Effect of Potassium Fertilizations on Grouting and Plumpness of Tetraploid Tartary Buckwheat Grains
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摘要:
目的 四倍体苦荞充实度较差,影响到其在生产上的推广应用,研究钾肥用量对提高四倍体苦荞籽粒充实的影响,以期较大幅度地提高其产量水平。 方法 以四倍体苦荞品种TB195为研究对象,设不同钾肥用量处理:低钾(LK:12 kg·hm-2)、中钾(MK:24 kg·hm-2)、高钾(HK:48 kg·hm-2),以不施钾肥为对照(CK)进行随机区组试验,测定不同处理对四倍体苦荞籽粒灌浆动态、根系形态、籽粒充实度及产量的影响。 结果 MK处理的灌浆起始势(R0)、达最大生长速率的天数(Tmax·G)、灌浆速率最大时的生长量(Wmax·G)、根系长度、根系表面积、根系体积、籽粒充实度和产量均最大。MK处理两年平均籽粒充实度为31.63%,分别比CK处理、LK处理、HK处理增加45.22%、38.12%、23.79%;MK处理两年平均产量为1 159.3 kg·hm-2,分别比CK处理、LK处理、HK处理增产54.60%、50.19%、31.58%。不同钾肥处理在灌浆时期对粒重的贡献率总体上表现为中期最大,后期次之,前期最小。形状参数值(N)以CK处理最高,MK处理最低。 结论 适宜的钾肥施用量(24 kg·hm-2)不仅可以促进四倍体苦荞的生长发育,还有利于提高其籽粒充实度和产量。 Abstract:Objective Improvement for grouting of the commonly poorly filled grains of tetraploid tartary buckwheat by potassium fertilization was studied. Method Buckwheat TB195 was treated with varying potassium fertilization at 0 kg·hm-2 (CK), 12 kg·hm-2 (LK), 24 kg·hm-2 (MK), and 48 kg·hm-2 (HK) in a randomized block experiment. Effect of the fertilizations on grain-grouting, root morphology, grain plumpness and yield of the buckwheat plants were determined. Result Among all treatments, the initial grouting potential (R0), days to reach maximum growth rate (Tmax·G), weight at maximum grouting rate (Wmax·G), plumpness, and yield of the grains, as well as the length, surface area, and volume of the plant roots were maximized when MK was applied. The 31.63% grain-filling under MK was 45.22%, 38.12%, and 23.79% higher than those under CK, LK, and HK, respectively. The 2-year average yield of 1, 159.3 kg·hm-2 by MK was 54.60%, 50.19%, and 31.58% higher than CK, LK, and HK, respectively. The contribution rate of the various fertilizations in different grouting stages to the grain weight gain was the highest when potassium was applied during the middle period followed by the late stage, and the lowest in the beginning of grouting. The shape indicator (N) was the highest under CK, and lowest with MK treatment. Conclusion At the rate of 24 kg·hm-2, the application of potassium fertilizer not only promoted the growth and development of tetraploid tartary buckwheat plants, but also significantly increased the grouting and yield, as well as the market appeal, of the grains. -
Key words:
- tetraploid tartary buckwheat /
- potassium fertilization /
- grain-grouting /
- grain plumpness /
- yield
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表 1 不同处理对四倍体苦荞籽粒TB195增重过程的影响
Table 1. Grain dry weight as affected by potassium fertilizations on TB195
处理
Treatment百粒重100-grain weight/g 花后7 d
7 days after flowering花后14 d
14 days after flowering花后21 d
21 days after flowering花后28 d
28 days after flowering花后35 d
35 days after flowering花后42 d
42 days after floweringCK 0.1025±0.0008c 0.3899±0.0014d 0.5002±0.0004d 0.5539±0.0011d 0.8869±0.0004d 0.8973±0.0006d LK 0.1874±0.0007b 0.4957±0.0003c 0.8886±0.0009c 1.0108±0.0007c 1.3660±0.0011c 1.3659±0.0009c MK 0.2013±0.0007a 0.9215±0.0005a 1.1679±0.0004a 1.2598±0.0003b 1.8967±0.0003b 2.4159±0.0005b HK 0.1010±0.0009d 0.6898±0.0089b 1.0593±0.0005b 1.8577±0.0007a 2.5899±0.0002a 2.9754±0.0009a 注:表中同列数据后无相同小写字母者表示不同处理间在0.05水平上差异显著。表 2~6同。
Note: Data on a same column with different lowercase letters indicate significant differences at P < 0.05.The same as Table 2-6.表 2 籽粒灌浆的Richards方程参数
Table 2. Parameters in Richards equation for grain-grouting
处理
Treatment实际百粒重
Actual 100-grain weight/gA B K N R2 R0 Tmax·G/d Gmax/(g·hd-1) Wmax·G/g I /% D/d CK 0.897 1.156 0.558 0.091 0.154 0.939 0.591 14.147 0.060 0.103 39.45 47.34 LK 1.366 1.511 0.553 0.132 0.124 0.983 1.065 11.326 0.597 0.589 38.97 32.18 MK 2.416 6.494 0.050 0.041 0.015 0.948 2.733 29.365 6.542 2.407 37.05 98.29 HK 2.975 3.942 0.784 0.105 0.123 0.993 0.854 17.640 1.248 1.535 38.95 40.44 注:A为籽粒最终质量; B为初值参数; K为生长速率参数; N为形状参数; R0为灌浆起始势; Tmax·G为灌浆速率为最大时的日期; Gmax为最大灌浆速率; Wmax·G为灌浆速率最大时的生长量; I为灌浆速率为最大时的生长量与籽粒最终质量比值; D为有效灌浆期; R2(W依t的回归平方和占总平方和的比率)为判断参数, 表示其配合度。
Note: A-final weight of grain; B-initial parameter; K-growth rate; N-shape indicator; R0-initial grouting potential; Tmax·G-days to reach maximum growth rate; Gmax-maximum grouting rate; Wmax·G-weight at maximum grouting rate; I-growth/final weight ratio of grain at Gmax; D-effective grouting period; and, R2-comparitive parameter indicating correlation.表 3 籽粒灌浆阶段的划分
Table 3. Divisions of grain-grouting stages
处理
Treatmentt1/d t2/d t3/d 前期Prophase 中期Medium term 后期Late 天数
Number of/d灌浆速率
Rate/ (g·d-1)贡献率
Contribution rate/%天数
Number of/d灌浆速率
Rate/ (g·d-1)贡献率
Contribution rate/%天数
Number of/d灌浆速率
Rate/ (g·d-1)贡献率
Contribution rate/%CK 2.85 25.45 64.69 2.85 0.001 3.0 22.6 0.037 63.6 39.24 0.018 26.5 LK 3.63 19.02 46.17 3.63 0.039 9.3 15.39 0.061 47.5 27.15 0.041 31.6 MK 5.73 52.99 141.56 5.73 0.085 7.5 42.76 0.085 48.7 98.8 0.044 38.1 HK 7.97 27.31 61.45 7.97 0.046 9.3 19.34 0.124 35.8 42.11 0.082 47.6 注:(1)t1、t2分别为灌浆速率方程的2个拐点在t坐标上的值,t3为假定达99% A值(籽粒最终质量)时的实际灌浆终值期;(2)灌浆速率按每百粒计算。
Note: (1) t1 and t2 are two turning points on t coordinate of grouting rate equation; and, t3, actual grain weight reached 99% of assumed final grain weight;(2) Grouting rate at 100-grains weight.表 4 不同处理对四倍体苦荞TB195根系形态的影响
Table 4. Effect of potassium fertilizations on morphology of TB195 roots
处理
Treatment总长度
Length/cm表面积
Surface area/cm2体积
Bulk/cm3平均直径
Average diameter/mmCK 44.49±0.38d 10.65±0.07d 0.45±0.04b 0.71±0.07a LK 128.12±0.49b 16.73±0.36b 0.46±0.05b 0.42±0.07bc MK 169.72±0.48a 25.43±0.43a 0.82±0.08a 0.46±0.05b HK 113.66±0.35c 11.92±0.08c 0.24±0.06c 0.35±0.35c 表 5 不同处理对四倍体苦荞TB195农艺性状的影响
Table 5. Effect of potassium fertilizations on agronomic traits of TB195 plants
处理
Treat ment株高
Plant height /cm子叶节高度
Cotyledon node height/cm1~2节节间长度
1-to 2-internode length/cm1~2节节间粗度
1-to 2-internode diameter/mm主茎节数
Main stem node number主茎分枝数
Branch number of main stemCK 75.7±0.26c 3.3±0.20a 3.3±0.25b 4.36±1.00b 13.0±2.00a 5.00±0.10a LK 90.70±0.70b 2.20±0.45b 3.20±0.36b 4.95±2.00a 15.00±1.00a 6.00±0.05a MK 92.30±0.25a 2.80±0.06a 4.30±0.40a 5.10±2.00a 16.00±1.00a 5.00±0.07a HK 67.30±0.35d 3.30±0.25a 2.90±0.25b 3.88±3.00c 15.00±1.00a 6.00±0.14a 表 6 不同钾肥用量对四倍体苦荞TB195充实度和产量的影响
Table 6. Effect of potassium fertilizations on grain plumpness and yield of TB195 plants
年份
Year处理
Treatment充实度
Plumpness/%产量
Yield/(kg·hm-2)2016 CK 21.28±0.09d 627.40±0.45d LK 21.48±0.07c 653.00±0.25c MK 28.05±0.10a 989.20±0.50a HK 25.48±0.12b 840.20±0.55b 2017 CK 22.28±0.73c 872.30±0.70d LK 24.32±0.44b 890.80±2.90c MK 35.21±0.17a 1329.40±0.82a HK 25.61±0.45b 921.90±1.12b -
[1] 陈庆富.五个中国荞麦(Fagopyrum)种的核型分析[J].广西植物, 2001, 21(2):107-111. doi: 10.3969/j.issn.1000-3142.2001.02.005CHEN Q F. Karyotype analysis of five Fagopyrum species native to China[J]. Guangxi plant, 2001, 21(2):107-111.(in Chinese) doi: 10.3969/j.issn.1000-3142.2001.02.005 [2] 张以忠, 陈庆富.荞麦研究的现状与展望[J].种子, 2004, 23(3):39-42. http://d.old.wanfangdata.com.cn/Periodical/zhongz200403013ZHANG Y Z, CHEN Q F. Current status and prospects of buckwheat research[J]. Seed, 2004, 23(3):39-42.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zhongz200403013 [3] ZHOU X, WEN L, LI Z, et al. Advance on the benefits of bioactive peptides from buckwheat[J]. Phytochemistry Review, 2015, 14(3), 381-388. doi: 10.1007/s11101-014-9390-0 [4] 闫斐艳, 崔晓东, 李玉英, 等.苦荞麦黄酮对人食管癌细胞EC9706增殖的影响[J].中草药, 2010, 41(7):1142-1145. http://d.old.wanfangdata.com.cn/Periodical/zcy201007030YAN F Y, CUI X D, LI Y Y, et al. Effect of tartary buckwheat flavonoids on proliferation of human esophageal cancer cell line EC9706[J]. Chinese Traditional and Herbal Drugs, 2010, 41(7):1142-1145. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zcy201007030 [5] 黄凯丰, 李振宙, 王炎, 等.我国荞麦高产栽培生理研究进展[J].贵州师范大学学报(自然科学版), 2019, 37(1):115-120. http://d.old.wanfangdata.com.cn/Periodical/gzsfdxxb-zr201901020HUANG K F, LI Z Z, WANG Y, et al. Research progress on physiology of buckwheat under high-yield cultivation[J]. Journal of Guizhou Normal University (Natural Science Edition), 2019, 37(1):115-120. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gzsfdxxb-zr201901020 [6] 陈庆富.荞麦生产状况及新类型栽培荞麦育种研究的最新进展[J].贵州师范大学学报(自然科学版), 2018, 36(3):1-7. http://d.old.wanfangdata.com.cn/Periodical/gzsfdxxb-zr201803001CHEN Q F. The status of buckwheat production and recent progresses of breeding on new type of cultivated buckwheat[J]. Journal of Guizhou Normal University (Natural Science Edition), 2018, 36(3):1-7. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gzsfdxxb-zr201803001 [7] 王炜, 欧巧明, 杨随庄.苦荞麦化学成分及生物活性研究进展[J].杂粮作物, 2010, 30(6):419-423. doi: 10.3969/j.issn.2095-0896.2010.06.015WANG W, OU Q M, YANG S Z. Advances in Chemical Constitutents of Tartary Buckwheat and Its Bioactivites[J]. Multigrain Crop, 2010, 30(6):419-423.(in Chinese) doi: 10.3969/j.issn.2095-0896.2010.06.015 [8] 郎桂常.苦荞麦的营养价值及其开发应用[J].中国粮油学报, 1996, 11(3):9-14. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900446208LANG G C. Bitter Buckwheat-A Chinese Neutraceutical[J].Journal of the Chinees Cereals Oils Association, 1996, 11(3):9-14.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900446208 [9] 宋毓雪, 胡静洁, 孔德章, 等.不同氮、磷、钾水平对苦荞产量和品质的影响[J].安徽农业大学学报, 2014, 41(3):411-415. http://d.old.wanfangdata.com.cn/Periodical/ahnydxxb201403014SONG Y X, HU J J, KONG D Z, et al. Effects of different ratios of nitrogen, phosphorus and potassium on yield and quality of tatary buckwheat[J]. Journal of Anhui Agricultural University, 2014, 41(3):411-415.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ahnydxxb201403014 [10] 宋毓雪, 郭肖, 杨龙云, 等.不同氮磷钾肥料处理对苦荞籽粒充实度及产量的影响[J].浙江农业学报, 2014, 26(6):1568-1572. http://d.old.wanfangdata.com.cn/Periodical/zjnyxb201406029SONG Y X, GUO X, YANG L Y, et al. Effects of different NPK treatments on the yield and plumpness of tartary buckwheat[J]. Zhejiang Agricultural Journal, 2014, 26(6):1568-1572.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjnyxb201406029 [11] 廉立坤, 陈庆富.二倍体和四倍体苦荞种子蛋白质含量和黄酮含量比较研究[J].种子, 2013, 32(2):1-5. http://d.old.wanfangdata.com.cn/Periodical/zhongz201302001LIAN L K, CHEN Q F. A Comparative Study of Seed Protein Content and Seed Flavonoid Content between Diploid and Tetraploid Tartary Buckwheat[J]. Seed, 2013, 32(2):1-5.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zhongz201302001 [12] 杨卫兵.小麦多胺和乙烯合成对水分亏缺的响应及其与籽粒灌浆特性的关系[D].泰安: 山东农业大学, 2014.YANG W B. Polyamines and Ethylene Biosynthesis in Response to Water Deficit in Wheat and Its Relations to Grain Filling[D]. Taian: Shandong Agricultural University, 2014.(in Chinese) [13] 谭德水.长期施钾对北方典型土壤钾素及作物产量、品质的影响[D].北京: 中国农业科学院, 2007.TAN D S.Effect of Long-term Application of Potassium on Soil K. crop Yield and Quality in Selected Soils from North China[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007.(in Chinese) [14] 孔丽丽, 杨晓丹, 李前, 等.钾肥不同施用方式对春玉米钾素吸收、利用和产量的影响[J].玉米科学, 2017, 25(3):111-116. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ymkx201703019KONG L L, YANG X D, LI Q, et al. Effect of Different Potassium Fertilizer Application Methodson Potassium Absorption.Utilization and Yield of Spring Maize[J]. Journal of Maize Science, 2017, 25(3):111-116.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ymkx201703019 [15] NADIA K.不同水分条件下小麦茎秆可溶性糖和农艺性状的遗传解析[D].北京: 中国农业科学院, 2015.NADIA K.Genetic Dissection of Stem Water-Soluble Carbohydrates and Agronomic Traits in Wheat under Different Water Regimes[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015.(in Chinese) [16] 朱庆森, 曹显祖, 骆亦其.水稻籽粒灌浆的生长分析[J].作物学报, 1988, 14(3):182-193. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zuowxb198803002ZHU Q S, CAO X Z, LUO Y Q. Growth analysis on the process of grain filling in rice[J]. Acta Agronomica Science, 1988, 14(3):182-193.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zuowxb198803002 [17] 顾世梁, 朱庆森, 杨建昌, 等.不同水稻材料籽粒灌浆特性的分析[J].作物学报, 2001, 21(1):7-14. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200101002GU S L, ZHU Q S, YANG J C, et al. Analysis on Grain Filling Characteristics for Different Rice Types[J]. Acta Agronomica Science, 2001, 14(3):182-193.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb200101002 [18] 张宗文, 林汝法.荞麦种质资源描述规范和数据标准[M].北京:中国农业出版社, 2007:13-14.ZHANG Z W, LIN R F. Descriptive Norms and Data Standards for Buckwheat Germplasm Resources[M]. Beijing: China Agricultural Press, 2007, 13-14.(in Chinese) [19] 杨建昌, 朱庆森, 王志琴, 等.亚优2号结实率与谷粒充实率的研究[J].江苏农学院学报, 1994, 15(4):14-18. http://www.cnki.com.cn/Article/CJFDTotal-JSNX404.002.htmYANG J C, ZHU Q S, WANG Z Q, et al. A study on the setting percentage and grain filling percenatage of YAYOU 2[J]. Journai of Jiangsu Agricultural College, 1994, 15(4):14-18.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-JSNX404.002.htm [20] 赵海峰, 张红, 张晓翔.不同磷肥水平对高油玉米生长指标的影响[J].吉林农业科技学报, 2009, 18(3):6-8. http://d.old.wanfangdata.com.cn/Periodical/jlnykjxyxb200903003ZHAO H F, ZHANG H, ZHANG X X. On the Effect of Different P Fertilizer Level on the Growth Index of High Oil Corn[J]. Jilin Agricultural Science and Technology, 2009, 18(3):6-8.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jlnykjxyxb200903003 [21] LIANG C G, SONG Y X, GUO X, et al.Characteristics of the grain-filling process and starch accumulation of high-yield common buckwheat'cv.Fengtian 1' and tartary buckwheat'cv.Jingqiao2'[J]. Cereal Research Communication, 2016, 44(3): 393-403. doi: 10.1556/0806.44.2016.005 [22] JIA Y B, YANG X E, FENG Y, et al. Differential response of root morphology to potassium deficient stress among rice genotypes varying in potassium efficiency[J]. Zhejiang University Journal Science B: International Biomedicine & Biotechnology Journal, 2008, 9(5):427-434. http://cn.bing.com/academic/profile?id=943903808b3d0db516f613c90e38cd6d&encoded=0&v=paper_preview&mkt=zh-cn [23] ROSHANI G A, NARAYANASAMY G. Effects of potassium on temporal growth of root and shoot of wheat and its uptake in different soils[J]. International Journal of Plant Production, 2010, 4(1):25-32. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000000817474 [24] 唐小付, 龙明华, 于文进.钾对黄瓜生长发育和产量及养分吸收的影响[J].北方园艺, 2010, 4(11):5-8. http://d.old.wanfangdata.com.cn/Periodical/bfyany201011002TANG X F, LONG M H, YU W J. Effects of Potassium on Growth, Development, Yield and Minera Nutrients Absorption of Cucumber[J]. Northern Gardening, 2010, 4(11):5-8.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201011002