柑橘果实响应高温、强光胁迫的活性氧代谢研究

万继锋; 李娟; 杨为海; 曾辉; 张汉周; 陈杰忠

doi:10.19303/j.issn.1008-0384.2019.08.008

柑橘果实响应高温、强光胁迫的活性氧代谢研究

doi: 10.19303/j.issn.1008-0384.2019.08.008

万继锋^{1, 2,},
李娟³,
杨为海¹,
曾辉¹,
张汉周¹,
陈杰忠^2, ,

1.
中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室, 广东湛江 524091
2.
华南农业大学园艺学院, 广东广州 510642
3.
仲恺农业工程学院园艺园林学院, 广东广州 510225

基金项目:

国家自然科学基金项目 31372008

广东省科技计划项目 2014A020209081

广东省科技计划项目 2014A020208085

广东省科技计划项目 2015A020209110

详细信息

作者简介:
万继锋(1983—), 男, 硕士, 助理研究员, 研究方向:果树种质资源与栽培育种(E-mail:wanjifeng2002@163.com)

通讯作者:
陈杰忠(1958—), 男, 教授, 研究方向:南方果树的栽培生理研究与教学(E-mail:cjzlxb@scau.edu.cn)

中图分类号: S666
计量
- 文章访问数: 1039
- HTML全文浏览量: 295
- PDF下载量: 26
- 被引次数: 0
出版历程
- 收稿日期: 2019-06-24
- 修回日期: 2019-07-15
- 刊出日期: 2019-08-01

ROS Metabolism of Citrus Fruits in Response to High-temperature-intense-light Stress

1.
Key Laboratory of Ministry of Agriculture and Rural Affairs for Tropical Fruit's Biology, South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China
2.
College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China
3.
College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China

摘要

摘要: 目的研究柑橘果实响应高温、强光胁迫的活性氧代谢，为预防柑橘果实发生日灼以及果品优质生产提供依据。方法在环境调控生长室中模拟高温、强光诱导柑橘果实发生灼伤，并从果实灼伤病程的不同阶段入手，分析了果实灼伤病程中果皮活性氧代谢的变化规律。结果高温、强光胁迫使果皮中O₂^·-大量积累，LOX活性增强，脂质过氧化产物MDA含量显著上升。灼伤初期果皮呈现深黄色小斑点，SOD、POD和PPO活性均显著上升；随着灼伤病情的加剧，果皮变褐，POD活性仍增强，SOD活性显著下降且显著低于正常果，而PPO活性显著下降，但仍显著高于正常果；AsA和GSH含量在整个灼伤病程中均逐步下降。结论在高温、强光胁迫初期果皮呈现深黄色小斑点，柑橘果实果皮组织维持较高的活性氧清除能力，减缓了高温、强光对细胞膜的损伤。果皮呈现深黄色小斑点是预防柑橘果实果皮褐变的重要时间节点。
- 柑橘果实 /
- 高温胁迫强光胁迫 /
- 活性氧代谢
Abstract: Objective The reactive oxygen species (ROS) metabolism of citrus fruits under high temperature and intense light was studied in search for means to mitigate sunburn damage on the fruits. Method Sweet oranges, An-liu (Citrus sinensis Osbeck), grown in pots in an environmentally controlled chamber were exposed to artificial high-temperature-intense-light to create simulated burns on the fruits. Change on the ROS metabolism of the fruits as burn symptoms developed in normality and stages with appearance of dark yellow spots, slight browning, and seriously burnt coloration on the peel were monitored. Result Under the imposed stress, the O₂^·- and MDA contents and LOX activity in pericarp significantly increased. The activities of SOD, POD, and PPO were significantly raised with the appearance of dark yellow spots on the peels. As the browning intensified, SOD significantly fell below the normal level, POD continued to increase, and PPO significantly decreased but remained to be higher than normal. Under the stress, AsA and GSH in the fruits decreased continuously. Conclusion In the early stages, the high-temperature-intense-light stress began generating dark yellow spots on the orange pericarp. However, the fruit was able to sufficiently remove ROS diminishing or preventing damage on the cell membrane. Hence, it was the crucial time for implementation of measures to avoid sunburns on the fruits.
- citrus fruit /
- high-temperature-intense-light stress /
- reactive oxygen species

HTML全文

图 1 诱导柑橘果实灼伤病程中果皮细胞的膜脂过氧化

注：Ⅰ为正常果，Ⅱ为斑点果，Ⅲ为轻度褐变果，Ⅳ为重度褐变果。图中不同小写字母表示差异显著(P < 0.05)。图 2~3同。

Figure 1. Changes in cell membrane lipid peroxidation of Citrus Fruits under high-temperature-intense-light stress

Note:Ⅰ.normal fruit, Ⅱ.dark yellow spots fruit, Ⅲ.slight browning fruit, Ⅳ.serious browning fruit.Different lowercase letters in the figure indicated significant differences (P < 0.05).The same as Fig. 2-3.

下载: 全尺寸图片幻灯片

图 2 诱导柑橘果实灼伤病程中果实抗氧化酶变化

Figure 2. Changes in antioxidant enzymes of Citrus Fruits under high-temperature-intense-light stress

下载: 全尺寸图片幻灯片

图 3 诱导柑橘果实灼伤病程中果皮抗氧化剂的变化

Figure 3. Changes in antioxidants of Citrus Fruits under high-temperature-intense-light stress

下载: 全尺寸图片幻灯片

参考文献(21)

[1]	张格成, 郑重禄.柑橘日烧病的发生与十大综合治理措施[J].四川农业科技, 1996, 1(6):38. http://www.cnki.com.cn/Article/CJFDTotal-SNYK606.038.htm ZHANG G C, ZHENG C L. Occurrence of citrus sunburn and ten comprehensive treatment measures[J]. Sichuan Agricultural Science and Technology, 1996, 1(6):38.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-SNYK606.038.htm
[2]	唐明丽.柑橘日灼果的防治[J].广西园艺, 2003, (4):8. http://d.old.wanfangdata.com.cn/Periodical/gxyy200304028 TANG M L. The prevention and control of the sunburn fruit in citrus[J]. Guangxi Horticulture, 2003(4):8.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gxyy200304028
[3]	万继锋, 李娟, 杨为海, 等.外源调节物质对柑橘果实高温胁迫的抗氧化效应[J].热带作物学报, 2018, 39(8):1548-1552. doi: 10.3969/j.issn.1000-2561.2018.08.012 WAN J F, LI J, YANG W H, et al. The Effect of Exogenous Regulators on Antioxidant Capacity of Citrus Fruits under the Stress of High Temperature[J]. Chinese Journal of Tropical Crops, 2018, 39(8):1548-1552.(in Chinese) doi: 10.3969/j.issn.1000-2561.2018.08.012
[4]	郝燕燕, 李文来, 常月梅.果实日烧发生机理及防止措施[J].山西农业大学学报, 2006, 26(3):296-299. doi: 10.3969/j.issn.1671-8151.2006.03.028 HAO Y Y, LI W L, CHANG Y M. Summary on the mechanism of fruit sunburn development and protective methods[J]. Journal of Shanxi Agricultural University, 2006, 26 (3):296-299.(in Chinese) doi: 10.3969/j.issn.1671-8151.2006.03.028
[5]	KETCHIE D O, BALLARD A L. Environments which cause heat injury to"Valencia"oranges[J]. Proceedings of American Society for Horticultural Science, 1968, 93:166-172. http://cn.bing.com/academic/profile?id=ae0ce692f5372649ce20c72940d71dea&encoded=0&v=paper_preview&mkt=zh-cn
[6]	万继锋, 李娟, 陈杰忠.柑橘日灼病果皮抗氧化代谢的变化[J].园艺学报, 2012, 39(10):2009-2014. http://d.old.wanfangdata.com.cn/Periodical/yyxb201210017 WAN J F, LI J, CHEN J Z. Changes in Antioxidant Metabolism in the Fruit Pericarps of Citrus During Sunburn Development[J]. Acta Horticulturae Sinica, 2012, 39(10):2009-2014.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201210017
[7]	MAO L C, WANG G Z, ZHU C G, et al. Involvement of phospholipase D and lipoxygenase in response to the chilling stress in postharvest cucumber fruits[J]. Plant Science, 2007, 172 (2):400-405. doi: 10.1016/j.plantsci.2006.10.002
[8]	李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社, 2000. LI H S. Principles and Techniques of Plant Physiological and Biochemical Experiment[M]. Beijing:Higher Education Press, 2000.(in Chinese)
[9]	张建光, 陈少春, 李英丽, 等.高温强光胁迫对苹果果皮PPO活性的影响[J].生态学报, 2008, 28 (10):4645-4651. doi: 10.3321/j.issn:1000-0933.2008.10.004 ZHANG J G, CHEN S C, LI Y L, et al. Effect of high temperature and excessive light stresses on PPO activity in apple peel[J]. Acta Ecologica Sinica, 2008, 28 (10):4645-4651.(in Chinese) doi: 10.3321/j.issn:1000-0933.2008.10.004
[10]	FINKEL T, HOLBROOK N J. Oxidant, oxidative stress and biology of ageing[J]. Nature, 2000, 408(6809):239-247. doi: 10.1038/35041687
[11]	LEE S H, AHN S J, IM Y J, et al. Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in fig leaf gourd and cucumber roots[J]. Biochemical and Biophysical Research Communications, 2005, 330(4):1194-1198. doi: 10.1016/j.bbrc.2005.03.098
[12]	HAMEED A, GOHER M, IQBAL N. Drought induced programmed cell death and associated changes in antioxidants, proteases and lipid peroxidation in wheat leaves[J]. Biologia Plantarum, 2013, 57(2):370-374. doi: 10.1007/s10535-012-0286-9
[13]	张玉, 陈昆松, 张上隆.乙酰水杨酸处理对猕猴桃果实成熟衰老的影响及其作用机理[J].植物生理与分子生物学学报, 2002, 28(6):425-432. http://d.old.wanfangdata.com.cn/Periodical/zwslxb200206003 ZHANG Y, CHEN K S, ZHANG S L. Effects of aspirin treatment on Kiwifruit ripening and senescence and its mechanism[J]. Journal of Plant Physiology and Molecular Biology, 2002, 28(6):425-432.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwslxb200206003
[14]	郝燕燕, 黄卫东.苹果日烧病程中果皮抗氧化系统在细胞超微结构的变化[J].植物生理与分子生物学学报, 2004, 30(1):19-26. HAO Y Y, HUANG W D. Changes in Antioxidative System and Cell Ultrastructure in the Fruit Peels of Apple During Sunburn Development[J]. Journal of Plant Physiology and Molecular Biology, 2004, 30 (1):19-26.(in Chinese)
[15]	李秀, 巩彪, 徐坤.外源NO对高温胁迫下姜叶片活性氧代谢的影响[J].园艺学报, 2014, 41(12):277-284. http://d.old.wanfangdata.com.cn/Periodical/yyxb201402008 LI X, GONG B, XU K. Effects of Exogenous Nitric Oxide on Reactive Oxygen Metabolism in Ginger Leaves Under Heat Stress[J]. Acta Horticulturae Sinica, 2014, 41(12):277-284.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201402008
[16]	付晴晴, 谭雅中, 翟衡, 等. NaCl胁迫对耐盐性不同葡萄株系叶片活性氧代谢及清除系统的影响[J].园艺学报, 2018, 45 (1):30-40. http://d.old.wanfangdata.com.cn/Periodical/yyxb201801004 FU Q Q, TAN Y Z, ZHAI H, et al.Effects of Salt Stress on the Generation and Scavenging of Reactive Oxygen Species in Leaves of Grape Strains with Different Salt Tolerance[J]. Acta Horticulturae Sinica, 2018, 45 (1):30-40.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201801004
[17]	张建光, 李英丽, 刘玉芳, 等.高温、强光对苹果树冠不同方位果皮的氧化胁迫研究[J].中国农业科学, 2004, 37(12):1976-1980. doi: 10.3321/j.issn:0578-1752.2004.12.032 ZHANG J G, LI Y L, LIU Y F, et al. Effecte of high temperature and strong light on oxidative stress of apple fruit peel on different exposures of tree crown[J]. Scientia Agricultura Sinica, 2004, 37(12):1976-1980.(in Chinese) doi: 10.3321/j.issn:0578-1752.2004.12.032
[18]	PYNGROPE S, BHOOMIKA K, DUBEY R S. Oxidative stress, protein carbonylation, proteolysis and antioxidative defense system as a model for depicting water deficit tolerance in Indica rice seedling[J]. Plant Growth Regul, 2013, 69:149-165. doi: 10.1007/s10725-012-9758-3
[19]	雷东锋, 冯怡, 蒋大宗.植物中多酚氧化酶的特征[J].自然科学进展, 2004, 14(6): 606-614. doi: 10.3321/j.issn:1002-008X.2004.06.002 LEI D F, FENG Y, JIANG D Z. Characteristics of polyphenol oxidase in plants[J]. Progress in Natural Science, 2004, 14(6): 606-614.(in Chinese) doi: 10.3321/j.issn:1002-008X.2004.06.002
[20]	孙军利, 赵宝龙, 郁松林. SA对高温胁迫下葡萄幼苗AsA-GSH循环的影响[J].核农学报, 2015, 29(4): 799-804. http://d.old.wanfangdata.com.cn/Periodical/hnxb201504024 SUN J L, ZHAO B L, YU S L.Effects of Exogenous Salicylic Acid on Ascorbate Glutathione Cycle Circulation Metabolism in Grape Seedlings Under High Temperature Stress[J].Journal of Nuclear Agricultural Sciences, 2015, 29(4): 799-804.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hnxb201504024
[21]	ANDREWS P K, JOHNSON J R. Anatomical changes and antioxidant levels in the peel of sunscald damaged apple fruit[J]. Plant Physiology, 1997, 114(3):103.