Disease-Resistance of Shaanxi Brassica napus to Various Plasmodiophora brassicae
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摘要:
目的 为明确陕西省不同地区油菜根肿菌的致病性,筛选抗根肿病的油菜种质资源。 方法 采用根部注射的室内接种发病方法,对陕西省4个不同地区油菜根肿菌的致病性和15份甘蓝型油菜品种的根肿病抗性进行鉴定。 结果 来源于陕西省西乡县、南郑县、勉县和太白县的油菜根肿菌的致病性存在显著差异,其中勉县根肿菌的致病性最强,南郑县根肿菌的致病性最弱。参试的15个油菜品种的抗性存在显著差异,引进的5个抗性资源华杂62R、华双5R、1628A×根抗、171R和172R的根肿病抗性显著高于陕西省的10个栽培品种。聚类分析结果显示引进的5个抗性资源聚为抗病一类,陕西省的10个栽培品种聚在感病一类。 结论 陕西省4个地区根肿菌存在生理小种差异。引进的5个抗根肿病资源对这4个地区根肿菌均表现为较强的抗性,可以作为当地根肿病病区主栽品种及育种的抗性资源。 Abstract:Objective Pathogenicity of various strains of Plasmodiophora brassicae on Brassica napus cultivars was investigated. Method Four different P. brassicaes strains were collected from the counties of Xixiang, Nanzheng, Mian, and Taibai in Shaanxi, and the disease-resistance of 15 varieties of B. napus toward them by inoculating in the roots determined in an indoor test. Result The pathogenicity of the various strains were significantly different.The P. brassicaes collected from Mian county was the strongest among all, while from Nanzheng county the weakest. The disease-resistance of the 15 rapeseed varieties differed significantly as well. The 5 significantly more resistant varieties, 62R, Huashuang 5R, 1628A×genkang, 171R, and 172R, were not native Shaanxi cultivars. The cluster analysis classified them as disease-resistant toward the clubroot disease and the 10 local germplasms disease-sensitive. Conclusion Physiological differences existed among the strains of P. brassicaes from the 4 regions in Shaanxi. The 5 identified clubroot disease-resistant rapeseed cultivars would be promising candidates for breeding new varieties for cultivation in the province. -
Key words:
- Brassica napus /
- clubroot disease /
- Plasmodiophora brassicae
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表 1 15个油菜品种对4个地区根肿菌的抗性结果
Table 1. Disease-resistance of 15 rapeseed varieties to P. brassicae collected from 4 counties in Shaanxi
品种
Variety太白县Taibai county 西乡县Xixiang county 南郑县Nanzheng county 勉县Mian county A/% B C A/% B C A/% B C A/% B C 华杂62R Huaza62R 61.1 36.4 R 38.9 19.1 HR 36.1 13.3 HR 55.6 34.6 R 华双5R Huashuang5R 5.6 1.9 HR 2.8 2.2 HR 0.0 0.0 HR 38.9 14.8 HR 1628A×根抗1628A×genkang 8.3 2.8 HR 8.3 4.6 HR 0.0 0.0 HR 47.2 20.7 HR 171R 19.4 4.6 HR 0.0 0.0 HR 0.0 0.0 HR 33.3 16.0 HR 172R 5.6 1.2 HR 11.1 1.9 HR 0.0 0.0 HR 33.3 20.4 HR 渝油21 Yuyou21 86.1 57.7 S 91.7 52.8 S 63.9 31.8 R 86.1 60.8 S 赣油杂6号Ganyouza No.6 88.9 56.8 S 80.6 48.5 LR/LS 61.1 36.4 R 100.0 72.8 S 绵新油38 Mianxinyou38 86.1 58.3 S 91.7 57.7 S 69.4 37.3 R 100.0 74.1 S 蓉油18 Rongyou18 80.6 47.8 LR/LS 94.4 63.0 S 86.1 47.2 LR/LS 100.0 73.5 S 福油518 Fuyou518 66.7 41.4 LR/LS 83.3 54.3 S 72.2 30.9 R 91.7 67.0 S 沣油737 Fengyou737 100.0 74.7 S 97.2 74.4 S 88.3 48.8 LR/LS 100.0 76.5 S 秦优7号Qinyou No.7 100.0 82.1 HS 97.2 76.2 S 88.6 63.8 S 97.2 75.0 S 陕油28 Shanyou28 100.0 70.4 S 91.7 69.4 S 97.2 68.8 S 100.0 77.8 S 秦优10号Qinyou No.10 97.2 72.5 S 94.7 67.4 S 83.3 40.7 LR/LS 100.0 79.0 S 油研52 Youyan52 97.2 75.6 S 91.7 75.0 S 94.4 63.6 S 100.0 78.4 S 注:A为发病率,B为病情指数,C为抗性。
Note:A.Incidence,B.Disease index,C.Resistant.表 2 不同地区菌种间致病性差异显著性分析
Table 2. Pathogenicity of P. brassicae collected from 4 counties in Shaanxi
地区
Region发病率Incidence 病情指数Disease index 均值
Mean5%显著水平
Significant in 5%1%极显著水平
Significant in 1%均值
Mean5%显著水平
Significant in 5%1%极显著水平
Significant in 1%勉县Mian county 78.9 a A 56.1 a A 太白县Taibai county 66.9 b B 45.6 b B 西乡县Xixiang county 65.0 b B 44.4 b B 南郑县Nanzheng county 56.1 c C 32.2 c C 表 3 不同油菜品种根肿病抗性差异显著性分析
Table 3. Disease-resistance of 15 rapeseed cultivars
地区
Region发病率Incidence 病情指数Disease index 均值
Mean5%显著水平
Significant in 5%1%极显著水平
Significant in 1%均值
Mean5%显著水平
Significant in 5%1%极显著水平
Significant in 1%陕油28 Shanyou28 97.2 a A 71.6 ab AB 沣油737 Fengyou737 96.4 ab A 68.6 ab AB 油研52 Youyan52 95.9 ab A 73.2 a AB 秦优7号Qinyou No.7 95.8 ab A 74.3 a A 秦优10号Qinyou No.10 93.8 ab AB 64.9 b BC 蓉油18 Rongyou18 90.3 abc ABC 57.9 c CD 绵新油38 Mianxinyou38 86.8 bcd ABC 56.9 cd CDE 赣油杂6号Ganyouza No.6 82.6 cd BC 53.6 cde DE 渝油21 Yuyou21 81.9 cd BC 50.8 de DE 福油518 Fuyou518 78.5 d C 48.4 e E 华杂62R Huaza62R 47.9 e D 25.9 f F 1628Rx根抗1628A×genkang 16.0 f E 7.0 g G 171R 13.2 f E 5.2 g G 172R 12.5 f E 5.9 g G 华双5R Huashuang5R 11.8 f E 4.7 g G -
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