猪粪与菌剂配施对山地红壤温室气体排放的影响

王晓朋; 胡坤; 童晨晓; 朱巧莲; 黄昭昶; 毛艳玲

doi:10.19303/j.issn.1008-0384.2019.11.013

猪粪与菌剂配施对山地红壤温室气体排放的影响

doi: 10.19303/j.issn.1008-0384.2019.11.013

王晓朋^{1, 2,},
胡坤^{1, 2},
童晨晓^{1, 2},
朱巧莲^{1, 2},
黄昭昶^{1, 2},
毛艳玲^{1, 2, 3, ,}

1.
福建农林大学资源与环境学院，福建　福州　350002
2.
土壤生态系统健康与调控福建省高校重点实验室，福建　福州　350002
3.
自然生物资源保育利用福建省高校工程研究中心，福建　福州　350002

基金项目: 校科技创新专项基金（CXZX2017226、CXZX2017116）；福建省科技计划重大专项（2017NZ0001）；福建省科技计划高校产学合作项目（KJb16003A）

详细信息

作者简介:
王晓朋（1993 − ），男，硕士研究生，主要从事土壤碳组分研究（E-mail：846250802@qq.com）

通讯作者:
毛艳玲（1972 − ），女，博士，教授，主要从事土壤碳氮循环研究（E-mail：fafum@126.com）

中图分类号: S 153
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出版历程
- 收稿日期: 2019-10-10
- 修回日期: 2019-11-09
- 刊出日期: 2019-11-01

Effect of Bacterial Agent Added to Fertilizer on Greenhouse Gas Emissions of Mountain Red Soil

WANG Xiao-peng^{1, 2
,},
HU Kun^{1, 2},
TONG Chen-xiao^{1, 2},
ZHU Qiao-lian^{1, 2},
HUANG Zhao-chang^{1, 2},
MAO Yan-ling^{1, 2, 3
, ,}

1.
College of Resource and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
University Key Lab of Soil Ecosystem Health and Regulation in Fujian, Fuzhou, Fujian　350002, China
3.
Engineering Research Institute of Conservation & Utilization of Natural Bioresources for Colleges and Universities in Fujian, Fuzhou, Fujian　350002, China

摘要

摘要: 目的研究猪粪与菌剂配施对山地红壤温室气体排放的影响，为减少温室气体排放提供理论依据。方法通过盆栽试验，探讨对照（CK）、复合肥（F）、猪粪（FM）、低量菌剂与猪粪配施（FMI1）、中量菌剂与猪粪配施（FMI2）和高量菌剂与猪粪配施（FMI3）6 种不同处理下土壤温室气体的排放规律。结果（1）施用菌剂可以显著降低CO₂、CH₄和N₂O的排放通量，且高量菌剂与低中量菌剂有显著差异性；（2）高剂量菌剂与CK相比，CO₂累计排放量降低了84.33%，CH₄累计排放量降低了76.39%，N₂O累计排放量降低了86.44%；（3）施用菌剂可以显著降低综合温室效应（GWP），菌剂施用量越大，对温室效应抑制越明显；（4）施用菌剂可以显著提高土壤养分含量。结论在施用肥料的基础上配施菌剂，可以降低山地红壤CO₂、CH₄和N₂O排放通量与综合温室效应，且菌剂剂量越大，效果越佳。
- 菌剂 /
- 猪粪 /
- 山地红壤 /
- 温室气体排放
Abstract: Objective Effect of addition of a bacterial agent to pig manure or a compound fertilize in mountain red soil on the greenhouse gas emissions was investigated. Method In a pot experimentation on the red soil from hilly regions, a compound fertilizer (F), pig manure (FM), combination of FM and a low level bacterial agent (FMI1), combination of FM and a medium level bacterial agent (FMI2), or combination of FM and a high level bacterial agent (FMI3) was applied to examine the differences on the greenhouse gas emissions. Result The addition of the bacterial agent resulted in (1) a reduction on the emission fluxes of CO₂, CH₄ and N₂O with a significant difference between the high level and the low or medium level of the application; (2) an 84.33% decrease on the cumulative CO₂ emission, 76.39% on CH₄, and 86.44% on N₂O over CK; (3) a significantly reduced comprehensive greenhouse effect (GWP) which was enhanced with increasing addition level; and, (4) a significant improvement on the nutrient content in the soil. Conclusion Applying the bacterial agent along with pig manure or a compound fertilizer significantly reduced the emission fluxes of CO₂, CH₄ and N₂O from the mountain red soil and GWP. The higher the dose of the bacterial agent, the greater beneficial effects would result.
- Bacterial agent /
- pig manure /
- mountain red soil /
- greenhouse gas emission

HTML全文

图 1 猪粪配施菌剂对山地红壤CO₂排放的影响

Figure 1. Effects of bacterial agent added to pig manure on CO₂ emission of mountain red soil

下载: 全尺寸图片幻灯片

图 2 猪粪与菌剂配施对山地红壤CO₂累计排放量的影响

注：不同字母表示处理间差异显著（P＜0.05），下同。

Figure 2. Effect of bacterial agent added to pig manure on cumulative CO₂ emission of mountain red soil

Note: Different letters indicate significant difference between treatments (P<0.05). Same for the following.

下载: 全尺寸图片幻灯片

图 3 猪粪与菌剂配施对山地红壤CH₄排放的影响

Figure 3. Effect of bacterial agent added to pig manure on CH₄ emission of mountain red soil

下载: 全尺寸图片幻灯片

图 4 猪粪与菌剂配施对山地红壤CH₄累计排放量的影响

Figure 4. Effect of bacterial agent added to pig manure on cumulative CH₄ emission of mountain red soil

下载: 全尺寸图片幻灯片

图 5 猪粪与菌剂配施对山地红壤N₂O排放的影响

Figure 5. Effect of bacterial agent added to pig manure on N₂O emission of mountain red soil

下载: 全尺寸图片幻灯片

图 6 猪粪与菌剂配施对土壤N₂O累计排放量的影响

Figure 6. Effect of bacterial agent added to pig manure on cumulative N₂O emission of mountain red soil

下载: 全尺寸图片幻灯片

图 7 猪粪与菌剂配施对土壤微生物量碳的影响

Figure 7. Effect of bacterial agent added to pig manure on microbial carbon biomass of mountain red soil

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表 1 猪粪与菌剂配施对山地红壤综合温室效应的影响

Table 1. Effect of bacterial agent added to pig manure on GWP of mountain red soil

处理 Treatment	CH₄增温潜势 GWP_CH₄	N₂O增温潜势 GWP_N₂O	综合温室效应 GWP
CK	114.08±0.19 a	0.36±0.01 a	114.44±0.20 a
F	87.14±0.16 b	0.22±0.07 b	87.35±0.23 b
FM	79.68±0.06 b	0.27±0.02 b	79.95±0.08 b
FMI1	76.14±0.15 b	0.28±0.09 b	76.42±0.24 b
FMI2	70.39±0.20 b	0.25±0.05 b	70.64±0.25 b
FMI3	35.52±0.13 c	0.05±0.00 c	35.58±0.13 c

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表 2 猪粪与菌剂配施对土壤化学性质的影响

Table 2. Effect of bacterial agent added to pig manure on soil chemistry

处理 Treatment	全氮 Total nitrogen（g·kg⁻¹）	全磷 Total phosphorus（g·kg⁻¹）	全钾 Total potassium（g·kg⁻¹）	碱解氮 Alkaline hydrolysis nitrogen（mg·kg⁻¹）	速效磷 Available potassium（mg·kg⁻¹）	速效钾 Available potassium（mg·kg⁻¹）
CK	0.42±0.02 c	0.31±0.01 c	8.38±0.37 c	24.15±3.64 c	7.31±0.60 d	30.42±2.77 c
F	0.47±0.01 c	0.34±0.00 c	9.76±0.17 b	30.39±7.67 b	12.45±1.10 c	40.42±1.70 b
FM	0.71±0.00 a	0.51±0.03 b	9.78±0.14 b	30.08±4.51 b	33.07±4.23 b	56.81±1.39 a
FMI1	0.59±0.02 b	0.61±0.03 a	10.28±0.34 a	34.88±2.63 a	44.19±1.82 a	60.81±0.00 a
FMI2	0.60±0.00 b	0.65±0.03 a	10.63±0.78 a	34.57±2.47 a	45.28±1.85 a	60.00±1.70 a
FMI3	0.61±0.01 b	0.63±0.00 a	11.00±0.23 a	36.17±3.55 a	44.93±1.87 a	61.62±0.00 a

下载: 导出CSV

参考文献(33)

[1]	ROGELJ J, DEN E M, HÖHNE N, et al. Paris Agreement climate proposals need a boost to keep warming well below 2℃ [J]. Nature, 2016, 534(7609): 631−639. doi: 10.1038/nature18307
[2]	SOLOMON S. IPCC: Climate Change The Physical Science Basis [J]. American Geophysical Union, 2007, 9(1): 123−124.
[3]	IPCC. Working Group I Contribution to the IPCC Fifth Assessment Report(AR5), Climate Change 2013: The Physical Science Basis[C].
[4]	FALKOWSKI P, SCHOLES R J, BOYLE E, et al. The global carbon cycle: atest of our knowledge of earth as a system [J]. Science, 2000, 290(5490): 291−296. doi: 10.1126/science.290.5490.291
[5]	BOWDEN R D, DAVIDSON E, SAVAGE K, et al. Chronic nitrogen additions reduce total soil respiration and microbial respiration in temperate forest soils at the Harvard Forest [J]. Forest Ecology & Management, 2004, 196(1): 43−56.
[6]	张鼎华, 叶章发, 罗水发. 福建山地红壤磷酸离子(H₂PO₄^-)吸附与解吸附的初步研究 [J]. 山地学报, 2001, 19(1):19−24. doi: 10.3969/j.issn.1008-2786.2001.01.004 ZHANG D H, YE Z F, LUO S F. The Preliminary Study on P-Adsorption and P-Desorption in Fujian Mountain Red Soils [J]. Journal of Mountain Research, 2001, 19(1): 19−24.(in Chinese) doi: 10.3969/j.issn.1008-2786.2001.01.004
[7]	栾军伟, 向成华, 骆宗诗, 等. 山地红壤呼吸研究进展 [J]. 应用生态学报, 2006, 17(12):2451−2456. doi: 10.3321/j.issn:1001-9332.2006.12.044 LUAN J W, XIANG C H, LUO Z S, et al. Research advance in forest soil respiration [J]. Chinese Journal of Applied Ecology, 2006, 17(12): 2451−2456.(in Chinese) doi: 10.3321/j.issn:1001-9332.2006.12.044
[8]	陈中云, 闵航, 陈美慈, 等. 不同水稻土甲烷氧化菌和产甲烷菌数量与甲烷排放量之间相关性研究 [J]. 生态学报, 2001, 21(9):1498−1505. doi: 10.3321/j.issn:1000-0933.2001.09.015 CHEN Z Y, MIN H, CHEN M C, et al. Studies on relationships among methane emission and methane-oxidizingand methanogenic bacteria in three types of rice-field soil [J]. Acta Ecologica Sinica, 2001, 21(9): 1498−1505.(in Chinese) doi: 10.3321/j.issn:1000-0933.2001.09.015
[9]	沈菊培, 贺纪正. 微生物介导的碳氮循环过程对全球气候变化的响应 [J]. 生态学报, 2011, 31(11):2957−2967. SHEN J P, HE J Z. Responses of microbes-mediated carbon and nitrogen cycles to global climate change [J]. Acta Ecologica Sinica, 2011, 31(11): 2957−2967.(in Chinese)
[10]	杨颂. 不同微生物菌剂对参后地土壤理化性质及酶活性的影响[D]. 长春: 吉林农业大学, 2015. YANG S. Effects of different microbial agents on physicochemical property and enzyme activities in Panax ginseng continuous cropping ground[D]. Changchun: Jilin Agriculture University, 2015. (in Chinese)
[11]	王明友, 杨秀凤, 郑宪和, 等. 复合微生物菌剂对番茄的光合特性及产量品质的影响 [J]. 中国土壤与肥料, 2004(4):37−39. doi: 10.3969/j.issn.1673-6257.2004.04.012 WANG M Y, YANG X F, ZHENG X H, et al. The effect of tomato photosynthesis characteristic and yield and quality by using biological bacterial manure [J]. Soils and Fertilizers, 2004(4): 37−39.(in Chinese) doi: 10.3969/j.issn.1673-6257.2004.04.012
[12]	DENG Q, ZHOU G, LIU S, et al. Responses of soil CO₂ efflux to precipitation pulses in two subtropical forests in southern China [J]. Environmental Management, 2011, 48(6): 1182−1188. doi: 10.1007/s00267-011-9732-2
[13]	ZHANG W, MO J, YU G, et al. Emissions of nitrous oxide from three tropical forests in Southern China in response to simulated nitrogen deposition [J]. Plant & Soil, 2008, 306(1-2): 221−236.
[14]	王誉瑶, 韦中, 徐阳春, 等. 溶磷菌株组合的溶磷效应及对玉米生长的影响 [J]. 植物营养与肥料学报, 2017, 23(1):262−268. doi: 10.11674/zwyf.16087 WANG Y Y, WEI Z, XU Y C, et al. Dissolving capacity of phosphate dissolving bacteria strains combination and their effects on corn growth [J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(1): 262−268.(in Chinese) doi: 10.11674/zwyf.16087
[15]	栗丽, 李廷亮, 孟会生, 等. 菌剂与肥料配施对矿区复垦土壤养分及微生物学特性的影响 [J]. 应用与环境生物学报, 2016(6):1156−1160. LI L, LI T L, MENG H S, et al. Effects of combined application of microbial agents and fertilizers on soil nutrients and microbial characteristics in reclaimed soil [J]. Chinese Journal of Applied and Environmental Biology, 2016(6): 1156−1160.(in Chinese)
[16]	林欣, 王立立, 杨平, 等. 一株溶磷菌对盐地碱蓬修复盐渍土Cd污染的促进效应 [J]. 农业环境科学学报, 2018, 37(5):883−891. doi: 10.11654/jaes.2018-0087 LIN X, WANG L L, YANG P, et al. The promoting effect of phosphorus-solubilizing bacteria on remediation of cadmium-contaminated saline soil by Suaeda salsa [J]. Journal of Agro-Environment Science, 2018, 37(5): 883−891.(in Chinese) doi: 10.11654/jaes.2018-0087
[17]	张丹丹, 莫柳莹, 陈新, 等. 氮沉降对温带山地红壤甲烷氧化菌的影响 [J]. 生态学报, 2017, 37(24):8254−8263. ZHANG D D, MO L Y, CHEN X, et al. Effects of nitrogen addition on methanotrophs in temperate forest soil [J]. Acta Ecologica Sinica, 2017, 37(24): 8254−8263.(in Chinese)
[18]	周丽, 付智丹, 杜青, 等. 减量施氮对玉米/大豆套作系统中作物氮素吸收及土壤氨氧化与反硝化细菌多样性的影响 [J]. 中国农业科学, 2017, 50(6):1076−1087. doi: 10.3864/j.issn.0578-1752.2017.06.009 ZHOU L, FU Z D, DU Q, et al. Effects of reduced N fertilization on crop N uptake, soil ammonia oxidation and denitrification vacteria diversity in maize/soybean relay strip intercropping system [J]. Scientia Agricultura Sinica, 2017, 50(6): 1076−1087.(in Chinese) doi: 10.3864/j.issn.0578-1752.2017.06.009
[19]	高琳, 王立为, 潘志华, 等. 巨大芽孢杆菌对土壤N₂O释放量及土壤微生物的影响 [J]. 中国农业大学学报, 2016, 21(7):76−84. GAO L, WANG L W, PAN Z H, et al. Effects of carbon source and Bacillus megaterium on soil microbial environment and N₂O, CH₄ emission [J]. Journal of China Agricultural University, 2016, 21(7): 76−84.(in Chinese)
[20]	王国强, 常玉妍, 宋星星, 等. 稻草还田下添加DCD对稻田CH₄、N₂O和CO₂排放的影响 [J]. 农业环境科学学报, 2016, 35(12):2431−2439. doi: 10.11654/jaes.2016-0877 WANG G Q, CHANG Y Y, SONG X X, et al. Effects of DCD addition on CH₄, N₂O and CO₂ emissions from paddy field under rice straw incorporation [J]. Journal of Agro-Environment Science, 2016, 35(12): 2431−2439.(in Chinese) doi: 10.11654/jaes.2016-0877
[21]	陈义, 吴春艳, 水建国, 等. 长期施用有机肥对水稻土CO₂释放与固定的影响 [J]. 中国农业科学, 2005, 38(12):2468−2473. doi: 10.3321/j.issn:0578-1752.2005.12.015 CHEN Y, WU C Y, SHUI J G, et al. Emission and fixation of CO₂ from soil system as influenced by long-term application of organic manure in paddy soils [J]. Scientia Agricultura Sinica, 2005, 38(12): 2468−2473.(in Chinese) doi: 10.3321/j.issn:0578-1752.2005.12.015
[22]	任乐, 马秀枝, 范雪松. 不同经营方式及生境对大兴安岭高纬度林区生长盛季山地红壤CO₂、CH₄、N₂O通量的影响 [J]. 生态环境学报, 2015(3):378−386. REN L, MA X Z, FAN X S. Effect of N₂O, CH₄ and CO₂ fluxes in the thriving season of larix gmelinii forest in cold-temperate zone under different forest management and topographic condition [J]. Ecology and Environmental Sciences, 2015(3): 378−386.(in Chinese)
[23]	高德才, 张蕾, 刘强, 等. 生物黑炭对旱地土壤CO₂、CH₄、N₂O排放及其环境效益的影响 [J]. 生态学报, 2015, 35(11):3615−3624. GAO D C, ZHANG L, LIU Q, et al. Effects of biochar on CO₂, CH₄, N₂O emission and its environmental benefits in dryland soil [J]. Acta EcologicaSinica, 2015, 35(11): 3615−3624.(in Chinese)
[24]	胡敏杰, 仝川, 邹芳芳. 氮输入对土壤甲烷产生、氧化和传输过程的影响及其机制 [J]. 草业学报, 2015, 24(6):204−212. doi: 10.11686/cyxb2014313 HU M J, TONG C, ZOU F F. Effects of nitrogen input on CH₄ production, oxidation and transport in soils, and mechanisms: a review [J]. Acta PrataculturaeSinica, 2015, 24(6): 204−212.(in Chinese) doi: 10.11686/cyxb2014313
[25]	王玉英, 胡春胜. 施氮水平对太行山前平原冬小麦-夏玉米轮作体系土壤温室气体通量的影响 [J]. 中国生态农业学报, 2011, 19(5):1122−1128. WANG Y Y, HU C S. Soil greenhouse gas emission in winter wheat/summer maize rotation ecosystem as affected by nitrogen fertilization in the Piedmont Plain of Mount Taihang, China [J]. Chinese Journal of Eco-Agriculture, 2011, 19(5): 1122−1128.(in Chinese)
[26]	贾仲君, 蔡祖聪. 水稻植株对稻田甲烷排放的影响 [J]. 应用生态学报, 2003, 14(11):2049−2053. doi: 10.3321/j.issn:1001-9332.2003.11.053 JIA Z J, CAI Z C. Effects of rice plants on methane emission from paddy fields [J]. Chinese Journal of Applied Ecology, 2003, 14(11): 2049−2053.(in Chinese) doi: 10.3321/j.issn:1001-9332.2003.11.053
[27]	徐文彬, 刘维屏. 温度对旱田土壤N₂O排放的影响研究 [J]. 土壤学报, 2002, 39(1):1−8. doi: 10.3321/j.issn:0564-3929.2002.01.001 XU W B, LIU W P. Effect of temperature on N₂O emissions from sub-tropical upland soils [J]. Acta PedologicaSinica, 2002, 39(1): 1−8.(in Chinese) doi: 10.3321/j.issn:0564-3929.2002.01.001
[28]	黄耀, 焦燕, 宗良钢, 等. 土壤理化特性对麦田N₂O排放影响的研究 [J]. 环境科学学报, 2002, 22(5):598−603. doi: 10.3321/j.issn:0253-2468.2002.05.011 HUANG Y, JIAO Y, ZONG L G, et al. N₂O emission from wheat cultivated soils as influenced by soil physicochemical properties [J]. Acta Scientiae Circumstantiae, 2002, 22(5): 598−603.(in Chinese) doi: 10.3321/j.issn:0253-2468.2002.05.011
[29]	王斌. 新型氮肥对双季稻田温室气体减排的研究[D]. 北京: 中国农业科学院, 2014. WANG B. Research on GHGs reduction of different innovative nitrogen fertilizer from a double rice field [D]. Beijing: Chinese Academy of Agricultural Sciences, 2014. (in Chinese)
[30]	王璠, 徐圣君, 马双龙, 等. 解淀粉芽孢杆菌菌剂对雪菜生长和土壤氧化亚氮排放的影响 [J]. 中国农学通报, 2015, 31(13):229−235. doi: 10.11924/j.issn.1000-6850.2014-2479 WANG P, XU S J, MA S L, et al. Effectof bacillus amyloliquefaciens biofertilizer on brassica junceavar. multiceps growth and N₂O emission from soil [J]. Chinese Agricultural Science Bulletin, 2015, 31(13): 229−235.(in Chinese) doi: 10.11924/j.issn.1000-6850.2014-2479
[31]	赵彤, 蒋跃利, 闫浩, 等. 黄土丘陵区不同坡向对土壤微生物生物量和可溶性有机碳的影响 [J]. 环境科学, 2013, 34(8):3223−3230. ZHAO T, JIANG Y L, YAN H, et al. Effects of different aspects on soil microbial biomass and dissolved organic carbon of the loess hilly area [J]. Environmental Science, 2013, 34(8): 3223−3230.(in Chinese)
[32]	陈安强, 付斌, 鲁耀, 等. 有机物料输入稻田提高土壤微生物碳氮及可溶性有机碳氮 [J]. 农业工程学报, 2015, 31(21):160−167. doi: 10.11975/j.issn.1002-6819.2015.21.021 CHEN A Q, FU B, LU Y, et al. Exogenous organic materials applied to paddy field improving soil microbial biomass C, N and dissolved organic C, N [J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(21): 160−167.(in Chinese) doi: 10.11975/j.issn.1002-6819.2015.21.021
[33]	汤宏, 沈健林, 张杨珠, 等. 秸秆还田与水分管理对稻田土壤微生物量碳、氮及溶解性有机碳、氮的影响 [J]. 水土保持学报, 2013, 27(1):240−246. TANG H, SHEN J L, ZHANG Y Z, et al. Effect of rice straw incorporation and water management on soil microbial biomass carbon, nitrogen and dissolved organic carbon in a rice paddy field [J]. Journal of Soil and Water Conservation, 2013, 27(1): 240−246.(in Chinese)