福建省番鸭小鹅瘟流行毒株病原学研究

林锋强; 程晓霞; 朱小丽; 陈少莺; 江丹丹

doi:10.19303/j.issn.1008-0384.2019.07.007

福建省番鸭小鹅瘟流行毒株病原学研究

doi: 10.19303/j.issn.1008-0384.2019.07.007

林锋强^{1, 2},
程晓霞^{1, 2},
朱小丽^{1, 2, ,},
陈少莺^{1, 2},
江丹丹¹

1.
福建省农业科学院畜牧兽医研究所, 福建福州 350013
2.
福建省畜禽疫病防治工程技术研究中心, 福建福州 350013

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2016R1022-5

详细信息

作者简介:
林锋强(1976-), 男, 硕士, 副研究员, 主要从事畜禽传染病防控研究

通讯作者:
朱小丽(1979-), 女, 助理研究员, 主要从事畜禽传染病和免疫学研究(E-mail:lfq1976@163.com)

中图分类号: S855
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- 被引次数: 0
出版历程
- 收稿日期: 2019-04-22
- 修回日期: 2019-06-25
- 刊出日期: 2019-07-20

An Etiological Study on Muscovy Duck-Origin Goose Plague in Fujian

LIN Feng-qiang^{1, 2},
CHENG Xiao-xia^{1, 2},
ZHU Xiao-li^{1, 2
, ,},
CHEN Shao-ying^{1, 2},
JIANG Dan-dan¹

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350013, China
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

摘要

摘要: 目的旨在研究福建省番鸭小鹅瘟病流行毒株及病毒遗传变异状况。方法从福建省番鸭主要饲养区收集临床疑似番鸭小鹅瘟病例30份，进行病原学检测、病原分离、病毒感染试验、基因片段序列分析。结果显示30份疑似病例中有15份为番鸭源鹅细小病毒（Muscovy duck-origin goose parvovirus，MDGPV）感染，占比50%。分离到的15株MDGPV均能致死番鸭胚，番鸭感染后发病率为60%~100%，死亡率为40%~60%。15株MDGPV分离毒VP1基因核苷酸同源性在99.5%以上，与MDGPV-PT株同源性在96%以上，未发生碱基缺失、插入或基因重组，较为稳定；而与番鸭细小病毒MDPV-P株同源性小于90%。进化树分析显示15株分离毒与MDGPV-PT株属于同一分支，而与MDPV-P株属于不同分支。结论福建省2018年流行的MDGPV毒株与1997年分离的MDGPV-PT株致病性和基因序列特性相似，无明显变化，生物学特性和基因组特性比较稳定，遗传变异程度小。
- 番鸭小鹅瘟 /
- 番鸭源GPV /
- 流行毒株 /
- 病原
Abstract: Objective Etiology of the epidemic Muscovy duck-origin goose plague (MDGP) and genetic variation of the pathogenic parvovirus (MDGPV) in Fujian were studied. Method Clinic specimens from 30 suspected MDGP cases at the major duck breeding areas in Fujian were collected for the etiological detection, pathogen isolation, virus infection test, and gene fragment sequence analysis. Result On the 30 cases, 15 were confirmed to be MDGPV-positive. All isolated 15 MDGPV could cause death to the Muscovy duck embryos. The morbidity and mortality on the ducks from the viral infection were 60%-100% and 40%-60%, respectively. The nucleotide homology of VP1 gene of the MDGPV isolates was greater than 99.5%. The homology of the isolates with MDGPV-PT was higher than 96% with no deletion and insertion on the nucleobases or gene recombination. On the other hand, its homology with MDPV-P was less than 90%. The phylogenetic tree analysis showed that the 15 isolates belonged to the same branch as MDGPV-PT but differed from MDPV-P. Conclusion The pathogenicity and gene sequence of the MDGPV strain prevalent in 2018 and those of the MDGPV-PT isolated in 1998 were similar with no apparent mutations. The biological and genomic characteristics of the MDGPV isolates seemed stable with little genetic variation throughout the years.
- muscovy duck-origin goose plague /
- muscovy duck derived goose parvovirus /
- prevalent strain /
- pathogen

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图 1 临床病料胰腺荧光检测结果

注：A为MDGPV阴性病料，B为MDGPV阳性病料。

Figure 1. Fluorescence test results on clinical materials

Note:A:MDGPV negative, B:MDGPV positive

下载: 全尺寸图片幻灯片

图 2 15株MDGPV分离株进化树

Figure 2. Evolutionary trees of 15 MDGPV isolates

下载: 全尺寸图片幻灯片

表 1 MDGPV分离株尿囊液病毒检测结果

Table 1. Allantoic fluid test results on MDGPV isolates

毒株代号 Strain code	MDGPV-FA	MDPV-FA	DHV-PCR	MDRV-PCR	NDRV-PCR
MDGPV-01D3	+++	-	-	-	-
MDGPV-03D3	++	-	-	-	-
MDGPV-05D2	++	-	-	-	-
MDGPV-07D2	++	-	-	-	-
MDGPV-09D2	++	-	-	-	-
MDGPV-XWD2	+++	-	-	-	-
MDGPV-FTD2	++	-	-	-	-
MDGPV-QSD1	+++	-	-	-	-
MDGPV-02D2	+++	-	-	-	-
MDGPV-04D2	+++	-	-	-	-
MDGPV-06D2	+++	-	-	-	-
MDGPV-08D3	+++	-	-	-	-
MDGPV-XYD2	++	-	-	-	-
MDGPV-LCD1	++	-	-	-	-
MDGPV-THD3	+++	-	-	-	-
注：表中“++”表示1个视野可见3~5个以上荧光灶，+++表示1个视野可见5个以上荧光灶。 Note：++means that more than 3-5 fluorescent foci can be seen in one field of vision, +++ means that more than 5 fluorescent foci can be seen in one field of vision.

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表 2 MDGPV分离株尿囊液LPA毒价结果

Table 2. Virulence of MDGPV isolates on LPA in allantoic fluid

毒株代号 Strain code	MDGPV-01D3	MDGPV-02D2	MDGPV-03D3	MDGPV-04D2	MDGPV-05D2	MDGPV-06D2	MDGPV-07D2	MDGPV-08D3	MDGPV-09D2	MDGPV-XYD2	MDGPV-XWD2	MDGPV-LCD1	MDGPV-FTD2	MDGPV-THD3	MDGPV-QSD1
LPA效价 LPA titer	2⁴	2⁵	2⁵	2⁶	2⁵	2⁵	2⁵	2⁵	2⁵	2⁶	2⁵	2⁶	2⁴	2⁴	2⁴

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表 3 番鸭感染MDGPV分离株发病和死亡结果

Table 3. Morbidity and mortality of Muscovy ducks artificially infected with MDGPV isolates

组别 Group	接种数 number of Inoculation	发病数 Number of Incidence	死亡数 Number of deaths
MDGPV-01D3	5	4	3
MDGPV-03D3	5	4	2
MDGPV-05D2	5	3	2
MDGPV-07D2	5	5	3
MDGPV-09D2	5	4	2
MDGPV-XWD2	5	5	3
MDGPV-FTD2	5	4	2
MDGPV-QSD1	5	4	2
MDGPV-PTD3	5	4	2
MDGPV-02D2	5	5	2
MDGPV-04D2	5	4	2
MDGPV-06D2	5	4	2
MDGPV-08D3	5	4	2
MDGPV-XYD2	5	5	2
MDGPV-LCD1	5	4	2
MDGPV-THD3	5	3	2
对照组Control	5	0	0

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