Selection of Carrier for Dried Bacillus natto
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摘要: 为研制纳豆芽胞杆菌固体菌剂的制备工艺，以活菌数为指标，考察不同载体，配比，干燥温度等因素对固体菌剂有效活菌数的影响。结果表明：温度37℃，转速150 r·min-1，摇床上进行吸附60 min后，最优吸附载体为麦饭石，最适吸附比例为1：1，干燥温度40℃，初始有效活菌数达1.09×109 CFU·g-1，最适保藏温度25℃，保藏6个月后仍有2.9×108 CFU·g-1，活菌数残留率为20.2%。同时，麸皮、豆粕、玉米粉可为菌剂提供营养，有利于固体菌剂的长期保藏。Abstract: Preparation of a dried Bacillus natto product was studied. The carrier material, mixing ratio, and drying temperature for the making were evaluated according to the survival rate of B. natto in the dried product. The optimal process was determined to be stirring the bacterial solution with quartz monzonite as the adsorbent carrier in a mixing ratio of 1:1 at 150 r·min-1 and 37℃ for 60 min in a container prior to dehydration at 40℃. The dried product had a bacterial count of 1.09×109 CFU·g-1. After 6-month storage at 25℃, the count decreased by 20.2% to 2.9×108 CFU·g-1. Inclusion of wheat bran, soymeal and/or corn meal in the mix would provide nutrients for B. natto resulting in aprolonged shelf life of the dried product.
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Key words:
- Bacillus natto /
- dried bacteria /
- carrier /
- quartz monzonite /
- adsorption
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图 1 不同吸附比对有效活菌数的影响
注：图中不同小写字母表示处理间差异显著(P＜0.05)。图 2同。
Figure 1. Effect of bacteria/carrier ratio on viable bacteria count in dried product
图 2 不同干燥温度对有效活菌数的影响
Figure 2. Effect drying temperature on viable bacteria count in dried product
图 3 25℃保存条件下不同载体固化菌剂有效活菌数变化
Figure 3. Viable bacteria count in dried products of different carriers stored at 25℃
图 4 4℃保存条件下不同载体固化菌剂有效活菌数变化
Figure 4. Viable bacteria count in dried products of different carriers stored at 4℃
表 1 不同载体吸附效果比较
Table 1. Bacterial adsorptions on different carriers
麦饭石 活性炭 麸皮 玉米粉 豆粕 初始液体菌剂 有效活菌数/(108CFU·g-1) 10.9±0.436a 1.02±0.433a 8.44±0.087b 8.21±0.106b 7.94±0.599b 11.7 吸附率/% 93.2 87.2 72.1 70.2 67.9 含水量/% 9.11 9.03 9.44 9.61 9.74 注：数据后不同小写字母表示处理间差异显著(P＜0.05)。 
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