Effects of Plant Growth Regulators on Germination of Black Wheat Seeds under Salt-stress
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摘要:
目的 探讨α-萘乙酸钠(NAA)、赤霉素(GA3)和6-苄氨基嘌呤(6-BA)3种植物生长调节剂不同质量浓度对不同程度盐胁迫条件下黑小麦种子萌发的影响,为在盐渍地区种植黑小麦提供可能性和科学依据。 方法 以紫麦一号的种子为试验材料,采用纸床法分别测定不同质量浓度的3种植物生长调节剂对不同程度盐胁迫条件下黑小麦种子萌发的影响。 结果 在无盐胁迫条件下,NAA、GA3、6-BA质量浓度分别为150 mg·L-1、GA3 200 mg·L-1和6-BA 5 mg·L-1时,黑小麦种子的发芽率、发芽势等萌发指标均为最佳;复合盐(NaCl:Na2SO4=1:1)溶液浸种显著抑制了黑小麦种子的萌发;用不同质量浓度的植物生长调节剂预处理均可在一定程度上缓解复合盐胁迫对黑小麦种子萌发的抑制作用;在中低浓度(40~80 mmol·L-1)复合盐胁迫条件下,GA3 200 mg·L-1和6-BA 5 mg·L-1预处理均能有效缓解复合盐胁迫对种子萌发的影响;随着复合盐浓度的增加,各处理种子的发芽势和发芽率明显下降,当复合盐浓度为120 mmol·L-1时,只有6-BA 5 mg·L-1预处理对缓解胁迫有一定作用。 结论 在中低复合盐浓度胁迫条件下,采用GA3(200 mg·L-1)或6-BA(5 mg·L-1)预处理能够缓解盐胁迫对黑小麦种子的伤害,从而显著促进在盐胁迫条件下黑小麦种子的萌发。 Abstract:Objective Effects of 3 plant growth regulators on the germination of black wheat seeds under salt-stress were studied to provide possible relief for the wheat planting in saline-alkali areas. Method Using a paper bedding method, 1-N aphthaleneacetic acid (NAA), gibberellic acid (GA3) or 6-benzylamino-purine (6-BA) was applied on seeds of black wheat (Zimai 1) to observe the germination under varied salt concentrations. Result The best rate, potential and other indices on the seed germination were found when 150 mg·L-1 NAA, 200 mg·L-1 GA3 or 5 mg·L-1 6-BA was used without salt-stress. The presence of NaCl:Na2SO4=1:1 significantly inhibited the germination. Addition of the plant growth regulators in different concentrations relieved the inhibitive effect to a certain extent. Both GA3 at 200 mg·L-1 and 6-BA at 5 mg·L-1 significantly alleviated the germination inhibition when medium to low salt-stress (i.e., 40-80 mmol·L-1) was imposed on the seeds. The germination tendency and rate were significantly decreased with increasing salt-stress. When the salt concentration was raised to 120 mmol·L-1, only the application of 6-BA 5 mg·L-1 exhibited an effect on the germination. Conclusion The treatment of GA3 200 mg·L-1 or 6-BA 5 mg·L-1 could protect the black wheat seeds from being permanently harmed by a medium-to-low salt-stress and improve the germination. -
Key words:
- black wheat seed /
- germination /
- hormone /
- compounded salt /
- stress
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表 1 植物生长调节剂的质量浓度梯度设计
Table 1. Experimental design for applications of plant growth regulators in varied concentrations
植物生长调节剂
Plant growth regulator质量浓度梯度
Concentration gradient/(mg·L-1)NAA 50 100 150 200 250 GA3 100 150 200 250 300 6-BA 1 5 10 15 20 表 2 NAA、GA3、6-BA对黑小麦种子萌发的影响
Table 2. Effects of NAA, GA3 and 6-BA on black wheat seed germination
植物生长调节剂
Plant growth regulator浓度
Concentration / (mg·L-1)发芽率
Germination rate /%发芽势
Germination energy/%地上部长度
Above ground part/cm根长
Length of root/cmNAA CK 72.67±4.17 Ccd 56.00±4.00 Cd 4.52±0.56 Bc 5.67±0.21 Aa 50 70.00±4.00 Cd 58.67±4.16 Cd 8.13±0.57 Ab 2.15±0.25 Cd 100 86.67±3.06 Aa 71.33±1.15 Bc 7.90±0.72 Ab 2.88±0.17 BCc 150 87.33±2.31 Aa 85.33±4.17 Aa 9.27±0.67 Aa 3.50±0.20 Bb 200 82.00±2.00 ABb 78.00±3.46 ABb 9.35±0.71 Aa 2.42±0.57 Ccd 250 77.33±1.15 Bc 74.67±5.03 Bb 7.93±0.54 Ab 1.20±0.20 De GA3 CK 72.67±4.17 Bc 56.00±4.00 Cd 4.52±0.56 Cd 5.67±0.21 Dd 100 91.33±5.03 Aa 82.67±2.31ABab 7.60±0.97 Bc 7.41±0.54 Cc 150 90.00±5.29 Aa 84.33±2.04 ABb 9.71±0.36 Ab 7.52±0.41 BCc 200 91.33±3.06 Aa 87.33±3.06 Aa 10.35±0.50 Aa 9.69±0.45 Aa 250 78.67±1.15 Bb 76.25±3.06 Bb 10.26±0.62 Aa 8.35±0.16 Bb 300 76.25±1.08 Bb 63.33±5.77 Cc 7.97±0.77 Bc 5.63±0.23 Dd 6-BA CK 72.67±4.17 Bc 56.00±4.00 Cc 4.52±0.56 BCc 5.67±0.21 Aa 1 88.67±3.06 Ab 86.02±1.84 Aa 5.51±0.03 ABb 3.46±0.47 Cc 5 90.67±1.16 Aa 87.15±2.10 Aa 5.98±0.41 Aa 3.42±0.38 Cc 10 86.00±2.00 Ab 84.00±2.00 Aa 4.96±0.48 ABCab 4.07±0.21 BCbc 15 87.33±2.31 Ab 66.00±2.00 Bb 4.25±0.40 CDa 4.60±0.26 Bb 20 84.67±4.16 Ab 70.00±4.00 Bb 3.69±0.29 Dd 3.53±0.42 Cb 注:同列数据中不同大、小写字母分别表示在0.01、0.05水平上的差异显著性(Duncan′s法)。
Note: Data with different capital and lowercase letters on a same column represent extremely significant difference at 0.01 and significant difference at 0.05 level,respectively (Duncan′s).表 3 植物生长调节剂对盐胁迫抑制黑小麦种子萌发的缓解效果
Table 3. Relief effect of plant growth regulators on black wheat seed germination inhibited by salt-stress
复合盐浓度
Concentration of complex salt / (mmol·L-1)植物生长调节剂
Plant growth regulator发芽率
Germination rate /%发芽势
Germination energy /%相对盐害率
Relative injury rate /%地上部长度
Above ground part/cm根长
Length of root/cm0(CK) 无 72.67±4.17 56.00±4.00 0 4.52±0.56 5.67±0.21 40 无 56.00±2.00 Cc 18.00±3.46 Cc 22.94 4.35±0.43 BCc 4.54±0.25 Bb NAA 64.00±4.00 Bb 18.00±2.00 Cc - 4.04±0.30 Cc 3.24±0.19 Cc GA3 87.33±3.06 Aa 34.00±3.46 Bb - 7.31±0.27 Aa 8.43±0.36 Aa 6-BA 85.33±1.15 Aa 60.00±5.29 Aa - 5.02±0.20 Bb 3.31±0.36 Cc 80 无 21.33±4.16 Cd 4.00±2.00 Bc 70.65 1.84±0.28 Cd 2.32±0.24 Bc NAA 30.00±4.00 Bc 0±0.00 Bd - 2.00±0.22 Cc 2.25±0.27 Bc GA3 44.00±4.00 Ab 13.33±3.06 Ab - 5.91±0.47 Aa 5.34±0.55 Aa 6-BA 54.67±5.03 Aa 19.33±4.16 Aa - 4.93±0.27 Bb 2.77±0.25 Bb 120 无 14.00±1.25 Bc 4.00±0.00 Bb 80.73 1.63±0.25 Cc 1.56±0.30 Bc NAA 14.00±3.46 Bc 0±0.00 Cc - 1.35±0.12 Cc 1.32±0.29 Bc GA3 20.67±3.06 Bb 14.00±4.00 Aa - 2.67±0.32 Bb 3.37±0.23 Aa 6-BA 44.00±2.00 Aa 10.67±3.06 ABa - 3.93 ±0.32 Aa 2.02±0.16 Bb 160 无 4.00±2.00 Bb 4.00±2.00 ABab 94.50 0.67±0.17 Bc 0.91±0.06 Bc NAA 3.33±1.15 Bb 0±0.00 Bc - 0.92±0.15 Bb 1.06±0.11 Bc GA3 11.33±3.06 Aa 6.00±2.00 Aa - 0.72±0.10 Bc 1.83±0.25 Aa 6-BA 12.00±3.46 Aa 1.33±1.15 Bb - 1.33±0.11 Aa 1.41±0.22 ABb 200 无 0 0 100 0 0 注:(1)同列数据中不同大、小写字母分别表示在0.01、0.05水平上的差异显著性(Duncan′s法)。(2)NAA、GA3、6-BA的质量浓度分别为150 mg·L-1、200 mg·L-1、5 mg·L-1。
Note: (1) Data with different capital and lowercase letters on a same column represent extremely significant difference at 0.01 and significant difference at 0.05 level,respectively (Duncan′s). (2) Concentrations of NAA,GA3 and 6-BA are 150 mg·L-1,200 mg·L-1 and 5 mg·L-1,respectively. -
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