不同种植模式七叶一枝花土壤芽胞杆菌多样性研究

苏海兰; 郑梅霞; 朱育菁; 单寄坪

doi:10.19303/j.issn.1008-0384.2019.08.016

不同种植模式七叶一枝花土壤芽胞杆菌多样性研究

doi: 10.19303/j.issn.1008-0384.2019.08.016

1.
福建省农业科学院农业生物资源研究所, 福建福州 350003
2.
福建承天农林科技发展有限公司, 福建南平 354100

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2019R11020019-9

福建省财政专项——福建省农业科学院创新团队建设项目 STIT2017-2-8

福建省农业科学院科技服务团队项目 kjfw20

详细信息

作者简介:
苏海兰(1980-), 女, 硕士, 农艺师, 研究方向:药用植物资源利用与栽培研究(E-mail:363801575@qq.com)

通讯作者:
朱育菁(1972-), 女, 博士, 研究员, 研究方向:农业生物资源与生物防治的研究(E-mail:zyjingfz@163.com)

中图分类号: Q938
计量
- 文章访问数: 2244
- HTML全文浏览量: 304
- PDF下载量: 26
- 被引次数: 0
出版历程
- 收稿日期: 2019-04-12
- 修回日期: 2019-07-14
- 刊出日期: 2019-08-01

Bacillus spp. in Soils at Paris polyphylla Cultivation Fields

1.
Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
2.
Fujian Chengtian Agriculture and Forestry Technical Development Limited Company, Nanping, Fujian 354100, China

摘要

摘要: 目的分析水稻田、山垅和林下3种种植模式七叶一枝花土壤中芽胞杆菌资源多样性，为七叶一枝花的栽培提供理论基础。方法采用五点采样法，分别采集水稻田、山垅田与林下种植模式七叶一枝花根际土壤与非根际土壤样本6份，采用涂布法分离芽胞杆菌，采用16S rRNA基因同源性将其鉴定。结果一共分离获得了87株芽胞杆菌，13个属、35个种，分别属于芽胞杆菌属Bacillus的16个种、赖氨酸芽胞杆菌属Lysinibacillus、类芽胞杆菌属Paenibacillus和假单胞菌属Pseudomonas的各3个种、Bhargavaea、短杆菌属Brevibacterium、Burkholderia、短芽胞杆菌属Brevibacillus、代夫特菌属Delftia、Paenarthrobacter、节杆菌属Arthrobacter、伯克氏菌属泛菌属Pantoea、假单胞菌属Pseudomonas的各1个种。根据分离频度分析得知，七叶一枝花土壤中的芽胞杆菌优势菌群为图瓦永芽胞杆菌B.toyonensis和假蕈状芽胞杆菌B.pseudomycoides。其中，水稻田种植模式土壤中的芽胞杆菌的数量最多，且Margalef、Shannon-Wiener、Pielou和Simpson多样性指数都最高。七叶一枝花根际土壤中的芽胞杆菌数量均大于非根际土壤。结论水稻田蕴藏着丰富的芽胞杆菌种类和数量，多样性高，在七叶一枝花的种植中具有很好的应用前景。
- 芽胞杆菌 /
- 七叶一枝花 /
- 种植模式 /
- 多样性 /
- 分离频度
Abstract: Objective Distribution and diversity of Bacillus-like bacteria in soil of Paris polyphylla cultivation fields were studied. Method Using the five-points sampling methodology, soil specimens were collected from the rhizosphere and non-rhizosphere on the rice paddy, mountain ridge, and understory fields in Nanping, Fujian where P. polyphylla was cultivated. The bacteria were isolated using smear culture on petri dishes followed by 16S rRNA gene sequencing for identification. Result Of the 87 isolates obtained from 6 soil specimens, 35 belonged to 13 genera of Bacillaceae. They included 16 species of Bacillus, 3 species each of Lysinibacillus, Paenibacillus, and Pseudomonas, and one species each of Bhargavaea, Brevibacterium, Burkholderia, Brevibacillus, Delftia, Paenarthrobacter, Arthrobacter, Pantoea, and Pseudomonas. According to the frequencies of these microbes appeared in the isolation process, the dominant Bacillus spp. in the cultivation soils were B. toyonensis and B. pseudomycoides. Among the different field types, the paddy soil held the greatest quantity of Bacillus spp. as well as the highest Margalef, Shannon-Wiener, Pielou, and Simpson diversity indices. And, the rhizosphere soil was richer in the population than non-rhizosphere soil at all sites. Conclusion Paddy soil was rich and diverse in Bacillus-like bacteria which would benefit the cultivation of P. polyphylla.
- Bacillus spp. /
- Paris polyphylla /
- cultivation fields /
- diversity /
- isolation frequency

HTML全文

图 1 芽胞杆菌的菌落形态

Figure 1. Colony morphology of Bacillus spp.

下载: 全尺寸图片幻灯片

图 2 基于16S rRNA基因序列的南平七叶一枝花不同种植模式土壤中芽胞杆菌系统发育树

注：数字表示各节点自发展支持率数值(>50)。

Figure 2. Phylogenetic tree of Bacillus spp. isolated from soil at P. polyphylla cultivation fields in Nanping based on 16S rRNA gene sequences using neighbor-joining method

Note:Data at branching points indicate bootstrap values are greater than 50%.

下载: 全尺寸图片幻灯片

图 3 不同种植模式七叶一枝花土壤芽胞杆菌的多样性指数

注：Ⅰ为水稻田，Ⅱ为山垅田，Ⅲ为林下。

Figure 3. Diversity indices of Bacilus spp. isolated from soil at paddy field (SDT), mountain ridge (SLT) and understory (LX) growing P. polyphylla

Note:Ⅰ.SDT, Ⅱ.SLT, Ⅲ.LX.

下载: 全尺寸图片幻灯片

表 1 从七叶一枝花不同种植模式土壤中分离得到的细菌的种类

Table 1. Bacillus spp. isolated from soil at P. polyphylla cultivation fields

分离来源 Reource	类群 Group	代表菌株 Strains	最相近菌株 Closest match	相似性 Sequence identify/%
水稻田非根际土(SDF) Irrhizosphere soil in paddy field	芽胞杆菌属 Bacillus	FJAT-49195	高地芽胞杆菌 Bacillus altitudinis	100.00
		FJAT-49204	高地芽胞杆菌 Bacillus altitudinis	100.00
		FJAT-49198	钻特省芽胞杆菌 Bacillus drentensis	99.64
		FJAT-49200	太平洋芽胞杆菌 Bacillus pacificus	100.00
		FJAT-49201	阿氏芽胞杆菌 Bacillus aryabhattai	100.00
		FJAT-49211	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49206	阿氏芽胞杆菌 Bacillus aryabhattai	100.00
		FJAT-49207	扁平芽胞杆菌 Bacillus depressus	99.15
		FJAT-49196	假蕈状芽胞杆菌 Bacillus pseudomycoides	98.87
		FJAT-49208	Bacillus pseudomycoides	100.00
		FJAT-49209	假蕈状芽胞杆菌 Bacillus pseudomycoides	97.95
	赖氨酸芽胞杆菌属 Lysinibacillus	FJAT-49203	Lysinibacillus fusiformis	99.72
		FJAT-49202	Lysinibacillus fusiformis	99.72
		FJAT-49199	Lysinibacillus mangiferihumi	99.93
	短芽胞杆菌 Brevibacillus	FJAT-49197	耐寒短杆菌 Brevibacterium frigoritolerans	99.79
	Paenarthrobacter	FJAT-49205	Paenarthrobacter ilicis	99.57
	类芽胞杆菌属 Paenibacillus	FJAT-49210	Paenibacillus silvae	99.50
水稻田根际土 (SDG)Rhizosphere soil in paddy field	芽胞杆菌属 Bacillus	FJAT-49212	图瓦永芽胞杆菌 Bacillus toyonensis	99.79
		FJAT-49218	图瓦永芽胞杆菌 Bacillus toyonensis	100.00
		FJAT-49223	图瓦永芽胞杆菌 Bacillus toyonensis	100.00
		FJAT-49225	Bacillus pseudomycoides	100.00
		FJAT-49219	假蕈状芽胞杆菌 Bacillus pseudomycoides	99.79
		FJAT-49214	阿氏芽胞杆菌 Bacillus aryabhattai	100.00
		FJAT-49215	高地芽胞杆菌 Bacillus altitudinis	100.00
		FJAT-49216	Bacillus albus	99.65
		FJAT-49213	泰门芽胞杆菌 Bacillus timonensis	98.94
		FJAT-49224	钻特省芽胞杆菌 Bacillus drentensis	99.49
		FJAT-49220	Bacillus mobilis	99.79
		FJAT-49227	Bacillus mobilis	100.00
		FJAT-49228	科研中心芽胞杆菌 Bacillus cecembensis	97.19
		FJAT-49229	扁平芽胞杆菌 Bacillus depressus	98.65
	类芽胞杆菌属 Paenibacillus	FJAT-49222	乐金类芽胞杆菌 Paenibacillus elgii	99.64
		FJAT-49226	蜂房类芽胞杆菌 Paenibacillus alvei	99.09
	赖氨酸芽胞杆菌属 Lysinibacillus	FJAT-49217	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	99.72
		FJAT-49221	纺锤形赖氨酸芽胞杆菌 Lysinibacillus fusiformis	100.00
林下非根际土 (LXF) Irrhizosphere soil in understory	芽胞杆菌属 Bacillus	FJAT-49230	Bacillus proteolyticus	99.93
		FJAT-49238	Bacillus proteolyticus	99.86
		FJAT-49237	图瓦永芽胞杆菌 Bacillus toyonensis	100.00
		FJAT-49240	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49231	Bacillus paramycoides	100.00
	代夫特菌 Delftia	FJAT-49233	湖生代夫特菌 Delftia lacustris	99.86
	赖氨酸芽胞杆菌属 Lysinibacillus	FJAT-49232	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	97.96
		FJAT-49234	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	99.58
		FJAT-49235	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	98.85
	类芽胞杆菌属 Paenibacillus	FJAT-49236	蜂房类芽胞杆菌 Paenibacillus alvei	99.02
	短芽胞杆菌 Brevibacillus	FJAT-49239	侧胞短芽胞杆菌 Brevibacillus laterosporus	99.57
	伯克霍尔德菌 Burkholderia	FJAT-49241	稳定伯克霍尔德菌 Burkholderia stabilis	99.79
林下根际土(LXG) Rhizosphere soil in understory	芽胞杆菌属 Bacillus	FJAT-49242	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49243	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49245	Bacillus paramycoides	100.00
		FJAT-49246	Bacillus paramycoides	100.00
		FJAT-49247	假蕈状芽胞杆菌 Bacillus pseudomycoides	99.86
	赖氨酸芽胞杆菌属 Lysinibacillus	FJAT-49248	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	99.58
		FJAT-49252	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	99.58
		FJAT-49244	解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus	99.58
	假单胞菌 Pseudomonas	FJAT-49251	扁平假单胞菌 Pseudomonas koreensis	99.57
		FJAT-49249	霍氏假单胞菌	99.43
	Bhargavaea	FJAT-49250	Bhargavaea ginsengi	99.72
山垅非根际土(SLF) Irrhizosphere soil in mountain ridge	芽胞杆菌属 Bacillus	FJAT-49253	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49263	图瓦永芽胞杆菌 Bacillus toyonensis	99.93
		FJAT-49264	图瓦永芽胞杆菌 Bacillus toyonensis	100.00
		FJAT-49262	假蕈状芽胞杆菌 Bacillus pseudomycoides	98.87
		FJAT-49254	假蕈状芽胞杆菌 Bacillus pseudomycoides	100.00
		FJAT-49256	赛门枝芽胞杆菌 Bacillus siamensis	99.65
		FJAT-49257	阿氏芽胞杆菌 Bacillus aryabhattai	100.00
		FJAT-49255	Bacillus albus	100.00
		FJAT-49259	维德曼芽胞杆菌 Bacillus wiedmannii	100.00
	假单胞菌 Pseudomonas	FJAT-49258	Pseudomonas granadensis	99.43
		FJAT-49260	Pseudomonas granadensis	99.43
	泛菌属 Pantoea	FJAT-49261	Pantoea rodasii	98.72
山垅非根际土(SLF) Rhizosphere soil in mountain ridge	芽胞杆菌属 Bacillus	FJAT-49265	Bacillus proteolyticus	99.93
		FJAT-49266	Bacillus albus	100.00
		FJAT-49269	图瓦永芽胞杆菌 Bacillus toyonensis	99.86
		FJAT-49270	维德曼芽胞杆菌 Bacillus wiedmannii	100.00
		FJAT-49271	高地芽胞杆菌 Bacillus altitudinis	100.00
		FJAT-49273	阿氏芽胞杆菌 Bacillus aryabhattai	100.00
		FJAT-49277	吉氏芽胞杆菌 Bacillus gibsonii	99.86
		FJAT-49280	扁平芽胞杆菌 Bacillus depressus	99.23
		FJAT-49281	扁平芽胞杆菌 Bacillus depressus	99.93
		FJAT-49267	假蕈状芽胞杆菌 Bacillus pseudomycoides	99.93
	假单胞菌 Pseudomonas	FJAT-49272	基尔假单胞菌 Pseudomonas koreensis	99.64
		FJAT-49268	基尔假单胞菌 Pseudomonas koreensis	99.64
		FJAT-49275	霍氏假单胞菌 Pseudomonas rhodesiae	99.43
	赖氨酸芽胞杆菌属 Lysinibacillus	FJAT-49274	纺锤形赖氨酸芽胞杆菌 Lysinibacillus fusiformis	99.79
	代夫特菌属 Delftia	FJAT-49278	食酸丛毛单胞菌 Delftia acidovorans	99.93
	绿芽胞杆菌 Viridibacillus	FJAT-49279	田地绿芽胞杆菌 Viridibacillus arvi	100.00
	节杆菌 Arthrobacter	FJAT-49276	Arthrobacter bambusae	99.35

下载: 导出CSV

表 2 不同种植模式七叶一枝花土壤芽胞杆菌的分离频度(%)和平均数量(×10⁵ cfu·g^-1)

Table 2. Appearance frequency (%) and average quantity (×10⁵cfu·g^-1) of Bacillus spp. isolated from soil specimens

芽胞杆菌种类 Bacillus-like species	SDG		SDF		SLG		SLF		LXG		LXF		总体 Total
芽胞杆菌种类 Bacillus-like species	分离频度	数量	分离频度	数量	分离频度	数量	分离频度	数量	分离频度	数量	分离频度	数量	分离频度	数量
Bacillus altitudinis	50	18	50	9	50	3	0	0	0	0	0	0	50.00	30
Bacillus pseudomycoides	50	45	50	16	50	4	50	20	50	7	0	0	83.33	92
Brevibacterium frigoritolerans	0	0	50	4	0	0	0	0	0	0	0	0	16.67	4
Bacillus drentensis	50	8	50	3	0	0	0	0	0	0	0	0	33.33	11
Lysinibacillus mangiferihumi	0	0	50	2	0	0	0	0	0	0	0	0	16.67	2
Bacillus pacificus	0	0	50	15	0	0	0	0	0	0	0	0	16.67	15
Bacillus aryabhattai	50	32	50	17	50	10	50	6	0	0	0	0	66.67	65
Lysinibacillus fusiformis	50	1	50	11	50	4	0	0	0	0	0	0	50.00	16
Paenarthrobacter ilicis	0	0	50	1	0	0	0	0	0	0	0	0	16.67	1
Bacillus depressus	50	4	50	4	50	10	0	0	0	0	0	0	50.00	18
Paenibacillus silvae	0	0	50	3	0	0	0	0	0	0	0	0	16.67	3
Bacillus toyonensis	50	24	50	7	50	15	50	7	50	92	50	23	100.00	168
Bacillus timonensis	50	49	0	0	0	0	0	0	0	0	0	0	16.67	49
Bacillus albus	50	22	0	0	50	4	50	11	0	0	0	0	50.00	37
Lysinibacillus xylanilyticus	50	13	0	0	0	0	0	0	50	44	50	17	50.00	74
Bacillus mobilis	50	15	0	0	0	0	0	0	0	0	0	0	16.67	15
Paenibacillus elgii	50	4	0	0	0	0	0	0	0	0	0	0	16.67	4
Paenibacillus alvei	50	1	0	0	0	0	0	0	0	0	50	2	33.33	3
Bacillus cecembensis	50	36	0	0	0	0	0	0	0	0	0	0	16.67	36
Bacillus proteolyticus	0	0	0	0	50	1	0	0	0	0	50	31	33.33	32
Bacillus paramycoides	0	0	0	0	0	0	0	0	50	11	50	3	33.33	14
Delftia lacustris	0	0	0	0	0	0	0	0	0	0	50	1	16.67	1
Brevibacillus laterosporus	0	0	0	0	0	0	0	0	0	0	50	1	16.67	1
Burkholderia stabilis	0	0	0	0	0	0	0	0	0	0	50	11	16.67	11
Pseudomonas rhodesiae	0	0	0	0	50	14	0	0	50	52	0	0	33.33	66
Bhargavaea ginsengi	0	0	0	0	0	0	0	0	50	29	0	0	16.67	29
Pseudomonas koreensis	0	0	0	0	50	10	0	0	50	1	0	0	33.33	11
Bacillus siamensis	0	0	0	0	0	0	50	7	0	0	0	0	16.67	7
Pseudomonas granadensis	0	0	0	0	0	0	50	12	0	0	0	0	16.67	12
Bacillus wiedmannii	0	0	0	0	50	3	50	3	0	0	0	0	33.33	6
Pantoea rodasii	0	0	0	0	0	0	50	8	0	0	0	0	16.67	8
Arthrobacter bambusae	0	0	0	0	50	150	0	0	0	0	0	0	16.67	150
Bacillus gibsonii	0	0	0	0	50	1	0	0	0	0	0	0	16.67	1
Delftia acidovorans	0	0	0	0	50	32	0	0	0	0	0	0	16.67	32
Viridibacillus arvi	0	0	0	0	50	2	0	0	0	0	0	0	16.67	2
总计 Tatal	-	272	-	92	-	263	-	74	-	236	-	89	-	1026
注：分离频度 Separation frequency，数量 Amount。SDF:水稻田七叶一枝花非根际 Irrhizosphere soil of Paris polyphylla in paddy field; SDG:水稻田七叶一枝花根际土 Rhizosphere soil of P. polyphylla in paddy field; LXF:林下七叶一枝花非根际土 Irrhizosphere soil of P. polyphylla in understory; LXG:林下七叶一枝花根际土 Rhizosphere soil of P. polyphylla in understory; SLF:山垅七叶一枝花非根际土 Irrhizosphere soil of P. polyphylla in mountain ridge; SLG:山垅七叶一枝花根际土 Rhizosphere soil of P. polyphylla in mountain ridge.

下载: 导出CSV

参考文献(29)

[1]	陈倩倩, 刘波, 刘国红, 等.华重楼根际土芽胞杆菌多样性研究[J].热带农业科学, 2015, 35(12):103-107. doi: 10.3969/j.issn.1009-2196.2015.12.020 CHEN Q Q, LIU B, LIU G H, et al. Diversity of culturable Bacillus species from Paris Linnaeus rhizosphere soil[J]. Chinese Journal of Tropical Agriculture, 2015, 35(12):103-107.(in Chinese) doi: 10.3969/j.issn.1009-2196.2015.12.020
[2]	梁娟, 郭泽宇, 叶漪.不同土壤水分条件对七叶一枝花光合特性及有效成分皂苷含量的影响[J].植物生理学报, 2014, 50(1):56-60. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwslxtx201401008 LIANG J, GUO Z Y, YE Y.Effects of different soil moisture conditions on photosynthetic characteristics and effective content of saponin of Paris polyphylla[J]. Plant Physiology Communications, 2014, 50(1):56-60.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwslxtx201401008
[3]	张静, 肖国生, 周浓, 等.三峡库区栽培重楼属药用植物根际土壤微生物数量和酶活性的变化[J].中国中医药信息杂志, 2016, 23(10):95-99. http://d.old.wanfangdata.com.cn/Periodical/zgzyyxxzz201610024 ZHANG J, XIAO G S, ZHOU N, et al. Variation of rhizospheric microorganisms and soil enzyme activity of paridis rhizoma cultivated in three gorges reservoir Region[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2014, 50(1):56-60.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgzyyxxzz201610024
[4]	欧洪, 郭冬琴, 林俊杰, 等. AM真菌对滇重楼根际土壤微生物数量及酶活性的影响[J].中药材, 2016, 39(5):948-955. http://d.old.wanfangdata.com.cn/Periodical/zyc201605002 OU H, GUO D Q, LIN J J, et al. Effects of different AM fungi on quantity and enzyme activity of rhizosphere soil microorganism of Paris polyphylla var. yunnanensis[J]. Journal of Chinese Medicinal Materials, 2016, 39(5):948-955.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zyc201605002
[5]	张千和, 周立香, 郭荻.中药材根际和非根际土壤酶和微生物特征[J].西北农业学报, 2014, 23(12):189-196. doi: 10.7606/j.issn.1004-1389.2014.12.029 ZHANG Q H, ZHOU L X, GUO D. Research on soil enzymes and microflora in rhizosphere and non-rhizosphere of traditional chinese medicinal herbs[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2014, 23(12):189-196.(in Chinese) doi: 10.7606/j.issn.1004-1389.2014.12.029
[6]	崔莹, 黄惠琴, 刘敏, 等.八门湾红树林土壤芽胞杆菌分离与多样性分析[J].微生物学通报, 2014, 41(2):229-235. http://d.old.wanfangdata.com.cn/Periodical/wswxtb201402003 CUI Y, HUANG H Q, LIU M, et al. Isolation and diversity analysis of Bacillus-like species from Bamen bay mangrove soil[J]. Microbiology China, 2014, 41(2):229-235.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/wswxtb201402003
[7]	周秋平, 黄惠琴, 崔莹, 等.尖峰岭热带雨林土壤中可培养芽胞杆菌多样性分析[J].广东农业科学, 2015, 42(2):59-63. doi: 10.3969/j.issn.1004-874X.2015.02.012 ZHOU Q P, HUANG H Q, CUI Y, et al. Diversity analysis of culturable Bacillus-like species form Jianfengling tropical rain forest soil[J]. Guangdong Agricultural Sciences, 2015, 42(2):59-63.(in Chinese) doi: 10.3969/j.issn.1004-874X.2015.02.012
[8]	葛慈斌, 郑榕, 刘波, 等.武夷山自然保护区土壤可培养芽胞杆菌的物种多样性及分布[J].生物多样性, 2016, 24(10):1164-1176. doi: 10.17520/biods.2016085 GE C B, ZHENG R, LIU B, et al. Diversity and distribution of cultivable Bacillus-like species in soils col-lected from Wuyishan nature reserve[J]. Biodiversity Science, 2016, 24(10):1164-1176.(in Chinese) doi: 10.17520/biods.2016085
[9]	郑梅霞, 朱育菁, 刘波, 等.云南苍山芽胞杆菌多样性研究[J].福建农业学报, 2019, 34(1):104-116. doi: 10.19303/j.issn.1008-0384.2019.01.015?articleIndex=15 ZHENG M X, ZHU Y J, LIU B, et al. Microbial diversity of Bacillus community in soils at Cangshan Yunnan[J]. Fujian Journal of Agricultural Sciences, 2019, 34(1):104-116.(in Chinese) doi: 10.19303/j.issn.1008-0384.2019.01.015?articleIndex=15
[10]	PISA G, MAGNANI G S, WEBER H, et al. Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil[J]. Brazilian Journal of Medical and Biological Research, 2011, 44(12):1215-1221. doi: 10.1590/S0100-879X2011007500148
[11]	张华勇, 李振高.土壤芽孢杆菌及其资源的持续利用[J].土壤, 2001, 33(2):92-97. http://d.old.wanfangdata.com.cn/Periodical/tr200102009 ZHANG H Y, LI Z G. The sustainable use of resources of soil Bacillus[J]. Soil, 2001, 33(2):92-97.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/tr200102009
[12]	吴海燕, 金荣德, 范作伟, 等.解磷巨大芽孢杆菌(Bacillus megaterium)的溶磷机理探讨[J].吉林农业大学学报, 2014(2):171-175. http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb201402010 WU H Y, JIN R D, FAN Z W, et al. Mechanism of solubilizing phosphate by Bacillus megaterium[J].Journal of Jilin Agricultural University, 2014(2):171-175.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb201402010
[13]	龙苏, 李法峰, 陈明, 等.固氮球形芽孢杆菌与巨大芽孢杆菌的混合增效作用[J].核农学报, 2000, 14(6):337-341. doi: 10.3969/j.issn.1000-8551.2000.06.004 LONG S, LI F F, CHEN M, et al. The enhanced effect of co-culture on nitrogen-fixing activity of B. sphaerium and B. megaterium[J]. Acta Agriculturae Nucleatae Sinica, 2000, 14(6):337-341.(in Chinese) doi: 10.3969/j.issn.1000-8551.2000.06.004
[14]	孙明, 喻子牛.苏云金芽孢杆菌中华亚种CT-43菌株伴胞晶体蛋白的特性[J].微生物学报, 1996(4):303-306. http://www.cnki.com.cn/Article/CJFDTotal-WSXB199604009.htm SUN M, YU Z N. Characterization of insecticidal crystal proteins of Bacillus thuringiensis subsp. Chinensis CT-43[J]. Acta Microbiologica Sinica, 1996(4):303-306.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-WSXB199604009.htm
[15]	张霞, 唐文华, 张力群.枯草芽孢杆菌B931防治植物病害和促进植物生长的作用[J].作物学报, 2007, 33(2):236-241. doi: 10.3321/j.issn:0496-3490.2007.02.010 ZHANG X, TANG W H, ZHANG L Q. Biological control of plant diseases and plant growth promotion by Bacillus subtilis B931[J]. Acta Agronomica Sinica, 2007, 33(2):236-241.(in Chinese) doi: 10.3321/j.issn:0496-3490.2007.02.010
[16]	张建萍, 董乃源, 余浩滨, 等.应用16S rDNA-RFLP方法分析宁夏地区稻田土壤细菌的多样性[J].生物多样性, 2008, 16(6):586-592. doi: 10.3321/j.issn:1005-0094.2008.06.009 ZHANG J P, DONG N Y, YU H B, et al. Bacteria diversity in paddy field soil by 16S rDNA-RFLP analysis in Ningxia[J]. Biodiversity Science, 2008, 16(6):586-592.(in Chinese) doi: 10.3321/j.issn:1005-0094.2008.06.009
[17]	陶金.鄱阳湖湿地围垦后土壤团聚体结构、有机碳及微生物多样性变化的研究[D].南昌: 南昌大学, 2012. http://cdmd.cnki.com.cn/Article/CDMD-11902-1012428898.htm TAO J. The study of changes of SOC content, aggregate structure and evolution of aoil microbial diversity of Poyang Lake[D]. Nanchang: Nanchang University, 2012.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-11902-1012428898.htm
[18]	艾超.长期施肥下根际碳氮转化与微生物多样性研究[D].北京: 中国农业科学院, 2015. http://cdmd.cnki.com.cn/Article/CDMD-82101-1015378889.htm AI C. Carbon and nitrogen transformations and microbial diversity in the rhizosphere soil under long-tern fertilization practices[D]. BeiJing: Chinese Academy of Agricultural Sciences, 2015.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-82101-1015378889.htm
[19]	王丹, 李恋卿, 刘永卓, 等.不同施肥处理对太湖地区水稻土团聚体粒组细菌和真菌组成和多样性的影响[J].土壤, 2012, 44(2):290-296. doi: 10.3969/j.issn.0253-9829.2012.02.018 WANG D, LI L Q, LIU Y Z, et al. Influences of Long-term Fertilization on Bacteria and Fungi Community Structures in Different Aggregate-size Aggregates of Paddy Soil in Taihu Lake Region of China[J]. Soils, 2012, 44(2):290-296.(in Chinese) doi: 10.3969/j.issn.0253-9829.2012.02.018
[20]	丁新景, 敬如岩, 黄雅丽, 等.黄河三角洲刺槐根际与非根际细菌结构及多样性[J].土壤学报, 2017, 54(5):1293-1302. http://d.old.wanfangdata.com.cn/Periodical/trxb201705023 DING X J, JING R Y, HUANG Y L, et al. Bacterial structure and diversity of rhizosphere and bulk soil of Robinia pseudoacacia forests in Yellow River Delta[J]. Acta Pedologica Sinica, 2017, 54(5):1293-1302.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/trxb201705023
[21]	安韶山, 李国辉, 陈利顶.宁南山区典型植物根际与非根际土壤微生物功能多样性[J].生态学报, 2010, 31(18):5225-5234. http://d.old.wanfangdata.com.cn/Periodical/stxb201118014 AN S S, LI G H, CHEN L D. Soil microbial functional diversity between rhizosphere and non-rhizosphere of typical plants in the hilly area of southern Nixia[J]. Acta Ecologica Sinica, 2010, 31(18):5225-5234.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/stxb201118014
[22]	李岩, 何学敏, 杨晓东, 等.不同生境黑果枸杞根际与非根际土壤微生物群落多样性[J].生态学报, 2018, 38(17):5-17. http://d.old.wanfangdata.com.cn/Periodical/stxb201817001 LI Y, HE X M, YANG X D, et al. The microbial community diversity of the rhizosphere and bulk soils of Lycium ruthenicum in different habitats[J]. Acta Ecologica Sinica, 2018, 38(17):5-17.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/stxb201817001
[23]	ROOS T B, de MORAES C M, STURBELLE R T, et al. Probiotics Bacillus toyonensis and Saccharomyces boulardii improve the vaccine immune response to Bovine herpesvirus type 5 in sheep[J]. Research in Veterinary Science, 2017:260-265. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=c44217046d312dac733644cd26518c79
[24]	KANTAS D, PAPATSIROS V G, TASSIS P D, et al. A feed additive containing Bacillus toyonensis Toyocerin(^Ⓡprotects against enteric pathogens in postweaning piglets[J]. Journal of Applied Microbiology, 2015, 118(3):727-738. doi: 10.1111/jam.12729
[25]	郭晓军, 袁洪水, 刘慧娟, 等.假蕈状芽孢杆菌纤溶酶基因的克隆与表达[J].中国医药工业杂志, 2011, 42(1):14-24. doi: 10.3969/j.issn.1001-8255.2011.01.006 GUO X J, YUAN H S, LIU H J, et al. Cloning and expression of fibrinolytic enzyme gene from Bacillus pseudomycoides[J]. Chinese Journal of Pharmaceuticals, 2011, 42(1):14-24.(in Chinese) doi: 10.3969/j.issn.1001-8255.2011.01.006
[26]	KANG H C, KIM N H, JEONG Y J, et al. Biochemical characteristics of a bacteria (Bacillus pseudomycoides) alanine racemase expressed in Escherichia coli[J]. Journal of Applied Biological Chemistry, 2010, 53(3):132-138. doi: 10.3839/jabc.2010.025
[27]	WANG K, YAN P S, CAO L X. Plackett-burman design for media nutrients of biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin[J]. Applied Mechanics & Materials, 2014, 522-524:295-298. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.4028/www.scientific.net/AMM.522-524.295
[28]	喻江.玉米和大豆根内生细菌多样性及促生细菌鉴定评价[D].哈尔滨: 东北农业大学, 2016. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y3189209 YU J. Diversity of root endophytic bacteria and identification of promoting growth bacteria in corn and soybean[D]. Harbing: Northeast Agricultural University, 2016.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y3189209
[29]	陶爱丽.利用小麦内生细菌对小麦秆黑粉病的生物防治研究[D].南宁: 广西大学, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10593-1015436235.htm TAO A L. The biological control research of using endophytic bacteria strain of wheat to control the wheat stem smut[D]. Nanning: Guangxi University, 2014.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10593-1015436235.htm