山药块茎生长期5种内源激素含量变化对块茎膨大的影响

敖兰吉亚; 季祥; 邵盈; 王志敏; 赵令敏; 张艳芳; 霍秀文

doi:10.19303/j.issn.1008-0384.2019.03.005

山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

内蒙古农业大学农学院/内蒙古野生蔬菜种质资源与创新重点实验室, 内蒙古呼和浩特 010019

基金项目:

国家自然科学基金项目 31360480

详细信息

作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

中图分类号: S632.1
计量
- 文章访问数: 1847
- HTML全文浏览量: 371
- PDF下载量: 34
- 被引次数: 0
出版历程
- 收稿日期: 2018-12-30
- 修回日期: 2019-01-25
- 刊出日期: 2019-03-28

Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

College of Agriculture, Inner Mongolia Agricultural University/Inner Mongolia Wild Vegetable Germplasm Resources and Innovation Key Laboratory, Hohhot, Inner Mongolia 010019, China

摘要

摘要: 目的探讨山药不同生长发育阶段主要内源激素含量的变化规律，以及不同山药品种形态指标与5种内源激素的相关性，为进一步研究山药生长发育的生理机制和指导山药的高产优质栽培奠定基础。方法以毕克齐山药和河北安平白山药7个不同生长发育时期块茎为材料，采用酶联免疫吸附法分别测定了脱落酸（ABA）、赤霉素（GA₃）、生长素（IAA）、茉莉酸（JA）、玉米素（ZR）等5种内源激素含量，并分析不同生长期内源激素含量的动态变化，以及山药形态指标与内源激素的相关性。结果在两个山药品种块茎膨大的生长发育过程中，内源激素IAA和ABA含量总体呈逐渐上升的趋势；内源激素ZR和GA₃含量有变化，但比较稳定；内源激素JA含量，两个品种均呈下降趋势，尤其是河北安平白山药的JA含量与形态指标呈极显著的负相关，说明JA明显抑制了河北安平白山药块茎的周长增加、重量增加及伸长生长；内源激素IAA和JA在不同生长期的含量水平高低，两个品种表现出不同的变化趋势。结论内源激素ABA、GA₃、IAA可促进2个山药品种块茎生长发育，其中ABA、IAA对毕克齐山药块茎伸长的促进作用更显著；而内源激素ZR、JA可促进河北安平白山药的块茎形成及初期块茎周长的增加。
- 山药 /
- 块茎膨大周期 /
- 内源激素 /
- 形态指标 /
- 相关性
Abstract: Objective Physiology relating to the hormones of two varieties of Dioscorea opposite was studied for cultivation improvement. Method The endogenous ABA, GA₃, IAA, JA, and ZR in slim-type Inner Mongolia Bikeqi yam and short-type Hebeianpingbai yam were determined by using ELISA at 7 tuber development stages. The dynamic changes of the hormones were correlated with the morphological indicators of the tuber growth. Result For both varieties, IAA and ABA increased gradually but not significantly during the tuber development. ZR and GA₃ did not change significantly. And, JA declined showing retarded extensions on tuber diameter, weight and length in Hebeianpingbai yam. Conclusion ABA, GA₃ and IAA promoted the tuber development on both varieties; ABA and IAA enhanced the tuber elongation on Inner Mongolia Bikeqi; while ZR and JA stimulated the tuber formation and diameter increase on Hebeianpingbai yam at the beginning stage.
- Dioscorea opposite Thunb. /
- tuber development /
- endogenous hormones /
- relevance /

HTML全文

图 1 ABA在山药不同生长期含量的变化

Figure 1. Changes on ABA at various growth stages of yams

下载: 全尺寸图片幻灯片

图 2 GA₃在山药不同生长期含量的变化

Figure 2. Changes on GA₃ at various growth stages of yams

下载: 全尺寸图片幻灯片

图 3 IAA在山药不同生长期含量的变化

Figure 3. Changes on IAA at various growth stages of yams

下载: 全尺寸图片幻灯片

图 4 JA在山药不同生长期含量的变化

Figure 4. Changes on JA at various growth stages of yams

下载: 全尺寸图片幻灯片

图 5 ZR在山药不同生长期含量的变化

Figure 5. Changes on ZR at various growth stages of yams

下载: 全尺寸图片幻灯片

表 1 不同山药品种在相同生长发育时期5种内源激素的差异

Table 1. Variations on 5 endogenous hormones in D. opposite at a same development stage

生长期 Growth period	品种 Varieties	ABA/ (ng·g^-1)	GA₃/ (ng·g^-1)	IAA/ (ng·g^-1)	JA/ (ng·g^-1)	ZR/ (ng·g^-1)
块茎膨大始期 Initial period of tuber expansion	河北安平白山药 Hebeianpingbai yam	39.70±1.89bB	5.46±0.35aA	32.09±1.88bA	24.70±0.56aA	7.06±0.29aA
	毕克齐山药 Bikeqi yam	55.83±3.13aA	5.33±0.22aA	35.94±0.90aA	19.92±0.48bB	5.80±0.37bA
块茎膨大初期 The beginning of tuber expansion	河北安平白山药 Hebeianpingbai yam	32.78±1.53bB	4.39±0.10bA	40.52±0.97aA	20.35±0.12bB	10.86±0.53aA
	毕克齐山药 Bikeqi yam	39.21±1.07aA	5.86±0.39aA	27.44±0.60bB	37.97±1.93aA	6.53±0.23bB
气生块茎期 Air tuber stage	河北安平白山药 Hebeianpingbai yam	38.01±1.78aA	5.12±0.23aA	27.90±1.11bA	17.64±0.28bB	5.77±0.23bB
	毕克齐山药 Bikeqi yam	32.70±0.80bA	5.59±0.36aA	31.60±1.22aA	20.78±0.46aA	7.95±0.16aA
块茎膨大中期 Intermediate period of tuberexpansion	河北安平白山药 Hebeianpingbai yam	34.30±0.97bA	5.56±0.26aA	33.78±0.95bB	11.70±0.38bB	6.66±0.25aA
	毕克齐山药 Bikeqi yam	38.518±1.76aA	5.99±0.30aA	39.28±1.24aA	20.83±0.73aA	7.15±0.26aA
块茎膨大盛期 The vigorous period of tuber expansion	河北安平白山药 Hebeianpingbai yam	30.06±2.20bB	5.08±0.24aA	36.19±1.16aA	11.25±0.57aA	7.10±0.44aA
	毕克齐山药	43.71±2.27aA	3.87±0.09bB	36.07±1.30aA	10.81±0.25aA	5.68±0.35bA
块茎膨大后期 The later period of tuber expansion	河北安平白山药 Hebeianpingbai yam	54.98±3.48aA	4.97±0.26aA	55.76±2.93aA	10.85±0.17bB	7.50±0.34aA
	毕克齐山药 Bikeqi yam	43.20±1.47bA	4.03±0.12bA	35.40±0.96bB	15.90±0.53aA	6.49±0.48bA
成熟收获期 Mature harvest period	河北安平白山药 Hebeianpingbai yam	70.95±4.13bB	5.03±0.30bA	41.02±1.94bB	12.20±0.51aA	5.81±0.26bB
	毕克齐山药 Bikeqi yam	100.33±7.53aA	5.76±0.28aA	68.23±1.24aA	12.42±0.53aA	7.42±0.11aA
注：表中数据后无相同小写字母者表示在P＜0.05水平上差异显著; 无相同大写字母者表示在P < 0.01水平上差异极显著。 Note: Data with different lowercase letters indicate significant differences at P < 0.05; data with different capital letters indicate extremely significant at P < 0.01.

下载: 导出CSV

表 2 河北安平白山药与5种内源激素的相关性

Table 2. Correlation between tuber growth and 5 endogenous hormones of Hebeianpingbai yam

项目 Progects	块茎长度 Tuber length	块茎周长 Tuber circumference	块茎干重 Tuber dry weight	块茎鲜重 Tuber freshweight	JA	IAA	ZR	GA₃	ABA
块茎长度 Tuber length		0.892^**	0.961^**	0.862^*	-0.951^**	0.587	-0.269	-0.109	0.389
块茎周长 Tuber circumference			0.852^*	0.898^**	-0.874^*	0.642	-0.092	-0.273	0.561
块茎干重 Tuberdry weight				0.927^**	-0.937^**	0.504	-0.447	-0.006	0.504
块茎鲜重 Tuber freshweight					-0.889^**	0.59	-0.383	0.013	0.671
JA						-0.45	0.34	-0.072	-0.312
IAA							0.3	-0.416	0.502
ZR								-0.729	-0.389
GA₃									-0.041
ABA
注：表中数据上标*表示在0.01级别(双尾)相关极显著；表示在0.05级别(双尾)相关显著。表 3同。 Note: ** represents extremely significant correlation at P < 0.01; * represents significant correlation at P < 0.05.The same as table 3.

下载: 导出CSV

表 3 毕克齐山药与5种内源激素的相关性

Table 3. Correlation between tuber growth and 5 endogenous hormones of Inner Mongolia Bikeqi yam

项目 Progects	块茎长度 Tuber length	块茎周长 Tuber circumference	块茎干重 Tuber dry weight	块茎鲜重 Tuber freshweight	JA	IAA	ZR	GA₃	ABA
块茎长度 Tuber length		0.558	0.653	0.636	-0.364	0.322	0.145	-0.435	0.155
块茎周长 Tuber circumference			0.854^*	0.989^**	-0.446	0.131	-0.237	-0.38	-0.059
块茎干重 Tuberdry weight				0.876^**	-0.539	0.13	-0.309	-0.665	0.028
块茎鲜重 Tuber
freshweight					-0.525	0.196	-0.256	-0.46	0.008
JA						-0.546	0.069	0.539	-0.412
IAA							0.303	0.193	0.942^**
ZR								0.588	0.119
GA₃									0.181
ABA

下载: 导出CSV

参考文献(30)

[1]	蔡金辉, 严渐子, 黄晓辉, 等.山药品种资源的分类研究[J].江西农业大学学报, 1999, 21(1): 53-56. doi: 10.3969/j.issn.1000-2286.1999.01.014 CAI J N, YAN J Z, HUANG X H, et al. Classification of yam species resources[J]. Journal of Jiangxi Agricultural University, 1999, 21(1): 53-56.(in Chinese) doi: 10.3969/j.issn.1000-2286.1999.01.014
[2]	孙霞.毕克齐长山药生育和贮藏期间营养成分及相关酶活性的研究[D].呼和浩特: 内蒙古农业大学, 2008. http://cdmd.cnki.com.cn/Article/CDMD-10129-2008131565.htm SUN X.Study on Nutrient Composition and Related Enzyme Activity during Birth and Storage of Chinese Yam[D].Hohhot: Masters Thesis, Inner Mongolia Agricultural University, 2008.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10129-2008131565.htm
[3]	YOSHIDA Y, TAKAHASHI H, KANDA H, et al.Effect of seed tuber weights on the development of tubers and flowering spikes in Japanese yams (Dioscorea japonica) grown under different photoperiods and with plant growth regulators.[J]Jpn Soc Hortic Sci, 2007, 76(3):230-236.
[4]	朱海旺, 霍秀文.长山药块茎休眠期相关酶活性及内源激素含量变化[J].华北农学报, 2011, 26(2):198-202. http://d.old.wanfangdata.com.cn/Periodical/hbnxb201102041 ZHU H W, HUO X W.Chinese Yam(Dioscorea opposite Thunb.)Dormancy-related Activity and the Dynamic Changes of Endogenous Hormone[J].Acta Agriculture Borieali-Sinica, 2011, 26(2):198-202.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnxb201102041
[5]	龙雯虹, 王琼, 肖关丽等.山药珠芽休眠期内源激素含量的变化及多效唑破除休眠的效应[J].西南农业学报, 2013, 26(5):1996-2000. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201305049 LONG W H, WANG Q, XIAO G L, et al.Variation of Endogenous Hormones in Dormant Yam Bulbils and Response of Dormant Bulbils to PP333[J].Southwest China Journal of Agricultural Sciences, 2013, 26(5):1996-2000.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201305049
[6]	龙雯虹, 郭华春, 肖关丽等.山药珠芽生长过程中激素和糖类物质含量的变化[J].园艺学报, 2011, 38(4):753-760. http://d.old.wanfangdata.com.cn/Periodical/yyxb201104018 LONG W H, GUO H C, XIAO G L, et al.Changes of hormones and carbohydrates during the growth of yam bead buds[J]. Acta Horticulturae Sinica, 2011, 38(4):753-760.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201104018
[7]	常莉, 薛建平, 宋运贤, 等.怀山药试管块茎形成过程中内源激素的变化[J].中国中药杂志, 2010, 35(21):2818-2821. http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201021006 CHANG L, XUE J P, SONG Y X, et al.Changes of Endogenous Hormones in Tuber Formation of Chinese Yam Tuber[J].China Journal of Chinese Materia Medica.2010, 35(21):2818-2821.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201021006
[8]	WOLTERS H, JURGENS G.Survival of the flexible:hormonal growth control and adaptation in plant development[J].Nature reviews genetics, 2009, 10(5):305-317. doi: 10.1038/nrg2558
[9]	SANTER A, CALDERON-VILLALOBOS L I A, ESTELLE M.Plant hormones are versatile chemical regulators of plant growth[J].Nature Chemical Biology, 2009, 5(5):301-307. doi: 10.1038/nchembio.165
[10]	李玲玲.菊芋块茎形成及其与内源激素的关系初步研究[D].南京: 南京农业大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10307-1017042217.htm LI L L.A preliminary study on the tuber formation of Helianthus tuberosus and its relationship with endogenous hormones[D].Nanjing: Nanjing Agricultural University, 2015.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10307-1017042217.htm
[11]	萧浪涛, 王三根.植物生理学[M].北京:中国农业出版社, 2011. XIAO L T, WANG S G. Plant Physiology[M].Beijing: China Agriculture Press, 2011.(in Chinese)
[12]	周芸依, 张静, 王亚伦, 等.赤霉素调控植物块茎形态建成的研究进展[J].作物杂志, 2016(4):20-25 http://d.old.wanfangdata.com.cn/Periodical/zwzz201604004 ZHOU Y Y, ZHANG J, WANG Y L.Progress of Gibberellin Regulation on Tuber Morphogenesis in Higher Plant[J]. .Crops, 2016(4):20-25.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwzz201604004
[13]	王志敏.山药(Dioscorea opposita)块茎不同发育时期相关差异基因的cDNA-AFLP分析[D].呼和浩特市: 内蒙古农业大学, 2018. http://cdmd.cnki.com.cn/Article/CDMD-10129-1018881920.htm WANG Z M.Analysis of Tubers Development Related Differential ExpressionGenes by c DNA-AFLP in Yam(Dioscoreaopposita)[D].Hohhot: Inner Mongolia Agricultural University, 2008.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10129-1018881920.htm
[14]	甘立军, 曾晓春, 周燮.茉莉酸类与植物地下贮藏器官的形成[J].植物学通报, 2001, 18(5): 546-553. doi: 10.3969/j.issn.1674-3466.2001.05.005 GAN L J, ZENG X C, ZHOU Y.Formation of Jasmonic Acids and Underground Storage Organs in Plants[J].Chinese Bulletin of Botany, 2001, 18(5):546-553.(in Chinese) doi: 10.3969/j.issn.1674-3466.2001.05.005
[15]	汪雷.钙对半夏生理生化的影响及探究茉莉酸在半夏珠芽中的差异表达[D].杭州: 浙江理工大学, 2018. http://cdmd.cnki.com.cn/Article/CDMD-10338-1018192377.htm WANG L.Effects of calcium on physiological and biochemicalcharacteristicsofPinelliaternata and the differential expression of Jasmonic acid inPinellia bulbils[D].HangZhou: Zhejiang Sci-Tech University, 2018.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10338-1018192377.htm
[16]	孙鹏.植物激素对甜菜块根增长和糖分积累的调控作用[D].呼和浩特: 内蒙古农业大学, 2014. SUN P.The Regulation of Plant Hormone on the Increase of Tuber Weight and Sugar Accumulation of Sugar Beet[D].Hohhot: Inner Mongolia Agricultural University, 2008.(in Chinese)
[17]	周生茂.山药不同基因型地下块茎糖类和酚类物质形成、调控及相关基因分离的研究[D].杭州: 浙江大学, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10335-2009156630.htm ZHOU S M.Studies on biosyntheses, regulations, and gene isolations involved in carbohydrates and phenols in underground tubers of different genotypes of yams[D].HangZhou: Zhe Jiang University, 2008.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-10335-2009156630.htm
[18]	张岩荣, 董诚明, 薛南南, 等.不同等级山药种栽与其内源激素(IAA、GA₃)含量及蔗糖酶活性相关性研究[J].中国现代中药, 2017, 19(3):393-397. http://d.old.wanfangdata.com.cn/Periodical/zyyjyxx201703017 ZHANG Y R, DONG C M, XUE N N, et al.Cultivation of Different Grade Yam and Correlation between Endogenous Hormone (IAA, GA₃) Content and Invertase Activity[J].Modern Chinese Medicine, 2017, 19(3):393-397.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zyyjyxx201703017
[19]	许超.莲藕膨大过程中生理生化变化的研究[D].扬州: 扬州大学, 2002. http://cdmd.cnki.com.cn/Article/CDMD-11117-2003123116.htm XU C.Study on Physiological and Biochemical Changes during the Expansion of Lotus Root[D]. YangZhou: Yang Zhou University, 2002.(in Chinese) http://cdmd.cnki.com.cn/Article/CDMD-11117-2003123116.htm
[20]	薛建平, 葛德燕, 张爱民, 等.试管地黄的不定根膨大过程中4种内源激素的消长[J].作物学报, 2004, 30(10):1056-1059. doi: 10.3321/j.issn:0496-3490.2004.10.017 XUE J P, GE D Y, ZHANG A M, et al.The growth and decline of four endogenous hormones in the process of adventitious root swelling of Dioscoreazingiberensis[J].ActaAgronomica Sinica, 2004, 30(10):1056-1059.(in Chinese) doi: 10.3321/j.issn:0496-3490.2004.10.017
[21]	ACHARD P, GUSTI A, CHEMINANT S, et al. Gibberellin signaling control cell proliferation rate in Arabidopsis[J]. Current Biology, 2009, 19(14):1188-1193. doi: 10.1016/j.cub.2009.05.059
[22]	GEL, YONG J W H, TAN S N, et al.Analysis of some cytokinins in coconut (Cocos nucifera L.) water by micellar electrokinetic capillary after solid-phase extraction[J].Journal of Chromatography A, 2004, 1048(1):119-126. doi: 10.1016/S0021-9673(04)01186-0
[23]	KIYOSHIT, KAIENF, YOSHIOK, et al.Expansion of potato cells in response to jasmonic acid[J]. Plant Science, 1994, 100(1):3-8. doi: 10.1016/0168-9452(94)90127-9
[24]	KELEN M, DEM IRALAY E C, SONGUO S E N. Separation of abscisic acid, indole-3-acetic acid, gibberellic acid in 99 R (Vitis berlandieri×Vitis rupestris) and rose oil (Rosa damascena Mill.) by reversed phase liquid chromatography[J]. Turk J Chem, 2004, 28: 603-610.
[25]	吕长文.不同类型甘薯生理特性与淀粉代谢及产量调控研究[D].重庆: 西南大学, 2011. http://d.wanfangdata.com.cn/Thesis/Y1883288 LU C W.Study on Physiological Characteristics, Starch Metabolism and Yield Regulation of Different Types of Sweet Potato[D].ChongQing: Southwest University Dissertation 2011.(in Chinese) http://d.wanfangdata.com.cn/Thesis/Y1883288
[26]	段院生, 杨莉.甘薯块根生长调控研究进展[J].安徽农业科学, 2008, 36(2):14030-14032, 14083. http://d.old.wanfangdata.com.cn/Periodical/ahnykx200832052 DUAN Y S, YANG L.Research progress on tuber root growth regulation of sweet potato[J]. Anhui Agricultural Sciences, 2008, 36(2):14030-14032, 14083.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ahnykx200832052
[27]	MATSUO T, MITSUZONO H.Variation in the levels of major free cytokinins and free abscisic acid during tuber development of Sweetpotato[J].Journal of Plant Growth Regulation, 1988, 7:249-258. doi: 10.1007/BF02025267
[28]	XU X, vanLAMINEREN A AM, VERMEER E, et al. The role of gibberellin, absicisic acid, and sucrose in the regulation of potato tuber formation in vitro[J].Plant Physiology, 1998, 117(2):575-584. doi: 10.1104/pp.117.2.575
[29]	潘瑞炽.植物生理[M].北京:高等教育出版社, 1991: 18-21. PAN R Z. Plant Physiology[M]. Beijing:Higher Education Press, 1991: 18-21.(in Chinese)
[30]	MELIS R.薯类作物的成块作用和激素[J].国外农学杂粮作物, 1985(2):19-23. http://www.cnki.com.cn/Article/CJFDTotal-GNZL198502006.htm MELIS R. Blocking Effect and Hormone of Potato Crops[J].Foreign Agricultural Crops, 1985(2):19-23.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-GNZL198502006.htm

施引文献

资源附件(0)

访问统计

点击查看大图

图(5) / 表(3)

计量

文章访问数: 1847
HTML全文浏览量: 371
PDF下载量: 34
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

计量

Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

计量

目录

留言板

山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

作者简介: 敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者: 霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

计量

出版历程

Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

计量

出版历程

目录

作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)