芒果壮铗普瘿蚊为害对芒果叶片挥发物的影响

蔡鸿娇; 陈峰

doi:10.19303/j.issn.1008-0384.2019.02.013

芒果壮铗普瘿蚊为害对芒果叶片挥发物的影响

doi: 10.19303/j.issn.1008-0384.2019.02.013

蔡鸿娇^1,,
陈峰^2, ,

1.
集美大学水产学院, 福建厦门 361021
2.
福建省农业科学院植物保护研究所, 福建福州 350013

基金项目:

福建省自然科学基金 2016J01710

福建省科技计划项目——省属公益类科研院所基本科研专项 2018R1025-6

详细信息

作者简介:
蔡鸿娇(1977-), 女, 博士, 副教授, 研究方向:昆虫生态与害虫治理(E-mail:caihongjiao@jmu.edu.cn)

通讯作者:
陈峰(1979-), 男, 副研究员, 研究方向:农药残留分析与植物挥发物(E-mail:178143565@qq.com)

中图分类号: S433
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出版历程
- 收稿日期: 2019-01-02
- 修回日期: 2019-02-06
- 刊出日期: 2019-02-28

Changes on Composition of Volatile Organic Compounds in Mango Leaves Induced by Procontarinai robusta Infestation

CAI Hong-jiao^1
,,
CHEN Feng^{2
, ,}

1.
Fishery college, Jimei University, Xiamen, Fujian 361021, China
2.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 目的探讨芒果壮铗普瘿蚊为害对芒果叶片挥发物的影响，为瘿蚊防治提供参考依据。方法采用顶空固相微萃取（SPME）及气质联用仪（GC-MS）技术分析芒果壮铗普瘿蚊为害后芒果叶片挥发性物质和相对含量的变化。结果健康叶片和虫伤叶分别含有65种和66种挥发物成分，其中45个组分相同，主要包括石竹烯、α-荜澄茄烯、十八碳烯、蒈烯、水芹烯、β-月桂烯、蒎烯等。瘿蚊为害后挥发物主要为萜烯类31种和芳香族化合物13种，相对含量分别高达51.41%和41.10%，酯类物质相对含量明显高于健康叶片，成分也发生改变。虫伤叶挥发物除了萘和薁之外，主要物质包括甲基-4-（1-甲基亚乙基）-环己烯（24.32%）、α-荜澄茄烯（8.92）、3-蒈烯（2.23%）、乙酸酯3-己烯-1-醇（2.23%）、4-蒈烯（1.06%）、柠檬烯（1.04%）、a-石竹烯（1.46%）。极微量物质包括辛醇、兰桉醇、斯巴醇、表蓝桉醇、长香茅醇，香豆素类化合物只在健康叶片中检测到，马兜铃烯、法呢烯和一些特殊萘和薁芳香族化合物只在虫伤叶中检测到。结论芒果叶片的主要挥发性物质为芳香族类和萜烯类化合物，瘿蚊为害后导致挥发物质和含量发生明显改变。
- 芒果壮铗普瘿蚊 /
- 顶空固相微萃取 /
- GC-MS /
- 挥发物 /
- 虫伤叶
Abstract: Objective To identify the variations on volatile organic compounds (VOCs) in mango leaves due to infestation by Procontarinai robusta for future study to mitigate or control the disease caused by the midge. Method The VOCs in healthy leaves from a mango tree were compared with those in the midge-infested leaves by using headspace solid-phase microextraction (SPME) combined with gas chromatography mass spectrometry (GC–MS). Result The healthy leaves were found to contain 65 VOCs, while the diseased ones 66 VOCs. Between them, 45 were identical which included caryophyllene, α-cyanene, octadecene, decene, hydrocelene, β-Myrcene, and terpenes. In the diseased leaves, 31 terpenes contributed 51.41%, while 13 aromatic compounds made up 41.10%, of the total. Besides naphthalene and azulene compounds, methyl-4-(1-methylethylidene)-cyclohexene comprised 24.32%, α-cubebene 8.92%, 3-carene 2.23%, acetate 3-hexen-1-ol 2.23%, 4-carene 1.06%, limonene 1.04%, and α-caryophyllene 1.46%. The content of esters was much higher in the midge-infested leaves than that of the healthy counterparts, and the composition also differed from each other. Minute amounts of substances, such as octanol, lanthanol, spartanol, epichlorohydrin, long citronellol and coumarins, were detected in the healthy leaves but not in the infested samples. Whereas, aristoloene, farnesene and some special naphthalene and azulene aromatic compounds were found only in the midge-infested leaves. Conclusion The VOCs in mango leaves mainly consist of aromatic components and terpenes, the composition and content of which were obviously changed after Procontarinairobusta damage.
- Procontarinai robusta /
- HS-SPME /
- GC-MS /
- volatile organic compounds /
- midge-infested mango leaves

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图 1 芒果健康叶片挥发物总离子

Figure 1. Total ion current chromatograms of volatiles in healthy mango leaves

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图 2 芒果壮铗普瘿蚊为害后芒果叶片挥发物总离子

Figure 2. Total ion current chromatograms of volatiles in midge-infested mango leaves

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表 1 健康叶片和虫伤叶挥发性化合物数量及其相对含量

Table 1. Number of volatiles and relative contents (%) released from midge-infested mango leaves

序号 Number	化合物 Compound	健康叶片Healthy leaves		虫伤叶Midge-infested leaves
序号 Number	化合物 Compound	数目 Number	相对含量 Relative content/%	数目 Number	相对含量 Relative content/%
1	芳香族化合物Aromatic compounds	16	29.79	13	41.10
2	萜烯类Tetpenes	27	50.64	31	51.41
3	醇类Alcohols	9	1.05	4	0.96
4	酯类Esters	4	0.07	4	2.30
5	酮类Ketones	1	0.11	1	0.10
6	烃类Hydrocarbons	7	1.89	8	2.78
7	醛类Aldehydes	2	1.10	2	0.78
8	酸类Acids	2	0.25	5	0.80

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表 2 芒果叶片挥发性主要物质成分鉴定

Table 2. Major volatiles in mango leaves

序号 Number	化合物 Compound	相对含量Relative content/%
序号 Number	化合物 Compound	健康叶片 Healthy leaves	虫伤叶 Insect-infested leaves
1	α-荜澄茄油烯a-Cubebene	5.03	8.92
2	3-蒈烯3-Carene	3.01	2.23
3	4-蒈烯4-Carene	1.98	1.60
4	柠檬烯D-Limonene	1.10	1.04
5	α-石竹烯α-Caryophyllene	0.89	1.46
6	α-水芹烯α-Phellandrene	0.84	0.37
7	β-水芹烯β-Phellandrene	0.44	0.06
8	月桂烯β-Myrcene	0.85	0.87
9	十八碳烯1, 3, 6-Octatriene, 3, 7-dimethyl-,	0.57	0.62
10	薄荷脑烯1, 3, 8-p-Menthatriene	0.31	0.26
11	α-蒎烯α-Pinene	0.39	0.52
12	β-蒎烯β-Pinene	0.02	0.01
13	β-愈创木烯β-Guaiene	0.54	-
14	马兜铃烯(-)-Aristolene	-	2.03
15	法呢烯α-Farnesene	-	0.08
16	1-甲基-4-(1-甲基亚乙基)-环己烯，Cyclohexene, 1-methyl-4-(1 - methylethylidene)-	31.60	24.31
17	化合物薁1H-Cycloprop[e]azulene, decahydro-1, 1, 7-trimethyl-4-methylene-，[1aR-(1a.alpha., 4a.alpha., 7.alpha., 7a. beta., 7b.alpha.)]-	14.70	11.40
18	乙酸酯3-己烯-1-醇，3-Hexen-1-ol, acetate,	0.61	2.23
19	化合物萘Naphthalene, 1, 2, 3, 5, 6, 8a-hexahydro-4, 7-dimethyl-1-(1-methylethyl)-, (1S-cis)-	3.42	4.91
20	化合物萘Naphthalene, decahydro-4a-methyl-1-methylene-7-(1-methylethenyl)-, [4aR-(4a.alpha., 7.alpha., 8a.beta.)]-	6.46	5.56
21	1H-Cycloprop[e]azulene, 1a, 2, 3, 4, 4a, 5, 6, 7b-octahydro-1, 1, 4, 7-tetramethyl-, [1aR-(1a.alpha., 4.alpha., 4a.beta., 7b.alpha.)]-	10.95	11.59
22	喇叭茶醇Ledol	0.05	0.04
23	牛儿烯，双环吉马烯Bicyclogermacrene	0.23	0.31
24	1-甲基-4-苯(1-甲基乙烯基)Benzene, 1-methyl-4-(1-methylethenyl)-	1.48	1.98
25	β-律草烯β-Humulene	-	0.07
26	2-戊烯-1-醇，2-Penten-1-ol, (Z)-	-	0.02
27	1-乙烯基-1-甲基-2, 4-双(1-甲基乙烯基)-环己烷，[1s-(1.α，2.β，4.β.)]Cyclohexane, 1-ethenyl-1-methyl-2, 4-bis(1-methylethenyl)-, [1S-(1.alpha., 2.beta., 4.beta.)]-	-	2.16
28	甲基水杨酸Methyl salicylate	-	0.40
29	α-Panasinsen	0.63	0.51
30	硫氰酸二酯Thiocyanic acid carbazol-3, 6-diyl ester	0.02	-
注：表中“-”表示该成分未被检测到。 Note:“-” indicates non-detection.

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