基于无患子科ITS序列探讨龙荔的系统发育

姜帆; 陈秀萍; 郑少泉

doi:10.19303/j.issn.1008-0384.2019.01.006

基于无患子科ITS序列探讨龙荔的系统发育

doi: 10.19303/j.issn.1008-0384.2019.01.006

姜帆^{1, 2,},
陈秀萍^{1, 2},
郑少泉^{1, 2, ,}

1.
福建省农业科学院果树研究所, 福建福州 350013
2.
福建省龙眼枇杷育种工程技术研究中心, 福建福州 350013

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 2015R104-8

国家现代农业产业技术体系建设专项 CARS-32-06

福建省农业科学院创新团队 STIT2017-1-4

福建省农业科学院青年英才计划 C2017-04

详细信息

作者简介:
姜帆(1977-), 男, 副研究员, 主要从事龙眼枇杷种质资源、育种和品质生物技术研究(jiangfan006@126.com)

通讯作者:
郑少泉(1964-), 男, 研究员, 主要从事果树育种、种质资源与品质生物技术研究(E-mail:zsq333555@163.com)

中图分类号: S667.2
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出版历程
- 收稿日期: 2018-10-07
- 修回日期: 2018-11-15
- 刊出日期: 2019-01-28

ITS-based Phylogeny of Dimocarpus confinis

JIANG Fan^{1, 2
,},
CHEN Xiu-ping^{1, 2},
ZHENG Shao-quan^{1, 2
, ,}

1.
Fruit Research Institute, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China
2.
Fujian Fruit Breeding Engineering Technology Research Center for Longan & Loquat, Fuzhou, Fujian 350013, China

摘要

摘要: 目的利用无患子科间隔转录区序列（ITS）探讨了龙荔的系统发育地位。方法通过无患子科27个属62个种ITS序列信息序列比对和系统发育分析，探讨龙荔和龙眼、荔枝之间的系统进化关系。结果基于ITS序列信息可以清楚区分无患子科内不同的种属，其属间ITS序列相似度为（91.0±4.4）%、种间为（96.3±2.8）%。龙荔与龙眼、荔枝之间的ITS序列同源性均为92%，低于龙眼与荔枝间的同源性（93%），但高于和红毛丹的同源性（91%），其ITS序列中有4.5%的碱基位点与龙眼和荔枝均不同，主要表现为T和C互换、A和G互换，共占序列差异位点的56.5%。以韶子属红毛丹为外类群的系统发育树显示，龙荔、荔枝、龙眼之间的进化发育明显，龙荔最早自成分支，其次是荔枝，最后是龙眼。结论 ITS序列信息分析结果支持龙荔作为独立属的分类可能。
- 龙荔 /
- 种系进化 /
- ITS
Abstract: Objective To establish the phylogeny of longli, Dimocarpus confinis, based on its nuclear encoded internal transcribed spacer (ITS) sequences. Method The ITS sequences of 27 genera and 62 species of Sapindaceae were collected to examine the relationship among longli, longan (Dimocarpus longan) and litchi (Litchi chinensis). Result The genera in Sapindaceae family could be clearly classified by ITS sequence. The homology among the genera was(91.0±4.4)%, and among the species (96.3±2.8)%. The ITS sequence homology between longli and longan or litchi was 92%, which was lower than that between longan and litchi at 93% but higher than that between longli and rambutan (Nephelium lappaceum L.) at 91%. Four and half percent of the longli ITS sequence differed from those of longan and litchi. The differences were mainly changing between T and C or A and G that accounted for 56.5% of the total. Based on the phylogenetic tree of rambutan as the outgroup, the evolution of longli was shown to be the earliest to branch out, followed by litchi and longan. Conclusion The result suggested classifying longli as a separate genus in the family.
- Dimocarpus confinis /
- pylogeny /
- ITS sequence

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图 1 无患子科27个属的ITS聚类

Figure 1. ITS homology of 27 genera

下载: 全尺寸图片幻灯片

图 2 龙荔和龙眼、荔枝之间的同源性比较

Figure 2. Homology between longli and longan or ltichi

下载: 全尺寸图片幻灯片

图 3 龙荔和龙眼、荔枝之间的种系进化树

注：NJ法，自展1 000次。Note:Neighbour joining method, boot strapping 1 000 times.

Figure 3. Phylogeny tree of longli, longan and ltichi

下载: 全尺寸图片幻灯片

表 1 ITS序列分析用植物材料

Table 1. Plant materials employed for ITS analysis

序号 No.	属 Genus	基因编号 Genebank number
1	毛花槭Acer erianthum	EU720501.1
2	糖槭Acer saccharum	EU720502.1
3	Beguea apetala	EU720512.1
4	Beguea apetala	EU720491.1
5	Cupania dentata	EU720523.1
6	Cupania hirsuta	EU720521.1
7	Cupania rubiginosa	EU720481.1
8	Cupania scrobiculata	EU720524.1
9	Cupaniopsis anacardioides	EU720438.1
10	Cupaniopsis flagelliformis	EU720432.1
11	Deinbollia macrocarpa	EU720503.1
12	Deinbollia oblongifolia	EU720427.1
13	Deinbollia pervillei	EU720395.1
14	车桑子Dodonaea madagascariensis	EU720518.1
15	车桑子Dodonaea viscosa	EU720536.1
16	车桑子Dodonaea viscosa	EU720519.1
17	Elattostachys	EU720538.1
18	Elattostachys nervosa	EU720455.1
19	Elattostachys sp.	EU720529.1
20	Eriocoelum kerstingii	EU720539.1
21	Eriocoelum microspermum	EU720540.1
22	Filicium decipiens
23	Filicium longifolium	EU720466.1
24	Filicium thouarsianum	EU720541.1
25	Guioa microsepala	EU720493.1
26	Guioa semiglauca	EU720546.1
27	Guioa villosa	EU720458.1
28	Lepiderema hirsuta	EU720544.1
29	Lepiderema pulchella	EU720435.1
30	鳞花木Lepisanthes ferruginea	EU720454.1
31	鳞花木Lepisanthes rubiginosa	EU720449.1
32	滇赤才Lepisanthes senegalensis	EU720446.1
33	Llagunoa mollis	EU720492.1
34	Llagunoa nitida	EU720482.1
35	Macphersonia chapelieri	EU720486.1
36	Macphersonia gracilis	EU720459.1
37	Mischocarpus exangulatus	EU720550.1
38	Mischocarpus grandissimus	EU720437.1
39	Mischocarpus pentapetalus	EU720470.1
40	Mischocarpus pyriformis	EU720460.1
41	Molinaea petiolaris	EU720554.1
42	Molinaea sp.	EU720.510.1
43	红毛丹Nephelium lappaceum	EU720401.1
44	Paranephelium macrophyllum	EU720451.1
45	Paranephelium xestophyllum	EU720420.1
46	Paullinia subauriculata	EU720494.1
47	Plagioscyphus aff.	EU720555.1
48	Sarcopteryx martyana	EU720426.1
49	Sarcopteryx reticulata	EU720421.1
50	Serjania communis	EU720472.1
51	Serjania glabrata	EU720557.1
52	Talisia angustifolia	EU720558.1
53	Talisia nervosa	EU720474.1
54	Talisia obovata	EU720485.1
55	Tina isaloensis	EU720520.1
56	Tina striata	EU720509.1
57	Toechima erythrocarpum	EU720431.1
58	Toechima plurinerve	EU720452.1
59	Toechima tenax	EU720456.1
60	Toechima erythrocarpum	EU720431.1
61	Toechima plurinerve	EU720452.1
62	Toechima tenax	EU720456.1
63	荔枝Litchi chinensis Yuan s.n.	EU720400.1
64	7811龙眼Dimocarpus longan vary 7811	EF532334.1
65	石硖龙眼Dimocarpus longan vary shixia	EF488973.1
66	十月龙眼Dimocarpus longan varyshiyuelongyan	EF532338.1
67	云南8号龙眼Dimocarpus longan vary yunnan No8	EF532331.1
68	贵州6号龙眼Dimocarpus longan vary guizhou No6	EF532333.1
69	灵龙龙眼Dimocarpus longan varylinglong	EF532329.1
70	依多龙眼Dimocarpus longan varyEdor	EF532337.1
71	香枝龙眼Dimocarpus longan vary xiangzhilongyan	/
72	京龙眼Dimocarpus longan vary.jinglongyan	/
73	万隆安龙眼Dimocarpus longan vary Band-ungan	EF532340.1
74	青壳宝圆龙眼Dimocarpus longan.vary qingkebaoyuan	EF488971.1
75	龙荔Dimocarpus confinis	EF532336.1

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参考文献(18)

[1]	邱武陵, 章恢志.中国果树志·龙眼批把卷[M].北京:中国林业出版社, 1996. QIU W L, ZHANG H Z.China Fruit Graphy:Longan and Loquat Tomus[M]. Beijing:China Forestry Publishing House, 1996.(in Chinese)
[2]	潘丽梅, 朱建华, 刘冰浩, 等.广西龙虎山自然保护区龙荔种群生命表分析[J].园艺学报, 2011, 38(7):1349-1355. http://d.old.wanfangdata.com.cn/Periodical/yyxb201107018 PAN L M, ZHU J H, LIU B H, et al.Life Table Analysis of Dimocarpus confinis Population in Guangxi Longhushan Nature Reserve[J]. Acta Horticulturae Sinica, 2011, 38(7):1349-1355.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201107018
[3]	陈秀萍, 蒋际谋, 许家辉, 等.不同砧木对龙眼生长的影响试验[J].中国南方果树, 2006, 35(5):21-22. doi: 10.3969/j.issn.1007-1431.2006.05.013 CHE X P, JIANG J M, XU J H, et al.Reseach of longan development based on different storcks[J]. South China Fruits, 2006, 35(5):21-22.(in Chinese) doi: 10.3969/j.issn.1007-1431.2006.05.013
[4]	陆贵锋, 朱松生, 黄川, 等.龙荔嫁接龙眼、荔枝的试验初报[J].中国南方果树, 2016, 45(5):148-149. http://www.cnki.com.cn/Article/CJFDTOTAL-FRUI201605047.htm LU G F, ZHU S S, HUANG C, et al.research of grafting between Longli and Longan and Litchi[J]. South China Fruits, 2016, 45(5):148-149.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-FRUI201605047.htm
[5]	刘冰浩, 徐宁, 朱建华, 等.广西龙眼种质耐寒性的CTR值鉴定[J].西南农业学报, 2006, 19(4):668-671. doi: 10.3969/j.issn.1001-4829.2006.04.029 LIU B H, XU N, ZHU J H, et al.The CTR identification of chilling-tolerance of Longan germplasm in Guangxi[J]. Southwest China Journalof Agricultural Science, 2006, 19(4):668-671.(in Chinese) doi: 10.3969/j.issn.1001-4829.2006.04.029
[6]	姜帆, 高慧颖, 陈秀萍, 等.龙眼属rDNA的ITS序列分析[J].果树学报, 2008, 25(2): 262- 268. http://d.old.wanfangdata.com.cn/Periodical/gskx200802024 JIANG F, GAO H Y, CHE X P, et al.Analysis of ITS sequences of rDNA in Dimocarpus plants[J]. Journalof Fruit Science, 2008, 25(2): 262- 268.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gskx200802024
[7]	高慧颖, 姜帆, 陈秀萍, 等.不同地域的代表性基因型龙眼RAPD分析[J].福建林业科技, 2007, 34(1): 67-71, 88. doi: 10.3969/j.issn.1002-7351.2007.01.017 GAO H Y, JIANG F, CHE X P, et al.Random Amplification Polymorphic DNA (RAPD) Analysis of the Typical Longan Genotypes from Different Origins[J]. Journal of Fujian Forestry Science and Technology, 2007, 34(1): 67-71, 88.(in Chinese) doi: 10.3969/j.issn.1002-7351.2007.01.017
[8]	彭宏祥, 曹辉庆, 朱建华, 等.利用AFLP分子标记对广西荔枝优稀种质遗传多样性及分类研究[J].西南农业学报, 2006, 9(1): 108-111. doi: 10.3969/j.issn.1001-4829.2006.01.026 PENG H X, CAO H Q, ZHU J H, et al.Studies on the application of AFLP molecular markers on genetic diversity and classification of good and rare litchi resources in Guangxi[J]. Southwest China Journalof Agricultural Science, 2006, 9(1): 108-111.(in Chinese) doi: 10.3969/j.issn.1001-4829.2006.01.026
[9]	易干军, 谭卫萍, 霍合强, 等.龙眼品种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报, 2003, 30(3): 272-276. doi: 10.3321/j.issn:0513-353X.2003.03.005 YI G J, TAN W P, HU H Q, et al.Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP Analysis[J]. Acta Horticulturae Sinica, 2003, 30(3): 272-276.(in Chinese) doi: 10.3321/j.issn:0513-353X.2003.03.005
[10]	苏伟强, 黄海滨, 陆玉英, 等.龙荔与龙眼荔枝过氧化物酶同工酶分析及亲缘关系研究初报[J].广西农业科学, 1993(4): 158-159. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000000920143 SU W Q, HUANG H B, LU Y Y, et al.Studies on the Relationship of Longli and Longan by POD Analysis[J]. Agricultural Science of Guangxi, 1993(4): 158-159.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000000920143
[11]	ROBERT VenBUREN, et al.Longli is not a Hybrid of Longan and Lychee as Revealed by Genome Size Analysis and Trichome Morphology[J]. Tropical Plant Biology, Tropical Plant Biol, 2011, 4: 228-236.(in Chinese) doi: 10.1007/s12042-011-9084-3
[12]	张永福, 韩丽, 黄鹤平, 等.荔枝、龙眼及龙荔的茎解剖结构比较[J].植物科学学报, 2014, 32(6): 551-560. http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201406001 ZHANG YF, HAN L, HUANG HP et al.Comparisons of Stem Anatomical Structures among Litchi, Longan and Longli[J]. Plant Science Journal, 2014, 32(6): 551-560.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201406001
[13]	LIN T X, LIN Y, KOSHUN LSHIKI.Genetic diversity of Dimocarpus longan in China revealed by AFLP markers and partial rbcL gene sequences[J]. Scientia Horticulturae, 2005, 103(4):489-498. doi: 10.1016/j.scienta.2004.08.005
[14]	LIU C M, MEI M T.Classification of lychee cultivars with RAPD analysis[J]. Acta Horticulturae, 2005, 665: 149-159.
[15]	BUERKI S, FOREST F, ACEVEDO-RODRIGUEZ P, et al.Plastid and nuclear DNA markers reveal intricate relationships at subfamilial and tribal levels in the soapberry family (Sapindaceae)[J]. Mol Phylogenet Evol, 2009, 51 (2), 238-258. doi: 10.1016/j.ympev.2009.01.012
[16]	何婷婷, 柴军红.基于ITS基因的部分兰属序列分析[J].分子植物育种, 2017, 15(1): 52- 57. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201701008 HE T T, CAI J H.Sequence Analysis of Cymbidium Based on ITS Gene[J]. Molecular Plant Breeding, 2017, 15(1): 52- 57.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201701008
[17]	曹丽敏, 夏念和, 曹明, 等.中国无患子科的花粉形态及其系统学意义[J].植物科学学报, 2016, 34(6): 821-833. http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201606001 CAO LM, XIA NH, CAO M, et al.Pollen Morphology and Its Systematic Significance in Sapindaceae from China[J]. Plant Science Journal, 2016, 34(6): 821-833.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/whzwxyj201606001
[18]	PETKOVITS T, NAGY L G, HOFFMANN K, et al.Data Partitions, Bayesian Analysis and Phylogeny of the Zygomycetous Fungal Family Mortierellaceae, Inferred from Nuclear Ribosomal DNA Sequences[J]. PLoS ONE, 2011, 6(11): e27507. doi: 10.1371/journal.pone.0027507