建阳桔柚两种真菌病原菌的鉴定

葛慈斌; 黄素芳; 阮传清; 肖荣凤; 刘韬

doi:10.19303/j.issn.1008-0384.2019.08.014

建阳桔柚两种真菌病原菌的鉴定

doi: 10.19303/j.issn.1008-0384.2019.08.014

1.
福建省农业科学院农业生物资源研究所, 福建福州 350003
2.
福建省农业科学院生物技术研究所, 福建福州 350003

基金项目:

福建省农业科技跨越计划项目 2005-14

福建省财政专项——福建省农业科学院科技创新团队建设项目 STIT2017-1-11

详细信息

作者简介:
葛慈斌(1973-), 男, 硕士, 副研究员, 研究方向:农业微生物(E-mail:benci2000@sohu.com)

通讯作者:
刘韬(1962-), 男, 教授级高级农艺师, 研究方向:果树学、生态农业(E-mail:liutao1202@sina.com)

中图分类号: S687
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- 被引次数: 0
出版历程
- 收稿日期: 2019-07-15
- 修回日期: 2019-08-24
- 刊出日期: 2019-08-01

Pathogens of Diseases on Jianyang Tangelos

1.
Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
2.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 目的明确引起福建建阳桔柚落叶、枝梢枯死的两种病害的病原菌种类，为防治病害提供基础。方法从建阳桔柚上采集典型发病叶片，进行病原菌的分离、形态观察和菌株rDNA-ITS区序列比对、致病性测定。结果桔柚病叶片中分离出两种主要病原真菌，代表菌株分别为FJAT-32214和FJAT-32151。菌株FJAT-32214在PSA培养基上的菌落颜色为灰褐色，产褐色分生孢子，多呈链状分隔，其rDNA-ITS区序列与互隔交链孢霉Alternaria alternata的ITS序列相似性达99%；菌株FJAT-32151在PSA培养基平板上的菌丝呈灰白色，绒状、茂密，后期会在菌落表面产生砖红色孢子堆，分生孢子柱形或棒状，单胞，无色，其rDNA-ITS区序列与胶孢炭疽菌Colletotrichum gloeosporioides的ITS序列相似性也达99%，通过回接可引起样本致病，分离获得与接种物一致的病原物，符合Koch氏法则。结论通过病原形态学与ITS序列分析，鉴定了发生在建阳桔柚叶片上的两种主要真菌性病原，分别为引起褐斑病的互隔交链孢霉和炭疽病的胶孢炭疽菌。
- 建阳桔柚 /
- 病害鉴定 /
- 互隔交链孢霉 /
- 胶孢炭疽菌
Abstract: Objective To identify the pathogens of the severe defoliation and dieback diseases on Jianyang tangelos. Method Leaf specimens were collected from the diseased tangelo plants in Jianyang city. Microbial strains were isolated for morphological observation, pathogenicity determination, and sequence analysis on internal transcribed spacer (ITS) of ribosomal DNA for taxonomic identification. Result Separately, two strains were considered the pathogens for the diseases. The FJAT-32214 strain formed grayish brown colonies on a PSA medium and produced brown septate spores. Its rDNA-ITS sequences showed a 99% similarity with that of Alternaria alternata from NCBI. The other strain, FJAT-32151, formed on PSA gray-white, velvety, dense colonies with brick-red spore piles on the surface in late stage and produced columnar or rod-like, unicellular, colorless spores. The rDNA-ITS sequence of FJAT-32151 was 99% homologous with that of Colletotrichum gloeosporioides. Each of these isolated strains met Koch requirement with a challenge test by inoculation on healthy plants to show the typical morphological characteristics of the respective fungal disease. Conclusion It was confirmed that A. alternata was the pathogen that caused the brown spots disease, and C. gloeosporioides the anthracnose disease on the tangelos.
- Jianyang tangelo /
- disease diagnosis /
- Alternaria alternata /
- Colletotrichum gloeosporioides

HTML全文

图 1 褐斑病叶片症状

Figure 1. Symptoms of on a tangelo leaf

下载: 全尺寸图片幻灯片

图 2 炭疽病叶片症状

注：A为病叶整体; B为病叶的局部。

Figure 2. Symptoms of anthracnose disease on a tangelo leaf

Note:A: the whole sick leaves; B:part of sick leaf.

下载: 全尺寸图片幻灯片

图 3 菌株FJAT-32214的菌落形态

注：A为正面，B为背面。图 5同。

Figure 3. Morphology of FJAT-32214 colonies

Note:A:faade side, B:reverse side.The same as Fig. 5.

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图 4 菌株FJAT-32214的分生孢子器及分生孢子

Figure 4. Pycnidium and spores of FJAT-32214

下载: 全尺寸图片幻灯片

图 5 菌株FJAT-32151的菌落形态

Figure 5. Morphology of FJAT-32151 colonies

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图 6 菌株FJAT-32151的分生孢子及分生孢子器

注：A为分生孢子，B为分生孢子器。

Figure 6. Spores and pycnidium of strain FJAT-32151

Note:A:spores, B:pycnidium.

下载: 全尺寸图片幻灯片

图 7 菌株FJAT-32214基于ITS序列构建的分子系统发育树

Figure 7. Phylogenetic dendrogram of strain FJAT-32214 and other 11 Alternaria sp. strains based on ITS sequences

下载: 全尺寸图片幻灯片

图 8 菌株FJAT-32151基于ITS序列构建的分子系统发育树

Figure 8. Phylogenetic dendrogram of strain FJAT-32151 and other 11 Colletotrichum sp. strains based on ITS sequences

下载: 全尺寸图片幻灯片

图 9 回接后发病的褐斑病症状

Figure 9. Symptoms of brown spot on leaf after pathogen inoculation

下载: 全尺寸图片幻灯片

图 10 回接后发病的炭疽病症状

Figure 10. Symptoms of anthracnose on leaf after pathogen inoculation

下载: 全尺寸图片幻灯片

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