印度梨形孢对植物生长调节剂在水稻上过量使用的缓解作用

袁听; 覃金兰; 何珊; 熊翔; 孙健; 杨志强; 何勇; 田志宏

doi:10.19303/j.issn.1008-0384.2020.04.006

印度梨形孢对植物生长调节剂在水稻上过量使用的缓解作用

doi: 10.19303/j.issn.1008-0384.2020.04.006

袁听^1,,
覃金兰¹,
何珊¹,
熊翔¹,
孙健²,
杨志强²,
何勇¹,
田志宏^1, ,

1.
长江大学生命科学学院，湖北　荆州　434025
2.
湖北龙旗生物科技有限公司，湖北　江陵　434108

基金项目: 国家重点研发计划项目（2016YFD0100101-05）；湖北省生物菌肥工程技术研究中心项目（GCZX2012042）；广东省应用植物学重点实验室开放课题（AB2018008）

详细信息

作者简介:
袁听（1996−），男，硕士研究生，研究方向：植物生物技术（E-mail：250210255@qq.com）

通讯作者:
田志宏（1966−），男，博士，教授，博士生导师，研究方向：植物遗传与分子生物学（E-mail：zhtian@yangtzeu.edu.cn）

中图分类号: S 511
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- 被引次数: 0
出版历程
- 收稿日期: 2019-12-24
- 修回日期: 2020-01-23
- 刊出日期: 2020-04-01

Mitigating Ill-effect of Plant Growth Regulator Overuse on Rice Plants by Piriformospora indica

1.
College of Life Science, Yangtze University, Jingzhou, Hubei　434025, China
2.
Hubei Longqi Biotechnology Co., Ltd., Jiangling, Hubei　434108, China

摘要

摘要: 目的植物生长调节剂过量使用会对植物的生长造成不利影响，严重情况下甚至抑制植物生长，而印度梨形孢（Piriformospora indica，简称Pi）是一种植物根部内生菌，能增强植物的抗逆能力，探究印度梨形孢对植物生长调节剂在水稻上过量使用的危害是否具有缓解作用可为缓解植物生长调节剂胁迫提供方法依据。方法利用国际水稻营养液培养水稻，施加过量植物生长调节剂（2,4-D或PP333），水培15 d后，分别测量各组水稻株高、根长、根数、根系活力及叶绿素含量，观察叶片ROS染色情况，分析在印度梨形孢是否接种的情况下，2,4-D或PP333对水稻叶片中抗氧化酶（SOD、POD、CAT）的活性变化影响。结果施加过量植物生长调节剂，水稻生长状况受到抑制，接种过印度梨形孢的水稻受影响较未接种小；+Pi、+2,4-D组与−Pi、+2,4-D组相比，株高增加26.02%，根长增加17.27%，根数增加30.77%，叶绿素含量增加64.71%，根系活力增加43.72%；+Pi、+PP333组与−Pi、+PP333组相比，株高增加36.79%，根系活力增加23.64%；接种印度梨形孢以及2,4-D或PP333处理，使水稻叶片中ROS含量增加；2,4-D或PP333处理使水稻SOD、POD、CAT活性升高，接种过印度梨形孢的水稻，抗氧化酶活性维持在正常水平。结论印度梨形孢对植物生长调节剂在水稻上过量使用具有缓解作用。植物生长调节剂过量使用的情况下，接种印度梨形孢的水稻株高增高、根系发达，植株整体发育比未接种印度梨形孢的水稻良好，印度梨形孢可能通过恢复水稻活性氧系统平衡，一定程度上减少水稻受植物生长调节剂过量使用的抑制作用。
- 印度梨形孢 /
- 水稻 /
- 植物生长调节剂 /
- 抗氧化酶 /
- 活性氧
Abstract: Objective Potential of applying an endophyte in plant roots, Piriformospora indica (Pi), to mitigate the ill-effect of excessive usage of plant growth regulators on rice paddies was investigated. Method Rice seedlings were hydroponically grown in a nutrient solution for 15 d. The height and chlorophyll content of the plants as well as the length, number, and vitality of the roots were monitored. ROS in the leaves was examined by a staining method. Effects of 2,4-D or PP333 treatment with or without Pi addition on the activities of antioxidant enzymes (i.e., SOD, POD, and CAT) in leaves were determined. Result Excessive applications of plant growth regulator can adversely affect or even retard the growth of rice plants. This study revealed that if a rice plant was inoculated with Pi it could become less vulnerable to the stress. Comparing 2,4-D treatment without Pi addition, the seedlings grew 26.02% taller, the roots 17.27% longer, the root count 30.77% more, the chlorophyll content 64.71% higher, and the root vitality 43.72% greater with the Pi addition. In the Pi+PP333 group, the plants were 36.79% taller and 23.64% higher in root vitality than without the presence of Pi. The leaf ROS of the rice seedlings increased with Pi, 2,4-D or PP333 in the nutrient solution. Either 2,4-D or PP333 treatment enhanced the SOD, POD and CAT activities, but the Pi inoculation did not exert any significant differences on them. Conclusion It appeared that P. indica could indeed alleviate the stress imposed on rice plants due to overuse of plant growth regulators. By inoculating Pi in the medium, the rice plants could overcome the interference by 2,4-D or PP333, grow well, and develop a robust root system. Restoration of the antioxidant system in rice by Pi might allow the plant to effectively mitigate the ill caused by over-exposure of plant growth regulators.
- Piriformospora indica /
- rice /
- plant growth regulator /
- antioxidant enzyme /
- reactive oxygen species（ROS）

HTML全文

图 1 印度梨形孢定殖检测

Figure 1. Colonization of P. indica

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图 2 印度梨形孢与2,4-D处理水稻的生长状况

Figure 2. Growth of rice seedlings treated with P. indica and 2,4-D

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图 3 印度梨形孢与PP333处理水稻的生长状况

Figure 3. Growth of rice seedlings treated with Pi and PP333

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图 4 印度梨形孢与2,4-D处理水稻叶片（NBT/DBA）染色观察

Figure 4. Stained leaves（NBT/DBA）from rice plants treated with Pi and 2,4-D

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图 5 印度梨形孢与PP333处理水稻叶片（NBT/DBA）染色观察

Figure 5. Stained leaves（NBT/DBA）from rice plants treated with Pi and PP333

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图 6 印度梨形孢与2,4-D作用下水稻叶片中SOD、POD、CAT活性的影响

注：小写字母表示组间差异达到显著水平。图7同。

Figure 6. Effect on SOD, POD and CAT activities in leaves of rice plants treated with Pi and 2,4-D

Note: Data with lowercase letters indicate significant difference between groups. Same for Fig. 7.

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图 7 印度梨形孢与PP333作用下水稻叶片中SOD、POD、CAT活性的影响

Figure 7. Effect on SOD, POD and CAT activities in leaves of rice plants treated with Pi and PP333

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表 1 水稻种子萌发处理

Table 1. Treatment of rice seeds germination

水稻品种 Rice variety	处理 Treatment	对照 Control
9 311	+P. indica	−P. indica
注:“+”表示添加；“−”表示不添加。表2同。 Note: “+” means to add, “−” means not to add. Same for Table 2.

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表 2 水稻幼苗处理

Table 2. Rice seedling culture

处理编号Treatment number	处理 Treatment
1	+Pi、+2,4-D
2	+Pi、－2,4-D
3	－Pi、+2,4-D
4	－Pi、－2,4-D
a	+Pi、+PP333
b	+Pi、－PP333
c	－Pi、+PP333
d	－Pi、－PP333

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表 3 印度梨形孢与2,4-D作用下的水稻相关生物性状

Table 3. Biological traits related to rice under Pi and 2,4-D

生物性状 Biological traits	+Pi, +2,4-D	+Pi, −2,4-D	−Pi, +2,4-D	−Pi, −2,4-D
株高 Plant height/cm	11.80±0.20 b	13.57±0.21 a	8.73±0.75 c	14.53±1.05 a
主根长 Main root length/cm	6.37±0.31 a	6.83±1.44 a	5.27±1.04 b	6.73±1.12 a
根数 Number of root	13.00±2.65 a	13.33±2.08 a	9.00±1.73 b	13.00±2.65 a
叶绿素含量 Chlorophyll content/（mg·g⁻¹）	0.85±0.06 b	1.33±0.15 a	0.30±0.05 c	1.57±0.23 a
根系活力 Root vitality/（mg·g⁻¹·h⁻¹）	6.64±0.59 bc	15.38±2.30 a	4.62±0.85 c	7.70±0.82 b
注：同行数据后不同小写字母表示差异显著（P＜0.05）。表4同。 Note: Data with lowercase letters on same line indicate significant differences（P＜0.05）. Same for Table 4.

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表 4 印度梨形孢与PP333作用下的水稻相关生物性状

Table 4. Biological traits related to rice plants treated with Pi+PP333

生物性状Biological traits	+Pi, +PP333	+Pi, −PP333	−Pi, +PP333	−Pi, −PP333
株高 Plant height/cm	12.27±0.38 b	21.9±1.25 a	8.97±0.50 c	20.6±1.08 a
主根长 Main root length/cm	4.1±0.82 b	7.7±0.4 a	4.5±0.5 b	5.2±0.75 b
根数 Number of root	7.33±0.58 b	8.33±0.58 b	8±0.63 b	10.67±1.15 a
叶绿素含量 Chlorophyll content/（mg·g⁻¹）	1.01±0.13 b	2.31±0.28 a	1.27±0.26 b	2.06±0.24 a
根系活力 Root vitality/（mg·g⁻¹·h⁻¹）	5.70±0.87 ab	7.99±1.26 a	4.61±1.59 bc	2.97±0.67 c

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