矮壮素对小叶楠抗寒生理的影响

费越; 白志波; 牛学舫; 胡蝶; 刘刚

doi:10.19303/j.issn.1008-0384.2019.01.016

矮壮素对小叶楠抗寒生理的影响

doi: 10.19303/j.issn.1008-0384.2019.01.016

费越^1,,
白志波¹,
牛学舫²,
胡蝶¹,
刘刚^1, ,

1.
长江大学楠木种质资源评价与创新中心, 湖北荆州 434025
2.
河北省廊坊市时代广场管理处, 河北廊坊 065000

基金项目:

湖北省自然科学基金项目 2017CFB390

湖北省科技支撑计划项目 2013BBB24

详细信息

作者简介:
费越(1994-), 女, 硕士研究生, 研究方向:园林植物与观赏园艺(E-mail:763938468@qq.com)

通讯作者:
刘刚(1989-), 男, 硕士, 研究方向:园林植物种质资源评价与利用(E-mail:616003@yangtzeu.edu.cn)

中图分类号: S792.24
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- 被引次数: 0
出版历程
- 收稿日期: 2018-09-09
- 修回日期: 2018-10-19
- 刊出日期: 2019-01-28

Effect of Chlormequat on Cold-resistance of Phoebe microphylla

FEI Yue^1
,,
BAI Zhi-bo¹,
NIU Xue-fang²,
HU Die¹,
LIU Gang^{1
, ,}

1.
Center of Phoebe Germplasm Resources Evaluation and Innovation, Yangtze Uinivesity, Jingzhou, Hubei 434025, China
2.
Management Office of Times Square, Langfang, Hebei 065000, China

摘要

摘要: 目的探讨不同浓度矮壮素（chlormequat chloride，CCC）溶液对小叶楠幼苗叶片组织生理的影响，探究CCC对小叶楠幼苗的抗寒机制，为人工抚育小叶楠提供科学依据。方法以小叶楠（Phoebe microphylla）幼苗为材料，采取室内控温系统模拟低温胁迫条件，研究CCC处理对低温胁迫下小叶楠幼苗可溶性蛋白含量（SP）、丙二醛含量（MDA）、相对电导率（REC）、超氧化物歧化酶活性（SOD）、过氧化物酶活性（POD）以及过氧化氢酶活性（CAT）的影响。结果低温条件下喷施CCC可使小叶楠幼苗叶片中SP含量增加，喷施50 mg·L^-1 CCC时，SP含量最高；在0℃和-2.5℃条件下，喷施100 mg·L^-1 CCC可有效降低MDA含量；喷施50 mg·L^-1 CCC使小叶楠叶片REC值降低；在-2.5℃时，喷施100 mg·L^-1 CCC溶液的小叶楠幼苗SOD、POD和CAT活性最强。结论通过综合分析各抗寒指标，喷施100 mg·L^-1 CCC溶液时隶属函数评价最高，说明CCC能有效提高小叶楠的抗寒能力。
- 小叶楠 /
- 矮壮素 /
- 丙二醛 /
- 可溶性蛋白 /
- 保护酶系统
Abstract: Objective Effect of chlormequat application on low-temperature tolerance of Phoebe microphylla was studied. Method Under simulated conditions, seedlings of P. microphylla were subjected to low-temperature stress with treatments of chlormequat chloride (CCC) in different concentrations. The contents of soluble protein (SP) and malondialdehyde (MDA), as well as the relative electrical conductivity (REC) and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the seedlings were determined. Result After spraying with CCC solutions, SP in leaves increased and reached a maximum when 50 mg/L of CCC was applied; MDA reduced when 100 mg·L^-1 of CCC was applied at 0℃ or -2.5℃; REC declined at the presence of 50 mg·L^-1 of CCC; and, the activities of SOD, POD and CAT peaked at the spraying of 100 mg·L^-1 of CCC at -2.5℃. Conclusion Based on the above mentioned cold resistance indicators, the CCC application on the seedlings at 100 mg·L^-1 scored the highest among all tested treatments on the membership function evaluation. It was considered effective for improving the cold resistance of P. microphylla.
- Phoebe microphylla /
- chlormequat /
- malondialdehyde /
- soluble protein /
- protective enzymes

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图 1 不同温度条件下喷施不同质量浓度CCC对小叶楠的影响

注：A、B、C分别表示在不同温度条件下，施加0、50、100、150 mg·L^-1的CCC处理结果。A为0℃条件下，施加4种质量浓度的CCC处理结果；B为-2.5℃条件下，施加4种质量浓度的CCC处理结果；C为-5℃条件下，施加4种质量浓度的CCC处理结果。

Figure 1. Effects of CCC concentration on P. microphylla at low temperatures

Note: A indicates treatments of 0, 50, 100 and 150 mg·L^-1CCC spraying at 0℃; B, treatments at-2.5℃; C, treatments at-5℃.

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表 1 不同低温条件及矮壮素用量处理小叶楠的生理指标变化

Table 1. CCC applications on physiological indices of P. microphylla at low temperatures

CCC质量浓度 CCC mass concentration/ (mg·L^-1)	可溶性蛋白Soluble protein/(mg·g^-1)			丙二醛Malonaldehyde/(μmol·g^-1)			相对电导率Relative electrolytic leakage/%			超氧化物歧化酶Superoxide dismutase/(U·g^-1)			过氧化物酶Peroxidase/(U·g^-1·min^-1)			过氧化氢酶Catalase/(U·g^-1·min^-1)
CCC质量浓度 CCC mass concentration/ (mg·L^-1)	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃	0℃	-2.5℃	-5℃
对照CK	2.02±0.19Ab	1.47±0.20Bc	1.43±0.19Bb	0.05±0.002Ab	0.04±0.002Bb	0.05±0.002Ab	1.22±0.10Aa	1.05±0.10ABab	0.86±0.10Ba	3459.41±340.29Ba	4349.65±338.17Aab	4591.30±343.49Aa	470.25±39.00Aa	378.30±40.51Bb	344.59±38.52Bb	0.71±0.09Bb	1.03±0.11Ab	0.78±0.10Bc
50	2.97±0.20Aa	2.27±0.23Ba	2.35±0.22Ba	0.04±0.001Ac	0.04±0.001Ab	0.04±0.001Ac	1.04±0.05Ab	0.95±0.05ABb	0.87±0.06Ba	2879.00±343.44Ba	3801.92±343.93Ab	4075.79±350.29Aab	343.11±37.46Bb	317.03±39.39Bb	445.72±38.73Aa	1.07±0.05Aa	0.87±0.06Bbc	1.04±0.05Aa
100	1.68±0.12Bc	2.03±0.14Aab	2.06±0.12Aa	0.03±0.002Bd	0.04±0.002Ab	0.03±0.002Bd	1.18±0.06Aa	1.10±0.06Aa	0.98±0.06Ba	3275.29±472.62Ba	4800.73±489.72Aa	3592.49±476.87Bb	398.23±44.43Bab	555.96±47.49Aa	473.28±45.51ABa	0.93±0.10Ba	1.26±0.10Aa	1.01±0.11Bab
150	1.91±0.17Bbc	1.73±0.17Bbc	2.32±0.19Aa	0.07±0.002Ba	0.08±0.002Aa	0.07±0.002Ba	1.15±0.06Aab	1.01±0.05Bab	0.95±0.06Ba	2770.21±491.53Ba	4397.98±452.31Aab	3383.06±460.08Bb	315.55±51.46Bb	367.55±53.43Bb	494.69±56.46Aa	0.65±0.05Bb	0.81±0.06Ac	0.86±0.07Abc
注：表中数据为平均值±标准误；大写字母表示相同矮壮素质量浓度不同温度处理之间差异显著(P < 0.05)，小写字母表示相同温度不同矮壮素质量浓度处理之间差异显著(P < 0.05)。 Note: the data in the table are average value ±standard error; the capital letters mean significant differences between treatments with the same dwarf concentration at different temperatures (P < 0.05), and the lowercase letters showed significant differences between the treatments at the same temperature and different concentrations of dwarfs (P < 0.05).

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表 2 外源CCC对小叶楠抗寒指标的隶属函数评价

Table 2. Membership function evaluation on cold resistance indicators of P. microphylla upon exogenous CCC applications

温度 Temperature /℃	质量浓度 Mass concentration /(mg·L^-1)	隶属函数值Membership function value
温度 Temperature /℃	质量浓度 Mass concentration /(mg·L^-1)	SP含量 SP content	MDA量 MDA content	REC	SOD活性 SOD activity	POD活性 POD activity	CAT活性 CAT activity	隶属函数平均值 Membership function average	排序 Sort
0	0	0.27	0.53	1.00	1.00	1.00	0.14	0.66	4
	50	1.00	0.78	0.00	0.14	0.17	1.00	0.52	6
	100	0.00	1.00	0.84	0.69	0.52	0.67	0.62	5
	150	0.17	0.00	0.58	0.00	0.00	0.00	0.13	12
-2.5	0	0.00	0.82	0.67	0.58	0.24	0.51	0.47	8
	50	1.00	1.00	0.00	0.00	0.00	0.18	0.36	9
	100	0.70	0.97	1.00	1.00	1.00	1.00	0.95	1
	150	0.31	0.00	0.39	0.59	0.22	0.00	0.25	11
-5	0	0.00	0.53	0.00	1.00	0.00	0.00	0.26	10
	50	1.00	0.85	0.16	0.57	0.68	1.00	0.71	3
	100	0.66	1.00	1.00	0.17	0.82	0.88	0.76	2
	150	0.95	0.00	0.73	0.00	1.00	0.33	0.50	7

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