酸铝对杉木幼苗叶片抗氧化酶活性的影响

王培; 张家君; 刘翠; 马志慧; 陈宇; 林思祖

doi:10.19303/j.issn.1008-0384.2019.07.015

酸铝对杉木幼苗叶片抗氧化酶活性的影响

doi: 10.19303/j.issn.1008-0384.2019.07.015

王培^{1, 2,},
张家君^{1, 2},
刘翠^{1, 2},
马志慧^{2, 3},
陈宇^{1, 2, ,},
林思祖^{1, 2}

1.
福建农林大学林学院, 福建福州 350002
2.
国家林业局杉木工程技术研究中心, 福建福州 350002
3.
福建农林大学生命科学学院, 福建福州 350002

基金项目:

国家林业局杉木工程技术研究中心平台建设项目 ptjh130002

国家林业局杉木工程技术研究中心孵化基金 6213C011103

详细信息

作者简介:
王培(1993-), 女, 硕士研究生, 主要从事林木遗传育种研究(E-mail:790646055@qq.com)

通讯作者:
陈宇(1983-), 男, 主要从事林木遗传育种研究(E-mail:28811852@qq.com)

中图分类号: Q945.78;S791.27
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出版历程
- 收稿日期: 2018-11-09
- 修回日期: 2019-06-28
- 刊出日期: 2019-07-20

Physiological Response of Chinese Fir (Cunninghamia lanceolata) Seedlings Under Acid and/or Aluminum Stresses

WANG Pei^{1, 2
,},
ZHANG Jia-jun^{1, 2},
LIU Cui^{1, 2},
MA Zhi-hui^{2, 3},
CHEN Yu^{1, 2
, ,},
LIN Si-zu^{1, 2}

1.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou, Fujian 350002, China
3.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要

摘要: 目的为了探究杉木在酸铝胁迫下抗氧化酶活性的变化规律及其作用机理，以此为基础探索逆境胁迫下杉木的抗性机制。方法通过土培盆栽试验，以1年生杉木实生苗为试验对象，采用0.24 g·kg^-1的AlCl₃·6H₂O模拟铝胁迫、pH 4.0的酸液模拟酸胁迫以及两者共施模拟酸铝复合胁迫，测定幼苗叶片不同胁迫时间（15、30和45 d）的MDA含量和SOD、POD、CAT、PPO等酶活性。结果无酸无铝状态下，MDA含量均处于低水平状态，单酸、单铝和酸铝处理皆引发MDA的过量积累，且影响程度是酸铝复合胁迫>铝胁迫>酸胁迫。在单酸、单铝和酸铝处理下，发现POD和SOD酶活性的增长幅度是酸铝复合胁迫>酸胁迫>铝胁迫；CAT和PPO酶活性的增长幅度则是酸铝复合胁迫>铝胁迫>酸胁迫。随着胁迫时间延长，POD、SOD活性均先增后减；CAT活性在铝胁迫和酸铝复合胁迫下先增后减，酸胁迫下逐渐增加；PPO活性则均逐渐增加。结论单酸、单铝和酸铝处理对POD、SOD、CAT和PPO活性均产生不同程度的诱导，但同时引发MDA的积累。酸铝复合胁迫对抗氧化酶活性具有一定的协同效应，酸胁迫对POD和SOD活性的促进作用大于铝胁迫，对于CAT和PPO活性，铝胁迫的促进作用则大于酸胁迫。
- 杉木 /
- 酸铝 /
- 抗氧化酶活性 /
- MDA
Abstract: Objective The antioxidant enzyme activities and respond mechanism of Chinese fir (Cunninghamia lanceolata) under acid and/or aluminum stresses were investigated. Method A pot experiment was conducted to determine the MDA content and SOD, POD, CAT, and PPO activities in one-year-old leaves of Chinese fir seedlings under the stress for 15, 30 or 45 d. At the rate of 0.24 g·kg^-1, AlCl₃ 6H₂O was applied to the pots to simulate an aluminum stress, a pH 4.0 acid solution added to simulate an acid stress, and the combination of the two incorporated to simulate an acid-aluminum stress. Result Without the presence of acid or aluminum, the potted seedlings had a low content of MDA in the leaves. However, the addition of acid, aluminum or acid-aluminum combination induced excessive accumulation of MDA with the severities ranking as acid-aluminum stress > aluminum stress > acid stress. The addition also raised the POD and SOD activities in leaves with a rank of acid-aluminum stress > acid stress > aluminum stress, and the CAT and PPO activities in the order of acid-aluminum stress > aluminum stress > acid stress. A prolonged treatment caused an initial increase on the POD and SOD activities followed by a decline; and on the CAT activity similarly under the aluminum or acid-aluminum stress but a steady increase under the acid stress; whereas, a gradual increase on the PPO activity under any of the 3 treatments. Conclusion The acid, aluminum or acid-aluminum stress affected the POD, SOD, CAT and PPO activities in the leaves to varying extents and raised MDA accumulation in the leaves of Chinese fir seedlings. There appeared to be a synergistic effect on the activities between acid and aluminum. But alone, the acid stress exerted a greater effect on POD and SOD than did aluminum, whereas, aluminum seemed to be a more formidable adversary than acid for the seedlings on CAT and PPO.
- Chinese fir /
- acid-aluminum stress /
- oxidase activity /
- MDA

HTML全文

图 1 酸铝胁迫下幼苗MDA含量的变化

注：小写字母表示同一时间不同处理间的差异显著性，数字表示同一处理不同时间间的差异显著性(P < 0.05)，图 2~5同。

Figure 1. Changes on MDA content in seedlings under acid-aluminum stress

Note:Lowercase letters indicate significant differences between different treatments in the same period, the numbers indicate the significant difference between different periods of the same treatment (P < 0.05).The same as Fig. 2-5.

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图 2 酸铝胁迫下POD酶活性的变化

Figure 2. Changes on POD activity in seedlings under acid-aluminum stress

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图 3 酸铝胁迫下SOD酶活性的变化

Figure 3. Changes on SOD activity in seedlings under acid-aluminum stress

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图 4 酸铝胁迫下CAT酶活性的变化

Figure 4. Changes on CAT activity in seedlings under acid-aluminum stress

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图 5 酸铝胁迫下PPO化酶活性的变化

Figure 5. Changes on PPO activity in seedlings under acid-aluminum stress

下载: 全尺寸图片幻灯片

表 1 试验处理

Table 1. Experimental design

编号 Numbers	Al含量 Al content/(g·kg^-1)	pH值 pH value
处理1Treatment 1	0.00	6.8(dH₂O)
处理3Treatment 3	0.24	6.8(dH₂O)
处理2Treatment 2	0.00	4.00
处理4Treatment 4	0.24	4.00
注：表中的Al表示的是AlCl₃·6H₂O的重量；dH₂O为蒸馏水。 Note：Al in the table indicates the weight of AlCl₃·6H₂O; dH₂O is distilled water.

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表 2 不同土层土壤的物理性质

Table 2. Physical properties of soil in different ground layers

土层 Soil layer/cm	土壤容重 Bulk density/(g·cm^-1)	毛管孔隙 Capillary pore/%	最大持水量 Maximum water-holding capacity/(g·kg^-1)	最小持水量 Minimum water-holding capacity/(g·kg^-1)	毛管持水量 Capillary moisture capacity/(g·kg^-1)
0~20	0.913	42.07	586.07	372.32	503.17
20~40	0.944	39.78	567.47	381.23	532.36
40~60	0.952	38.96	558.72	383.46	556.07

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表 3 不同土层土壤的养分含量

Table 3. Nutrient contents of soil in different ground layers

土层 Soil layer/cm	有机质 Organic matter/(g·kg^-1)	全氮 Total N/(g·kg^-1)	全磷 Total P /(g·kg^-1)	水解氮 Hydrolytic N /(g·kg^-1)	速效磷 Available P /(g·kg^-1)
0~20	4.12	0.284	0.257	21.87	26.73
20~40	2.28	0.207	0.197	18.72	19.79
40~60	1.76	0.167	0.169	13.16	14.37

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