• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

不同种植模式七叶一枝花土壤芽胞杆菌多样性研究

Bacillus spp. in Soils at Paris polyphylla Cultivation Fields

  • 摘要:
      目的  分析水稻田、山垅和林下3种种植模式七叶一枝花土壤中芽胞杆菌资源多样性,为七叶一枝花的栽培提供理论基础。
      方法  采用五点采样法,分别采集水稻田、山垅田与林下种植模式七叶一枝花根际土壤与非根际土壤样本6份,采用涂布法分离芽胞杆菌,采用16S rRNA基因同源性将其鉴定。
      结果  一共分离获得了87株芽胞杆菌,13个属、35个种,分别属于芽胞杆菌属Bacillus的16个种、赖氨酸芽胞杆菌属Lysinibacillus、类芽胞杆菌属Paenibacillus和假单胞菌属Pseudomonas的各3个种、Bhargavaea、短杆菌属BrevibacteriumBurkholderia、短芽胞杆菌属Brevibacillus、代夫特菌属DelftiaPaenarthrobacter、节杆菌属Arthrobacter、伯克氏菌属泛菌属Pantoea、假单胞菌属Pseudomonas的各1个种。根据分离频度分析得知,七叶一枝花土壤中的芽胞杆菌优势菌群为图瓦永芽胞杆菌B.toyonensis和假蕈状芽胞杆菌B.pseudomycoides。其中,水稻田种植模式土壤中的芽胞杆菌的数量最多,且Margalef、Shannon-Wiener、Pielou和Simpson多样性指数都最高。七叶一枝花根际土壤中的芽胞杆菌数量均大于非根际土壤。
      结论  水稻田蕴藏着丰富的芽胞杆菌种类和数量,多样性高,在七叶一枝花的种植中具有很好的应用前景。

     

    Abstract:
      Objective  Distribution and diversity of Bacillus-like bacteria in soil of Paris polyphylla cultivation fields were studied.
      Method  Using the five-points sampling methodology, soil specimens were collected from the rhizosphere and non-rhizosphere on the rice paddy, mountain ridge, and understory fields in Nanping, Fujian where P. polyphylla was cultivated. The bacteria were isolated using smear culture on petri dishes followed by 16S rRNA gene sequencing for identification.
      Result  Of the 87 isolates obtained from 6 soil specimens, 35 belonged to 13 genera of Bacillaceae. They included 16 species of Bacillus, 3 species each of Lysinibacillus, Paenibacillus, and Pseudomonas, and one species each of Bhargavaea, Brevibacterium, Burkholderia, Brevibacillus, Delftia, Paenarthrobacter, Arthrobacter, Pantoea, and Pseudomonas. According to the frequencies of these microbes appeared in the isolation process, the dominant Bacillus spp. in the cultivation soils were B. toyonensis and B. pseudomycoides. Among the different field types, the paddy soil held the greatest quantity of Bacillus spp. as well as the highest Margalef, Shannon-Wiener, Pielou, and Simpson diversity indices. And, the rhizosphere soil was richer in the population than non-rhizosphere soil at all sites.
      Conclusion  Paddy soil was rich and diverse in Bacillus-like bacteria which would benefit the cultivation of P. polyphylla.

     

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