日粮营养水平对育肥苏姜猪血清生化指标和肉质相关基因的影响

周根来; 陶勇; 倪黎纲; 任善茂; 徐盼; 殷洁鑫; 张君胜; 周春宝; 赵旭庭

doi:10.19303/j.issn.1008-0384.2019.06.007

日粮营养水平对育肥苏姜猪血清生化指标和肉质相关基因的影响

doi: 10.19303/j.issn.1008-0384.2019.06.007

周根来^{1, 2,},
陶勇^{1, 3},
倪黎纲¹,
任善茂¹,
徐盼¹,
殷洁鑫¹,
张君胜¹,
周春宝¹,
赵旭庭^1, ,

1.
江苏农牧科技职业学院, 江苏泰州 225300
2.
江苏省现代畜牧与新兽药工程技术中心, 江苏泰州 225300
3.
江苏苏姜种猪有限公司, 江苏泰州 225300

基金项目:

国家发展和改革委员会生物育种能力建设与产业化专项项目发改办高技（2014）2573号

江苏省农业三新工程项目 SXGC（2017）188

江苏农牧科技职业学院项目 NSF201602

详细信息

作者简介:
周根来(1979-), 男, 硕士, 副教授, 研究方向:猪营养生理与饲料资源开发(E-mail:glarechou@hotmail.com)

通讯作者:
赵旭庭(1966-), 男, 硕士, 教授, 研究方向:猪品种资源开发与保护(E-mail:jsfdyc@163.com)

中图分类号: S828.2
计量
- 文章访问数: 899
- HTML全文浏览量: 253
- PDF下载量: 16
- 被引次数: 0
出版历程
- 收稿日期: 2019-04-04
- 修回日期: 2019-05-05
- 刊出日期: 2019-06-28

Serum Biochemistry and Expression of Carcass Trait-related Gene of Sujiang Pigs as Affected by Dietary Makeup at Final Growth Stage

1.
Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu 225300, China
2.
Jiangsu Provincial Engineering Technology Center of Modern Animal Science and New Veterinary Drugs, Taizhou, Jiangsu 225300, China
3.
Jiangsu Sujiang Breeding Pig Co., Ltd., Taizhou, Jiangsu 225300, China

摘要

摘要: 目的研究日粮不同消化能（DE）、粗蛋白（CP）和粗纤维（CF）水平对育肥阶段苏姜猪血清生化指标和肉质相关基因的影响。方法选用162头体重（50.49±4.78）kg的苏姜猪，设定不同DE（11.64、12.24、12.84 MJ·kg^-1）、CP（12%、13%、14%）及CF（5%、8%、11%）水平，采用L₉（3⁴）正交试验设计，设置9组日粮处理，每组3个重复，每个重复6头（公母各半）。预饲期7 d，试验期42 d。结果（1）血清生化指标：12.84 MJ·kg^-1 DE组总蛋白含量显著高于11.64 MJ·kg^-1 DE组（P < 0.05）；12.84 MJ·kg^-1DE组球蛋白含量均极显著高于11.64、12.24 MJ·kg^-1 DE组（P < 0.01）；8%、11% CF组尿氮含量分别极显著（P < 0.01）、显著（P < 0.05）高于5% CF组；13%、14%两CP组总胆固醇含量极显著低于12% CP组（P < 0.01），14% CP组甘油三酯含量极显著低于12% CP组（P < 0.01）；11% CF组葡萄糖含量极显著低于5% CF组（P < 0.01），12.84 MJ·kg^-1 DE组肌酐含量显著低于11.64 MJ·kg^-1 DE组（P < 0.05）。（2）血清激素及酶活：12.84 MJ·kg^-1 DE组血清胰岛素水平显著高于11.64 MJ·kg^-1 DE组（P < 0.05），11% CF组血清中三碘甲腺原氨酸含量显著低于5% CF组（P < 0.05）。日粮营养水平对试验猪血清中各酶活没有显著影响（P>0.05）。（3）肉质相关基因：11.64 MJ·kg^-1 DE组背最长肌H-FABP、PRAKG3基因的表达量显著高于12.24、12.84 MJ·kg^-1两DE组（P < 0.05），HSL基因表达量极显著高于12.24、12.84 MJ·kg^-1两DE组（P < 0.01）；日粮CP和CF水平对试验猪各基因表达均没有显著影响（P>0.05）。结论日粮中不同DE、CP和CF水平可通过调控血清生化指标和肉质相关基因表达影响育肥苏姜猪的生长发育，育肥苏姜猪适宜的DE、CP和CF水平分别为12.84 MJ·kg^-1、12%、11%。
- 苏姜猪 /
- 日粮营养水平 /
- 生化指标 /
- 激素水平 /
- 酶活 /
- 基因表达
Abstract: Objective Effects of diet composition on growth performance, serum biochemistry and carcass trait-related gene expression of Sujiang pigs at final stage prior to slaughtering were investigated. Method Randomly selected 162 Sujiang pigs with a body weight of (50.49±4.78)kg were divided in 9 groups with 3 replicates per group and 6 pigs per replicate (half male and half female) for the study. The diets used in the L₉(3⁴) orthogonal experiment were varied with 3 levels of digestible energy (DE) at 11.64, 12.24, and 12.84 MJ·kg^-1, 3 levels of crude protein (CP) at 12%, 13%, and 14%, and 3 levels of crude fiber (CF) at 5%, 8%, and 11%. A pre-feeding period lasted 7 d followed by 42-d-feeding before sampling for serum analysis on the pigs. Result (1) The TP content in the 12.84 MJ·kg^-1 DE group was significantly higher than that in the 11.64 MJ·kg^-1 DE group (P < 0.05), and GLB in the 12.84 MJ·kg^-1 DE groups were significantly higher than those in the 11.64 and 12.24 MJ·kg^-1 DE groups (P < 0.01). UN in the 8% CF and the 11% CF groups were extremely significantly (P < 0.01) and significantly (P < 0.05) higher than that in the 5% CF group. TC in the 14% CP groups were significantly lower than that in the 12% CP group (P < 0.01), and TG in the 14% CP group was significantly lower than that in the 12% CP group (P < 0.01). Glu in the 11% CF group was significantly lower than that in the 5% CF group (P < 0.01), and CREA in the 12.84 MJ·kg^-1 DE group was significantly lower than that in the 11.64 MJ·kg^-1 DE group (P < 0.05). (2) The serum insulin in the 12.84 MJ·kg^-1 DE group was significantly higher than that in the 11.64 MJ·kg^-1 DE group (P < 0.05). Serum T3 in the 11% CF group was significantly lower than that in the 5% CF group (P < 0.05). However, the dietary nutrition showed no significant effect on serum enzyme activities in the pigs (P>0.05). (3) The expressions of H-FABP and PRAKG3 genes in the longissimus dorsi muscle of the 11.64 MJ·kg^-1 DE group were significantly higher than those in the muscle of the 12.24 and 12.84 MJ·kg^-1 DE groups (P < 0.05), while that of HSL gene was significantly higher than those of 12.24 and 12.84 MJ·kg^-1 DE groups (P < 0.01). The dietary CP or CF induced no significant effect on the expression of the genes in the pigs (P>0.05). Conclusion Dietary DE, CP and CF affected the growth and development of Sujiang pigs at the final fattening stage through regulating the serum biochemistry and carcass trait-related gene expression of the animals. It appeared that a provision of DE at 12.84 MJ·kg^-1, CP at 12%, and CF at 11% in the forage was appropriate for the pigs in that stage.
- sujiang pigs /
- dietary nutrient level /
- biochemistry /
- hormone /
- enzyme activity /
- gene expression

HTML全文

图 1 日粮消化能水平对育肥苏姜猪基因表达的影响

注：同一项目中，数据柱上标不同大写字母表示差异极显著(P < 0.01)，上标不同小写字母表示差异显著(P < 0.05)，无上标或上标相同字母表示差异不显著(P>0.05)。图 2、3同。

Figure 1. Effect of dietary DE levels on mRNA expression of Sujiang pigs at fattening stage

Note: In the same item, values with different capital letter superscripts mean significant difference (P < 0.01), and with different small letter superscripts mean significant difference (P < 0.05), while with no letter or the same letter superscripts mean no significant difference (P>0.05). The same as Fig. 2, 3.

下载: 全尺寸图片幻灯片

图 2 日粮粗蛋白水平对育肥苏姜猪基因表达的影响

Figure 2. Effect of dietary CP levels on mRNA expression of Sujiang pigs at fattening stage

下载: 全尺寸图片幻灯片

图 3 日粮粗纤维水平对育肥苏姜猪基因表达的影响

Figure 3. Effect of dietary CF levels on mRNA expression of Sujiang pigs at fattening stage

下载: 全尺寸图片幻灯片

表 1 目的基因及β-actin的引物序列

Table 1. Primer sequences of targeted genes and β-actin

基因 Gene	引物序列(5′-3′) Primer sequence	片段长度 Length(bp)
H-FABP	F/R：CACAAAGCACCTTCAAGAGCAC/GGGTGAGTGTCAGGATGAGTTTC	198
PRKAG3	F/R：CCTCTACCGCACCATCCAAG/CCAGTTTCGTTGACCACAGG	141
AMPD1	F/R：AAATCTTACCAAATTGATGCCG/CAACAGTCAGGTCGTAGGGATG	112
HSL	F/R：CGAGATTAGCACCAAGGAGGAG/CATGAAGGGATTCTTGACGATG	136
LPL	F/R：AACTTGGGCTATGAGATCAATAAGG/CGCATCTTTACTTGGTAATGGAAG	110
β-actin	F/R：GCAAGGACCTCTACGCCAA/CTGGAAGGTGGACAGCGAG	191

下载: 导出CSV

表 2 育肥苏姜猪血清常规生化指标

Table 2. Serum biochemistry of Sujiang pigs at fattening stage

项目 Items	水平 Level	总蛋白 TP/(g·L^-1)	白蛋白 ALB/(g·L^-1)	球蛋白 GLB/(g·L^-1)	尿素氮 UN/(mmol·L^-1)	总胆固醇 TC/(mmol·L^-1)	甘油三酯 TG/(mmol·L^-1)	葡萄糖 Glu/(mmol·L^-1)	肌酐 CREA/(μmol·L^-1)
消化能DE/(MJ·kg^-1)	11.64	68.02±6.41b	42.79±3.30	25.84±4.31Bb	4.48±1.23	2.67±0.49	0.45±0.17	3.13±0.50	93.20±13.67a
	12.24	69.60±4.22ab	43.31±3.36	26.30±3.08Bb	4.42±1.40	2.88±0.51	0.44±0.12	3.14±0.61	85.34±14.15ab
	12.84	72.54±6.98a	42.18±4.55	29.76±5.09Aa	3.57±1.38	2.63±0.52	0.47±0.16	3.42±0.79	83.76±9.07b
粗蛋白CP/%	12	69.94±6.18	42.33±4.12	27.61±3.56	4.48±1.15	3.08±0.49Aa	0.54±0.16Aa	3.08±0.57	87.94±13.91
	13	71.21±8.00	43.09±4.43	28.11±6.19	3.93±1.83	2.65±0.51Bb	0.45±0.15ABab	3.37±0.81	86.73±14.43
	14	69.02±3.73	42.84±2.59	26.17±3.25	4.06±1.04	2.45±0.32Bb	0.36±0.09Bb	3.24±0.52	87.63±11.00
粗纤维CF/%	5	69.24±4.39	41.93±2.87	27.30±4.04	3.42±1.34Bb	2.55±0.41	0.41±0.12	3.54±0.76Aa	86.07±7.85
	8	69.87±7.91	42.46±3.96	27.41±5.91	4.68±1.46Aa	2.78±0.63	0.46±0.17	3.21±0.55ABab	87.33±16.86
	11	71.06±5.92	43.87±4.18	27.19±3.52	4.38±1.05ABa	2.86±0.43	0.48±0.15	2.94±0.48Bb	88.90±13.17
注：同列数据后不同大、小写字母表示同一处理不同水平间差异极显著(P < 0.01)和差异显著(P < 0.05)。表 3~4同。 Note：In the same column, values with different capital letter superscripts mean significant difference (P < 0.01), and with different small letter superscripts mean significant difference (P < 0.05). The same as Table 3-4.

下载: 导出CSV

表 3 育肥苏姜猪血清激素水平

Table 3. Serum hormone level of Sujiang pigs at fattening stage

项目 Items	水平 Level	三碘甲腺原氨酸(T₃) /(pmol·L^-1)	甲状腺素(T₄) /(μg·L^-1)	生长激素(GH) /(μg·L^-1)	胰岛素Insulin /(mIU·L^-1)	胰岛素样生长因子-I IGF-I/(μg·L^-1)
消化能DE/(MJ·kg^-1)	11.64	230.64±44.99	234.02±38.45	24.29±4.63	72.43±13.12b	16.86±3.02
	12.24	241.88±43.32	248.89±32.11	21.86±4.24	81.68±14.73ab	18.46±3.24
	12.84	241.80±49.45	241.92±40.81	24.17±5.04	83.88±13.24a	16.72±3.39
粗蛋白CP/%	12	240.19±39.88	239.10±31.15	22.99±3.77	78.05±14.60	17.77±3.61
	13	235.81±47.51	235.69±32.85	24.96±4.75	84.73±16.21	17.40±2.37
	14	238.31±50.76	250.04±46.08	22.38±5.31	75.21±10.78	16.88±3.74
粗纤维CF/%	5	246.71±42.03a	245.29±38.17	23.96±5.05	80.45±10.73	16.51±2.94
	8	250.89±38.95a	238.91±39.13	23.48±4.76	77.88±16.82	17.60±3.43
	11	216.71±49.14b	240.62±35.78	22.90±4.47	79.66±15.51	17.94±3.39

下载: 导出CSV

表 4 育肥苏姜猪血清酶活

Table 4. Serum enzyme activity of Sujiang pigs at fattening stage

项目 Items	水平 Level	谷丙转氨酶(ALT) /(mU·L^-1)	谷草转氨酶(AST) /(U·L^-1)	胆碱酯酶(CHE) /(IU·L^-1)	碱性磷酸酶(ALP) /(U·L^-1)
消化能DE/(MJ·kg^-1)	11.64	242.87±48.12	38.07±6.63	378.24±72.81	160.35±25.97
	12.24	241.74±46.39	36.06±7.47	401.88±63.74	150.06±26.61
	12.84	245.02±38.60	36.80±7.90	424.71±68.50	144.18±34.49
粗蛋白CP/%	12	240.38±48.10	37.16±7.47	381.04±78.91	150.08±30.34
	13	237.94±43.92	37.78±8.11	414.32±70.40	151.70±32.12
	14	251.30±40.17	35.99±6.41	409.47±57.72	152.82±27.53
粗纤维CF/%	5	241.22±43.24	35.84±7.13	420.11±68.35	153.79±30.25
	8	238.78±43.40	36.61±7.86	397.59±76.35	142.74±25.52
	11	249.62±46.28	38.48±6.92	387.13±64.02	158.07±31.86

下载: 导出CSV

参考文献(34)

[1]	杨彩梅.日粮能量、蛋白水平对断奶仔猪生长性能、血液生化指标及激素水平的影响[J].中国畜牧杂志, 2005, 41(9):31-32. http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200509013 YANG C M. Effects of dietary energy and protein levels on growth performance, blood biochemical indices and hormone levels of weaned piglets[J].Chinese Journal of Animal Science, 2005, 41(9):31-32.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200509013
[2]	霍永久, 刘正旭, 方伟, 等.日粮纤维水平对育肥淮猪血清生化指标及相关基因表达的影响[J].扬州大学学报(农业与生命科学版), 2015, 36(4):27-30, 44. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jsnyyj201504007 HUO Y J, LIU Z X, FANG W, et al.Effect of dietary fiber on serum biochemistry and related gene expression of fattening Huai pigs[J].Journal of Yangzhou University(Agricultural and Life Science Edition), 2015, 36(4):27-30, 44.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jsnyyj201504007
[3]	罗献梅, 陈代文, 余冰, 等.营养水平对DLY杂交猪肉质性状及H-FABP基因表达的影响[J].中国畜牧杂志, 2008, 44(19):26-29. http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200819008 LUO X M, CHEN D W, YU B, et al.Effects of nutrition levels on heart fatty acid- binding protein gene expression and fatty metabolism in muscle of pigs[J].Chinese Journal of Animal Science, 2008, 44(19):26-29.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200819008
[4]	李梦云, 余冰, 张克英, 等.营养水平对PRKAG3基因表达量及对肉质影响的研究[J].畜牧兽医学报, 2008, 39(8):1056-1061. doi: 10.3321/j.issn:0366-6964.2008.08.009 LI M Y, YU B, ZHANG K Y, et al. Effect of nutritional level on PRKAG3 gene expression and pork quality[J].ActaVeterinariaetZootechnicaSinica, 2008, 39(8):1056-1061.(in Chinese) doi: 10.3321/j.issn:0366-6964.2008.08.009
[5]	刘作华, 陈代文, 龙定彪, 等.日粮能量水平对猪脂肪酸合成酶和激素敏感酯酶的影响[J].中国畜牧杂志, 2009, 45(5):31-33, 55. http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200905008 LIU Z H, CHEN D W, LONG D B, et al. Effect of dietary energy levels on the activity and mRNA expression of fatty acid synthase and hormone-sensitive lipase in pigs[J]. Chinese Journal of Animal Science, 2009, 45(5):31-33, 55.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgxmzz200905008
[6]	马现永, 周桂莲, 林映才, 等.饲粮中添加谷氨酸钠对黄羽肉鸡生长性能及肉品风味的影响[J].动物营养学报, 2011, 23(3):410-416. doi: 10.3969/j.issn.1006-267x.2011.03.009 MA X Y, ZHOU G L, LIN Y C, et al.Effects of sodium glutamate supplementation on growth performance and meat flavor of yellow-feathered broilers[J].Chinese Journal of Animal Nutrition, 2011, 23(3):410-416.(in Chinese) doi: 10.3969/j.issn.1006-267x.2011.03.009
[7]	李秀宝, 张恒博, 黄郁萱, 等.营养水平对保育期美系长白猪血清生化指标的影响[J].畜牧与兽医, 2010, 42(7):21-25. http://d.old.wanfangdata.com.cn/Periodical/xmysy201007006 LI X B, ZHANG H B, HUANG Y X, et al.Effects of dietary energy, crude protein, calcium and phosphorus on the serum biochemical parameters in conservation stage of Landrace pigs in USA[J].Animal Husbandry & Veterinary Medicine, 2010, 42(7):21-25.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xmysy201007006
[8]	霍永久, 占今舜, 余同水, 等.饲粮不同蛋白水平对淮猪生长性能、肉品质和血清生化指标的影响[J].草业学报, 2015, 24(6):133-141. http://d.old.wanfangdata.com.cn/Periodical/caoyexb201506016 HUO Y J, ZHAN J S, YU T S, et al.The effect of diet with different crude protein levels on growth performance, meat quality and serum parameters in Huaipigs[J].ActaPrataculturaeSinica, 2015, 24(6):133-141.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/caoyexb201506016
[9]	曹龙凯, 郭春华, 汤丽琳, 等.营养水平对贵州香猪肉质性状及H-FABP基因表达的影响[J].西南民族大学学报, 2012, 38(3):378-382. doi: 10.3969/j.issn.1003-2483.2012.03.11 CAO L K, GUO C H, TANG L L, et al.Effects of nutrition levels on meat quality traits and heart fatty acid-binding protein gene expression in Guizhou miniature pigs[J].Journal of Southwest University for Nationalities, 2012, 38(3):378-382.(in Chinese) doi: 10.3969/j.issn.1003-2483.2012.03.11
[10]	唐倩, 杨小婷, 李吕木, 等.日粮粗纤维水平对圩猪生长性能、肉品质及血液生化指标的影响[J].西北农林科技大学学报(自然科学版), 2015, 43(8):10-18. http://d.old.wanfangdata.com.cn/Periodical/xbnydxxb201508002 TANG Q, YANG X T, LI L M, et al.Effect of dietary crude fiber level on growth performance, meat quality and serum biochemical indexes of Wei pigs[J].Journal of Northwest A & F University(Natural Science Edition), 2015, 43(8):10-18.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xbnydxxb201508002
[11]	杨玉芬, 卢德勋, 许梓荣, 等.日粮纤维对于肥育猪生产性能和血清参数的影响[J].江西农业大学学报(自然科学版), 2002, 24(5):578-582. http://d.old.wanfangdata.com.cn/Periodical/jxnydxxb200205004 YANG Y F, LU D X, XU Z R, et al. A Study on the Effects of Dietary Fiber on Performance and Serum Parameters in Finishing Pigs[J].ActaAgriculturaeUniversitatis Jiang xiensis, 2002, 24(5):578-582.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jxnydxxb200205004
[12]	倪黎纲, 赵旭庭, 周春宝, 等.苏姜猪世代选育中生长、胴体和肉质性能的测定分析[J].畜牧与兽医, 2015, 47(1):45-47. http://d.old.wanfangdata.com.cn/Periodical/xmysy201501014 NI L G, ZHAO X T, ZHOU C B, et al. Determination and analysis of growth, carcass and meat quality in Sujiang pig breeding[J].Animal Husbandry & Veterinary Medicine, 2015, 47(1):45-47.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xmysy201501014
[13]	周根来, 赵旭庭, 陶勇, 等.饲粮中消化能、粗蛋白质和粗纤维水平对育肥苏姜猪生长性能、胴体性状和肉品质的影响[J].动物营养学报, 2018, 30(8):3293-3301. doi: 10.3969/j.issn.1006-267x.2018.08.049 ZHOU G L, ZHAO X T, TAO Y, et al.Effects of dietary digestible energy, crude protein and crude fiber levels on growth performance, carcass characteristic and meat quality of fattening Sujiang pigs[J].Chinese Journal of Animal Nutrition, 2018, 30(8):3293-3301.(in Chinese) doi: 10.3969/j.issn.1006-267x.2018.08.049
[14]	王娟.饲粮能量和蛋白质水平对CRP配套系商品代生长猪生产性能、消化代谢和血清生化指标的影响[D].重庆: 西南大学, 2007. http://xueshu.baidu.com/s?wd=paperuri%3A%289e1a3c87674c4c226f53a27dac5e4d02%29&filter=sc_long_sign&tn=SE_xueshusource_2kduw22v&sc_vurl=http%3A%2F%2Fcdmd.cnki.com.cn%2FArticle%2FCDMD-10635-2007132567.htm&ie=utf-8&sc_us=4333809575254798119 WANG J. Effects of dietary protein and energy levels on performance, digestibility metabolism, and serum biochemical indices in CRP complete set line's commercial growing pigs[D]. Chongqing: Southwest University, 2007.(in Chinese) http://xueshu.baidu.com/s?wd=paperuri%3A%289e1a3c87674c4c226f53a27dac5e4d02%29&filter=sc_long_sign&tn=SE_xueshusource_2kduw22v&sc_vurl=http%3A%2F%2Fcdmd.cnki.com.cn%2FArticle%2FCDMD-10635-2007132567.htm&ie=utf-8&sc_us=4333809575254798119
[15]	庄俊华.临床生化检验技术[M].北京:人民卫生出版社, 2009. ZHUANG J H. Clinical biochemical testing technology[M]. Beijing:People's Health Publishing House, 2009.(in Chinese)
[16]	CONNOLLY M A, CLAUSEN P A, LAZAR J G. Purification of RNA from animal cells using trizol[J]. CSH protocols, 2006(1).doi: 10.1101/pdb.prot4104.
[17]	郑立鑫, 刘大森, 刘化伟, 等.五种锌源对肉仔鸡生长性能、金属硫蛋白基因表达及组织锌含量的影响[J].黑龙江畜牧兽医, 2014(3):90-93. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hljxmsy201402029 ZHENG L X, LIU D S, LIU H W, et al.Effects of five zinc sources on growth performance, metallothionein gene expression and tissue zinc content in broilers[J]. Heilongjiang Animal Science and Veterinary Medicine, 2014(3):90-93.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hljxmsy201402029
[18]	潘天彪, 张家富, 肖正中, 等.不同能量、蛋白对桂科母系猪生长性能及血液生化指标的影响[J].黑龙江畜牧兽医, 2010(19):71-72. http://d.old.wanfangdata.com.cn/Conference/7452193 PAN T B, ZHANG J F, XIAO Z Z, et al.Effects of different energy and protein on growth performance and blood biochemical indices of Guike dam line pigs[J]. Heilongjiang Animal Science and Veterinary Medicine, 2010(19):71-72.(in Chinese) http://d.old.wanfangdata.com.cn/Conference/7452193
[19]	周勤飞, 王永才, 王金勇, 等.能量水平对生长猪生产性能、养分消化率和血清生化指标的影响[J].中国畜牧杂志, 2010, 46(23):44-47. http://www.cnki.com.cn/Article/CJFDTotal-ZGXM201023013.htm ZHOU Q F, WANG Y C, WANG J Y, et al. Effects of energy level on growth performance, nutrient digestibility and serum biochemical indices of growing pigs[J].Chinese Journal of Animal Science, 2010, 46(23):44-47.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-ZGXM201023013.htm
[20]	杨强, 张石蕊, 贺喜, 等.低蛋白质日粮不同能量水平对育肥猪生长性能和胴体性状的影响[J].动物营养学报, 2008, 20(4):371-376. doi: 10.3969/j.issn.1006-267X.2008.04.001 YANG Q, ZHANG S R, HE X, et al. Effects of low crude protein with different digestible energy levels on the growth performance and carcass characteristic in fattening pigs[J]. Chinese Journal of Animal Nutrition, 2008, 20(4):371-376.(in Chinese) doi: 10.3969/j.issn.1006-267X.2008.04.001
[21]	谭占坤, 张定华, 商鹏, 等.饲粮能量水平对断奶藏仔猪生产性能、养分消化率和血液生化指标的影响[J].西南农业学报, 2017, 30(7):1667-1671. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201707035 TAN Z K, ZHANG D H, SHANG P, et al. Effect of dietary energy concentration on performance, nutrient digestibility and blood biochemical parameters of Tibetan piglets[J]. Southwest China Journal of Agricultural Sciences, 2017, 30(7):1667-1671.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201707035
[22]	BHAVSAR P, RATHOD K J, RATHOD D, et al. Utility of serum creatinine, creatine kinase and urinary myoglobin in detecting acute renal failure due to rhabdomyolysis in trauma and electrical burns patients[J].Indian Journal of Surgery, 2013, 75(1):17-21. doi: 10.1007/s12262-012-0451-6
[23]	孙建武, 杨小婷, 李吕木, 等.日粮能量水平对圩猪生产性能、血清生化指标和肉质的影响[J].安徽农业科学, 2015, 43(10):134-137, 143. doi: 10.3969/j.issn.0517-6611.2015.10.051 SUN J W, YANG X T, LI L M, et al. Effect of dietary digestible energy levels on growth performance, meat quality trait and serum biochemical parameter in Weipigs[J]. Journal of Anhui Agricultural Science, 2015, 43(10):134-137, 143.(in Chinese) doi: 10.3969/j.issn.0517-6611.2015.10.051
[24]	刘立文.日粮能量水平对育成柴鸡促性腺激素及胰岛素浓度的影响[J].河南农业科学, 2014, 43(2):123-125. doi: 10.3969/j.issn.1004-3268.2014.02.028 LIU L W. Effects of diet energy level on gonadotropins, insulin of breeding domestic Chicks[J]. Journal of Henan Agricultural Sciences, 2014, 43(2):123-125.(in Chinese) doi: 10.3969/j.issn.1004-3268.2014.02.028
[25]	于长宁, 李艳娇, 张柏林, 等.不同供能物质组成饲粮对育肥猪养分表观消化率、血清生化指标和激素水平的影响[J].动物营养学报, 2015, 27(3):902-909. doi: 10.3969/j.issn.1006-267x.2015.03.030 YU C N, LI Y J, ZHANG B L, et al. Effects of different consists of dietary energy supply on nutrient apparent digestibility, serum biochemical indices and hormones levels of finishing pigs[J].Chinese Journal of Animal Nutrition, 2015, 27(3):902-909.(in Chinese) doi: 10.3969/j.issn.1006-267x.2015.03.030
[26]	曹向阳, 康相涛, 田亚东, 等.长期饲喂高纤维饲粮对固始鸡血清生化指标、屠宰性能和肉品质的影响[J].江苏农业科学, 2010(6):301-305. doi: 10.3969/j.issn.1002-1302.2010.06.121 CAO X Y, KANG X T, TIAN Y D, et al. Effects of high-fiber diet for long term on serum biochemical parameters, carcass traits and meat quality in Gushi chicken[J]. Jiangsu Agricultural Sciences, 2010(6):301-305.(in Chinese) doi: 10.3969/j.issn.1002-1302.2010.06.121
[27]	GERBENS F, JANSEN A, ERP A J, et al. The adipocyte fatty acid-binding protein locus:characterization and association with intramuscular fat content in pigs[J]. Mammalian Genome, 1998, 9(12):1022-1026. doi: 10.1007/s003359900918
[28]	TOWLE H C, KAYTOR E N, SHIH H M. Regulation of the expression of lipogenic enzyme genes by carbohydrate[J]. Annual Review of Nutrition, 2003, 17(1):405-433.
[29]	孙瑞萍, 魏立民, 刘圈炜, 等.日粮营养水平对临高猪H-FABP基因表达量和肉品质的影响[J].河南农业科学, 2015, 44(10):141-145. http://d.old.wanfangdata.com.cn/Periodical/hnnykx201510032 SUN R P, WEI L M, LIU Q W, et al. Effect of dietary nutrition levels on H-FABP gene expression and pork quality in Lingao pigs[J].Journal of Henan Agricultural Sciences, 2015, 44(10):141-145.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hnnykx201510032
[30]	AI H, IHLEMANN J, HELLSTEN Y, et al. Effect of fiber type and nutritional state on AICAR and contraction stimulated glucose transport in rat skeletal muscle[J]. American Journal of Physiology-Endocrinology and Metabolism, 2002, 282(6):E1291-1300. doi: 10.1152/ajpendo.00167.2001
[31]	LARGE V, ARNER P, REYNISDOTTIR S, et al. Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells[J]. Journal of Lipid Research, 1998, 39:1688-1695. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=236c3f9f905ab26b5a74e4974e5957aa
[32]	MEAD J R, IRVINE S A, RAMJI D P. Lipoprotein lipase:structure, function, regulation, and role in disease[J]. Journal of Molecular Medicine, 2002, 80:753-769. doi: 10.1007/s00109-002-0384-9
[33]	ZHANG B Z, LEI M G, DENG C Y, et al. Association between PCR-RFLP polymorphism of the fifth intron in lipoprotein lipase gene and productive traits in pig resource family[J]. Asian-Australasian Journal of Animal Sciences, 2005, 18(18):458-462. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JAKO200510103408298
[34]	黄英, 李永能, 杨明华, 等.日粮能量水平对乌金猪肝脏组织脂类代谢相关基因表达的影响[J].云南农业大学学报, 2012, 27(6):826-832. doi: 10.3969/j.issn.1004-390X(n).2012.06.010 HUANG Y, LI Y N, YANG M H, et al. Effect of dietary energy levels on the expression levels of hepatic lipid metabolism genes in Wujin pigs[J]. Journal of Yunnan Agricultural University, 2012, 27(6):826-832.(in Chinese) doi: 10.3969/j.issn.1004-390X(n).2012.06.010