稀酸提取羊栖菜多糖的结构及其抗氧化特性研究

吴娟; 欧志荣; 李昭蓉; 赵谋明

doi:10.19303/j.issn.1008-0384.2019.07.014

稀酸提取羊栖菜多糖的结构及其抗氧化特性研究

doi: 10.19303/j.issn.1008-0384.2019.07.014

华南理工大学食品科学与工程学院, 广东广州 510640

基金项目:

国家自然科学基金项目 31871746

国家自然科学基金项目 31701539

详细信息

作者简介:
吴娟(1995-), 女, 硕士研究生, 研究方向:食品生物技术(E-mail:wuyajuan512@163.com)

通讯作者:
赵谋明(1964-), 男, 教授, 研究方向:食品生物技术(E-mail:femmzhao@scut.edu.cn)

中图分类号: R284.2
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出版历程
- 收稿日期: 2019-02-12
- 修回日期: 2019-05-14
- 刊出日期: 2019-07-20

Structure and Antioxidant Activity of Polysaccharides Extracted from Sargassum fusiforme

School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China

摘要

摘要: 目的详细探讨了不同醇沉条件制备的羊栖菜多糖组分的单糖组成、平均分子量、结构及其抗氧化特性。方法通过稀酸提取法制备羊栖菜提取液，用不同浓度的乙醇沉淀制备羊栖菜多糖组分。利用柱前衍生-反相高效液相色谱、凝胶渗透色谱与多角度激光光散射联用、傅里叶变换红外光谱、紫外分光光度计、扫描电子显微镜以及哈克MARS Ⅲ型流变仪等技术手段，分析了多糖组分的基本成分、单糖组成、平均分子量、有机官能团、空间构象、表观形貌及流变学特性。此外，进一步研究了各多糖组分的氧自由基吸收能力以及DPPH·和ABTS·的清除能力。结果 SFP-50和SFP-70具有高的总糖、硫酸基及糖醛酸含量；5种多糖组分均由甘露糖、葡萄糖、葡萄糖醛酸、半乳糖、阿拉伯糖和岩藻糖组成，但占比均不相同，其中岩藻糖占比都最高，约为50%；SFP-40、SFP-50、SFP-60、SFP-70和SFP-80的平均分子量分别为97.63、90.51、97.19、81.52及90.93 kDa；流变试验表明羊栖菜多糖溶液属于非牛顿流体，表现为固体弹性性质；扫描电镜图像揭示羊栖菜多糖组分表面呈凹凸不平、疏松多孔的褶皱结构；抗氧化特性分析结果表明羊栖菜多糖组分具有一定的氧自由基吸收能力以及DPPH·和ABTS·的清除能力，且不同体积分数乙醇醇沉会影响其抗氧化特性。结论不同体积分数乙醇醇沉会影响羊栖菜多糖的理化性质、单糖组成和平均分子量，但不改变其构象、微观结构及流变学性质，对其抗氧化活性也有一定的影响。
- 羊栖菜 /
- 多糖 /
- 结构表征 /
- 抗氧化活性
Abstract: Objective The polysaccharides extracted from Sargassum fusiforme with different ethanol volume were studied. Method Polysaccharides were extracted from S.fusiforme using an acid solution, then precipitated with different volume fractions of ethanol. The resulting extract was analyzed for its physicochemical properties, monosaccharide composition, molecular weight, structural characteristics, functional group identification, spatial conformation, apparent morphology, and rheological properties using ultraviolet spectrophotometer, precolumn derivatization-reverse-phase high performance liquid chromatography, GPC-MALLs, FT-IR, SEM, and Huck MARS Ⅲ rheometer. The antioxidant activity of the extracts, including the oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability, were determined. Result Extracts, SFP-50 and SFP-70, were found to contain higher contents of sugar, sulfate and uronic acid than the other samples. All 5 extracts contained mannose, glucose, glucuronic acid, galactose, arabinose, and fucose but differed in ratio, with fucose being the highest at about 50% of them combined. The molecular weights of SFP-40, SFP-50, SFP-60, SFP-70, and SFP-80 were estimated to be 97.63, 90.51, 97.19, 81.52, and 90.93 kDa, respectively. The Congo red assay showed that the polysaccharides had a triple helical structure. The solution of the extracts was non-Newtonian fluid with elasticity. The scanning electron microscopy images showed an uneven, loose and porous surface. All samples had varying degrees of oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability. Conclusion The volume fraction employed for the ethanol precipitation affected the physicochemical properties, monosaccharide composition, and molecular weight as well as the antioxidant activity, but not the conformation, microstructure or rheological properties, of the polysaccharides obtained.
- Sargassum fusiforme /
- polysaccharides /
- structural characterization /
- antioxidant activity

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图 1 羊栖菜多糖的红外光谱

Figure 1. Infrared spectrum of polysaccharides extracted from S. fusiforme

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图 2 多糖-刚果红复合物在不同浓度NaOH溶液中最大吸收波长变化

Figure 2. Changes on maximum absorption wavelength of polysaccharides-Congo red complex in NaOH solution of different concentrations

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图 3 羊栖菜多糖的扫描电镜图

Figure 3. Scanning electron micrograph of polysaccharides extracted from S. fusiforme

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图 4 羊栖菜多糖的动态储能模量(G′)和损耗模量(G″)以及稳定剪切流变曲线

Figure 4. Dynamic energy storage modulus (G'), loss modulus (G"), and constant shear rheological curve of S.fusiforme polysaccharides

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图 5 羊栖菜多糖的ORAC值

注：图中不同小写字母表示差异显著(P < 0.05)。图 6~7同。

Figure 5. ORAC value of polysaccharides extracted from S. fusiforme

Note:Different lowercase letters in the figure indicated significant differences (P < 0.05).The same as Fig. 6-7.

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图 6 羊栖菜多糖的DPPH·清除率

Figure 6. DPPH·scavenging rate of polysaccharides extracted from S. fusiforme

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图 7 羊栖菜多糖的ABTS·的清除率

Figure 7. ABTS·scavenging rate of polysaccharides extracted from S. fusiforme

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表 1 羊栖菜多糖的理化性质

Table 1. Physicochemical properties of polysaccharides extracted from S. fusiforme

项目Item	SFP-40	SFP-50	SFP-60	SFP-70	SFP-80
总糖Total sugar/%	69.71±2.72a	78.24±1.90c	71.73±2.55ab	74.70±2.62bc	68.48±1.87a
总蛋白Total protein/%	0.72±0.06a	0.64±0.07a	0.74±0.14a	0.76±0.19a	0.68±0.07a
硫酸基Sulfate/%	1.67±0.65a	7.05±1.02c	4.62±0.93b	7.18±1.76c	4.23±0.56b
糖醛酸Uronic acids/%	37.63±2.28bc	39.45±0.86c	32.96±2.2a	38.87±1.35c	35.81±0.04b
注：同行数据后不同小写字母表示差异显著(P < 0.05)。 Note：Different lowercase letters after the same line of data indicated significant difference (P < 0.05).

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表 2 羊栖菜多糖的分子量及单糖组成

Table 2. Molecular weight and monosaccharide composition of polysaccharides extracted from S. fusiforme

处理 Treatment	单糖的摩尔百分比Molar percentage of monosaccharide/%							平均分子质量 Mw/kDa
处理 Treatment	甘露糖 Man	鼠李糖 Rha	葡萄糖醛酸 Glc A	葡萄糖 Glc	半乳糖 Gal	阿拉伯糖 Ara	岩藻糖 Fuc	平均分子质量 Mw/kDa
SFP-40	8.96	0.00	10.63	2.46	14.47	12.37	51.10	97.63
SFP-50	16.05	0.00	10.51	2.23	13.18	5.70	52.33	90.51
SFP-60	14.12	1.42	9.01	4.26	12.24	4.63	54.32	97.19
SFP-70	13.12	1.33	12.13	5.78	14.87	3.02	49.75	81.52
SFP-80	11.56	0.67	9.67	8.65	12.22	3.43	53.79	90.93

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