 研究氮肥减量下缓释肥对乌龙茶鲜叶产量、品质的影响,为茶园减量施肥提供参考。 方法 采用武夷岩茶黄观音为试验材料,以不施氮肥(CK1)和常规施肥(CK2)为对照,设置缓释氮肥减量10%(T1)、20%(T2)、30%(T3)和40%(T4)等4个处理。 结果 与不施氮肥相比,缓释肥显著提高土壤有机质含量、pH值、茶叶产量、SPAD值、含氮量、氨基酸含量。T1、T2与CK2相比,茶叶产量无差异;有机质含量分别提高4.01%和3.10%;pH值分别提高5.82%和2.41%;氨基酸含量分别提高10.11%和9.04%;酚氨比分别降低10.11%和5.57%。对6个处理的成分指标进行主成分分析,提取前2个主成分,累积贡献率达95.20%。经主成分分析,各处理得分差异显著。T1处理t1得分4.418、t2得分1.092,T2处理t1得分2.038和t2得分0.731,两个处理得分较高。 结论 缓释肥氮肥减量10%、20%促进茶鲜叶品质提升,可在茶叶生产上推广应用。 Abstract:Objective Effects of using a slow-release fertilizer to reduce nitrogen (N) demand on the yield and quality of oolong tea were studied for efficient fertilization at plantations. Method Wuyi rock tea, Huangguanyin, bushes were planted under various fertilization treatments including no N fertilizer (CK1), conventional application (CK2), and a slow-release fertilizer with 10% (T1), 20% (T2), 30% (T3) or 40% (T4) N reduction. Yield of fresh leaves per plant and quality of the oolong tea made were determined. Result Compared with CK1, the use of the slow-release fertilizer increased the organic matters and pH in soil as well as the contents of N and amino acids in leaves, yield, and SPAD of the plants. The leaf yields under CK2, T1 and T2 did not differ significantly. But, in comparison to CK2, T1 resulted in 4.01% higher on soil organic matters, 5.82% lower on pH, 10.11% greater on amino acid content in tea leaves, and 10.11% lower on polyphenols/amino acid ratio; while T2 rendered 3.10% higher on soil organic matters, 2.41% lower on pH, 9.04% greater on amino acid content in tea leaves, and 5.57% lower on polyphenols/amino acid ratio. A principal component analysis on these criteria showed the top two principal components contributed 95.20% of the total. The scores on the components differed significantly with those for T1 to be 4.418 on the top first component and 1.092 on the second component; and those for T2, 2.038 on the first component and 0.731 on the second component. Conclusion By using the slow-release fertilizer to replace 10% or 20% N, both yield and quality of oolong tea were improved. -
Key words:
- Oolong tea /
- slow-release fertilizer /
- yield /
- quality /
- principal component analysis
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表 1 田间试验施肥量设计
Table 1. Experimental design for field fertilization
处理
Treatment施肥量 Nitrogen application/(kg·hm−2) 减氮量Nitrogen reduction/% N P2O5 K2O CK1 0 105 140 100 CK2 350 105 140 0 T1 315 105 140 10 T2 280 105 140 20 T3 245 105 140 30 T4 210 105 140 40 表 2 不同处理对土壤有机质含量、pH值的影响
Table 2. Effects of fertilizations on organic matter content and pH of soil
处理
Treatment有机质含量
Organic matter content /
(mg·kg−1)pH值
pH valueCK1 16.56±0.18 cd 5.08±0.08 bc CK2 16.44±0.21 d 4.98±0.09 c T1 17.10±0.28 a 5.27±0.08 a T2 16.95±0.19 ab 5.19±0.09 ab T3 16.84±0.22 abc 5.15±0.10 ab T4 16.76±0.21 bc 5.14±0.11 b 注:结果以均值±标准差表示,同列数据后不同小写字母表示差异显著(P<0.05)。表3、5、6同。
Note: The results were indicated by means±standard deviation. Different lowercase letters of the same row in the table showed significant difference (P<0.05). The same as Table 3, 5, 6.表 3 不同处理对茶鲜叶产量、SPAD值的影响
Table 3. Effects of fertilizations on yield and SPAD of fresh tea leaves
处理
Treatment叶长
Leaf length/cm叶宽
Leaf width/cm叶面积
leaf area/cm2发芽密度
Budding density/(个·m−2)百芽重
100-bud weight/g产量
Yield/(kg·hm−2)SPAD值
SPAD ValueCK1 6.52±0.44 b 2.47±0.29 a 11.31±1.99 b 752.98±33.11 c 91.12±1.75 c 6 723.36±250.44 c 41.55±6.63 c CK2 7.35±0.60 a 2.73±0.27 a 14.13±2.45 ab 830.12±23.05 a 95.84±2.07 a 7 797.06±293.30 a 56.78±5.47 a T1 7.42±0.61 a 2.79±0.26 a 14.56±2.52 a 835.63±29.76 a 95.89±1.63 a 7 852.50±262.19 a 58.21±6.15 a T2 7.36±0.45 a 2.73±0.30 a 14.17±2.63 ab 819.10±28.60 a 95.46±1.41 ab 7 663.14±281.69 a 57.45±6.47 a T3 7.35±0.60 ab 2.66±0.25 a 13.17±2.01 ab 804.41±29.47 ab 95.38±1.76 ab 7 518.24±253.24 a 55.26±4.98 ab T4 7.04±0.47 ab 2.60±0.27 a 12.50±2.18 ab 775.02±29.47 b 93.57±1.02 b 7 107.30±205.63 b 48.73±5.65 bc 表 4 茶鲜叶产量指标相关性分析
Table 4. Correlation among indicators on leaf yield of tea plants
项目
Items叶长
Leaf length叶宽
Leaf width叶面积
leaf area发芽密度
Budding density百芽重
100-bud weight产量
YieldSPAD值
SPAD Value叶长 1 叶宽 0.948** 1 叶面积 0.933** 0.995** 1 发芽密度 0.925** 0.980** 0.986** 1 百芽重 0.994** 0.958** 0.949** 0.954** 1 产量 0.955** 0.983** 0.984** 0.996** 0.977** 1 SPAD值 0.985** 0.974** 0.972** 0.968** 0.991** 0.984** 1 注:*、**分别表示0.05、0.01水平上差异显著。
Note: *,** indicated significant at 0.05 and 0.01 level, respectively.表 5 不同处理对茶鲜叶含氮量、吸氮量和氮素利用率的影响
Table 5. Effects of fertilizations on N content, uptake and utilization efficiency of tea leaves
处理 Treatment 含氮量 N content/% 吸氮量 N uptake/(kg·hm−2) 氮素利用率 NUE/% CK1 3.55±0.15 e 62.72±4.23 d - CK2 4.29±0.18 ab 88.11±5.18 b 7.25±0.38 c T1 4.48±0.16 a 95.66±4.19 a 10.46±0.99 a T2 4.21±0.15 bc 85.62±5.81 b 8.18±1.01 bc T3 4.09±0.15 cd 83.15±4.31 bc 8.34±0.50 b T4 3.99±0.07 d 79.40±3.20 c 7.94±0.72 bc 表 6 不同处理对茶鲜叶品质成分的影响
Table 6. Effect of fertilizations on quality of fresh tea leaves
处理
Treatment水浸出物
Water extracts/%氨基酸
Amino acids/%茶多酚
Tea polyphenols/%咖啡碱
Caffeine/%黄酮类
Flavonoids/(mg·g−1)酚氨比
Tea polyphenols/Free amino acidsCK1 39.72±0.82 c 1.52±0.07 c 19.49±0.53 a 2.23±0.06 c 12.61±0.66 a 12.84±0.70 a CK2 43.28±0.67 a 1.88±0.06 b 18.24±0.74 bc 2.40±0.11 ab 11.93±0.45 a 9.69±0.63 cd T1 43.76±0.51 a 2.07±0.10 a 18.01±0.76 c 2.50±0.10 a 11.80±0.75 a 8.71±0.75 d T2 42.73±0.68 a 2.05±0.05 a 18.80±0.50 abc 2.42±0.07 ab 12.14±0.57 a 9.15±0.22 d T3 41.39±0.70 b 1.81±0.11 b 19.14±0.66 ab 2.38±0.12 abc 12.40±0.42 a 10.53±0.28 bc T4 40.95±0.68 b 1.63±0.05 c 19.35±0.35 a 2.29±0.08 bc 12.56±0.53 a 11.33±0.48 b -
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