短时高温对井上蛀果斑螟成虫存活及繁殖的影响

何超; 沈登荣; 白丽雪; 尹立红; 张睿; 袁盛勇; 田学军

doi:10.19303/j.issn.1008-0384.2019.10.011

短时高温对井上蛀果斑螟成虫存活及繁殖的影响

doi: 10.19303/j.issn.1008-0384.2019.10.011

1.
红河学院生命科学与技术学院/云南省高校农作物优质高效栽培与安全控制重点实验室，云南　蒙自　661100
2.
西南林业大学林学院，云南　昆明　650216

基金项目: 云南省地方高校基础研究联合专项面上项目（2018FH001-035）；云南省教育厅科学研究基金项目（2019J1180）；红河学院博士科研启动专项（14bs15）；红河学院生物学重点建设学科项目（2018ZDXK08）；红河学院大学生创新创业训练计划项目（DCXL181069）

详细信息

作者简介:
何超（1979−），男，博士，讲师，研究方向：农业昆虫及害虫综合治理（E-mail：hechao1022@163.com）

通讯作者:
沈登荣（1983−），男，博士，讲师，研究方向：农业昆虫及害虫综合治理（E-mail：ynshdr@126.com）

中图分类号: S 436.639
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出版历程
- 收稿日期: 2019-09-16
- 修回日期: 2019-10-15
- 刊出日期: 2019-10-01

Effects of Short-term High-temperature Exposure on Survival and Reproduction of Adult Assara inouei Yamanaka

1.
College of Life Science and Technology, Honghe University/Crop Cultivation and Safety Control Key Laboratory of Yunnan Province, Mengzi, Yunnan　661100, China
2.
Forestry College, Southwest Forestry University , Kunming, Yunnan　650216 , China

摘要

摘要: 目的研究短时高温对井上蛀果斑螟Assara inouei Yamanaka成虫存活及繁殖的影响，明确该虫对高温的适应性，为准确预测预报该害虫发生提供依据。方法以25℃恒温为对照，将井上蛀果斑螟羽化12 h内成虫在35、38、41℃高温下，分别处理1、2、4、6 h后移至25℃适温继续饲养，观察短时高温处理后成虫的存活、寿命、产卵及卵孵化情况。结果成虫期短时高温处理的温度和持续时间对井上蛀果斑螟成虫的存活及繁殖有显著影响。随处理温度的升高及处理时间的延长，成虫存活率逐渐降低，寿命和产卵期逐渐缩短，单雌平均产卵量和产卵高峰期产卵量亦逐渐减少，卵孵化率下降，但产卵前期无明显变化。其中，35℃处理1、2 h成虫的存活率、寿命、产卵前期、卵孵化率和对照无显著差异，而产卵期和单雌平均产卵量与对照差异显著。41℃处理4、6 h成虫的存活率、寿命、产卵前期、产卵期、单雌平均产卵量、卵孵化率较对照及35℃相同时间处理差异显著。结论短时高温对井上蛀果斑螟成虫的存活及繁殖有不利影响，且随温度的升高和处理时间的延长，影响程度加剧。
- 井上蛀果斑螟 /
- 短时高温 /
- 存活率 /
- 繁殖
Abstract: Objective Effects of short-term high-temperature exposure on survival and reproduction of adult Assara inouei Yamanaka were studied to determine the thermal adaptation of the insect for population control. Method Within 12 h of emergence, adult A. inouei were exposed to 25℃ (CK), 35℃, 38℃, or 41℃ for 1 h, 2 h, 4 h, or 6 h, then moved to a 25°C chamber for feeding and observation. The survival rate, lifespan, fecundity, and egg hatching rate of the insects were monitored. Result Both temperature and duration of the exposures significantly affected the survival and reproduction of A. inouei. The increased temperature and prolonged exposure caused a gradual decline on the survival rate, lifespan, and oviposition of the insects. The average number of eggs laid per female and that at the peak egg-laying period decreased gradually, and the egg hatching rate decreased as well. But the pre-oviposition period was not significantly altered. Exposure to 35°C for 1 h and 2 h did not cause significant differences on the survival rate, lifespan, pre-oviposition period or egg hatching rate, as compared to CK. But it significantly affected the female oviposition period and average number of eggs laid per individual insect. On the other hand, after being exposed to 41°C for 4 h or 6 h, the insects sustained a significantly lower survival rate, lifespan, pre-oviposition and oviposition periods, number of eggs laid per female, and egg hatching rate than those of CK. Conclusion Exposure to a rising temperature up to 41°C in a duration of 4 h to 6 h could be significantly detrimental to the survival and fecundity of adult A. inouei.
- Assara inouei Yamanaka /
- short-term high-temperature exposure /
- survival rate /
- reproduction

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图 1 成虫期短时高温处理后井上蛀果斑螟的产卵前期和产卵期

注：图中数据为平均值±标准误；同一类型柱上不同小写字母表示差异显著（P＜0.05）。图2和图4同。

Figure 1. Pre-oviposition and oviposition periods of adult A. inouei after high-temperature exposures

Note: Data are means±SE. Data followed by different letters in a same category indicate significant difference at 0.05 level. Same for Fig. 2 and 4.

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图 2 成虫期短时高温处理后井上蛀果斑螟的单雌平均产卵量

Figure 2. Average number of eggs laid per female A. inouei after high-temperature exposures

下载: 全尺寸图片幻灯片

图 3 井上蛀果斑螟成虫于35℃（A）、38℃（B）和41℃（C）下处理不同时间后的单雌日产卵量

Figure 3. Number of eggs laid per day by a female A. inouei after exposure to 35°C (A), 38°C (B) or 41°C (C) for various durations

下载: 全尺寸图片幻灯片

图 4 成虫期短时高温处理后井上蛀果斑螟卵的孵化率

Figure 4. Egg hatching rates of A. inouei after high-temperature exposures

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表 1 成虫期短时高温处理后井上蛀果斑螟成虫的存活率和寿命

Table 1. Survival rate and lifespan of adult A. inouei after high-temperature exposures

温度 Temperature/℃	处理时间 Exposure duration/h	存活率 Survival rate/%		成虫寿命 Longevity of adult/d
温度 Temperature/℃	处理时间 Exposure duration/h	♀	♂	♀	♂
35	1	96.31±2.56 a	90.59±1.78 a	14.67±0.87 a	15.62±1.52 a
	2	95.25±3.43 a	89.23±0.35 a	14.54±0.65 a	15.30±0.70 a
	4	94.67±1.52 a	85.28±2.15 a	13.33±1.53 a	12.64±0.16 b
	6	92.39±3.04 ab	78.59±1.57 b	11.45±1.46 b	11.67±0.55 bc
38	1	93.27±2.54 ab	88.57±2.32 a	11.67±0.79 b	12.06±0.32 b
	2	90.13±1.83 b	86.24±1.35 a	12.15±1.00 b	11.33±0.55 bc
	4	86.52±2.9 bc	80.59±1.54 b	11.08±0.43 b	10.83±0.62 c
	6	85.29±1.45 bc	81.48±1.6 b	8.67±0.58 c	7.52±0.43 d
41	1	87.13±2.02 bc	79.35±2.16 b	6.20±0.26 cd	5.64±0.16 e
	2	83.33±1.37 c	80.17±1.47 b	5.04±0.10 d	5.17±0.58 e
	4	74.25±2.43 d	75.49±1.05 bc	4.63±0.38 d	6.18±0.21 de
	6	70.17±1.84 d	71.58±2.31 c	5.37±0.60 d	4.33±0.38 e
25（CK）		96.23±2.56 a	94.68±2.56 a	14.32±0.74 a	15.39±0.24 a
注：表中数据为平均值±标准误；同列数据后不同小写字母表示差异显著（P＜0.05）。 Note: Data are means ± SE. Data followed by different letters within a column idicate significant difference at 0.05 level.

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