猪<i>IGFBP</i>2基因多态性及其对苏姜猪肉质性状的影响

陶勇; 陆建豪; 任善茂

doi:10.19303/j.issn.1008-0384.2019.11.007

猪IGFBP2基因多态性及其对苏姜猪肉质性状的影响

doi: 10.19303/j.issn.1008-0384.2019.11.007

江苏农牧科技职业学院，江苏　泰州　225300

基金项目: 江苏高校“青蓝工程”资助项目[2017(15)]；江苏农牧科技职业学院科研项目（NSF201602）

详细信息

作者简介:
陶勇（1975−），男，博士，副教授，主要从事畜禽遗传资源保护与开发利用研究（E-mail: tyrsm1975tt@126.com）

通讯作者:
任善茂（1976−），女，副教授，主要从事动物营养与饲料科学的教学、科研与服务（E-mail: 825587062@qq.com）

中图分类号: S 828
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出版历程
- 收稿日期: 2019-06-26
- 修回日期: 2019-09-04
- 刊出日期: 2019-11-01

Polymorphism of IGFBP2 Gene Relating to Meat Quality of Sujiang Pigs

Jiangsu Animal Husbandry and Veterinary College, Taizhou, Jiangsu　225300, China

摘要

摘要: 目的探讨IGFBP2基因潜在的遗传变异及其与苏姜猪肉质性状之间的关系。方法采用PCR-RFLP技术检测IGFBP2基因在苏姜猪、姜曲海猪、杜洛克猪中的Msp Ⅰ酶切遗传多态性，采用单因素方差分析法分析该多态位点对苏姜猪肉质性状的影响。结果在3个猪种试验群体，IGFBP2基因第2内含子内均发现了1个Msp Ⅰ酶切多态性，存在A、B等位基因，AA、AB、BB基因型，优势等位基因为B，优势基因型为AB，多态信息含量均呈现中度多态。苏姜猪试验群体IGFBP2基因AA、AB型个体大理石纹显著高于BB型（P＜0.05），AA型个体五分制肉色和色差仪测得的a值均显著高于AB、BB型（P＜0.05）。结论苏姜猪试验群体IGFBP2基因第2内含子内PCR-RFLP-Msp Ⅰ多态性与部分肉质性状间存在一定程度的显著性相关，可以作为与猪肉质性状相关的候选基因加以研究。
- 猪 /
- IGFBP2基因 /
- PCR-RFLP-Msp Ⅰ /
- 肉质性状 /
- 关联分析
Abstract: Objective Genetic variation of the insulin-like growth factor binding protein 2 gene (IGFBP2 gene), relating to the meat quality of Sujiang pigs was studied. Method The polymorphism of IGFBP2 gene was detected by PCR-RFLP in Sujiang pigs, Jiangquhai pigs and Duroc pigs, and the association between IGFBP2 gene polymorphism and meat quality traits in Sujiang pigs was analyzed. Result One common Msp Ⅰ polymorphism in the intron 2 of IGFBP2 gene was found in the 3 pig populations. It consisted of 3 genotypes (AA, AB and BB) and two alleles (A and B) with AB being the dominant genotype and B the prevailing allele. The polymorphisms of the genes from the 3 pig species were moderate on their information content. The meat marbling of Sujiang pigs with the AA and AB genotypes was significantly more than that with the BB genotypes ( P< 0.05). The meat color of the pigs with the AA genotype was significantly more intense than that with the AB and BB genotypes ( P<0.05). Conclusion The PCR-RFLP- Msp I polymorphism in the intron 2 of IGFBP2 gene of Sujiang pigs appeared to significantly correlate with the marbling and color of the pork. Consequently, the gene could potentially be used to study the meat quality improvement through breeding.
- pig /
- IGFBP2 gene /
- PCR-RFLP-Msp Ⅰ /
- meat quality /
- correlation

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图 1 IGFBP2基因PCR扩增产物

Figure 1. PCR-amplified product of IGFBP2 gene

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图 2 IGFBP2基因PCR-RFLP分析结果

Figure 2. Results of PCR-RFLP analysis on IGFBP2 gene

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表 1 IGFBP2基因基因型频率和基因频率

Table 1. Genotype and frequency distribution of IGFBP2 gene

品种 Breed	基因型频率 Genotype frequency			等位基因频率 Allele frequency
品种 Breed	AA	AB	BB	A	B
苏姜猪 Sujiang pig	0.16（76）	0.48（229）	0.36（172）	0.40	0.60
姜曲海猪 Jiangquhai pig	0.10（11）	0.59（64）	0.31（34）	0.39	0.61
杜洛克猪 Duroc pig	0.13（29）	0.61（132）	0.26（57）	0.44	0.56
注：括号内数字为个体数，括号外数字为频率。 Note: Number of individuals outside parentheses, frequency in parentheses.

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表 2 IGFBP2基因多态位点纯合度、杂合度、有效等位基因数和多态信息含量

Table 2. Genetic polymorphism parameters ( Ho, He, Ne and PIC ) of IGFBP2 gene

品种 Breed	纯合度 H₀	杂合度 He	有效等位基因数 Ne	多态信息含量 PIC
苏姜猪 Sujiang pig	0.52	0.48	1.92	0.36
姜曲海猪 Jiangquhai pig	0.52	0.48	1.92	0.41
杜洛克猪 Duroc pig	0.50	0.50	2.00	0.38
注：PIC＜0.25：低度多态；0.25＜PIC＜0.5：中度多态；PIC＞0.5：高度多态。 Note: PIC＜0.25: low polymorphism; 0.25＜PIC＜0.5: moderate polymorphism; PIC＞0.5: high polymorphism.

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表 3 苏姜猪IGFBP2基因不同基因型肉质性状间的差异分析

Table 3. Comparison of meat quality of Sujiang pigs with varied IGFBP2 gene

基因型 Genotype	pH	失水率 Water loss rate /%	大理石纹评分 Marbling score	肉色评分 Meat color score	肉色 Meat color
基因型 Genotype	pH	失水率 Water loss rate /%	大理石纹评分 Marbling score	肉色评分 Meat color score	L	a	b
AA	5.57±0.04	20.94±2.04	3.83±0.45a	3.87±0.23a	43.81±0.64	15.92±0.42a	20.08±0.36
AB	5.53±0.02	23.92±0.96	3.78±0.23a	2.63±0.10b	44.17±0.54	14.01±0.37b	20.39±0.27
BB	5.61±0.05	21.83±1.25	2.34±0.18b	2.73±0.15b	44.78±0.60	13.95±0.38b	20.41±0.32
注：同列数据后不同小写字母表示差异显著（P＜0.05）。 Note: Different lowercase letters in the same column represented significant difference (P＜0.05).

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参考文献(14)

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